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Topic: Replica plating

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	Replica plating - Wikipedia, the free encyclopedia
		The purpose of replica plating is to be able to compare the master plate and any secondary plates to screen for a selectable phenotype.
		Replica plating is especially useful for negative selection.
		By increasing the variety of secondary plates with different selective growth media, it is possible to rapidly screen a large number of individual isolated colonies for as many phenotypes as there are secondary plates.
	en.wikipedia.org /wiki/Replica_plating (279 words)

	Replica plating (Site not responding. Last check: 2007-11-04)
		Replica plating is a technique in which multiple dishes, also known as Petri plates, containing solid (agar-based) microbial media, are inoculated with between thirty and three-hundred colonies of microorganisms from a primary plate (or master dish), reproducing the original spatial pattern of colonies.
		The technique involves pressing a velvet-covered disk to a primary plate, and then imprinting multiple secondary plates with cells in colonies removed by the velvet from the original plate.
		By using different types of selective growth media (lacking nutrients or containing chemical growth inhibitors such as antibiotics) on the different secondary plates, it is possible to rapidly screen a large number of individual isolated colonies and detect those that have different phenotypes.
	www.encyclopedia-1.com /r/re/replica_plating.html (128 words)

	Replica plating (Site not responding. Last check: 2007-11-04)
		Replica plating is a technique in which multiple dishes, also known as Petri plate s, containing solid (agar -based) microbial media, are inoculated with between thirty and three-hundred colonies of microorganism s from a primary plate (or master dish), reproducing the original spatial pattern of colonies.
		The technique involves pressing a velvet -covered disk to a primary plate, and then imprinting multiple secondary plates with cells in colonies removed by the velvet from the original plate.
		By using different types of selective growth media (lacking nutrients or containing chemical growth inhibitors such as antibiotic s) on the different secondary plates, it is possible to rapidly screen a large number of individual isolated colonies and detect those that have different phenotypes.
	www.serebella.com /encyclopedia/article-Replica_plating.html (430 words)

	Patent 4634676: Replica plating device
		Since the replica plating provides for the transfer of colonies in the same pattern that the original colonies were incubated, ready identification of the source of cells of interest is the advantageous by-product of this technique.
		This replica plating device is comprised of a substantially planar pressing member, a side wall extending upwardly from the pressing member and surrounding same, and a skirt connected to the side wall and extending downwardly along the side wall in spaced relation thereto.
		Due to the structure of the replica plating device which includes a skirt therearound that embraces and covers the rim of the petri dish containing the cells for cloning, preservation of sterility in the petri dish is more assured than use of the presently known and available replica plating tool.
	www.freepatentsonline.com /4634676.html (3296 words)

	Replica plating coprinus
		Replica plating of Coprinus cinereus colonies using asexual spores
		Oidia were evidently transferred to the YMG/T replica plates from all colonies on the masterplate.
		Replica plating therefore clearly distinguished between the amounts of oidia produced by the different colonies on the master plate.
	www.fgsc.net /fgn44/polak.html (1076 words)

	Replica plating (Site not responding. Last check: 2007-11-04)
		Replica plating is a technique in which multiple dishes, also known as Petri plates, containing solid (agar -based) microbial media, are inoculated with betweenthirty and three-hundred colonies of microorganisms from a primary plate(or master dish), reproducing the original spatial pattern of colonies.
		The technique involves pressing a velvet -covered disk to a primary plate, and then imprinting multiple secondary plates with cells in coloniesremoved by the velvet from the original plate.
		By using different types of selective growthmedia (lacking nutrients or containing chemical growth inhibitors such as antibiotics) on the different secondary plates, it is possible to rapidly screen a large number of individualisolated colonies and detect those that have different phenotypes.
	www.therfcc.org /replica-plating-15135.html (129 words)

