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Radioimmunoassay methods for the determination of L-triiodothyronine and thyroxine - Patent 4111656 An improved radioimmunoassay method for the in vitro determination of thyroxine in unextracted blood serum is characterized by the use of an antiserum containing antibody capable of immunoreactivity with thyroxine and prepared from an immunogen comprising a conjugate of the N-acetyl derivative of thyroxine coupled to bovine serum albumin with 1-ethyl-3-(3-dimethylaminopropyl)-carbodiimide. A radioimmunoassay method as set forth in claim 1 wherein the unbound thyroxine is separated from the antibody bound thyroxine by contacting the mixture with a relatively thin strip of a membrane consisting essentially of an ion-exchange resin for a predetermined length of time. For use in the radioimmunoassay methods of the invention, it is preferred that the antiserum be diluted to the extent that 60-75% of a tracer quantity of radioactive T3 is bound, i.e., a dilution of greater than 1:100. www.freepatentsonline.com /4111656.html   (9295 words)

Automated direct serum radioimmunoassay - United States Patent 4,108,976 Automated radioimmunoassay directly from serum, for those antigens present in serum in relatively small amounts, is accomplished by use of an immobilized immunoadsorbent having an excess of antibodies, specific to the antigen, covalently bonded to a particulate substrate. Radioimmunoassay is an analytical technique which depends upon the competition (affinity) of antigen for antigen-binding sites on antibody molecules. Although the foregoing radioimmunoassay techniques have proven to be valuable tools and have gained widespread acceptance, they are still not all that are to be desired because the antibody (immunoadsorbent) is consumed with each analysis hence must be discarded. xrint.com /patents/us/4108976   (5822 words)

Radioimmunoassay studies of human apolipoprotein E.   (Site not responding. Last check: 2007-10-08) Radioimmunoassay studies of human apolipoprotein E. Radioimmunoassay studies of human apolipoprotein E.Hyperlipidemic patients (n = 68) had higher mean apoE levels. The lipoprotein distribution of apoE was investigated by agarose column chromatography and ultracentrifugation of plasma. The plasma apoE level was independently related to plasma cholesterol and triglyceride levels in a population of 108 normal and nonchylomicronemic hyperlipidemic patients. www.pdg.cnb.uam.es /UniPub/iHOP/gp/3482801.html   (296 words)

Radioimmunoassay   (Site not responding. Last check: 2007-10-08) The technique was introduced in 1960 by Berson and Yalow as an assay for the concentration of insulin in plasma. Radioimmunoassay is widely-used because of its great sensitivity. The main drawbacks to radioimmunoassay are the expense and hazards if preparing and handling the radioactive antigen. users.rcn.com /jkimball.ma.ultranet/BiologyPages/R/Radioimmunoassay.html   (412 words)

Bupropion radioimmunoassay, and kit - Patent 4356165 The radioimmunoassay of the present invention is based on competition between bupropion and a fixed quantity of a distinguishable competitor of bupropion for a limited number of binding sites on bupropion-specific antibodies. Antisera to be used in the radioimmunoassay of the present invention must have high specificity for bupropion, and poor recognition of (i.e., low cross-reaction with) other species which might be present in the biological fluid to be tested, e.g., metabolites of bupropion. According to the preferred radioimmunoassay of the present invention, a standard curve is constructed by running the assay on two or more, preferably four to 8 solutions, each having a different known bupropion concentration within a suitable concentration range. www.freepatentsonline.com /4356165.html   (7342 words)

Determination of peplomycin in mouse tissues and biofluids by radioimmunoassay.   (Site not responding. Last check: 2007-10-08) Determination of peplomycin in mouse tissues and biofluids by radioimmunoassay. Peplomycin, an antitumour antibiotic analogue of bleomycin, was measured in mouse tissues using a rapid radioimmunoassay. We conclude that the radioimmunoassay is sensitive and reproducible and is an ideal tool for measuring peplomycin in tissues and biofluids for pharmacological studies. www.pdg.cnb.uam.es /UniPub/iHOP/gp/8048050.html   (151 words)

