| | FHA Group - In vitro Protein Recombination |
 | | Methods of laboratory recombination that are annealing-based (Stemmer, 1994; Zhao et al., 1998) or that are sequence-independent and random, (Ostermeier et al., 1999; Sieber et al., 2001) generate libraries of chimeras with unknown sequences and often limited diversity. |
 | | Contacts that cannot be broken by recombination events, those where one or both of the interacting amino acids are conserved in the two parents, are removed from the contact matrix. |
 | | Using site-directed recombination guided by SCHEMA we are now creating artificial protein families of cytochromes P450 to explore determinants of structure and function free from many of the constraints of natural selection. |
| www.che.caltech.edu /groups/fha/recombination.html (1836 words) |