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Topic: Serial clones


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In the News (Mon 8 Sep 08)

  
  Dolly the sheep - Wikipedia, the free encyclopedia
She was cloned at the Roslin Institute in Scotland and lived there until her death at the age of six.
The arthritis further fueled worry among some that this form of cloning may not be appropriate for mammals, and there is now a consensus both within and outside the scientific community that at this point the risk of unforeseen effects of cloning on the clone makes experiments in human reproductive cloning premature and unethical.
Cloning has already become an option for preserving animals that may become extinct and bringing back to life replicas of pets, such as cats and dogs.
www.wikipedia.org /wiki/Dolly_the_sheep   (832 words)

  
 Encyclopedia: Serial clones
A Serial Clone is defined as a clone of a clone.
However, unlike a xerox copy these clones are as good as the original (barring any genetic mutations).
Serial Clones are referred to by generation with 1st generation denoting the 1st clone, on to Nth generations.
www.nationmaster.com /encyclopedia/Serial-clones   (112 words)

  
 Serial bull cloning by somatic cell nuclear transfer - Nature Biotechnology
Serial bull cloning, in addition to its potential practical import, would provide an excellent model to study the effects of progressively accumulated somatic mutations on development, health and reproductive performance.
The birth weights of both G2 clones (44 and 42 kg), as well as their donor, G1-C, were more than 40% greater than the breed average for male calves (30 kg) and that of the original donor, G0 (30 kg).
Because serial somatic cell cloning has not been reported in cattle and telomere lengths in serial bovine clones are not known, we compared the mean telomere lengths in fibroblasts and blood leukocytes from all G1 and G2 clones by terminal restriction fragment assays (TRF, Fig.
www.nature.com /cgi-taf/DynaPage.taf?file=/nbt/journal/v22/n6/full/nbt975.html   (1477 words)

  
 ipedia.com: Dolly the sheep Article   (Site not responding. Last check: 2007-10-19)
The particular technique that was made famous by her birth, is somatic cell nuclear transfer, in which the nucleus from one of the donor's non-reproductive cells, is placed into a de-nucleated embryonic cell (which is then coaxed into developing into a fetus).
When Dolly was cloned in 1996 from a cell taken from a 6 year old ewe, she became the centre of a controversy that still continues today.
It is arguable that Dolly is perhaps the perfect example of scientists focusing in on highly specialised areas, to such an extent that they are no longer able to see the wider picture, and for that matter, the implications of their endeavours.
www.ipedia.com /dolly_the_sheep.html   (922 words)

  
 Serial Transplantation of Embryonic Nuclei.
One member of such a clone may be sacrificed at the blastula stage to provide nuclei for transfer to a new group of enucleated eggs, giving in effect a second blastula generation of the clone.
In some clones all embryos were arrested at the gastrula stage, in others they displayed a fairly uniform set of deficiencies in post-gastrula development, and in a few clones almost all embryos developed normally throughout.
Furthermore, within any given clone the development in the second and later generations were generally of the same type as that observed in the first generation.
www.euchromatin.org /King01.htm   (506 words)

  
 Wired News: Bullish Hopes for Serial Cloning   (Site not responding. Last check: 2007-10-19)
Researchers created six clones of the bull in 2000, and now they have also cloned one of those six in the first successful "serial cloning" experiment with a large mammal.
By the time they cloned him, he was 17 years old (the lifespan for bulls is typically around 20 years), so he was approaching the end of his tenure as a master breeder.
While the cell lines used to create the first-generation clones were old because they were taken from an old bull, cloning the first-generation clones gave the researchers cells that were genetically identical, but young.
www.wired.com /news/print/0,1294,63545,00.html   (954 words)

  
 RHIZOPON - Effect of age on maturation of loblolly pine clones maintained by hedging and serial propagation   (Site not responding. Last check: 2007-10-19)
The primary needle length changed with clone age in a pattern that was quite similar to the rooting results.
After two growing seasons, clone age significantly affected tree height, but only the rooted cuttings from clones five years of age or older were significantly shorter than seedlings.
Because the rooted cuttings from older clones were shorter than those from younger clones and seedlings at the time of planting and after the first growing season, we calculated second-year height growth increm ent.
www.rhizopon.nl /rootformation2004/edit.Effectofageonmaturationofloblollypineclonesmaintainedbyhedgingandserialpropagation.shtml   (423 words)

  
 Extensive in vivo self-renewal, long-term reconstitution capacity, and hematopoietic multipotency of Pax5-deficient ...
serial transplantations, with in vitro recloning of the bone marrow-homed
pre-B I cell clones is shown in Figure 1 with data obtained by cell counting in vitro.
Serial depletion and regeneration of the murine hematopoietic system: implications for hematopoietic organization and the study of cellular aging.
www.bloodjournal.org /cgi/content/full/99/8/2760   (4325 words)

