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Topic: Ribosomal RNA


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In the News (Thu 12 Nov 09)

  
  Non-coding RNA - Wikipedia, the free encyclopedia
Ribosomal RNA (rRNA) is the primary constituent of ribosomes.
Ribosomes are the protein-manufacturing organelles of cells and exist in the cytoplasm and attached to the membrane of the endoplasmic reticulum.
Efference RNA ( eRNA) is derived from intron sequences of genes or from non-coding DNA.
en.wikipedia.org /wiki/Non-coding_RNA   (1015 words)

  
 Nucleolin functions in the first step of ribosomal RNA processing
Ribosome biogenesis is a complex process which involves the transcription of a large rRNA precursor, its maturation and assembly with ribosomal proteins ( Hadjiolov, 1985 ; Eichler and Craig, 1994).
A radiolabeled RNA corresponding to nucleotides +541 to +1250 of the mouse rRNA (RNA
RNA was extracted from the second half of the processing reaction used in Figure 2A and analyzed on a denaturing gel ( Figure 2B).
www.nature.com /emboj/journal/v17/n5/full/7590862a.html   (8938 words)

  
 Mining Combinations of Structural Motif from SSU 16 S Ribosomal RNA Secondary Structures   (Site not responding. Last check: 2007-11-04)
Ribosomal RNA has been functionally implicated in virtually every aspect of protein synthesis and also provides the structural core for ribosomal assembly and is directly involved in the catalytic process.
The ribosomal RNA sequences were also widely used as a tool in molecular phylogenetic studies because of their ubiquity, size and low evolutionary rate [4].
For example, two imaginary RNA sequences, A and B, and drawings of corresponding secondary structures are shown in Figure 1 and the loop sequences separated by stems from 5' to 3' in the primary sequence.
www.bioinfo.de /isb/gcb01/poster/huang.html   (1328 words)

  
 Pfam 17.0 : Ribosomal_L11   (Site not responding. Last check: 2007-11-04)
Ribosomes are the particles that catalyze mRNA-directed protein synthesis in all organisms.
Many of ribosomal proteins, particularly those of the large subunit, are composed of a globular, surfaced-exposed domain with long finger-like projections that extend into the rRNA core to stabilize its structure.
Ribosomal protein L11 is one of the proteins from the large ribosomal subunit.
pfam.wustl.edu /cgi-bin/getdesc?name=Ribosomal_L11   (841 words)

  
 Re: What is Ribosomal RNA's purpose???
Ribosomes are the cytoplasmic particles which convert the base codes of all of the cell's mRNA's (the messages transcribed from genes) into protein sequences, to produce all of the proteins in the cell.
Each ribosome is composed of two parts: a smaller 40S (MW 1,400,000) subunit composed of the 18S rRNA species and 33 proteins; and a larger 60S (MW 2,800,000) subunit composed of three rRNA species - 28S, 5S, and 5.8S - and 49 proteins.
In fact, recent experiments have shown that there are several cases in which RNA molecules have catalytic activity and can act as enzymes.
www.madsci.org /posts/archives/dec96/847240135.Cb.r.html   (351 words)

  
 Processing of 20S pre-rRNA to 18S ribosomal RNA in yeast requires Rrp10p, an essential non-ribosomal cytoplasmic ...   (Site not responding. Last check: 2007-11-04)
Allmang,C., Mitchell,P., Petfalski,E. and Tollervey,D. (2000) Degradation of ribosomal RNA precursors by the exosome.
Carter,C.J. and Cannon,M. (1980) Maturation of ribosomal precursor RNA in Saccharomyces cerevisiae.
Finley,D., Bartel,B. and Varshavsky,A. (1989) The tails of ubiquitin precursors are ribosomal proteins whose fusion to ubiquitin facilitates ribosome biogenesis.
embojournal.npgjournals.com /cgi/content/full/20/15/4204   (7360 words)

