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Topic: Radioimmune assay

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	Radioimmune assay and characteristics of antibodies to macromomycin (NSC 170105) -- Winkelhake and Buckmire 37 (4): ...
		Radioimmune assay and characteristics of antibodies to macromomycin (NSC 170105)
		the sequential saturation assay was linear on a logit-log plot and
		The radioimmune assay was suitable for measuring macromomycin
	cancerres.aacrjournals.org /cgi/content/abstract/37/4/1197 (228 words)

	Technology Primer
		This highly sensitive assay enables monitoring of an analyte’s interaction with patient specimens by the presence or absence of fluorescence.
		Many determinations in clinical assays are based upon analyte detection by the measure of absorbed radiant energy through specific wavelength filters.
		The viral cytopathic assay is a technique used to detect neutralizing antibodies (NAbs) to interferon (IFN) by directly evaluating cell survival in the presence of IFN.
	www.athenadiagnostics.com /site/content/technology/tech_primer.asp (1199 words)

	witts.org
		In ELISA assay, a reaction in the form of oxidation of the bound enzyme-labelled antibody, produces a colour formation that is proportional to the amount of antigen or antibody present in the sample.
		Radioimmune Assay is very similar to ELISA method, except that it uses radio-isotopes instead of enzyme-labelled antibody to detect the antigen/antibody complex.
		The viral cytopathic assay is a technique used to detect neutralizing antibodies to interferon by directly evaluating cell survival in the presence of interferon.
	www.witts.org /diagnostic_2_jun04/diagno_ontoexclnce.htm (648 words)

	Compositions and method for simultaneous multiple array of analytes using radioisotope chelate labels - Patent 4672028
		The composition according to claim 1 useful in a simultaneous assay wherein the analyte portion of one or more of said stable coordinated compounds is formed from a steroid, a protein, a peptide, a carbohydrate or a drug, and wherein each radioisotope in said composition is different.
		The composition according to claim 4 useful in a simultaneous assay wherein the analyte portion of one or more of said stable coordinated compounds is formed from thyroid stimulating hormone or follicle stimulating hormone, and wherein each radioisotope in said composition is different.
		The composition according to claim 6 useful in a simultaneous assay wherein the analyte portion of one or more of said stable coordinated compounds is formed from a chelating agent selected from ethylenediaminetetraacetic acid, ethylene dinitrilotetraacetic acid, diethylenetriaminepentaacetic acid or derivatives thereof, and wherein each radioisotope in said composition is different.
	www.freepatentsonline.com /4672028.html (5417 words)

	Bioline International Official Site (site up-dated regularly)
		This report is an attempt to summarize in brief the data accumulated to date on these points and to indicate the directions for future applications of these specific monoclonal antibodies for identification of leishmanial isolates.
		Characterization of the Leishmania was performed with an indirect radioimmune binding assay (RIA) using whole parasite lysates as antigen.
		However, variation in the sensitivity of the test may occur due to the type of screening assay used (i.e., immunofluorescence, RIA or the ELISA technique).
	www.bioline.org.br /request?oc96006 (2945 words)

	Immunology Contract Laboratory offers Cellular Assays, Immunoassays, Monoclonal Antibody Production, Purification, ...
		A high throughput assay typically is not complicated, must be very reproducible and have a high signal-to-noise ratio in order to minimize false positives.
		Additionally, stabilizing the assay components so that production of the completed assay for use in the screens is an objective when developing the initial configuration of the assay.
		Sera are tested for antibody titer in an ELISA assay (or with the investigator's screening method) and if titers are high, hyperimmunization is performed and spleen cells are isolated and fused to myeloma cells with polyethylene glycol (PEG).
	www.marinbio.com /immunology2.html (1828 words)

	[No title]
		The Radioimmune Assay (RIA) is used to detect trace amounts of urine Cortisol, Testosterone, and FSH, as well as the plasma Catecholamines.
		Assays involving ELISA methodology include the ability to detect urinary Estrones, Melatonin and Creatinines.
		The lab manager works with researchers involved in grant preparation in order to determine projected assay costs, technician time, sample storage, data analysis and use of shared lab resources.
	www.son.washington.edu /research/internal/Labs/Services.doc (113 words)

