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Topic: Selective medium

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	Introduction to Cultivation of Bacteria
		The differential medium is one, which enables one to differentiate two types of organisms by their characteristic growth.
		Selective medium is one that contains ingredients that affronter advantage to particular organism over others by all growth of the particular organism and inhibiting the others.
		Enrichment medium is a liquid medina, which enema cues dm elm with of certain bacterial species, while show(ongoing the lag phase of tm~ ~gainers thus altering the rat two in favors of the required bacterial species.
	www.microbiologyguide.com /Cultivation_of_Bacteria.asp (465 words)

	Growth medium - Wikipedia, the free encyclopedia
		When agar, a substance which sets into a gel, is added to a liquid medium it can be poured into petri dishes where it will solidify, called agar plates, and provide a solid medium on which microbes may be cultured.
		Selective media are used to grow cells which possess a desired selectable trait.
		While selective media are used to allow the growth of only select microoganisms, differential media allow the growth of multiple types, but result in distinguishing characteristics (such as the production of a brightly coloured or phosphorescent dye).
	en.wikipedia.org /wiki/Microbiological_medium (603 words)

	Selective medium for Rhizoctonia (Site not responding. Last check: )
		A Semi-selective Medium to Isolate Rhizoctonia solani from Soil and Tissue
		A semi-selective medium was developed to isolate Rhizoctonia solani fron soil samples and infected tissue.
		This medium is effective to isolate and to clean colonies of most of the anastomosis groups of Rhizoctonia solani.
	www.ces.ncsu.edu /depts/pp/notes/Tobacco/tdin007/tb07Rizoc-AWA.html (239 words)

	BIOLOGICAL CHARACTERS
		Wilson and Blair’s brilliant-green bismuth sullphite agar (BBSA): The medium is highly selective for salmonellae, being inhibitory to coliforms, proteus and shigellae and is particularly useful in the isolation of S.typhi.
		Salmonella Shigella (SS) agar: SS agar is highly selective medium formulated to inhibit the growth of most coliform organisms and permit the growth of species of Salmonella and Shigella from environmental and clinical specimens.
		Various selective agents were screened for these capacities and a new medium was derived, named KIMAN (Whitley Impedance Broth basal medium supplemented with three selective components: potassium iodide, malachite green and novobiocin).
	www.geocities.com /avinash_abhyankar/biocharacters.htm (2405 words)

	M-Tech Diagnostics Ltd. Dehyrated and Liquid Media
		Medium for differential diagnosis and detection of E.coli, coliform bacteria and lactose-negative salmonelles and sgigelles in water, food and clinical samples.
		Medium for optimal detection of sublethaly damaged enteric bacteria, pseudomonas and staphylococci by preincubation followed by main incubation with selective media.
		Selective medium for yeasts and moulds in beverages and sugar.
	www.m-techdiagnostics.ltd.uk /media.shtml (939 words)

	Evaluation of a Selective Transport Medium for Gastric Biopsy Specimens To Be Cultured for Helicobacter pylori -- Siu ...
		Evaluation of a Selective Transport Medium for Gastric Biopsy Specimens To Be Cultured for Helicobacter pylori -- Siu et al.
		Evaluation of a Selective Transport Medium for Gastric Biopsy Specimens To Be Cultured for Helicobacter pylori
		In conclusion, the selective medium BHI-VAN is useful for prolonging the survival of H.
	jcm.asm.org /cgi/content/full/36/10/3048 (1681 words)

	Indicator medium - Wikipedia, the free encyclopedia
		Indicator medium (or indicator media) refers to the substance that microbiologists and geneticists use to grow bateria on that also contains some kind of material that acts as a signal to show the presence of chosen recombinant strains of bacteria.
		For example, in the presence of certain lac operon mutants, the chemical X-gal effects a change in the bacteria such that lac+ bacteria are blue and lac- bacteria are white.
		Other types of commonly used bacterial growth media include: minimal medium, complete medium, supplementary minimal medium, and selective medium.
	en.wikipedia.org /wiki/Indicator_medium (121 words)

