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Topic: RecQ helicase

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Helicase

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		The founding member of the family, RecQ of Escherichia coli, was identified in a study aimed at clarifying the mechanism of thymineless death, a phenomenon underlying the mechanism for the cytotoxicity of the anticancer drug 5-fluorouracil.
		Helicases are molecular motors that use the energy of nucleoside 5'-triphosphate (NTP) hydrolysis to translocate along a nucleic acid strand and catalyse reactions such as DNA unwinding.
		BACH1 helicase requires a minimal 5' ssDNA tail of 15 nucleotides for unwinding of conventional duplex DNA substrates; however, the enzyme is able to catalytically release the third strand of the homologous recombination intermediate D-loop structure irrespective of DNA tail status.
	www.pdg.cnb.uam.es /martink/LINKS/helicase.xml (19928 words)

	ABBS 2005,37(09): RecQ Helicase-catalyzed DNA Unwinding Detected by Fluorescence Resonance Energy Transfer
		The RecQ family helicases BLM and WRN are mutated in Bloom's syndrome and Werner's syndrome, respectively, and RecQ4 is defective in Rothmund-Thomson syndrome.
		RecQ protein was pre-incubated with fluorescent-labeled dsDNA substrate in unwinding buffer at 25 ºC, and the emission spectrum was measured.
		The dsDNA substrate and RecQ helicase were mixed and incubated at 25 ºC for 5 min in unwinding buffer A in syringe 1 and the unwinding reaction was initiated by rapid mixing with ATP from syringe 4.
	www.abbs.info /fulltxt/37-09/37090593.htm (3478 words)

	The Sekelsky Lab RecQ5 (Site not responding. Last check: 2007-09-17)
		Helicases play essential roles in virtually all DNA metabolic pathways.
		RecQ helicases are conserved between prokaryotes and eukaryotes, and a multi-gene family exists in metazoa.
		RecQ helicases are clearly important in cellular metabolism.
	sekelsky.bio.unc.edu /Research/RecQ.html (273 words)

	Substrate-specific inhibition of RecQ helicase -- Wu and Maizels 29 (8): 1765 -- Nucleic Acids Research
		assays of RecQ in the presence of single-stranded DNA (1).
		Unwinding by RecQ (10 nm) was assayed on
		32 Naegeli,H.P.M. and Friedberg,E.C. (1993) The DNA helicase activities of Rad3 protein of Saccharomyces cerevisiae and helicase II of Escherichia coli are differentially inhibited by covalent and noncovalent DNA modifications J.
	nar.oxfordjournals.org /cgi/content/full/29/8/1765 (4291 words)

	UvrA and UvrB suppress illegitimate recombination: Synergistic action with RecQ helicase -- Hanada et al. 97 (11): 5989 ...
		In a previous study, we showed that RecQ helicase suppresses illegitimate recombination, with or without UV irradiation (8).
		Overproduction of UvrA Suppresses the Hyperrecombination Phenotype of the recQ and uvrB Mutants.
		In addition, the helicase activity of the UvrAB complex may be involved in suppression of illegitimate recombination in a manner similar to that proposed for RecQ helicase by Hanada et al.
	www.pnas.org /cgi/content/full/97/11/5989 (3416 words)

	The DNA binding properties of the Escherichia coli RecQ helicase. (Site not responding. Last check: 2007-09-17)
		The RecQ helicase family is highly conserved from bacteria to men and plays a conserved role in the preservation of genome integrity.
		To determine the thermodynamic parameters for the interaction of Escherichia coli RecQ helicase with DNA, equilibrium binding studies have been performed using the thermodynamic rigorous fluorescence titration technique.
		Steady-state fluorescence anisotropy measurements of fluorescein-labeled oligonucleotides revealed that RecQ helicase bound to DNA with an apparent binding stoichiometry of 1 protein monomer/10 nucleotides.
	www.arclab.org /medlineupdates/abstract_14665634.html (222 words)

	Prokaryotic and eukaryotic DNA helicases: Essential molecular motor proteins for cellular machinery -- Tuteja and ... (Site not responding. Last check: 2007-09-17)
		RecQ is a recombination-specific DNA helicase from the SF2 family.
		Hickson, I.D. (2003) RecQ helicase: caretakers of the genome.
		Hehman, G.L. and Hauswirth, W.W. (1992) DNA helicase from mammalian mitochondria.
	content.febsjournal.org /cgi/content/full/271/10/1835 (6183 words)

	DNA helicases in recombination and repair: construction of a delta uvrD delta helD delta recQ mutant deficient in ...
		DNA helicases in recombination and repair: construction of a delta uvrD delta helD delta recQ mutant deficient in recombination and repair -- Mendonca et al.
		DNA helicases in recombination and repair: construction of a delta uvrD delta helD delta recQ mutant deficient in recombination and repair
		delta recQ deletion mutation was lethal to the cell in a recBC sbcB(C) delta D background.
	jb.asm.org /cgi/content/abstract/177/5/1326 (518 words)