	[No title] (Site not responding. Last check: 2007-10-25)
		Owing to the great promotion of medical knowledge, pharmacological manufacture, and instrumental technology in recent years, besides the traditional functional imaging, nuclear medicine now could provide more accurate anatomic relationship of disease, especially after the new scanner consisted with CT function was vended.
		In our hospital, there are two major jobs of nuclear medicine, including radioimmune assay and radiopharmaceutical scintigraphy.
		Radioimmune assay could evaluate some special metabolites or hormone in kinds of body fluid, and radiopharmaceutical scintigraphy could provide visible information of body status with injected very few amount of radiotracer.
	www.tsgh.ndmctsgh.edu.tw /nuc/english/e-int-introduction.htm (165 words)

	CRORA : RESUME DES PUBLICATIONS
		Monoclonal antibody 47-10/10, prepared using D80-100 virus and directed at the NS1 non-structural glycoprotein, had a significantly lower (100-fold) solid phase radioimmune assay endpoint titre for D80-141 antigen than for D80-100 antigen.
		By the indirect immunofluorescence assay, 47-10/10 had lower antibody endpoint titres against D80-141, the NGC strain and 13 (12%) of 110 Bangkok/80 isolates than to a control antibody preparation.
		These results suggest that strain D80-141 represents a second minor topotype of dengue-2 which was circulating concurrently with the major endemic topotype in Bangkok in early 1980.
	www.pasteur.fr /recherche/banques/CRORA/res5/re2945.htm (249 words)

	CXCR4/CCR5 Down-modulation and Chemotaxis Are Regulated by the Proteasome Pathway -- Fernandis et al. 277 (20): 18111 ...
		Migration Assays-- Migration assays were performed according to the procedures described previously (37).
		radioimmune precipitation assay buffer and twice with kinase buffer
		The resulting cell extracts were then assayed for CAT activity as described under "Experimental Procedures." The protein was normalized against the
	www.jbc.org /cgi/content/full/277/20/18111 (4601 words)

	UBEP 2000 Seventh Annual Undergraduate Research Symposium (Site not responding. Last check: 2007-10-25)
		The long-term goal of this study is to measure changes in amylin concentrations in rat serum under a variety of stress conditions.
		Radioimmune assay is commonly used to measure low levels of amylin, which range from 20 to 50 pM, in normal rat serum.
		Currently, we are examining the use of slot blotting and chemifluorescence techniques for the quantitation of amylin.
	lifesciences.asu.edu /ubep2000/abstracts/abst33 (186 words)

	Bioline International Official Site (site up-dated regularly)
		Prior to analysis, the samples were briefly sonicated using a bath sonicator (RAI Research, Model 250 Ultrasonic cleaner) to homogenize the antigens, and then centrifuged (2,000 x g for 10 min, at 4 C).
		Protein concentration was measured by the protein assay method of Bradford (1976), and all samples were resuspended to the same concentration before analysis.
		In studies using a dot-blot radioimmune assay, these MAbs appear to be specific for the genus Endotrypanum, but monoclonal E-2 reacted exclusively with strain M6159 of E.
	www.bioline.org.br /request?oc97013 (2391 words)

	The Detection of Insulin in Postmortem Tissues
		The assay involved injection of tissue extracts into mice and reversal of the induced hypoglycemia by glucose.
		In a forensic toxicology laboratory it is inconvenient to keep mice solely for use in an occasional insulin assay and also, the radioimmunoassay has been found to be more sensitive.
		The principle of the analysis is that of isotope dilution, the extracted insulin being used to compete with iodine-125 labeled insulin for a limited quantity of anti-insulin antibody.
	www.astm.org /JOURNALS/FORENSIC/PAGES/5195.htm (224 words)