  
 Specific Binding of Escherichia coli Ribosomal Protein S1 to boxA Transcriptional Antiterminator RNA -- Mogridge and ...
The indicated amount of S100 extract was incubated with radiolabeled boxA RNA (lanes 1 to 4) or reverse boxA RNA (lanes 5 to 8) and electrophoresed on a nondenaturing polyacrylamide gel.
Ribosomal RNA antitermination in vitro: requirement for Nus factors and one or more unidentified cellular components.
Torres, M., Condon, C., Balada, J.-M., Squires, C., Squires, C. Ribosomal protein S4 is a transcription factor with properties remarkably similar to NusA, a protein involved in both non-ribosomal and ribosomal RNA antitermination.
jb.asm.org /cgi/content/full/180/8/2248   (2596 words)

  
 Dbp6p Is an Essential Putative ATP-Dependent RNA Helicase Required for 60S-Ribosomal-Subunit Assembly in Saccharomyces ...
pre-mRNA splicing ( 48), ribosome biogenesis ( 67), and RNA
Total RNA was extracted, and 20,000 cpm was loaded and separated on a 1.2% agarose-formaldehyde gel, transferred to a nylon membrane, and visualized by fluorography.
Total RNA was extracted, and 30,000 cpm was loaded and separated on a 7% polyacrylamide-8 M urea gel, transferred to a nylon membrane, and visualized by fluorography.
mcb.asm.org /cgi/content/full/18/4/1855   (7810 words)

  
 Transcription
An enzyme, an RNA polymerase, binds to the complex of transcription factors.
In vitro, at least, when RNA polymerase is immobilized, it spins the DNA molecule around and around as it moves along the molecule.
RNA molecules that carry amino acids to the growing polypeptide.
users.rcn.com /jkimball.ma.ultranet/BiologyPages/T/Transcription.html   (1960 words)

  
 Dr Chromo's school: RNA structure
An RNA molecule is a linear polymer in which the monomers (nucleotides) are linked together by means of phosphodiester bridges, or bonds.
Although each RNA molecule has only a single polynucleotide chain, it is not a smooth linear structure.
ribosomal RNA or r RNA: constitutes 50% of a ribosome, which is a molecular assembly involved in protein synthesis.
www.rothamsted.bbsrc.ac.uk /notebook/courses/guide/rnast.htm   (696 words)

  
 Ribosomal Rna - Talk Medical
Ribosomal RNA: A molecular component of a ribosome, the cell's essential protein factory.
A tiny particulate structure located in the cytoplasm of the cell (outside the nucleus), the ribosome is composed of two subunits, one larger than the other.
Molecular maps of the ribosome have revealed startling details about its structure that boost support for an "RNA world" as the model for the origin of life on Earth.
www.talkmedical.com /medical-dictionary/12416/Ribosomal-Rna   (207 words)

  
 Pfam 17.0 : Ribosomal_S8   (Site not responding. Last check: 2007-11-04)
Ribosomal protein S8 is one of the proteins from the small ribosomal subunit.
In Escherichia coli, S8 is known to bind directly to 16S ribosomal RNA.
It belongs to a family of ribosomal proteins which, on the basis of sequence similarities MEDLINE:, groups eubacterial, algal and plant chloroplast, cyanelle, archaebacterial and Marchantia polymorpha mitochondrial S8; mammalian and plant S15A; and yeast S22 (S24) ribosomal proteins.
pfam.wustl.edu /cgi-bin/getdesc?name=Ribosomal_S8   (384 words)

  
 Rates of Ribosomal RNA Evolution Are Uniquely Accelerated in Eukaryotes
The results in this paper show that the molecular clock of ribosomal RNA from Eukaryotes is uniquely accelerated and highly variable while those of Archaea and Bacteria are not.
The sensor is at the level of DNA, not RNA, thus it is a mechanism that probably doesn't affect functional constraint of rRNA.
Morin L (2000) Long branch attraction effects and the status of “basal eukaryotes”: phylogeny and structural analysis of the ribosomal RNA gene cluster of the free-living diplomonad Trepomonas agilis.
www.dcn.davis.ca.us /vme/hgt/body.html   (3755 words)