Radioimmunoassay of Creatine Kinase-B Isoenzyme in Human Sera: Results in Patients with Acute Myocardial Infarction -- ... Radioimmunoassay of Creatine Kinase-B Isoenzyme in Human Sera: Results in Patients with Acute Myocardial Infarction -- Willerson et al. A radioimmunoassay was developed to measure serum levels of the B isoenzyme of creatine kinase (ATP: creatine N-phosphotransferase, EC 2.7.3.2) (CPK) in order to evaluate the time course and frequency of MB isoenzyme elevation in patients with acute myocardial infarction. Thus, radioimmunoassay measurement of CPK-B isoenzyme appears to be a useful and sensitive test for the detection of acute myocardial infarcts in patients. www.pnas.org /cgi/content/abstract/74/4/1711   (361 words)

Radioimmunoassay agents - Patent 4013688 For example, such a position should be remote from the steroid ring A and D functionality of the sex hormone type of steroid, and from the combination of ring A and D functionality in adrenocortical hormones. In addition to their high specificity and sensitivity the novel compounds of this invention are far more available and easier to make than.sup.3 H or.sup.14 C-labeled steroids of the prior art and are suitable for use in practical, accurate, and economical laboratory procedures. The novel intermediates, useful in making the radioimmunoassay agents of this invention, have the following general structural formula: ##STR2## wherein St is a steroid moiety linked through the 11-carbon atom and corresponding to the steroid intended for assay and X is hydrogen or a cation such as an alkali metal ion or ammonia. freepatentsonline.com /4013688.html   (4007 words)

Effect of heparin on radioimmunoassay of gentamicin -- Schmelter et al. 38 (4): 534 -- American Journal of ... The effect of various heparin concentrations on the radioimmunoassay of gentamicin was studied in vitro and in rats. A radioimmunoassay for gentamicin was used in both the in vitro and in vivo tests. When 0-1000 units/ml of heparin were added to a constant amount of gentamicin (12 micro grams) in vitro, no difference was found in the assayed gentamicin concentration (p greater than 0.01). www.ajhp.org /cgi/content/abstract/38/4/534   (229 words)

The role of hypothalamic vasoactive intestinal polypeptide in the maintenance of prolactin secretion in incubating ... The role of chicken vasoactive intestinal polypeptide (cVIP) as a prolactin-releasing factor was investigated in incubating bantam hens. Specific antibodies were raised against cVIP (anti-cVIP) for passive immunization studies, to develop a radioimmunoassay and to localize VIP neurones immunohistochemically in the hypothalamus. The amount of cVIP, measured by radioimmunoassay, was significantly higher in the median eminence (P less than 0.01) and medial basal hypothalamus (P = 0.05) in incubating than in laying hens. joe.endocrinology-journals.org /cgi/content/abstract/122/1/5   (470 words)

Measurement of plasma thyroxine binding protein in relation to thyroidal condition in the turtle, Trachemys scripta, by ... The RIA demonstrated the presence of an immunochemically related protein in the plasma of several other species of Trachemys and in members of several other genera from the same family, Emydidae. The presence and level of radioimmunoassayable TBP in diverse species correlated with results of previous comparative measurements of T4 binding activity. Thus, in the turtle, this T4 transport protein may exist in higher concentrations and its levels are more variable and show a different relationship to thyroid activity than the analogous T4 binding globulin (TBG) in mammals. www.arclab.org /medlineupdates/abstract_1563614.html   (278 words)

Measurement of Plasma and Tissue Relaxin Concentrations in the Pregnant Hamster and Fetus Using a Homologous ... Measurement of Plasma and Tissue Relaxin Concentrations in the Pregnant Hamster and Fetus Using a Homologous Radioimmunoassay -- Renegar and Owens III 67 (2): 500 -- Biology of Reproduction Radioimmunoassay (RIA) of relaxin in sera of various species using an antiserum to porcine relaxin. Radioimmunoassay of relaxin in pregnancy with an analogue of human relaxin. www.biolreprod.org /cgi/content/full/67/2/500   (3931 words)

A radioimmunoassay for serum calcitonin in the rat -- Bell and Queener 152 (4): 516 -- Experimental Biology and Medicine A radioimmunoassay for serum calcitonin in the rat -- Bell and Queener 152 (4): 516 -- Experimental Biology and Medicine A radioimmunoassay for serum calcitonin in the rat A radioimmunoassay for serum calcitonin in the rat is described. www.ebmonline.org /cgi/content/abstract/152/4/516   (154 words)