	INCREASED PLASMA IMMUNO REACTIVE-ENDOTHELIN-1 IN NORMOTENSIVE INSULIN DEPENDENT DIABETIC PATIENTS WITH NO RENAL ... (Site not responding. Last check: 2007-10-25)
		Radioimmunoassay (RIA) was performed on reconstituted eluted samples using rabbit anti-human endothelin-1 as a primary antibody and goat anti-rabbit as a secondary antibody.
		The antibody used in this assay primarily recognizes endothelin-1, with 100% cross-reactivity.
		We used a sensitive radioimmunoassay method for assay of previously extracted plasma samples with the least possible cross-reactivity.
	www.egyptheart.org /EHJ1/EHJ1/ehj5004p078.html (2109 words)

	CRORA : RESUME DES PUBLICATIONS (Site not responding. Last check: 2007-10-25)
		Diagnosis can be aided by the history of seasonal occurrence, climate, geographic location, exposure to vectors, and age of the patient.
		The definitive diagnosis is usually made by serological tests such as neutralization, complement-fixation, hemagglutination-inhibition, and immunofluorescence, the radioimmune assay and the enzyme-linked immunosorbent assay show promise of future utility.
		It is unlikely that vaccines or anti-viral agents will have application in the near future.
	www.pasteur.fr /recherche/banques/CRORA/res5/re2636.htm (107 words)

	Enzyme-linked immunosorbent assay (ELISA) definition - Medical Dictionary definitions of popular medical terms
		Enzyme-linked immunosorbent assay (ELISA): ELISA stands for "enzyme-linked immunosorbent assay." This is a rapid immunochemical test that involves an enzyme (a protein that catalyzes a biochemical reaction).
		The substance the enzyme acts on is then added, and the amount of product measured in some way, such as a change in color of the solution.
		ELISA tests are generally highly sensitive and specific and compare favorably with radioimmune assay (RIA) tests.
	www.medterms.com /script/main/art.asp?articlekey=9100 (280 words)

	Delaware Valley Mass Spectrometry Discussion Group (Site not responding. Last check: 2007-10-25)
		Immunoassays, such as radioimmune assay (RIA), also have potential cross-reactivity with therapeutic drugs.
		Urinary free cortisol (UFF) is used extensively in the diagnosis of Cushing's disease and was the first HPLC assay to be converted.
		An HPLC assay of 150-200 samples took 36 to 48 hrs to complete and required approximately 15-20% of samples to be repeated due to some form of interference.
	science.widener.edu /svb/msdg/announce2002a.html (329 words)

	Retroviruses and multiple sclerosis. I. Analysis of seroreactivity by western blot and radioimmune assay.
		Analysis of seroreactivity by western blot and radioimmune assay.
		The state of the art may have changed since the publication date.
		We were unable to identify an antibody to either HTLV-I or HIV-1 in patients with multiple sclerosis (MS) by ELISA, by radioimmune assay, or by radioimmune precipitation techniques.
	www.aegis.com /aidsline/1990/nov/M90B0669.html (347 words)

	Hepatitis C Virus and HIV Envelope Proteins Collaboratively Mediate Interleukin-8 Secretion through Activation of p38 ...
		Using the luciferase reporter assay kit (BD Biosciences Clontech, Palo Alto, CA), the cells were lysed,
		and the luciferase reporter assay was performed as per the
		The supernatants were collected after 24 h and assayed by ELISA (Endogen) for the production of IL-8.
	www.jbc.org /cgi/content/full/278/37/35755 (6448 words)

	Radioimmunoassay for serum myoglobin -- Adams et al. 8 (4): 330 -- Annals of Clinical and Laboratory Science
		A column flow-through radioimmune assay for serum myoglobin has been developed.
		The assay uses antibodies to human or rhesus monkey myoglobin coupled to Sepharose by cyanogen bromide activation and myoglobin labeled by the Chloramine-T method.
		The labeled myoglobin is stable over two to three half-lives.
	www.annclinlabsci.org /cgi/content/abstract/8/4/330 (169 words)

	Retroviruses and multiple sclerosis. I. Analysis of seroreactivity by western blot and radioimmune assay -- Weinshenker ...
		Analysis of seroreactivity by western blot and radioimmune assay -- Weinshenker et al.
		We were unable to identify an antibody to either HTLV-I or HIV-1 in
		patients with multiple sclerosis (MS) by ELISA, by radioimmune assay, or by
	www.neurology.org /cgi/content/abstract/40/8/1251 (135 words)

