| | BioMed Central | Full text | NO-independent regulatory site of direct sGC stimulators like YC-1 and BAY 41-2272 (Site not responding. Last check: 2007-10-12) |
 | | The enzyme is strongly activated by NO [7], by the new direct and NO-independent stimulator YC-1 [8-11], and to a lesser extend by CO Thus in several studies YC-1 was shown to be an antithrombotic agent by elevation of cGMP, VASP phosphorylation and inhibiting platelet aggregation [8,13,15-18]. |
 | | Interestingly, in addition to the direct activation of the purified sGC by YC-1, an overadditive effect was observed by the combinations of YC-1 and NO or CO It was shown that YC-1 is a heme-dependent but NO-independent stimulator of sGC [11,13]. |
 | | In forward to avoid an unproductive destruction of the PAL during the labeling of the enzyme [34], stimulation of sGC was not only tested under standardized conditions [11] but also verified under modified conditions in the absence of DTT. |
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