	Patent 5587322: Replica plating device
		The process of replica plating typically involves a printing-like transfer of a set of colonies from one culture surface to another culture surface or surfaces while maintaining the original pattern of the colonies.
		It is desirable in the replica plating process to use a device which is easy to operate and provides a large number of high resolution replicates.
		For example, the replica plating device may be composed of only two regions, a first for insertion into a petri dish, and the second being the peripheral ridge.
	www.freepatentsonline.com /5587322.html (4741 words)

	Replica Plating (Site not responding. Last check: 2007-11-04)
		The velvet is then pressed onto a plate with phage spread on the surface.
		When Lederberg pressed the replica pad with bacteria onto multiple plates containing bacteriophage, the number and position of resistant colonies was always the same.
		(We still use replica plating in the lab to check characteristics of 50- 100 colonies or 'patches' of bacteria - what nutrients are required for growth, antibiotic resistance, phage resistance, etc. (It's low tech and cheap but very useful!!).
	home.earthlink.net /~dayvdanls/replica_plating.htm (338 words)

	Dictionary of Meaning www.mauspfeil.net
		Generally, large numbers of colonies (roughly 30-300) are replica plated due to the difficulty in streak (biology) streaking each out individually onto a separate plate.
		The purpose of replica plating is to be able to compare the master plate and any secondary plates to genetic screen screen for a genetic marker selectable phenotype.
		By increasing the variety of secondary plates with different selective medium selective growth media, it is possible to rapidly screen a large number of individual isolated colonies for as many phenotypes as there are secondary plates.
	www.mauspfeil.net /Replica_plating.html (310 words)

	CollegeTermPapers - Science - Analyzing the origin of adaptive mutations in Saccharomyces cerevisiae - Free Term ...
		Of the three plates that were analyzed, the primary control plate possessed all of the colonies found on the original plate.
		When the three plates were incubated and consequently refrigerated to prevent further growth, observations were able to be made concerning the origin of adaptive mutants.
		The cells of the yeast culture that was produced on the master plate were replica plated onto a non-selective medium then transferred to the two copper-based plates.
	www.collegetermpapers.com /TermPapers/Science/Analyzing_the_origin_of_adaptive_mutations_in_Saccharomyces_cerevisiae.shtml (1675 words)

	Untitled Document (Site not responding. Last check: 2007-11-04)
		Replica plating is a process by which colonies on one plate are transferred with the same spatial arrangement to another plate.
		This is usually done by using a felt cloth and a cylindrical piece of metal with the same diameter as a plate.
		The plate is pressed onto the cylindrical metal covered by cloth, imprinting the bacteria onto the cloth.
	www.public.asu.edu /~laserweb/woodbury/classes/chm467/mating/replica.htm (68 words)

	February 07: Microbial genetics: DNA transfer, gene mapping.
		Individuals are replica plated on media having the nutrient (permissive media) vs. media lacking the nutrient (selective media).
		Replica plating (Figure 7.2) is commonly used for this purpose.
		Usually, one plate is "permissive," allowing the growth of all cells; the other is "restrictive," not allowing the growth of the mutants of interest.
	www.biosci.ohio-state.edu /~mgonzalez/Micro521/14.html (1145 words)

	[No title] (Site not responding. Last check: 2007-11-04)
		For replicating large numbers of master plates it is most convenient to remove the binder, wrap the wipers in aluminum foil, and autoclave them.
		The sheet is placed with the inner side up on a standard replica plating block and is secured with the retaining ring.
		A master plate is pressed firmly onto the surface and is lifted from one side to "peel" it from the replicating surface.
	www.fgsc.net /fgn/oneliner.html (486 words)

	Replica plating - Genetics Terms and Definitions by Health Dictionary
		Replica plating - Genetics Terms and Definitions by Health Dictionary
		A technique to rapidly transfer microorganism colonies from a master plate, in an exact spatial pattern, to a number of further plates.
		Information and definitions of the terms been taken from various reliable government publications and we have done our best to verify their accuracy.
	www.sciencedictionary.org /genetics-term-details/Replica-plating (78 words)

