| | Lecture 7, redox potentiometry (Site not responding. Last check: ) |
 | | In order to find the redox potential(s) of components in an uncharacterized system (for example, the cell membrane from a respiratory or photosynthetic bacterium which carries the electron transfer chain), the material is suspended in a stirred redox cuvette, which is placed in a spectrophotometer. |
 | | The process is repeated over the redox range of interest, and a set of data obtained representing a redox titration of absorbance change as a function of ambient redox potential. |
 | | Since the oxidized and reduced forms of the redox centers have different absorbance spectra, suitable choice of wavelength allows the absorbance change to be interpreted as a change in concentration of oxidized and reduced forms. |
| www.life.uiuc.edu /crofts/bioph354/redox_pot.html (597 words) |