	A New Selective Medium for Bifidobacterium spp. -- Nebra and Blanch 65 (11): 5173 -- Applied and Environmental ...
		medium (7) were not found on BFM agar (Table 4).
		Beerens, H. An elective and selective isolation medium for Bifidobacterium spp.
		Selective medium for isolation and enumeration of Bifidobacterium spp.
	aem.asm.org /cgi/content/full/65/11/5173 (2047 words)

	BBL Images
		Campy CSM (Charcoal-based Selective Medium) Agar is a medium for the primary isolation of Campylobacter spp.
		It is a selective, differential medium, inhibitory to gram-positive organisms.
		BBL Mycosel Agar is a selective medium for the isolation and cultivation of pahtogenic fungi, both yeasts and molds, from highly contaminated specimens.
	www.troybio.com /images/Product_Images_BBL/BBL.htm (6083 words)

	Patent 6,864,088
		After plating the mixture on a low-temperature selective medium by a low-temperature plating tool, the competent cells are cultured on the selective medium.
		C., the mixture is cultured on the selective medium to obtain competent cells.
		Finally, the mixture is cultured on the selective medium in an incubating unit to obtain transformed cells.
	www.pharmcast.com /Patents100/Yr2005/Mar2005/030805/6864088_Competent030805.htm (1943 words)

	Use of 3-aminotriazole to isolate oleate/acetate non-utilizing mutants of Aspergillus nidulans
		Oleate non-utilizing (olu) mutants of Aspergillus nidulans were isolated on selective medium containing the catalase inhibitor, 3-aminotriazole (3-AT); in an attempt to obtain strains that would enable analysis of peroxisome biogenesis and function in filamentous fungi.
		The selection procedure is based on the lethality of H2O2 formed during peroxisomal -oxidation of fatty acids in the presence of the catalase inhibitor 3-aminotriazole (3-AT).
		The selection medium should contain: at least, two different carbon sources: a fatty acid, to induce peroxisomal fatty acid oxidation, and a second carbon source (other than a fatty acid) to allow growth of mutants disturbed in peroxisomal fatty acid oxidation.
	www.fgsc.net /fgn43/delucas.html (1020 words)

	Patent 6,969,593
		The selectivity is determined by the selective character of the antibody in recognising the target cell compared to the cross reactivity of this antibody towards other non-target cells.
		This rich medium is additionally supplemented with a specific A-B complex composed of a specific antibody or antibody derivative A and a well-chosen biotic agent B (step d in a preferred embodiment of the present invention).
		The present invention also describes a selection medium for cells comprising a conjugate A-B, a corresponding anti-bios agent able inhibit the growth of the mixture of cells and additives stimulating the growth of the target cells.
	www.pharmcast.com /Patents100/Yr2005/Nov2005/112905/6969593_TargetCell112905.htm (5025 words)

	Whatman - Leadership in separations technology for the life sciences
		The pad is a stable carrier which does not alter the nutrient medium or influence the growth of the microorganisms in any way.
		Selective medium for the detection of fecal streptococci in water, food, and other test materials.
		Growth or positive reaction to selective medium should be regarded with suspicion.
	www.whatman.com /products?pageID=7.61.409.298 (551 words)

	BD - Diagnostic Systems: BCYE Selective Agar with PAC
		BCYE Selective Agar w/ CCVC is a highly selective medium consisting of BCYE Agar supplement with cephalothin, colistin, vancomycin and cycloheximide.
		pneumophila by using the selective medium in conjunction with an acid wash treatment to reduce the contaminating microbial flora present in environmental water samples.
		BCYE Selective Agar with PAV is similar to the Edelstein formula, above, except that the concentration of polymixin B is reduced by half, and vancomycin is substituted for cefamandole.
	www.bd.com /ds/productCenter/297879.asp (957 words)

	Microbiology Primer: Agar types
		This medium is blood agar which has been enriched by lysing the red blood cells and making their contents available to organisms growing on the medium.
		This is a selective and differential medium for the cultivation of non-fastidious gram negative bacilli.
		This medium is made selective for fungi by the addition of antibacterial agents.
	www.aecom.yu.edu /id/micro/agar.htm (307 words)

