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Topic: Sequencing

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In the News (Mon 18 Dec 17)

  DNA Sequencing | Nucleics
In addition to being able to detect sequencing problems independent of the base caller used, the QualTrace™ analysis software also offers DNA sequencing core facilities a reproducible and cost efficient means of providing independent sequencing troubleshooting advice to their customers as to the cause of any sequencing problems.
The CounterTrace sequencing kit is compatible with both the ABI 3700 and 3730 DNA sequencers and has been designed to be used with the phred base caller.
The ASIN genome sequencing technology was sold to Takara Biomedicals, a division of Takara Shuzo (Japan) in February 2000.
www.nucleics.com /DNA_sequencing_tools/DNA_sequencing_tools.html   (1667 words)

 Davis Sequencing, an Automated DNA Sequencing Facility: Home Page
Davis Sequencing provides high-throughput automated DNA sequencing services to university, commercial and government customers worldwide.
Our goal is to provide a high quality DNA sequencing service at a low price with a fast turn-around time.
Davis Sequencing will be closed on Labor Day on Monday, September 3rd.
www.davissequencing.com   (77 words)

  Genome Sequencing
DNA sequencing, the process of determining the exact order of the 3 billion chemical building blocks (called bases and abbreviated A, T, C, and G) that make up the DNA of the 24 different human chromosomes, was the greatest technical challenge in the Human Genome Project.
Automated sequencers analyze the resulting electropherograms, and the output is a four-color chromatogram showing peaks that represent each of the four DNA bases.
This repeated sequencing is known as genome "depth of coverage." Draft sequence data are mostly in the form of 10,000 basepair-sized fragments whose approximate chromosomal locations are known.
www.ornl.gov /sci/techresources/Human_Genome/faq/seqfacts.shtml   (1853 words)

  Sequencing - Wikipedia, the free encyclopedia
In genetics and biochemistry, sequencing means to determine the primary structure (or primary sequence) of an unbranched biopolymer.
Sequencing results in a symbolic linear depiction known as a sequence which succinctly summarizes much of the atomic-level structure of the sequenced molecule.
In chain terminator sequencing (Sanger sequencing), extension is initiated at a specific site on the template DNA by using a short oligonucleotide 'primer' complementary to the template at that region.
en.wikipedia.org /wiki/Sequencing   (832 words)

 sequencing - Hutchinson encyclopedia article about sequencing
Techniques for sequencing amino acids in proteins were established in the 1950s, insulin being the first for which the sequence was completed.
Scientists are now able to routinely determine the genome of organisms as small as viruses and the more complex systems of plants and mammals.
In July 2001, the Global Musa Genomics Consortium, which is made up of publicly funded institutes, announced that the banana was to become the first fruit to have its genome sequenced.
encyclopedia.farlex.com /sequencing   (191 words)

 DNA sequencing
DNA sequencing is the process of determining the exact order of the bases A, T, C and G in a piece of DNA.
Sequencing is achieved by including in each reaction a nucleotide analogue that cannot be extended and thus acts as a chain terminator.
Lower-fidelity single-pass sequencing is useful for the rapid accumulation of sequence data at the expense of some accuracy.
www.wellcome.ac.uk /en/genome/technologies/hg17b003.html   (607 words)

 Sequencing   (Site not responding. Last check: )
These standards consider quality aspects of the whole process of sequencing and make the assumption that the analytical process takes place in an appropriate, accredited laboratory setting where routine aspects of good laboratory practice such as sample tracking and record keeping are in place.
Sequencing template may not be routinely checked prior to sequencing unless difficulties arise during the sequencing process.
With single lane sequencing using four colour dyes, the data are processed by proprietary colour separation techniques to generate traces corresponding to an autoradiograph.
www.cmgs.org /BPG/Guidelines/2002/sequence.htm   (2027 words)

 HGSC Sequencing Projects
The HGSC is sequencing the genome of the tammar wallaby (Macropus eugenii) as part of the Tammar Wallaby Genome Sequencing Consortium, a collaboration between the HGSC and the Australian Genome Research Facility Ltd. (AGRF).
The HGSC is sequencing the genome of the white-tufted-ear Marmoset (Callithrix jacchus).
The HGSC is sequencing the pea aphid Acyrthosiphon pisum.
www.hgsc.bcm.tmc.edu /projects   (1162 words)

 Music sequencer - Wikipedia, the free encyclopedia
Many sequencers have features for limited music notation, or are able to show music in a piano roll notation.
Though the term 'sequencer' is today used primarily for software, some hardware synthesizers and almost all music workstations include a built-in MIDI sequencer.
Music can be sequenced in a sense using trackers such as ModPlug Tracker, and a drum machine can be viewed as a specialized music sequencer.
en.wikipedia.org /wiki/Music_sequencer   (366 words)

