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Topic: RasGAP

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	Epitomics RasGAP (C-term) rabbit antibody - 1715-1
		RasGAP is a Caspase 3 substrate (1) and acts as both pro- and anti-apoptotic protein depending on the extent of its cleavage by caspases (2).
		At low levels of caspase activity, RasGAP is cleaved at position 455, generating an N-terminal fragment (fragment N) and a C-terminal fragment (fragment C).
		Fragment N appears to be a general blocker of apoptosis downstream of caspase activation because it inhibits caspase 9-induced cell death.
	www.epitomics.com /products/1715-1.php (263 words)

	Identification of Aurora Kinases as RasGAP Src Homology 3 Domain-binding Proteins -- Gigoux et al. 277 (26): 23742 -- ...
		melanogaster RasGAP was fused in-frame with the GAL4
		B, the immunoblot was probed with a mAb directed against RasGAP as a control for the expression and immunoprecipitation of RasGAP in untransfected and transfected COS cells.
		RasGAP Interacts with the Aurora Kinase Domain and Inhibits HsAIRK Activity-- Two truncated versions of Aurora were generated in an attempt to locate the region involved in the binding of Aurora to the
	www.jbc.org /cgi/content/full/277/26/23742 (3673 words)

	Cleavage of RasGAP and Phosphorylation of Mitogen-Activated Protein Kinase in the Course of Coxsackievirus B3 ...
		Cleavage of RasGAP and Phosphorylation of Mitogen-Activated Protein Kinase in the Course of Coxsackievirus B3 Replication -- Huber et al.
		RasGAP (120 kDa) and the 104-kDa protein are indicated.
		RasGAP and RasGAP-related proteins of 104 and 98 kDa are indicated.
	jvi.asm.org /cgi/content/full/73/5/3587 (5902 words)

	[No title]
		RASGAP will pursue a systematic decision-making process to determine which proposals will be awarded State Sea Grant funds.
		Policy: The RASGAP shall encourage and support research and programs that promote the understanding and development of environmentally sustainable uses of coastal and/or ocean resources that are important to California's economic well-being.
		Policy: The RASGAP shall encourage and support research and programs that help safeguard the public's health, safety and welfare relative to coastal and/or ocean uses and that protect or restore the quality of coastal and/or ocean waters which sustain the State's living marine resources.
	resources.ca.gov /ocean/html/appendixg.html (1105 words)

	Interactive Fly, Drosophila
		Ectopic expression of RasGAP in the wing imaginal disc reduces the size of the adult wing by up to 45% and suppresses ectopic wing vein formation caused by expression of activated forms of Breathless and Heartless, two Drosophila receptor tyrosine kinases of the fibroblast growth factor receptor family.
		Drosophila RasGAP appears to be not efficiently recruited to the plasma membrane by the Egfr RTK, and this is consistent with the failure to detect an effect of RasGAP overexpression on photoreceptor development in the eye, a process controlled by the Egfr and Sev RTKs, neither of which contains the RasGAP SH2 binding consensus.
		RasGAP attenuates FGFR signalling in vivo and this ability is dependent on both its SH2 domains and its GAP activity.
	www.sdbonline.org /fly/torstoll/ras85-4b.htm (5220 words)

	Antiapoptotic Signaling Generated by Caspase-Induced Cleavage of RasGAP -- Yang and Widmann 21 (16): 5346 -- Molecular ...
		In panel B, cell lysates corresponding to conditions leading to similar protein expression levels (as determined from panels C to G) were analyzed by Western blotting using an antibody that specifically recognizes the active form of caspase 3 or an antibody that recognizes the caspase-generated p85 fragment of PARP.
		The extent of RasGAP cleavage in HeLa cells was a function of the cisplatin concentration.
		RasGAP is a caspase substrate that is sequentially cleaved during apoptosis.
	mcb.asm.org /cgi/content/full/21/16/5346 (7150 words)

	Partial Cleavage of RasGAP by Caspases Is Required for Cell Survival in Mild Stress Conditions -- Yang et al. 24 (23): ...
		The cleavage of RasGAP and the measure of apoptosis was then analyzed as described for panel D. The absence of caspase 8 did not prevent the cleavage of RasGAP in cells undergoing apoptosis.
		MEFs stably expressing a plasmid encoding the uncleavable D455A RasGAP mutant were electroporated with the indicated combinations of plasmids encoding fragment N and Akt-DN (in the presence of a GFP-encoding plasmid) and were incubated for 24 h with the indicated compounds.
		RasGAP cleavage into fragment N occurred in the absence of apoptosis, while further processing of fragment N occurred when the cells underwent apoptosis and ICAD was cleaved.
	mcb.asm.org /cgi/content/full/24/23/10425 (6942 words)