Detection of the complement fragment C5a in inflammatory exudates from the rabbit peritoneal cavity using ... Detection of the complement fragment C5a in inflammatory exudates from the rabbit peritoneal cavity using radioimmunoassay -- Jose et al. Detection of the complement fragment C5a in inflammatory exudates from the rabbit peritoneal cavity using radioimmunoassay We describe a radioimmunoassay for rabbit C5a and its use to obtain www.jem.org /cgi/content/abstract/158/6/2177   (147 words)

Protocol for Metcombi Radioimmunoassay The METCOMBI Radioimmunoassay provides material for the quantitative measurement of derivatized normetanephrine and metanephrine in urine. The assay procedure follows the basic principle of radioimmunoassays: the competition between a radioactive and a non-radioactive antigen for a fixed number of antibody-binding sites. The amount of 125I-labeled antigen bound to the antibody is inversely proportional to the analyte concentration of the sample. www.kdra.or.kr /screen/FR01010.htm   (866 words)

O VAKKURI et al.: Ouabain radioimmunoassay (Journal of Endocrinology) Ouabain was recently isolated from human plasma, bovine hypothalamus and bovine adrenal in attempts to identify endogenous substances inhibiting the cell membrane sodium pump. A number of radioimmunoassays have been developed in order to study the clinical significance of ouabain. Plasma extracts and previously isolated human plasma ouabain-like compound and bovine hypothalamic inhibitory factor displaced the tracer parallelly and eluted identically with ouabain in high-performance liquid chromatography. journals.endocrinology.org /joe/165/joe1650669.htm   (341 words)

NEJM -- Radioimmunoassay of myelin basic protein in spinal fluid. An index of active demyelination NEJM -- Radioimmunoassay of myelin basic protein in spinal fluid. Radioimmunoassay of myelin basic protein in spinal fluid. Since this article has no abstract, we have provided an extract of the first words of the full text and any section headings. content.nejm.org /cgi/content/extract/295/26/1455   (132 words)

A comparison between the Farr radioimmunoassay and a new automated fluorescence immunoassay for the detection of ... A comparison between the Farr radioimmunoassay and a new automated fluorescence immunoassay for the detection of antibodies against double stranded DNA in serum -- Derksen et al. A comparison between the Farr radioimmunoassay and a new automated fluorescence immunoassay for the detection of antibodies against double stranded DNA in serum Conclusions: The Farr radioimmunoassay is superior to the ELIA ard.bmjjournals.com /cgi/content/full/61/12/1099   (2006 words)

Abebooks Search Results - Radioimmunoassay Principles of radioimmunoassay and their application in measuring renin and angiotensin. This book is designed as an introductory test for the staff of clinical research laboratories who conduct or intend to conduct such techniques, and will be of great value to the clinicians who make use of such services. The volume takes a three-pronged approach in it's in-depth presentation: explanation of the basic principles and applications of radioimmunoassays and non-isotopic immunoassays; practical illustrations of the various steps involved in immunoassays; discussion of the problems and pitfalls in immunoassays and how to avoid them. awww.abebooks.co.uk /search/sortby/3/kn/Radioimmunoassay   (1923 words)

Diabetes : Peptide-based radioimmunoassay for insulin receptor: detection of insulin-stimulated downregulation in IM-9 ...   (Site not responding. Last check: 2007-10-08) Start / D / Diabetes / August 01, 1989 / Peptide-based radioimmunoassay for insulin receptor: detection of insulin-stimulated downregulation in IM-9 lymphocytes. Peptide-based radioimmunoassay for insulin receptor: detection of insulin-stimulated downregulation in IM-9 lymphocytes. To overcome the difficulties encountered in quantifying the insulin receptor number by Scatchard analysis, a radioimmunoassay (RIA) for the human insulin receptor (hIR) has been developed that uses an antibody raised against a synthetic peptide (Gly-Lys-Lys-Asn-Gly-Arg-Ile-Leu-Thr-Leu-Pro-Arg-Ser-Asn-Pro-Ser) corresponding to the carboxyl terminal of the hIR. static.highbeam.com /d/diabetes/august011989/peptidebasedradioimmunoassayforinsulinreceptordete/index.html   (229 words)