	REPLICA-PLATING TOOL (Site not responding. Last check: 2007-11-04)
		The Scienceware® Replica Plating Device utilizes a locking ring which secures sterile velveteen squares onto a PVC cylinder for easy control of the plating device.
		To produce replicas, the petri dish carrying microbial colonies is inverted and lowered onto the cloth surface and then lifted.
		Then a fresh sterile nutrient plate is lowered onto the velveteen surface and lifted, leaving a replica of the original colonies in the new dish.The replica plating device may be disinfected between uses with a brief rinse in ethanol or chlorine bleach.
	www.bestlabdeals.com /REPLICA_PLATING_TOOL_p/bel1629.htm (159 words)

	Vintage Timex Watch Forum, Information and History.
		I have never tried plating on plastic or using the graphite mixed with wax mold trick to use plating to form small objects.
		The result would be that an EXACT replica of the outside surface of the dial could be made in the mold.
		Of course, this replica dial would only have outside surface structural features on it, no printed markings, and be of only one color.
	www.network54.com /Forum/message?forumid=102266&messageid=1110823932 (302 words)

	SECTION VII
		Basically, the argument relied on an analysis of the variance of the plating results: whether the variance (i.e the "spread" on the different plates was narrow and close to the mean of all the plates, or was much greater than the mean.
		These invisible colonies on half the plates are then respread (to scatter out the progeny of the initial divisions so that they become new foci for colony growth) and a suspension of phage is sprayed evenly over the plate; these are compared to plates left undisturbed and sprayed with phage.
		Replica plate each onto 3 LB agar plates containing 10µg/ml streptomycin.
	www.waksmanfoundation.org /labs/wisteb/mutation.htm (4615 words)

	PlantMedia.com - Transformation, regeneration, and propagation of plants using ready media to ensure high efficiency in ...
		Microbe replica platers for efficient and rapid transfer of bacterial and yeast cultures to a fresh plate.
		Reusable velvet or velveteen squares are used to cover the upper surface of the replica plater and act as the substrate for accurate transfers.
		Solid metal ring with lock nut slips around the circumference of the plate contact surface and holds valvet squares firmly in place during use.
	www.plantmedia.com /MoreInfo.asp?ItemCode=270500 (86 words)

	Untitled Document (Site not responding. Last check: 2007-11-04)
		The master plate would not have a small number of resolved macrocolonies but a large number of unresolved microcolonies, among which there would be only a small number of resistant clones.
		My final comment, unrelated to the foregoing, is that the replica plating example demonstrates another major theme of the chapter; namely that any hypothesis is continually at risk of falsification by subsequent testing.
		I wish to emphasize that, although the pre-adaptation vs. directed mutation controversy had been considered settled for decades, and even though the fluctuation and replica plating tests were considered classics by the canonical texts, that bacterial geneticists were, for the most part, willing to accept that the question needed re-examination.
	bio.classes.ucsc.edu /bio1/webnotes/plating.html (834 words)

	Live Coverage of 2004 Pro Tour Seattle
		Jeroen played a Talisman, which gave him double fl to move the Plating at instant speed and attacked with his Alpha Myr and Gold Myr.
		Echoing Truth bounced the Plating and the Condor was consumed by the Replica.
		Jeroen replayed the Plating but there was a growing supply of tokens to clog up the ground, and with his Condor gone he could not fight his way through.
	www.wizards.com /default.asp?x=mtgevent/ptsea04/fin1 (1118 words)

	Replica plating (replica plate) (Site not responding. Last check: 2007-11-04)
		The microorganisms are cultured on a plate of agar, then a replica is made by blotting the colonies with a membrane and blotting the membrane on another plate.
		The second plate either contains some substance which kills off the mutation of interest, or lacks a substance necessary for the mutant to grow.
		Later, it is determined which colonies on the first plate failed to grow on the second plate.
	www.cartage.org.lb /en/themes/Reference/dictionary/Biologie/R/101.html (108 words)