	FBM UNIL - Prof. Christian Widmann (Site not responding. Last check: 2007-11-05)
		RasGAP is a regulator of Ras- and Rho-dependent pathways but also a caspase-3 substrate that bears two cleavage sites that are used sequentially as caspase activity increases.
		The survival of cells having mildly activated caspase-3, in response to low stresses for example is critically dependent on the partial cleavage of RasGAP into fragment N. The second cleavage of RasGAP results in the destruction of fragment N and its anti-apoptotic activity.
		RasGAP is the first example of a caspase substrate that, when cleaved, negatively or positively regulates apoptosis in a manner that is dependent on the extent of caspase activation.
	www.unil.ch /fbm/page28749_en.html (928 words)

	ICI/FOCIS 2004 Abstracts (Site not responding. Last check: 2007-11-05)
		With the use of a Raf-1-kinase pull-down assay, the defective MAP-kinase response in TSAd-deficient T cells was attributed to impaired switching of the small G-protein, Ras, from an inactive GDP-bound to an active GTP-bound form.
		Consistent with this, RasGAP activity remained at constitutively high levels in TSAd-deficient T cells after TCR stimulation although was turned-off in control T cells in response to the same stimulus.
		Thus, TSAd is identified as a novel inhibitor of RasGAP that is necessary for activation of the Ras-MAP-kinase pathway in T cells.
	www.immuno2004.org /onlineabstracts/941.html (382 words)

	Impaired Akt Activity Down-Modulation, Caspase-3 Activation, and Apoptosis in Cells Expressing a Caspase-resistant ...
		MEFs stably transfected with plasmids bearing the wild-type RasGAP cDNA or a cDNA encoding the D157A mutant of RasGAP were analyzed for their RasGAP expression levels by Western blot using an antibody directed at the SH domains of RasGAP.
		MEF clones stably expressing a wild-type RasGAP-encoding plasmid or a plasmid encoding a RasGAP mutant that cannot be cleaved at position 157 (D157A) were subjected to the indicated concentrations of cisplatin for 24 h.
		MEFs stably transfected with plasmids bearing the wild-type RasGAP cDNA or a cDNA encoding the D157A mutant of RasGAP were starved for 24 h in DMEM lacking serum and stimulated for 15 min with 15% NBCS.
	www.molbiolcell.org /cgi/content/full/16/8/3511 (5760 words)

	[No title]
		The RASGAP shall encourage its members to identify specific research projects on an annual basis (either funded or likely to be funded) that could potentially benefit from management by Sea Grant by gaining efficient access to top university researchers, well developed peer review procedures, and possible matching funds.
		B. Economically Important Coastal and Ocean Commercial Uses Policy: The RASGAP shall encourage and support research and programs that promote the understanding and development of environmentally sustainable uses of coastal and/or ocean resources that are important to California's economic well-being.
		C. Public and Environmental Health, Safety, and Welfare Policy: The RASGAP shall encourage and support research and programs that help safeguard the public's health, safety and welfare relative to coastal and/or ocean uses and that protect or restore the quality of coastal and/or ocean waters which sustain the State's living marine resources.
	www.usc.edu /org/seagrant/TextOnly/2005CFP/CalifPriorities.doc (1385 words)

	Role of rap1B and p21ras GTPase- activating protein in the regulation of phospholipase C-gamma 1 in human platelets.
		Using specific antibodies, we found that phospholipase C-gamma 1 and the p21ras GTPase-activating protein, rasGAP, are present in human platelets.
		Our results suggest the formation of a signaling complex involving rasGAP, phospholipase C-gamma 1, and rap1B that might be important in the cascade leading to platelet activation.
		Although thrombin stimulation had no effect on the level of phosphorylation of phospholipase C-gamma 1 and only slightly increased the tyrosine phosphorylation of rasGAP, the agonist induced the association of rasGAP with rap1B, as indicated by the appearance of rap1B on a Western blot of rasGAP immunoprecipitates.
	www.ihop-net.org /UniPub/iHOP/gp/67260.html (191 words)

	RasGTPase-activating protein is a target of caspases in spontaneous apoptosis of lung carcinoma cells and in response ...
		RasGAP was eluted by using 3 ml of 100 mM glycine (pH 2.5) and
		The cytosolic fraction contained RasGAP and fragment N2 but lacked fragment N.) The corresponding cleavage of PARP into fragment p85 was also analysed by western blot in total protein lysates.
		The presence of RasGAP and its cleavage fragments (fragments N, N2 and C) (upper two panels), and PARP and its cleavage fragment (bottom panel) were visualized by western blot.
	carcin.oxfordjournals.org /cgi/content/full/25/6/909 (6155 words)