  
 Standards and specs: Naturally occurring standards   (Site not responding. Last check: 2007-10-19)
For instance, the clones of the original IBM® PC have left us with machines with 3Ghz processors and 4GB of RAM which still distinguish between the 640K of "base" memory and the almost-4GB of "extended" memory.
The biggest danger of clone standards is probably the comparative lack of documentation, especially in boundary conditions.
The Yost Serial Device Wiring Standard is a pretty good de facto standard that solves four of six RS-232 hassles (cable connectors are all male, are all RJ-45, there is no distinction between DTE and DCE plugs/sockets, cables can be mass-terminated with a crimping tool).
www-128.ibm.com /developerworks/power/library/pa-spec6.html   (4294 words)

  
 NYPOST.COM Movie Reviews: FORCEFUL RETURN OF GEORGE LUCAS' SERIAL THRILLER By LOU LUMENICK   (Site not responding. Last check: 2007-10-19)
But on a purely visceral level, "Clones" is a delightfully rousing, eye-popping, crowd-pleasing homage to Saturday-morning serials of the '30s and '40s.
Fans of the series will gladly endure their somewhat excruciating "Titanic"-style love scenes - with lovely Lake Como, Italy, standing in for Naboo - as a small price to pay for seeing key events leading up to the future advent of Padme and Anakin's twin children, Luke Skywalker and Princess Leia.
Obi-Wan is dispatched to a distant water world where he discovers an army of clones has been assembled, supposedly com-missioned by the Republic to help battle the separatists.
www.nypost.com /movies/051202.htm   (687 words)

  
 Juvenility and serial vegetative propagation of Norway spruce clones (Picea abies Karst.)
Serial propagation has been in use at the Lower Saxony Forest Research Institute since 1968 to arrest maturation in Norway spruce clones.
By 1980 cuttings were established in the nursery that had been serially propagated from one to five cycles.
Serial propagation appears to have at least considerably slowed, and perhaps arrested, maturation processes in Norway spruce.
www.srs.fs.usda.gov /pubs/viewpub.jsp?index=4954   (269 words)

  
 Clonal dynamics of progressive neoplastic transformation -- Chow and Rubin 96 (12): 6976 -- Proceedings of the National ...
Selected assays from three of the clones are shown here to illustrate the diverse clone-specific dynamics of transformation during the low-density passages.
At the same time, the growth rate of the standard passage cells of each clone was determined, and the ratio of growth rate (PD/D) of the postconfluent passage to that of the standard passage of the clone at low density is shown.
the 1° assay of clone 1A in Fig.
www.pnas.org /cgi/content/full/96/12/6976   (4407 words)

  
 The Commodore serial cable   (Site not responding. Last check: 2007-10-19)
It's compatible with all Commodore machines and all Commodore drives and clones that have the usual serial bus port.
Connect the two serial plugs into the serial ports on your Commodore drive and your Commodore machine.
Put your equipment far away from monitors, TV sets and other devices with strong emission, otherwise cables may pick up interference and you may experience data loss or corruption.
sta.c64.org /cbmser.html   (270 words)

  
 Coculturing diverse clonal populations prevents the early-stage neoplastic progression that occurs in the separate ...
Mixing clones before the 1° assay suppresses the progression from light to dense focus formation that occurs in separate clones in the 2° and 3° assays.
A 1° assay of each clone was done after the 37th LDP (fourth series) and was followed by mixing the clones.
in the 2° assay and of clone 2B in the 3° and 4° assays.
www.pnas.org /cgi/content/full/97/1/174   (3251 words)

  
 [No title]
If the tag is~1, the address gives the serial number of the destination vertex; all vertices are given a serial number, starting with~1.
If this vertex has clones $v_a$, $v_{a+1}$, \dots,~$v_b$, the number of paths from the source to each clone is accumulated in $b-a+1$ consecutive entries of ctable.
Of course this serial number is not the same as the hexadecimal number of the corresponding pattern in {\mc POLYENUM}; we haven't been told that number.
www-cs-faculty.stanford.edu /~knuth/programs/dagenum.w   (1721 words)

  
 Drastic Fitness Loss in Human Immunodeficiency Virus Type 1 upon Serial Bottleneck Events -- Yuste et al. 73 (4): 2745 ...
Mixed infections with clones J1 and A1 were carried out with decreasing (from 100 to 0%) and increasing (from 0 to 100%) proportions of the two viruses.
observed with clones A1 and G1 at passage 8 and with clones E1 and H1 at passage 14.
Clone J1 was used as a reference, and consequently its slope is zero.
jvi.asm.org /cgi/content/full/73/4/2745   (5058 words)