	ABLE: Molecular Genetic Analysis in Yeast
		Replica plating from these complete medium plates onto leucine dropout plates will identify those colonies that are leucine auxotrophs and presumable have lost the LEU2 gene by random segregation.
		Replica plate each strain onto a master UEPD plate and a Leu dropout plate.
		An inexpensive replica plating block can be constructed by gluing a circular disk (8-cm diameter, 1-cm thick) onto the end of a hollow tube (8-cm long with an 8-cm outer diameter).
	www.zoo.utoronto.ca /able/volumes/vol-11/6-burke/6-burke.htm (7269 words)

	Genetics answers (Site not responding. Last check: 2007-11-04)
		replica plating from a medium without histidine to a medium with histidine
		After 30 minutes incubation the mixture was spread at various dilutions onto plates of minimal medium (containing glucose as the only organic substance).
		After being mixed they are plated on the media indicated.
	www.bact.wisc.edu /Bact303/geneticsanswers (1370 words)

	Replica1 (Site not responding. Last check: 2007-11-04)
		A sterile velvet pad is used to transfer colonies from a non-selective plate, on which both wild-type and mutant bacteria can grow, to a selective plate, on which only one or the other is able to grow.
		Typically, this technique is used to identify auxotrophic mutants by transferring them from a nutrient-rich plate to minimal medium on which only prototrophs can grow.
		Once identified by their failure to grow on the minimal plates, such auxotrophic mutants can be recovered from the nutrient rich master plate.
	www.bi.umist.ac.uk /users/mjfssps/1GAG/Replica1.htm (124 words)

	Learn more about Replica plating in the online encyclopedia. (Site not responding. Last check: 2007-11-04)
		Learn more about Replica plating in the online encyclopedia.
		Hint: Play with putting spaces before and after your words to see the different results you get.
		The technqiue involves pressing a velvet covered disk to a primary plate, and then imprinting multiple secondary plates with cells in colonies removed by the velvet from the original plate.
	www.onlineencyclopedia.org /r/re/replica_plating.html (217 words)

	Screening for antimicrobial resistance in fecal samples by the replica plating method -- Osterblad et al. 33 (12): 3146 ...
		Screening for antimicrobial resistance in fecal samples by the replica plating method -- Osterblad et al.
		Replica plating can be used for the detection of antibiotic resistance in
		replica plates in the study gave results similar to those produced by the
	jcm.asm.org /cgi/content/abstract/33/12/3146 (363 words)

	28361. Gold plating large non-conductive substrates [Florida]
		We are in the need of a business that can gold plate non-conductive material - Fiberglass / gelcoat.
		Currently we are fabricating a 20' tall replica of the World Series trophy and need some rather large parts gold plated.
		Basically a tank that can hold a 3' x 3' x 12' block to be able to do all the parts in their smallest break-down form.
	www.finishing.com /283/61.shtml (105 words)

	Dictionary definition of REPLICA PLATING (Site not responding. Last check: 2007-11-04)
		A sterile velvet pad, the same size as the petri dish, is then pressed onto it, picking up a sample of each colony.
		The bacteria can then be stamped onto new sterile petri dishes, plates, in the identical arrangement.
		The media in the new plates can be made up to lack specific nutritional requirements or to contain antibiotics.
	www.dictionarybarn.com /REPLICA-PLATING.php (219 words)

	Replica Plating and in situ Enzymatic Assay of Animal Cell Colonies Established on Filter Paper -- Esko and Raetz 75 ...
		Replica Plating and in situ Enzymatic Assay of Animal Cell Colonies Established on Filter Paper -- Esko and Raetz 75 (3): 1190 -- Proceedings of the National Academy of Sciences
		We have developed a simple technique for the replica plating of Chinese hamster ovary (CHO) cells.
		The cell colonies transferred to the paper are viable and can be replica plated to a new petri dish with high resolution.
	www.pnas.org /cgi/content/abstract/75/3/1190 (341 words)

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