	A protein kinase C-independent pathway leading to c-Jun-dependent expression of 100-kDa Ras GTPase-activating protein ... (Site not responding. Last check: 2007-11-05)
		RasGAP de novo synthesis to okadaic acid was not affected by
		RasGAP expression, which is independent of protein kinase C. In addition, okadaic acid treatment resulted in the activation
		RasGAP expression; and (b) that the activation of c-Jun is the
	content.febsjournal.org /cgi/content/full/267/6/1589 (6083 words)

	Role of pp60c-srcand p44/42 MAPK in ANG II-induced contraction of rat tonic gastrointestinal smooth muscles -- Puri et ...
		rasGAP antibodies on ANG II-induced contraction of SMC from LES and IAS are shown in C and D, respectively.
		rasGAP in the LES and IAS smooth muscles in the basal state and after ANG II.
		rasGAP were prepared by using mouse monoclonal anti-phosphotyrosine antibody (primary antibody, 1:1,000) and anti-mouse IgG-HRP (secondary antibody, 1:2,000).
	ajpgi.physiology.org /cgi/content/full/283/2/G390 (5483 words)

	Deregulation of the Egfr/Ras Signaling Pathway Induces Age-related Brain Degeneration in the Drosophila Mutant vap -- ...
		Several reports implicate RasGAP in controlling a cell death mechanism that could be dependent on deregulation of Ras.
		To investigate the physiological importance of RasGAP in the process of neuronal cell survival, we have isolated and characterized
		(B) RasGAP is expressed in the entire brain cortex, suggesting widespread expression in cortical cell bodies as detected by using an antisense RNA probe.
	www.molbiolcell.org /cgi/content/full/14/1/241 (6443 words)

	RasGAP IB1 LDL (Site not responding. Last check: 2007-11-05)
		1) Role of the cleavage of RasGAP by caspases during apoptosis.
		Cells expressing the D157A mutant of RasGAP display selective survival capacity.
		RasGAP–/– cells stably expressing plasmids encoding either wild-type RasGAP (second row), or the uncleavable D455A mutant (two independent clones; bottom rows) were plated in 10-cm dishes and incubated for 8 days in the absence (left lane) or in the presence (right lane) of 0.5 µM cisplatin.
	ibcmsg6.unil.ch /research/widmann/index.html (248 words)

	Interactive Fly, Drosophila
		Consequently, RasGAP undergoes a conformational change that results in a 100-fold increase in the accessibility of the target binding surface of its SH3 domain.
		The three-dimensional structure of the complex between human H-Ras bound to guanosine diphosphate and the guanosine triphosphatase (GTPase)-activating domain of the human GTPase-activating protein p120(GAP) in the presence of aluminum fluoride was solved at a resolution of 2.5 angstroms.
		Its sequence is 30% identical to p120 RasGAP and it contains the FLR PA P motif diagnostic for RasGAPs.
	www.sdbonline.org /fly/torstoll/ras85-2a.htm (4589 words)

	Epidermal growth factor increases protein and messenger RNA expression levels of Ras GTPase activating protein -- Soler ...
		The Ras GTPase activating protein (RasGAP) is a key regulatory enzyme in
		The mechanism for RasGAP protein induction by EGF
		up-regulation of rasGAP is a delayed response, since the increase in the
	cgd.aacrjournals.org /cgi/content/abstract/5/5/519 (319 words)

	Pfam 21.0 : RasGAP (Site not responding. Last check: 2007-11-05)
		As it is the GTP bound form of ras which is active, these proteins are said to be down-regulators of ras.
		The Ras GTPase-activating proteins are quite large (from 765 residues for sar1 to 3079 residues for IRA2) but share only a limited (about 250 residues) region of sequence similarity, referred to as the 'catalytic domain' or rasGAP domain.
		Note: There are distinctly different GAPs for the rap and rho/rac subfamilies of ras-like proteins (reviewed in reference PUBMED:8259209) that do not share sequence similarity with ras GAPs.
	pfam.janelia.org /cgi-bin/getdesc?name=RasGAP (205 words)

	Dok-1 tyrosine residues at 336 and 340 are essential for the negative regulation of Ras-Erk signalling, but dispensable ...
		Tyrosine residues responsible for the rasGAP binding are indicated by arrows (top).
		The anti-Dok-1 immunoprecipitates (IP) or whole cell lysates (WCL) were subjected to immunoblotting (IB) with antibodies to rasGAP, Dok-1, or phosphotyrosine (pTyr).
		Arrowheads indicate the positions of rasGAP, Dok-1, or Dok-1 mutants.
	www.genestocellsonline.org /cgi/content/full/9/6/601 (2917 words)