	Accumulation of leukotriene C4 and histamine in human allergic skin reactions.
		Accumulation of leukotriene C4 and histamine in human allergic skin reactions.
		Skin chamber sites challenged with ragweed or grass pollen antigen (250-750 protein nitrogen units/ml) for the first hour and phosphate-buffered saline (PBS) for the next 3 h were assayed hourly and compared to sites challenged with PBS alone.
		We have demonstrated in this study that of the leukotrienes assessed LTC4 is released in the greatest quantity in situ during in vivo allergic cutaneous reactions and that it is present at such sites for at least 4 h after antigen challenge.
	www.ihop-net.org /UniPub/iHOP/gp/5110863.html (258 words)

	Comparison of three ELISA kits for the determination of antibodies to HIV-1.
		Some of these samples previously gave ambiguous results by one or more ELISA kits used in routine testing.
		Any ELISA reactive sample was verified by Immunofluorescence assay, Western blot (WB) or Radioimmune precipitation assay (RIPA).
		These results show that the IAFB kit which uses a synthesized antigen equivalent to conservatively retained structural HIV-1 proteins, offers an improved performance when tested against this panel of sera.
	www.aegis.com /aidsline/1991/jul/M9170831.html (482 words)

	docs-product_en (Site not responding. Last check: 2007-10-25)
		An Immunoaffinity Immobilized Enzyme Assay for Neomycin Phosphotransferase II in Crude Cell Extracts.
		A blot assay for detection of arginase using an arginine or canavanine enzyme conjugate
		A cell-based assay for screening the uridine 5'-diphosphate-glucuronosyltransferase 1A inhibitory potential of new chemical entities.
	millipore.com /bibliography.nsf/search?open&viewbase=Product&... (2169 words)

	Millipore - Applications Bibliography - Radioimmune Assay of Sialy transferase and N-Acetylgalactosaminyltransferase ...
		Millipore - Applications Bibliography - Radioimmune Assay of Sialy transferase and N-Acetylgalactosaminyltransferase Activities Using Specific Antibodies on a 96-Well Filtration Plate of a MultiScreen Assay System
		Radioimmune Assay of Sialy transferase and N-Acetylgalactosaminyltransferase Activities Using Specific Antibodies on a 96-Well Filtration Plate of a MultiScreen Assay System
		Please note: This information is provided to you for technical reference.
	www.millipore.com /bibliography.nsf/docs/RA03 (88 words)

	ELISA Tests - Womens Health and Medical Information on MedicineNet.com
		What is the use of an ELISA test?
		An ELISA test uses components of the immune system and chemicals to detect immune responses in the body (for example, to infectious microbes).
		They are considered highly sensitive and specific and compare favorably with other methods used to detect substances in the body, such as radioimmune assay (RIA) tests.
	www.medicinenet.com /elisa_tests/article.htm (440 words)

	Lib #: 911437 - Radioimmune Assay of Sialytransferase and N-Acetylgalactosaminyltransferase Activities Using Specific ...
		Lib #: 911437 - Radioimmune Assay of Sialytransferase and N-Acetylgalactosaminyltransferase Activities Using Specific Antibodies on a 96-Well Filtration Plate of Mulriscreen Assay System
		Radioimmune Assay of Sialytransferase and N-Acetylgalactosaminyltransferase Activities Using Specific Antibodies on a 96-Well Filtration Plate of Mulriscreen Assay System
		Read the Book on SPE Methods Development [568K] PDF
	waters.com /WatersDivision/SiteSearch/AppLibDetails.asp?LibNum=911437 (77 words)

	Energy Citations Database (ECD) - Energy and Energy-Related Bibliographic Citations
		Radioimmune assay of heterologous serum $gamma$-globulin in patients receiving rabbit antihuman thymocyte globulin
		Measurement of serum ferritin by a 2-site immunoradiometric assay
		Properties of two-site immunoradiometric (labelled antibody) assay systems
	www.osti.gov /energycitations/searchresults.jsp?Author=Bieber,+C.P. (194 words)

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