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Topic: Restriction fragment length polymorphism

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	RFLP Definition (Site not responding. Last check: 2007-11-02)
		Restriction Fragment Length Polymorphism (RFLP) is a technique in which organisms may be differentiated by analysis of patterns derived from cleavage of their DNA.
		Restriction enzymes are isolated from a wide variety of bacterial genera and are thought to be part of the cell's defenses against invading bacterial viruses.
		Isolation of sufficient DNA for RFLP analysis is time-consuming and labor intensive.
	vm.cfsan.fda.gov /~frf/rflp.html (300 words)

	Restriction fragment length polymorphism
		Restriction fragment length polymorphism (or RFLP) is a property of DNA used in molecular biology methods, for example, genetic fingerprinting or paternity testing.
		The restriction fragments are then separated according to length by agarose gel electrophoresis.
		The distance between the locations cut by restriction enzymes (the restriction sites) varies between individuals: so the length of the fragments varies, and the position of certain gel bands differs between individuals (thus polymorphism).
	www.ebroadcast.com.au /lookup/encyclopedia/rf/RFLP.html (147 words)

	Restriction Fragment Length Polymorphism
		Restriction fragment length polymorphism is the identification of specific restriction enzymes that reveal a pattern difference between the DNA fragment sizes in individual organisms.
		Sample DNA is cut with one or more RE’s and resulting fragments are separated according to molecular size using gel electrophoresis.
		RFLP is most suited to studies at the intraspecific level or among closely related taxa.
	www.iscid.org /encyclopedia/Restriction_Fragment_Length_Polymorphism (222 words)

	RFLP's (Site not responding. Last check: 2007-11-02)
		Polymorphisms in the lengths of particular restriction fragments can be used as molecular markers on the genetic chromosome.
		The blot was probed by hybridization with a cloned fragment of the tree's genome.
		The length of the restriction fragments recognized by the probe is dimorphic in this population.
	opbs.okstate.edu /~melcher/MG/MGW1/MG11124.html (249 words)

	Title of Invention: Method and device for improved restriction fragment length polymorphism analysis
		Generally, sample DNA restriction fragments are separated on an agarose gel, denatured in the gel, transferred to a membrane (e.g., nylon) hybridized with labelled probe DNA, washed to remove unbound probe and finally evaluated through, for example, autoradiography.
		RFLPs occur as a result of base pair or positional changes in the restriction enzyme recognition sites which flank a chromosomal location and can be detected by hybridization of labelled DNA clones containing sequences that are homologous to a portion of the chromosomal fragment.
		The test plant genomic fragments which exhibit polymorphic characteristics however are frequently over 1 kb in their entirety, as the polymorphisms themselves may lie beyond the termini of the approximately 1 Kb RFLP probes we have used.
	www.nal.usda.gov /bic/Biotech_Patents/1994patents/05324631.html (7477 words)

	Restriction Fragment Lenght Polymorphism (RFLP) (Site not responding. Last check: 2007-11-02)
		Ethidium bromide staining is used to reveal the fragments under UV (260 nm) light.
		Restriction fragment length polymorphism (RFLP) is most suited to studies at the intraspecific level or among closely related taxa.
		Presence and absence of fragments resulting from changes in recognition sites are used identifying species or populations.
	nematode.unl.edu /its_id/pcr_rflp.htm (132 words)

	Analysis of LMP and TAP polymorphisms by polymerase chain reaction-restriction fragment length polymorphism in ...
		The bi-allelic polymorphism at codon 60 (Arg-His) in the LMP2 gene was analysed by polymerase chain reaction-restriction fragment
		Polymorphism at the TNF loci in rheumatoid arthritis.
		Characterization and polymorphism of the mouse MHC-linked Lmp-2 and Lmp-7 genes.
	ard.bmjjournals.com /cgi/content/full/57/1/33 (2652 words)