	RMI1/NCE4, a suppressor of genome instability, encodes a member of the RecQ helicase/Topo III complex
		The major defects of cells with mutated recQ helicases are hyper-recombination and genomic instability (Hickson, 2003).
		These data support models in which RecQ helicase action produces a toxic DNA structure that is resolved by Top3 (Gangloff et al, 1994; Ira et al, 2003; Wu and Hickson, 2003).
		RecQ helicases are thought to play a role in normal DNA replication.
	www.nature.com /emboj/journal/v24/n11/full/7600684a.html (7209 words)

	Biochemical Characterization of the DNA Helicase Activity of the Escherichia coli RecQ Helicase -- Harmon and ...
		Biochemical Characterization of the DNA Helicase Activity of the Escherichia coli RecQ Helicase -- Harmon and Kowalczykowski 276 (1): 232 -- Journal of Biological Chemistry
		We demonstrate that RecQ helicase from Escherichia coli is a catalytic helicase whose activity depends on the concentration
		The MER3 DNA Helicase Catalyzes the Unwinding of Holliday Junctions
	www.jbc.org /cgi/content/abstract/276/1/232 (1033 words)

	2003 Aging and Women's Health Research Poster Day (Site not responding. Last check: 2007-09-17)
		Evidence suggests that topoisomerases in association with the RecQ helicase family are essential in nucleic acid metabolism and the maintenance of genomic stability.
		We hypothesize that Escherichia coli topoisomerases and DNA helicases, similar to what has been proposed in eukaryotes, are capable of interacting to form a "toposome" that could act as an unwinding machine to play a role in DNA replication, recombination and repair.
		The RecQ helicase family is highly conserved from bacteria to humans.
	medschool.umaryland.edu /womenshealth/whrg/pikle08.html (458 words)

	Eurekah - DNA Repair Aspects For Recq Helicase Disorders
		RecQ family DNA helicases are defined by amino acid sequence similarities to Escheri chia coli RecQ which has been known to act in homologous recombination and to suppress illegitimate recombination, particularly during the repair of DNA double strand breaks.
		These data suggest that each of these helicases contributes to maintaining genomic stability and that an important function of these helicases appears to be the resolution of recombination intermediates.
		RecQ family DNA helicases are defined by amino acid sequence similarities to Escheri chia coli RecQ which has been known to act in homologous recombination and to suppress illegitimate recombination...
	www.eurekah.com /chapter/1955 (612 words)

	The archaeal Hjm helicase has recQ-like functions, and may be involved in repair of stalled replication fork -- ...
		recQ strain shows normal growth at 37.5 °C. coli mutant cells carrying the genes episomally, as indicated on the left side of each panel, were serially diluted and spotted on to plates.
		Bachrati, C.Z. and Hickson, I.D. (2003) RecQ helicases: suppressors of tumorigenesis and premature aging.
		Nakayama, K., Irino, N. and Nakayama, H. (1985) The recQ gene of Escherichia coli K12: molecular cloning and isolation of insertion mutants.
	www.genestocellsonline.org /cgi/content/full/11/2/99 (5170 words)

	Drosophila melanogaster RECQ5/QE DNA helicase: stimulation by GTP binding -- Kawasaki et al. 30 (17): 3682 -- Nucleic ...
		Helicase assays were performed using the substrate for determining the polarity of unwinding, as described in the Materials and Methods, using a 3'-labeled substrate (lanes 1–3, top) or 5'-labeled substrates (lanes 4–6, bottom) with RECQ5/QE protein (lanes 3 and 6, 0.8 pmol).
		Helicase assays were performed using a 20mer annealed to M13mp18 single-stranded circular DNA as the helicase substrate.
		Harmon,F.G. and Kowalczykowski,S.C. (2001) Biochemical characterization of the DNA helicase activity of the Escherichia coli RecQ helicase.
	nar.oxfordjournals.org /cgi/content/full/30/17/3682 (5491 words)

	Junction of RecQ Helicase Biochemistry and Human Disease -- Opresko et al. 279 (18): 18099 -- Journal of Biological ...
		RecQ helicases are a family of conserved enzymes required for
		intermediates arise and persist in the absence of RecQ helicases.
		Roles for RecQ helicases may not be limited to S-phase and HR related pathways.
	www.jbc.org /cgi/content/full/279/18/18099 (4004 words)

	2002 Aging and Women's Health Research Poster Day (Site not responding. Last check: 2007-09-17)
		The RecQ helicase family is highly conserved from bacteria to humans with homologs like E.
		The RecQ family helicases, in association with cellular topoisomerases, are required to maintain genomic integrity.
		In vitro, E. coli TopoIII, in conjunction with RecQ helicase, is capable of decatenating double-stranded interlinked DNA molecules and is proposed to play a role in chromosomal segregation.
	medschool.umaryland.edu /womenshealth/whrg/pikle36.html (347 words)