 Sequencing Info - Encyclopedia WikiWhat.com   (Site not responding. Last check: )
In genetics, sequencing means to determine the nucleotides of a DNA strand.
In biochemistry, sequencing means more generally the determination of the primary sequence of any linear heterobiopolymer.
This includes nucleotide sequencing (for DNA or RNA) as well as protein sequencing (using Edman degradation, mass spectrometry, protease digests).
www.wikiwhat.com /encyclopedia/s/se/sequencing.html   (181 words)

 [No title]
Each base-specific sequencing reaction terminated with the short termination mix is loaded using a mouth pipette onto a 0.15 mm X 50 cm X 20 cm, denaturing 5% polyacrylamide gel and electrophoresed for 2.25 hours at 22 mA.
One of the major problems in DNA cycle sequencing is that when fluorescent primers (1) are used the reaction conditions are such that the nested fragment set distribution is highly dependent upon the template concentration in the reaction mix.
Sequencing with these primers results in a long poly(T) ladder followed by a sequence which is difficult to read.
www.genome.ou.edu /protocol_book/protocol_partIV.html   (5333 words)

Despite modern technology, the principles remain unchanged: sequencing records a musician’s performance (the notes and their timing), not the actual sound of the music.
The earliest sequencing device used in electronic music was the step sequencer, as used in conjunction with a voltage controlled synthesiser.
Sequencing software allowed the musician to record a real-time keyboard performance as a sequence in the computer.
myweb.tiscali.co.uk /whitefiles/b2_h/4_radiophonic_workshop/rws/pgs/rx03.htm   (1172 words)

 Biopharmaceutical Sequencing glossary
Sequencing was limited to only 200- 300 bases of data from a single sample analysis and many reiterative steps were required to acquire just a few thousand bases of DNA sequence data.
Sequencing of biomolecules began with the insulin B-chain - a thirty residue peptide - which Saenger and Tuppy deduced through a combination of limited proteolysis and chemical analysis in 1951.
Sequencing method which involves randomly sequencing tiny cloned pieces of the genome, with no foreknowledge of where on a chromosome the piece originally came from.
www.genomicglossaries.com /content/sequencing_gloss.asp   (6321 words)

 Protein Sequencing
Two instruments are currently used in the facility: the 477A protein/peptide sequencing system with an in line PTH-analyzer from Applied Biosystems and a Porton Instrument with an in line PTH analyzer from Beckman Coulter.
Protein/peptide sequencing is performed by the Edman chemistry and involves the removal of one amino acid each cycle from the N-terminus of the peptide.
Non-amine buffers are tolerated by the sequencer in the 1-5 mM range; amine buffers such as Tris are not compatible with th Edman reactions.
www.biochem.mcw.edu /protein_facility/protein_sequencing.html   (279 words)

 Protein Sequencing   (Site not responding. Last check: )
The automated sequencer in the Biopolymers Laboratory is capable of obtaining approximately 15 residues of sequence from as little as 3 pmoles of sample.
Sequencing chemistry begins with immobilization of protein onto a glass fiber disc followed by coupling phenylisothiocyanate (PITC) to the N-terminal amine group of the polypeptide chain to form a phenylthiocarbamyl (PTC) protein.
If the amount of protein to be sequenced is limited and you are concerned about sample loss during reduction and alkylation, you can sequence non-derivatized in the hope that there are no cysteine residues in the sequence since cysteine is a relatively rare amino acid.
web.mit.edu /biopolymers/www/protein_sequencing.html   (8226 words)

 DNA Sequencing: Human Genome Research in Progress
One of the primary research areas was DNA sequencing.
Sequencing, Sequencing Resources, and Sequencing Technologies abstracts from 2002 U.S. DOE Human Genome Program Contractor-Grantee Workshop IX Sequencing and
Researchers use these markers as "sequence tag connectors" (STCs) to identify the specific clones needing to be sequenced to extend sequenced regions further along the chromosomes and for other uses in large-scale sequencing efforts.
www.ornl.gov /hgmis/research/sequencing.html   (667 words)

 Teachers: Content Literacy
Of all the text structures, sequencing is the easiest for students to understand.
Students see many examples of sequencing in their lives and have read many stories in which the order of the story makes a difference.
This lesson plan provides a sequencing activity in which the students narrow down the story's key events and then create a sequencing quiz to go with the story.
www.literacymatters.org /content/text/sequence.htm   (435 words)

 NHGRI Expands Effort to Revolutionize Sequencing Technologies, August 8, 2005 Press Release - National Institutes of ...
NHGRI’s near-term goal is to lower the cost of sequencing a mammalian-sized genome to $100,000, which would enable researchers to sequence the genomes of hundreds or even thousands of people as part of studies to identify genes that contribute to common, complex diseases.
NHGRI’s “Revolutionary Genome Sequencing Technologies” grants have as their goal the development of breakthrough technologies that will enable a human-sized genome to be sequenced for $1,000 or less.
This group is developing a sequencing system in which polymerase (an enzyme used to synthesize DNA molecules) and nucleotides act together as direct molecular sensors of DNA base identity.
www.nih.gov /news/pr/aug2005/nhgri-08.htm   (1210 words)