	Molecular Tools of Medicine
		Fragments in the size range of 15 - 25 kilobase pairs (kbp) are purified by gel electrophoresis or gradient centrifugation and ligated into a lambda vector.
		RFLP analysis takes advantage of this and utilizes Southern blotting of restriction enzyme digested genomic DNA to detect familial patterns of the fragments of a given gene, detectable by screening the Southern blot with a probe corresponding to the gene of interest.
		RFLP patterns are inherited and segregate in Mendelian fashion thus, allowing their use in genotyping such as in cases of paternity dispute or in criminal investigations.
	web.indstate.edu /thcme/mwking/molecular-medicine.html (4787 words)

	Restriction Fragment Length Polymorphisms (RFLPs)
		Polymorphisms are inherited differences found among the individuals in a population.
		If a particular RFLP is usually associated with a particular genetic disease, then the presence or absence of that RFLP can be used to counsel people about their risk of developing or transmitting the disease.
		Thus a large variety of alleles (polymorphisms) may be present in the population.
	home.comcast.net /~john.kimball1/BiologyPages/R/RFLPs.html (1598 words)

	DNA Fingerprinting (Site not responding. Last check: 2007-11-02)
		Restriction fragment length polymorphisms (RFLPs) is the term scientists use to denote that, when DNA is cut by restriction enzymes fragments of DNA are produced.
		The RFLP technique is considered more accurate than the PCR method, because it requires variations of the gene sequence that are found on longer strands.
		In other words, RFLP cannot be used in situations where very small strands of DNA are present, such as a crime scene, where a blood stain or a small piece of hair are the only evidence discovered.
	csdb.nidcr.nih.gov /csdb/chanchai_site/educ180/nih/DNA_how3.html (131 words)

	Restriction fragment length polymorphism analysis - Glossary Entry - Genetics Home Reference
		Fragment of DNA of predictable size resulting from digestion (cutting) of a strand of DNA by a given restriction enzyme.
		Variation between individuals in DNA fragment sizes cut by specific restriction enzymes; polymorphic sequences that result in RFLPs are used as markers on both physical maps and genetic linkage maps.
		Variation in the length of a restriction fragment produced by a specific restriction enzyme acting on DNA from different individuals that usually results from a genetic mutation (as an insertion or deletion) and that may be used as a genetic marker -- called also RFLP.
	ghr.nlm.nih.gov /ghr/glossary/restrictionfragmentlengthpolymorphismanalysis (215 words)

	Untitled-3 (Site not responding. Last check: 2007-11-02)
		Restriction Enzymes (RE's) are exquisitely specific in their action -- they will only cut the nucleic acid if the exact sequence is recognized.
		This is the essence of RFLP analysis: my DNA will have accumulated different mutations like this than yours.
		Add to this the fact that at any given polymorphism site (a place where this type of difference occurs), one of your chromosomes may be different than the other, and you get a powerful tool not only in genetic counseling, but criminology as well.
	www.life.uiuc.edu /molbio/rflp/rflp.html (298 words)

	Energy Citations Database (ECD) - Energy and Energy-Related Bibliographic Citations
		Restriction fragment length polymorphism mapping of quantitative trait loci for malaria parasite susceptibility in the mosquito Aedes aegypti
		Susceptibility of the mosquito Aedes aegypti to the malarial parasite Plasmodium gallinaceum was investigated as a quantitative trait using restriction fragment length polymorphisms (RFLP).
		RFLP were tested for association with oocyst development on the mosquito midgut.
	www.osti.gov /energycitations/product.biblio.jsp?osti_id=91171 (296 words)

	Rapid identification of mycobacteria to species level by restriction fragment length polymorphism analysis of hsp65 ...
		Rapid identification of mycobacteria to species level by restriction fragment length polymorphism analysis of hsp65 gene.
		The sizes of the restriction fragments defined, are approximations, since they are determined by electrophoresis.
		Sequencing the PCR product, used in identification, for all the known species and production of a molecular weight marker that contain fragments the same size as all restriction fragments, may simplify this very practical method and help it to be established in routine clinical microbiology laboratories.
	www.aegis.com /aidsline/2000/jul/A0070888.html (496 words)

	Detection of the Finnish-Type Congenital Nephrotic Syndrome by Restriction Fragment Length Polymorphism and Dual-Color ...
		The prevalence of the polymorphism in the Finnish population was
		DNA sequence of the G3315A polymorphism region in exon 26.
		The sequence data of the antisense strand from an individual homozygous for the allele containing the polymorphism A3315 (A) and an individual homozygous for the WT allele (B) are shown.
	www.clinchem.org /cgi/content/full/46/6/811 (3585 words)