	Department of Cell Biology and Molecular Medicine Home Page
		Mainly, we discovered that it is involved in the enhancement of Cdk7 and RNA polymerase II activities in cardiac cells, a mechanism that leads to the increase in total RNA and protein in the cell, which is the hallmark of hypertrophy.
		Recent studies form our and others laboratories have recently identified a major role for Cdk7 and RNA polymerase II incell survival, thus, potentially linking the hypertrophy pathway to that of cell survival.
		Current work in the laboratory aims to further delineate the role of RasGAP in cell survival and its relationship to hypertrophy both in cultured cardiac myocytes and in transgenic and knockout mouse models.
	www.umdnj.edu /njmsweb/anatomy/cbmm_faculty_bio_mabdellatif.html (414 words)

	InterPro: IPR000593 RasGAP protein, C-terminal
		Ras GTPase-activating protein (rasGAP) is a major contributor to the downregulation of ras by facilitating GTP hydrolysis of activated ras.
		Abnormal signal transduction involving activated ras genes plays a major role in the development of a variety of tumors.
		Depending on the precise genetic alteration, its location within the gene and the effects it exerts on protein function, rasGAP can theoretically function as either an oncogene or as a tumor suppressor gene [ 1 ].
	www.ebi.ac.uk /interpro/IEntry?ac=IPR000593 (2887 words)

	BCL6 suppresses RhoA activity to alter macrophage morphology and motility -- Pixley et al. 118 (9): 1873 -- Journal of ...
		RhoGAP and RasGAP association and translocation to the plasma membrane are reduced in Bcl6
		RasGAP with the FMS oncoprotein, we were unable to demonstrate
		to the plasma membrane is mediated by RasGAP in macrophages,
	jcs.biologists.org /cgi/content/full/118/9/1873 (5965 words)

	Regulation of Extracellular Signal-regulated Kinase Activity by p120 RasGAP Does Not Involve Its Pleckstrin Homology or ...
		Our results could be explained by the activation of other rasGAPs.
		Kim J. H., Liao D., Lau L. F., Huganir R. SynGAP: a synaptic RasGAP that associates with the PSD-95/SAP90 protein family.
		Roof R. W., Haskell M. D., Dukes B. D., Sherman N., Kinter M., Parsons S. Phosphotyrosine (p-Tyr)-dependent, and -independent mechanisms of p190 RhoGAP-p120 RasGAP interaction: Tyr 1105 of p190, a substrate for c- Src, is the sole p-Tyr mediator of complex formation.
	cgd.aacrjournals.org /cgi/content/full/12/11/551 (6095 words)

	Phosphoinositide 3-Kinase-dependent Membrane Recruitment of p62dok Is Essential for Its Negative Effect on ...
		Nck, RasGAP, and Csk (1) (4) (8) (13) (15) (16) (17).
		Total protein extracts were immunoprecipitated with Abs against Myc and subjected to Western blot analysis using Abs against RasGAP and Nck (Transduction Laboratories).
		Songyang, Z, Yamanashi, Y., Liu, D., and Baltimore, D. Domain-dependent function of the rasGap binding protein p62Dok in cell signaling.
	www.jem.org /cgi/content/full/194/3/265 (5615 words)

	p62dok Negatively Regulates CD2 Signaling in Jurkat Cells -- Némorin et al. 166 (7): 4408 -- The Journal of ...
		Jurkat J.77-6 cells (A) or clone HA-1 cells (B) or clone 2 cells (C) were left unstimulated (-) or stimulated with an anti-CD2 mAbs pair for 3 min or anti-CD3 mAbs for 1 min.
		The band indicated by an asterisk corresponds to phosphorylated ZAP-70 associated with CD3 and is due to stimulating CD3 mAbs that bind to protein A during the immunoprecipitation.
		the relocalization of RasGAP is mediated by phosphorylated p62
	www.jimmunol.org /cgi/content/full/166/7/4408 (4832 words)

	Differential Effects of B Cell Receptor and B Cell Receptor-Fcgamma RIIB1 Engagement on Docking of Csk to ...
		The 62-kD protein bound to Csk is associated with RasGAP.
		One common feature of proteins that associate with p62, directly or indirectly, is their involvement in cytoskeletal rearrangements.
		Address correspondence to Dr. Milena Vuica, Department of Molecular Biology and Genetics, Howard Hughes Medical Institute, The Johns Hopkins University School of Medicine, PCTB 707, 725 N. Wolfe St., Baltimore, MD 21205.
	www.jem.org /cgi/content/full/186/2/259 (5326 words)

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