  
 Ribosomal RNA Modifications   (Site not responding. Last check: 2007-11-04)
Posttranscriptional ribosomal RNA modification is common in all branches of the tree of life.
Base methylation is the best conserved in total number and position among all species, with bacteria containing slightly more than the 10 commonly found in eukaryotes (see Table 1.1).
Ribose methylation, always occurring at the 2' hydroxyl position on the sugar backbone (see Figure 1.2), is frequent in eukaryotes and very limited in bacteria (Table 1.1).
lowelab.ucsc.edu /~lowe/thesis/node15.html   (417 words)

  
 Conserved primer sequences for PCR amplification and sequencing from nuclear ribosomal RNA
The nuclear-encoded ribosomal RNA genes (rDNA) of fungi exist as a multiple-copy gene family comprised of highly similar DNA sequences (typically from 8-12 kb each) arranged in a head-to-toe manner.
Depending on the orientation and position of the 5S RNA gene, the PCR may be used to sequentially amplify either aportion of the intergenic spacer region (IGS) beyond the large subunit RNA coding region.
The small subunit ribosomal RNA gene sequences from the hypotrichous ciliates Oxytricha nova and Stylonychia pustula.
www.biology.duke.edu /fungi/mycolab/primers.htm   (1102 words)

  
 The Structure of the 16S Ribosomal RNA   (Site not responding. Last check: 2007-11-04)
PROTEAN is able to model the structure of a large ribonucleoprotein complex (the central domain of the 30S ribosomal subunit) from a combination of constraints derived from sequence analysis and experiments.
The 30S subunit consists of a single strand of RNA (the 16S rRNA, 1542 bases), and 21 proteins ranging in molecular weight from 9 kD to 61 kD.
The initial model of the central domain of the 16S ribosomal RNA structure is contained as a sample input to the display program, proteanD.
smi-web.stanford.edu /projects/helix/ribo.html   (666 words)

  
 IMB Jena Image Library: Index of RNA structures
If you are aware of RNA structures deposited at the PDB or NDB but not included here, let me know.
RNA / HNA hybrids (HNA - hexitol nucleic acids are oligonucleotides with a six-membered carbohydrate moiety)
All RNA containing entries are alse included in the compilations shown above.
www.imb-jena.de /IMAGE_RNA.html   (1074 words)

  
 [No title]   (Site not responding. Last check: 2007-11-04)
CCMP1838 small subunit ribosomal RNA gene, partial sequence; internal transcribed spacer 1, 5.8S ribosomal RNA gene, and internal transcribed spacer 2, complete sequence; and large subunit ribosomal RNA gene, partial sequence.
CCMP1835 small subunit ribosomal RNA gene, partial sequence; internal transcribed spacer 1, 5.8S ribosomal RNA gene, and internal transcribed spacer 2, complete sequence; and large subunit ribosomal RNA gene, partial sequence.
CCMP1828 small subunit ribosomal RNA gene, partial sequence; internal transcribed spacer 1, 5.8S ribosomal RNA gene, and internal transcribed spacer 2, complete sequence; and large subunit ribosomal RNA gene, partial sequence.
ccmp.bigelow.org /SD/ncbicites.txt   (13553 words)

  
 The European ribosomal RNA database
This database compiles all complete or nearly complete SSU (small subunit) and LSU (large subunit) ribosomal RNA sequences.
The European ribosomal RNA database was founded at the University of Antwerp (BELGIUM) in 1983.
As of this month the database will be maintained at the University of Ghent (also in BELGIUM).
www.psb.rug.ac.be /rRNA   (377 words)

  
 Rna, Ribosomal - Talk Medical
Both subunits were believed to contain both rRNA and protein but this is not the full story.
This suggests that the ribosome is actually a ribozyme, an RNA molecule that can catalyze its chemical reactions.
RNA's starring role in the ribosome may therefore support the idea that life on Earth began with RNA.
www.talkmedical.com /medical-dictionary/12468/Rna-Ribosomal   (207 words)