 DNA Sequencing
The primary objective of the DNA sequencing shared resource is to expedite the research efforts of both basic science and clinical investigators at McGill University, to provide accurate, low cost DNA sequencing and to save time in the production of data by replacing older, inefficient and costly manual methods with state-of-the art, automated procedures.
The DNA sequencing shared resource is intended to lower its service charges to provide a cost-effective centralized laboratory and reduce the need for individual laboratories to purchase expensive sequencing equipment, thus reducing radioactive waste.
Primer walk sequencing is the method for sequencing DNA fragments between 1.0 and 15.0 kb.
www.mcgill.ca /sheldon/dna_seq   (2368 words)

 University of Washington Genome Center
This site describes our current and past projects that include whole genome sequencing of microbes, targeted sequencing and resequencing of humans and primates genomes, studies of genetic variation among strains of microbes, in humans and among many different species, and studies of how and where viral intergration sites occur in cells used in gene therapy.
As a member of the I nternational Human Genome Sequencing Consortium, we helped sequenced the human genome and are still involved with correcting and refining the reference sequence by closing gaps in the draft sequence.
For example, we have sequenced and analyzed the haplotypes of the highly variable HLA Class II Region (for 22 human and primates) and are sequencing and analyzing 30 human haplotypes in a 420kb region around the Cystic Fibrosis (CFTR) gene.
www.genome.washington.edu /UWGC   (534 words)

 JST Humen Genome Sequencing Home Page
Sequencing data carried out by this projects is available here.
JST Sequencing Project is in progress on the following 7 chromosomes and sequencing is conducted by four sequencing teams contracted with JST.
Maps and sequencing data are shown via either selection of a chromosome name or a cytogenetic region through the table.
www-alis.tokyo.jst.go.jp /HGS/top.pl   (299 words)

 Automated DNA Sequencing   (Site not responding. Last check: )
Automated DNA sequencing is performed by a Perkin-Elmer (Applied Biosystems) Model 373 XL DNA sequencing system.
Sequencing reactions prepared by thermocycle sequencing are resolved and read in a single lane, rather than four separate lanes, enabling a much higher throughput of DNA sequence data.
These enhancements increase the well-to-read distance (the distance a DNA fragment migrates through the separating gel before laser excitation and detection) from 24 cm to 34 or 48 cm allowing about 100 or 200 extra bases to be read, respectively, at no extra expense to our customers.
opbs.okstate.edu /CORE/AutoDNASeq.html   (337 words)

 biology - Sequencing
In genetics terminology, sequencing is determining the nucleotides of a DNA or RNA strand.
Currently, all sequencing is performed using the chain termination method, created by Frederick Sanger.
Two of the mainstream chain termination strategies are chromosome walking and shotgun sequencing.
www.biologydaily.com /biology/Sequencing   (231 words)

 Sequencing   (Site not responding. Last check: )
We recognize the needs of the growing number of mothers who move in and out of paid employment and/or opt for a variety of flexible work arrangements in order to successfully balance their work and family responsibilities.
This fluid work pattern, which occurs over a number of years and at various stages of motherhood, is known as “sequencing”.
Regardless of the many individual ways to combine paid employment and motherhood, sequencing women are united by common experiences – which include, either by choice or necessity, adapting their work arrangements to better accommodate the responsibilities of family care.
www.mothersandmore.org /Sequencing/sequencing.htm   (254 words)

I believe that sequencing is also easier with three kinds of "knowing" firmly in place: know yourself, know your partner, and know your children.
Finally, if possible, consider another sequencing technique often associated with weaning: "don't offer, don't refuse." This frees you to stay open to possibilities that may present themselves without pressure to pursue paid employment.
Remember that few, if any, employment decisions are irreversible and that the road to successful sequencing is likely to be lined with detours, unexpected curves, and even an occasional dead end.
www.lalecheleague.org /NB/NBJulAug97p100.work.html   (3674 words)

 Automated DNA Sequencing
Automated DNA sequencing utilizes fluorescent tracers instead of radioisotopes to label the DNA, thereby eliminating or significantly reducing the use of radioactive materials in some research laboratories.
DNA sequencing is achieved by utilizing labeled nucleotides for incorporation into a copy of a piece of DNA.
It is important to note that factors that tend to increase the costs of manual DNA sequencing (e.g., the long operation time; associated tasks and personal protection required for working with radioactive material) are not included in the costs of automated DNA sequencing.
www.p2000.umich.edu /chemical_waste/cw12.htm   (1025 words)

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