	Restriction Frament Length Polymorphism (Site not responding. Last check: 2007-11-02)
		Third, these fragments are separated in an agarose gel by a process called gel electrophoresis.
		From here, the fragments of DNA are transferred onto a nylon membrane and then are radioactively labeled with a probe in this single stranded state.
		By this time the probe has attached itself to complementary fragments, but the positions on the membrane are not visible to the human eye.
	www.louisville.edu /~jdgamb01/CapstoneRFLP.html (167 words)

	Restriction Fragment Length Polymorphism and Divergence in the Genomic Regions of High and Low Recombination in ... (Site not responding. Last check: 2007-11-02)
		Restriction Fragment Length Polymorphism and Divergence in the Genomic Regions of High and Low Recombination in Self-Fertilizing and Cross-Fertilizing Aegilops Species -- Dvorák et al.
		The length of each branch is proportionate to the number of character changes (above the branches) that have occurred since a preceding node.
		RFLP was investigated in 58 accessions of Ae.
	www.genetics.org /cgi/content/full/148/1/423 (5430 words)

	PCR-restriction fragment length polymorphism analysis of the ospC gene for detection of mixed culture and for ... (Site not responding. Last check: 2007-11-02)
		PCR-restriction fragment length polymorphism analysis of the ospC gene for detection of mixed culture and for epidemiological typing of Borrelia burgdorferi sensu stricto -- Masuzawa et al.
		PCR-restriction fragment length polymorphism analysis of the ospC gene for detection of mixed culture and for epidemiological typing of Borrelia burgdorferi sensu stricto
		Restriction fragment length polymorphism (RFLP) analysis of the outer
	cdli.asm.org /cgi/content/abstract/4/1/60 (455 words)

	IS1245 restriction fragment length polymorphism typing of Mycobacterium avium isolates: proposal for standardization.
		IS1245 restriction fragment length polymorphism typing of Mycobacterium avium isolates: proposal for standardization.
		Restriction fragment length polymorphism (RFLP) typing, using insertion sequence IS1245 as a probe, provides a powerful tool in the molecular epidemiology of M. avium-related infections and will facilitate well-founded studies into the sources of M. avium infections in animal and environmental reservoirs.
		The standardization of this technique allows computerization of IS1245 RFLP patterns for comparison on a local level and the establishment of M. avium DNA fingerprint databases for interlaboratory comparison.
	www.aegis.com /aidsline/1999/jan/A9910605.html (421 words)

	Restriction Fragment Length Polymorphism and Polymerase Chain Reaction-HLA DQa Analysis of ...
		Restriction Fragment Length Polymorphism and Polymerase Chain Reaction-HLA DQ a
		Hae III restricted samples were then resolved by analytical agarose gel electrophoresis, transferred to a membrane by Southern blotting and hybridized with a chemiluminescently-labelled (D2S44) probe.
		The DNA fragment banding patterns were indistinguishable from the DNA banding patterns of blood specimens collected from the same donor.
	www.astm.org /JOURNALS/FORENSIC/PAGES/1719.htm (463 words)

	PCR-Restriction Fragment Length Polymorphism Assay for Detection of gyrA Mutations Associated with Fluoroquinolone ... (Site not responding. Last check: 2007-11-02)
		PCR-Restriction Fragment Length Polymorphism Assay for Detection of gyrA Mutations Associated with Fluoroquinolone Resistance in Campylobacter coli -- Alonso et al.
		PCR-Restriction Fragment Length Polymorphism Assay for Detection of gyrA Mutations Associated with Fluoroquinolone Resistance in Campylobacter coli
		A fragment of the gyrA gene was sequenced from 34 isolates of
	aac.asm.org /cgi/content/full/48/12/4886 (1657 words)