  
 16S and 23S Ribosomal RNA Mutation Database
Access to the expanded versions of the 16S and 23S Ribosomal RNA Mutation Databases has been improved to permit searches of the lists of alterations for all the data from (1) one specific organism, (2) one specific nucleotide position, (3) one specific phenotype, or (4) a particular author.
We plan to expand the database to include mutations in ribosomal proteins and ribosomal factors.
We are grateful to Janan Eppig, Carol Bult, and Debra Krupke from the Bioinformatics Group at the Jackson Laboratory for their expert advice on the use of appropriate database software.
www.oup.co.uk /nar/database/summary/229   (258 words)

  
 5S Ribosomal RNA Data Base   (Site not responding. Last check: 2007-11-04)
The crystal structure of Haloarcula marismortui large ribosomal subunit revealed deatails of three dimensional oganization of the 5S ribosomal RNA molecule.
3-dimensional structue of Haloarcula marismortui 5S ribosomal RNA in large ribosomal subunit The orientation of the molecule and colours of the domains correspond to the respective domains in the secondary structure diagram presented below.
Secondary structure of H.marismortui 5S rRNA observed in the crystal structure of large ribosomal subunit.
biobases.ibch.poznan.pl /Structure.html   (70 words)

  
 IMB Jena Biocomputing Group: The RNA World Website
Visualization of Viruses (DNA and RNA)- University of Wisconsin, Madison
Ribosomal Database Project II Ribosomal RNA Mutational Database
RNA secondary structure prediction server (Moscow State University)
www.imb-jena.de /RNA.html   (675 words)

  
 5S Ribosomal RNA Database
5S ribosomal RNA is an integral component of the large subunit of all cytoplasmic and most organellar ribosomes.
Its small size and association with ribosomal as well as non-ribosomal proteins made it an ideal model RNA molecule for studies of RNA structure and RNA-protein interactions.
5S Ribosomal RNA Database provides information on nucleotide sequences of 5S rRNAs and their genes.
www.oup.co.uk /nar/database/summary/230   (118 words)

  
 Vassar Ribosomal RNA Conference   (Site not responding. Last check: 2007-11-04)
In the spring of 1987, I sponsored a small conference on ribosomal RNA research.
Since then the conference has been an annual event.
However in the springs of 1991 and 1992 the conference sites were Brown University and Haverford College.
faculty.vassar.edu /jemiolo/ribosome.html   (57 words)

  
 Listing of RNABase Entries
Displaying 1-10 of 182 ribosomal RNA containing structures determined by all techniques
**Please note that while detection of RNA is done explicitly, assignment of a structure into a category (tetraloop, ribosomal RNA, etc.) is based only on the information provided by the authors.
Identification Of Known Protein And Rna Structures In A 5 A Map Of The Large Ribosomal Subunit From Haloarcula Marismortui
www.rnabase.org /listing?cat=rrna   (184 words)

  
 Ribosomal Database Project II - Release 9
The Ribosomal Database Project (RDP) provides ribosome related data and services to the scientific community, including online data analysis and aligned and annotated 16S rRNA sequences.
Release 9 of the RDP is designed to deliver up-to-date data to our users in an era of cheap sequencing.
PRIMROSE, a Program for Phylogenetic Probe Design, is Now Available [ more ]
rdp.cme.msu.edu   (312 words)

  
 problem5   (Site not responding. Last check: 2007-11-04)
Two sequences are the 16S ribosomal RNA gene of Mycobacterium.
gbM20940MSGTGDA M.bovis 16S ribosomal RNA and 23S ribosomal R...
Mycobacterium avium isolate M71 16S ribosomal RNA (rrs)
www.geocities.com /snongluk/E2.htm   (870 words)

  
 Land Plants Online - Ribosomal RNA Structures
Plant rRNA secondary structures are also available via the Ribosomal RNA Structural Database in Colorado maintained by R. Gutell.
The Ribosomal Database Project, formerly maintained at the University of Illinois at Urbana-Champaign, has now moved to Michigan State University.
A very complete list of algal and land plant nuclear 18S rDNA sequences can be obtained from Volker Huss:
www.science.siu.edu /landplants/rRNA/rRNA.html   (315 words)

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