	Microbes Forum > Differential vs selective media (Site not responding. Last check: )
		For example, Mannitol Salt Agar (MSA) is a medium that "selects" for the growth of halotolerant bacteria.
		A differential medium is one that distinguishes between defining characteristics of organisms.
		Mannitol Salt Agar is a selective AND differential medium.
	www.microbes.info /forums/lofiversion/index.php/t188.html (318 words)

	European Society of Clinical Microbiology and Infectious Diseases
		For Lac 8 selective media (LAMVAB, Sorbitol agar, 2 Rogosa variants and 4 MRS variants) were studied with 24 Lac strains and 14 faecal (non-Lac) strains.
		The MRS medium, most widely used for enumeration of Lac from faecal samples, is not selective (specificity = 0%) but sustains the growth of all Lac strains tested (sensitivity = 100%).
		MTPY is the optimal medium for Bif and BBE is the medium of choice for selective enumeration of Bac.
	www.blackwellpublishing.com /eccmid15/abstract.asp?id=36566 (377 words)

	Culturing Microbes
		Selective medium is designed to suppress the growth of some microorganisms while allowing the growth of others (i.e., they select for certain microbes).
		Colony size is dependent not just on the type of organism but also on the growth medium and the number of colonies present on a plate (that is, colonies tend to be smaller when greater than ascertain amount are present) and on culture medium characteristics.
		A __________ medium is one that aids the growth of obligate anaerobes.
	www.mansfield.ohio-state.edu /~sabedon/biol4035.htm (1764 words)

	Solar Biologicals Urine Dipslide Information Document
		Use of different selective growth media permits simultaneous exposure of a urine specimen to a medium selective for gram-positive species on one side, for example, and to a medium selective for gram-negative species on the other.
		This highly selective medium is suitable for the detection, identification and enumeration of coliform organisms.
		EMB agar is a versatile medium that is recommended for the detection, identification and enumeration of members of the coliform group.
	solarbiologicals.com /clinical_dipslides.htm (950 words)

	Nutrition and Cultivation of Bacteria - Page 2
		The particular selective agents chosen for this medium – bile salts and crystal violet – inhibit gram-positive bacteria, allowing the near-exclusive growth of gram-negative bacteria.
		This medium is used for the enrichment of the purple non-sulfur photosynthetic bacteria; most other organisms tend not to metabolize succinate under the anaerobic conditions utilized.
		Cystine and thioglycollate are reducing agents often used for the cultivation of anaerobes and are found in the Thioglycollate Medium utilized to determine oxygen relationships.
	www.jlindquist.net /generalmicro/102bactnut2.html (1531 words)

	semi-selective medium
		This medium is a modification of the one reported by Steadman et al in 1995.
		Antibiotics and fungicides were added to the medium after sterilization (121 C at 15 psi for 30 minutes).
		To trap ascospores, petri dishes containing the blue medium should be left open for one or two hours, between 9 to 11 a.m.
	www.ces.ncsu.edu /depts/pp/notes/Tobacco/tdin006/Sclerotiniaselectivebluemedium.html (353 words)

	BSCI424 Laboratory Media
		Bile Esculin Agar (BEA): selective medium for the detection of fecal streptococci (group D) and enterococci; tests ability of the organism to hydrolyze esculin to esculetin.
		For clinical gonococcal specimens, isolates are subcultured from the selective primary medium to a noninhibitory enrichment medium, e.g., chocolate agar supplemented with 1% IsoVitaleX to obtain a pure culture of the isolate.
		Lysine Decarboxylase Medium: a yellow color indicates a low pH and that the test is negative (failure to produce an amine by decarboxylation of lysine).
	www.life.umd.edu /classroom/bsci424/LabMaterialsMethods/BSCI424Media.htm (1564 words)