Measurement by radioimmunoassay of casein content in rabbit mammary gland during pregnancy and after prolactin ... Measurement by radioimmunoassay of casein content in rabbit mammary gland during pregnancy and after prolactin stimulation in organ culture -- Jahn et al. Measurement by radioimmunoassay of casein content in rabbit mammary gland during pregnancy and after prolactin stimulation in organ culture A specific homologous radioimmunoassay was developed to measure rabbit www.ebmonline.org /cgi/content/abstract/184/1/19   (218 words)

BADERC Radioimmunoassay Core The Radioimmunoassay Core provides technical services and help for immunoassays and the development of immunoreagents. Additionally, the Core provides in-house and commercial radioimmunoassays that have been established for many years in Dr Habener’s laboratory for metabolic studies in animals and humans. Thus, the Radioimmunoassay Core also maintains a monoclonal antibody facility that can be accessed by Members of BADERC. www.baderc.org /cores/radioimmunCore.html   (237 words)

Radioimmunoassay for Intact Gross Mouse Leukemia Virus -- Yalow and Gross 73 (8): 2847 -- Proceedings of the National ... Radioimmunoassay for Intact Gross Mouse Leukemia Virus -- Yalow and Gross 73 (8): 2847 -- Proceedings of the National Academy of Sciences A radioimmunoassay for intact Gross leukemia virus has been developed using I-labeled Gross virus grown in tissue culture and guinea pig antisera to Gross virus grown either in tissue culture or harvested from leukemic C3H(f) mice. www.pnas.org /cgi/content/abstract/73/8/2847   (278 words)

Radioimmunoassay for detecting antibodies against murine malarial parasite antigens: monoclonal antibodies recognizing ...   (Site not responding. Last check: 2007-10-08) Radioimmunoassay for detecting antibodies against murine malarial parasite antigens: monoclonal antibodies recognizing Plasmodium yoelii antigens -- Kim et al. Radioimmunoassay for detecting antibodies against murine malarial parasite antigens: monoclonal antibodies recognizing Plasmodium yoelii antigens A solid-phase radioimmunoassay (SPRIA) in microtiter wells was established www.jimmunol.org /cgi/content/abstract/125/6/2565   (290 words)

Radioimmunoassay of serum glycocholic acid, standard laboratory tests of liver function and liver biopsy findings: ...   (Site not responding. Last check: 2007-10-08) Radioimmunoassay of serum glycocholic acid, standard laboratory tests of liver function and liver biopsy findings: comparative study of children with liver disease -- Matsui et al. Radioimmunoassay of serum glycocholic acid, standard laboratory tests of liver function and liver biopsy findings: comparative study of children with liver disease Serum glycocholic acid (SGC) was measured by radioimmunoassay in 277 jcp.bmjjournals.com /cgi/content/abstract/35/9/1011   (369 words)

Measurement of Brevetoxin Levels by Radioimmunoassay of Blood Collection Cards after Acute, Long-Term, and Low-Dose ... We developed a radioimmunoassay (RIA) using a sheep anti-brevetoxin antiserum to evaluate detection of brevetoxin on blood collection cards from mice treated with the brevetoxin congener PbTx-3. In this article, we describe a brevetoxin radioimmunoassay (RIA) that we developed using a format parallel to the RBA, and compare the methods for detection of brevetoxin in the blood of exposed animals. Radioimmunoassay for PbTx-2-type brevetoxins: epitope specificity of two anti-PbTx sera. ehp.niehs.nih.gov /members/2003/6166/6166.html   (5752 words)

Active site radioimmunoassay for human urokallikrein and demonstration by radioimmunoassay of a latent form of the ... Active site radioimmunoassay for human urokallikrein and demonstration by radioimmunoassay of a latent form of the enzyme -- Silver et al. Active site radioimmunoassay for human urokallikrein and demonstration by radioimmunoassay of a latent form of the enzyme radioimmunoassay for the 21 urine samples before and after trypsin www.jimmunol.org /cgi/content/abstract/124/4/1551   (319 words)

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