	Misidentification of a genomovar of Burkholderia cepacia by recA restriction fragment length polymorphism -- Moore et ...
		Misidentification of a genomovar of Burkholderia cepacia by recA restriction fragment length polymorphism -- Moore et al.
		Figure 1 recA polymerase chain reaction restriction fragment length polymorphism patterns obtained with the query Burkholderia cepacia isolate (lane 1) isolated from an 8 year old girl with cystic fibrosis and B cepacia Genomovar IIIb reference strain (lane 2).
		Diverse restriction fragment length polymorphism patterns of the PCR-amplified 16S rRNA genes in Aeromonas veronii strains and possible misidentification of Aeromonas species.
	jcp.bmjjournals.com /cgi/content/full/55/4/309 (1708 words)

	PCR-restriction fragment length polymorphism analysis (PRA) of Mycobacterium leprae from human lepromas and from a ...
		PCR-restriction fragment length polymorphism analysis (PRA) of Mycobacterium leprae from human lepromas and from a natural case of an armadillo of Corrientes, Argentina
		A fragment of 439 bp was amplified with the primers Tb 11 and Tb 12 by the procedure described by Telenti, et al.
		The fragments obtained were separated by electro-- focusing on 3% agarose plus ethidium bromide and visualized under UV.
	www.findarticles.com /p/articles/mi_qa3754/is_200103/ai_n8937772 (648 words)

	Phylogenetic Analysis and PCR-Restriction Fragment Length Polymorphism Identification of Campylobacter Species Based on ... (Site not responding. Last check: 2007-11-02)
		RFLP analysis of the partial 517-bp groEL gene amplicon digested with AluI and ApoI.
		High-resolution genomic fingerprinting of Campylobacter jejuni and Campylobacter coli by analysis of amplified fragment length polymorphisms.
		Restriction fragment length polymorphism analysis shows that the hippuricase gene of Campylobacter jejuni is highly conserved.
	jcm.asm.org /cgi/content/full/42/12/5731 (3298 words)

	Restriction Fragment Length Polymorphisms (Site not responding. Last check: 2007-11-02)
		Because DNA is negatively charged, DNA fragments migrate to the positively charged end at a rate proportional to their relative size.
		For plant breeding, DNA can be isolated from experimental plants and their RFLP patterns determined with the DNA clones that are linked to important traits.
		Plants with the desirable RFLP pattern are identified and selected for further breeding, regardless of the environment in which the plants were grown.
	www.ag.uiuc.edu /~vista/html_pubs/irspsm91/fragment.html (411 words)

	Close correlation between restriction fragment length...
		Close correlation between restriction fragment length polymorphism of L-myc gene and susceptibility to gastric cancer
		METHODS: The distribution of L-myc polymorphism in 25 patients with gastric cancer was determined by polymerase chain reaction-based restriction fragment length polymorphism and compared with that of 83 healthy control subjects.
		RESULTS: We found a significant difference, both in the distribution of the LL, LS and SS genotypes and in the allelic frequencies, between the control group and the patient group; that is, the frequencies of L-myc alleles were, L and S, 0.52 and 0.48, 0.64 and 0.36, respectively.
	www.cancerprev.org /Journal/Issues/26/6/4760 (324 words)

	Restriction Fragment Length Polymorphism Analysis Reveals High Levels of Genetic Divergence Among the Light Organ ...
		Restriction Fragment Length Polymorphism Analysis Reveals High Levels of Genetic Divergence Among the Light Organ Symbionts of Flashlight Fish -- Wolfe and Haygood 181 (1): 135 -- The Biological Bulletin
		Restriction Fragment Length Polymorphism Analysis Reveals High Levels of Genetic Divergence Among the Light Organ Symbionts of Flashlight Fish
		Restriction fragment length polymorphisms within the lux and 16S ribosomal
	www.biolbull.org /cgi/content/abstract/181/1/135 (311 words)

	Rapid Molecular Diagnosis of Lactobacillus Bacteremia by Terminal Restriction Fragment Length Polymorphism Analysis of ...
		relative to the predicted fragments from the database.
		The single major 6-FAM (blue peaks) and HEX (green peaks) fragment of each digest are identified by their respective nucleotide size.
		Rapid identification of bacteria from positive blood cultures by terminal restriction fragment length polymorphism profile analysis of the 16S rRNA gene.
	www.clinmedres.org /cgi/content/full/2/1/37 (3222 words)

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