	The Aspergillus nidulans musN Gene Encodes a RecQ Helicase That Interacts With the PI-3K-Related Kinase UVSB -- Hofmann ...
		The Aspergillus nidulans musN Gene Encodes a RecQ Helicase That Interacts With the PI-3K-Related Kinase UVSB -- Hofmann and Harris 159 (4): 1595 -- Genetics
		The Aspergillus nidulans musN Gene Encodes a RecQ Helicase That Interacts With the PI-3K-Related Kinase UVSB
		RecQ helicases may be a common feature of the fungal proteome,
	www.genetics.org /cgi/content/full/159/4/1595 (4898 words)

	Premature Aging and Predisposition to Cancers Caused by Mutations in RecQ Family Helicases -- Furuichi 928 (1): 121 -- ...
		Premature Aging and Predisposition to Cancers Caused by Mutations in RecQ Family Helicases -- Furuichi 928 (1): 121 -- Annals of the New York Academy of Sciences
		helicase family also share sequence homologies between motifs.
		The Zinc Finger Motif of Escherichia coli RecQ Is Implicated in Both DNA Binding and Protein Folding
	www.annalsnyas.org /cgi/content/abstract/928/1/121 (411 words)

	InterPro: IPR002121 HRDC (Site not responding. Last check: 2007-09-17)
		The HRDC (Helicase and RNase D C-terminal) domain has a putative role in nucleic acid binding.
		Mutations in the HRDC domain associated with the human BLM gene result in Bloom Syndrome (BS), an autosomal recessive disorder characterized by proportionate pre- and postnatal growth deficiency; sun-sensitive, telangiectatic, hypo- and hyperpigmented skin; predisposition to malignancy; and chromosomal instability [ 1 ].
		Liu Z. Macias M.J. Bottomley M.J. Stier G. Linge J.P. Nilges M. Bork P. Sattler M. The three-dimensional structure of the HRDC domain and implications for the Werner and Bloom syndrome proteins.
	www.ebi.ac.uk /interpro/IEntry?ac=IPR002121 (2936 words)

	Structure and Function of RecQ DNA Helicases -- Bennett and Keck 39 (2): 79 -- Critical Reviews in Biochemistry and ...
		Articles by Bennett, R. Articles by Keck, J. Structure and Function of RecQ DNA Helicases
		RecQ family helicases play important roles in coordinating genome
		RecQ proteins, cells exhibit a variety of phenotypes, including
	www.crbmb.com /cgi/content/abstract/39/2/79 (430 words)

	Role of the Escherichia coli RecQ DNA helicase in SOS signaling and genome stabilization at stalled replication forks ...
		Role of the Escherichia coli RecQ DNA helicase in SOS signaling and genome stabilization at stalled replication forks -- Hishida et al.
		The RecQ protein family is a highly conserved group of DNA helicases
		In vitro, RecQ binds and unwinds forked DNA substrates
	www.genesdev.org /cgi/content/abstract/18/15/1886 (726 words)

	Sekelsky Lab RecQ Family (Site not responding. Last check: 2007-09-17)
		This dendrogram depicts the relationships between the sequences of the helicase regions of representative members of the eukaryotic RecQ helicase family (plus E.
		WRN (mutated in Werner syndrome) is found in humans and C.
		BLM (mutated in Bloom syndrome) is found in humans, C.
	sekelsky.bio.unc.edu /Research/RecQ_family.html (125 words)

	InterPro: IPR011545 DEAD/DEAH box helicase, N-terminal (Site not responding. Last check: 2007-09-17)
		The DEAD box helicases are involved in various aspects of RNA metabolism, including nuclear transcription, pre mRNA splicing, ribosome biogenesis, nucleocytoplasmic transport, translation, RNA decay and organellar gene expression.
		O01836 ATP-dependent RNA helicase glh-3 (EC 3.6.1.-) (Germline helicase 3)
		Bernstein D.A. Zittel M.C. Keck J.L. High-resolution structure of the E.coli RecQ helicase catalytic core.
	www.ebi.ac.uk /interpro/IEntry?ac=IPR011545 (2985 words)

	Human protein: P54132 - Bloom syndrome protein (EC -) (RecQ protein-like 3) (DNA helicase, RecQ-like type 2). EMBL ...
		The Bloom syndrome gene product is related to the RecQ subset of DExH box-containing DNA helicases and has both DNA-stimulated ATPase and ATP-dependent DNA helicase activities.
		Mutations causing Bloom syndrome delete or alter helicase motifs and may disable the 3'-5' helicase activity.
		The BLM helicase is necessary for normal DNA double-strand break repair.
	harvester.embl.de /harvester/P541/P54132.htm (1180 words)

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