	Improved, Low-Cost Selective Culture Medium for Actinobacillus actinomycetemcomitans -- Alsina et al. 39 (2): 509 -- ...
		Development of a selective medium for the specific recovery of a given pathogen is primarily based on the selection of an
		A selective medium for Actinobacillus actinomycetemcomitans and the incidence of the organism in juvenile periodontitis.
		Selective medium for the isolation of Haemophilus aphrofilus from the human periodontium and other oral sites and the low proportion of the organism in the oral flora.
	jcm.asm.org /cgi/pmidlookup?view=full&pmid=11158098 (2735 words)

	Identification of Essential Genes in the Human Fungal Pathogen Aspergillus fumigatus by Transposon Mutagenesis -- Firon ...
		During haploidization on a benomyl containing medium, random loss of chromosomes gives rise to two subpopulations of colored haploid conidia (w1 or r7): one bearing the transposon-inactivated allele (population A) and one bearing the wild-type allele (population B).
		Positive selection of transposition events is obtained by selection of nitrate-utilizing revertants, which appear as a result of the excision of imp::pyr and the restoration of a functional niaD promoter.
		Selection of imp::pyr reintegration events is ensured by the presence of pyrG in the transposable element when transposition events are induced in an A.
	ec.asm.org /cgi/content/full/2/2/247 (4711 words)

	Untitled (Site not responding. Last check: )
		A selective medium for the isolation of pathogenic staphylococci
		For the selective isolation of Salmonella with the exclusion of S.typhi and S.paratyphi
		A selective enrichment broth for the isolation of Salmonella spp.
	www.cosmosbiomedical.com /bacti/culturemedia/culturemedia2.shtml (806 words)

	3954enterics.html
		This is an effective medium for the rapid differentiation between lactose-fermenting and nonlactose-fermenting organisms in fecal specimens.
		-a medium relatively selective for Enterobacteriaceae and differential for lactose + and lactose - organisms.
		Colonies of lactose fermenters are distinguished by red colonies surrounded by a zone of precipitated bile acids; lactose non fermenters are “colorless.” A moderate inoculum is used.
	www.tarleton.edu /~jkonvicka/3954enterics.html (812 words)

	Mitotic instability in benomyl-resistant transformants of a fluffy strain of Neurospora crassa
		Homokaryotic derivatives from the presumably heterokaryotic primary transformants were obtained by isolating colonies grown from microconidia inoculated on selective (0.25 ug/ml benomyl) Vogel's medium N supplemented with 2% sorbose, 0.05% fructose and 0.05% glucose.
		The proportion of transformed nuclei and their stability in benomyl-resistant transformants were determined by genetic analysis of microconidia produced on selective (0.5 ug/ml benomyl) or non-selective acetate medium, assuming that the microconidia contain a random sample of the nuclei present in the mycelium.
		To investigate at the molecular level the effects of the suppression of selective pressure on the fate of the transforming DNA, 3 transformants (1f, 9 and 3i) were grown in Vogel's medium N plus (0.25 ug/ml) or minus benomyl.
	www.fgsc.net /fgn39/rossier.html (1155 words)

	Mycobiotic Agar (Site not responding. Last check: )
		This medium is recommended for the isolation of pathogenic fungi from clinical specimens which may be contaminated with bacteria and saprophytic fungi.
		It is a selective medium that is nearly neutral in reaction and contains the antibiotics cycloheximide and chloramphenicol.
		For isolation of fungi from potentially contaminated specimens, a nonselective medium should be inoculated along with the selective medium.
	dentistry.ouhsc.edu /intranet-Web/Courses/DMI_8351/MYCO.html (275 words)

	Acid Detection Test- Gonorrhea - STD information from CDC
		CTA media, contained in screw-capped tubes, are inoculated with heavy suspensions of a 18 h-24 h pure subculture from chocolate, or equivalent, medium.
		Clinical isolates may be subcultured from selective medium or purified subcultures.
		Negative reaction is recorded when the color in the test medium is the same, or a darker red, than the color in the control medium.
	www.cdc.gov /std/Gonorrhea/lab/tests/acid.htm (1951 words)

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