  
 Serial TCR Engagement and Down-Modulation by Peptide:MHC Molecule Ligands: Relationship to the Quality of Individual ...
B and C, T cell clones A.E7 (B) and 3C6 (C) were cultured with P13.9 and various concentration of PCC88–104 for 5 h.
T cell clones A.E7 and 3C6 were cultured with P13.9 at a ratio of 1:2.
CTL clones is determined by the frequency of serial TCR engagement.
www.jimmunol.org /cgi/content/full/162/4/2073   (6756 words)

  
 Gentoo Linux Documentation -- OpenBoot PROM (OBP) Reference
The Stop key is the top left key on the extra block of keys on the left hand side of the keyboards (on really old systems it might be marked L1).
Additional diagnostic information will be provided over the serial console when the machine boots.
Note that some tests (for example, serial ports) assume that an external loopback connector is attached.
www.gentoo.org /doc/en/gentoo-sparc-obpreference.xml   (1285 words)

  
 Hypersensitivity Reactions to Carbamazepine: Characterization of the Specificity, Phenotype, and Cytokine Profile of ...
All of the clones responded to CBZ in a dose-dependent manner (Fig.
With the exception of clone CBZ 25, the capacity of dihydro CBZ and CBZ-10,11-epoxide to stimulate proliferation was significantly
Tennis P and Stern RS (1997) Risk of serious cutaneous disorders after initiation of use of phenytoin, carbamazepine, or sodium valproate: a record linkage study.
molpharm.aspetjournals.org /cgi/content/full/63/3/732   (4816 words)

  
 Evolutionarily Related Sindbis-Like Plant Viruses Maintain Different Levels of Population Diversity in a Common Host -- ...
There are a number of possible scenarios that could affect diversity levels of RNA viruses in serial passage experiments.
Distribution of accumulated mutations observed in clones derived from CMV populations in N.
Distribution of accumulated mutations observed in clones derived from TMV populations in N.
jvi.asm.org /cgi/content/full/74/7/3130   (3877 words)

  
 Decreased Binding of Peptides-MHC Class I (pMHC) Multimeric Complexes to CD8 Affects Their Binding Avidity for the TCR ...
Clone NM55, specific for the influenza matrix protein derived peptide Flu-MA, was used as an internal control.
The activity of peptide variants relative to that of the parental peptide was previously assessed (22) and is shown.
T cell clones was assessed in a CTL assay at the E:T ratio of 10:1 for 4 h at 37°C in the presence of serial dilutions of the indicated multimers.
www.jimmunol.org /cgi/pmidlookup?view=full&pmid=12734357   (4484 words)

  
 EMBEDDED SYSTEMS   (Site not responding. Last check: 2007-10-19)
Most clones will generate an int18h instruction if the boot from the floppy drive was unsuccessful (when the enter key is pressed, some may require more than one enter key press!).
This is due because most clones generate an int19h instruction to boot from floppy, and when this is unsuccessful, the type int18h instruction is executed, forcing a jump to F600:0000
Lifting the lid off the clone should reveal the ROM BIOS chip, and about four or five empty sockets (latest clones only have one empty socket, which takes up the memory space of the four or five empty ones on earlier machines).
www.ibilce.unesp.br /courseware/embedded/pc.htm   (2948 words)

  
 BCHM3001/3901_AMB_2   (Site not responding. Last check: 2007-10-19)
In shotgun sequencing the idea is to sequence millions of overlapping clones and then, once you have those sequences, to reassemble the genome sequence from the overlapping sequences.
This is really a question of probability, but it means that you have to sequence many more clones than you would if to fully sequence the genome once, because you have to have lots of overlapping pieces of sequence and you have to increase your chances of sequencing everything.
CONTIGS are regions of contiguous (very similar meaning to continuous, but implies positioned adjacent to one another) DNA of known sequence, these have been automatically assembled from multiple sequence reads from clones that have overlapping sequence.
www.biochem.usyd.edu.au /matthews/lect_2.htm   (1222 words)

  
 The Clones of Sax Rohmer's Dr. Fu Manchu   (Site not responding. Last check: 2007-10-19)
Opium's plans are foiled (usually unwittingly) by his sworn enemy, a vain and silly TV anchor, whose fiancé is usually abducted in each episode (in the end of the first series, she apparently drops her macho boyfriend and strikes a friendship with Opium's female sidekick).
The series is designed with tongue firmly in cheek, in a "clear line" style reminiscent of Tin-tin or The Phantom.
By the midway point of the series, the production of the Destroyer books had been turned over to ghost-writers, of whom by far the most prolific was Will Murray.
www.njedge.net /~knapp/clones.htm   (5713 words)

  
 Jedi Council Forums - Serial movie making died long ago for a reason...............
Jedi Council Forums » Star Wars Films » Attack of the Clones » Serial movie making died long ago for a reason...............
The reason we disagree about whether serials are an influence, is the same reason why some people are disappointed with the effects in the PT, which is the same reason why some people think the story is lacking or two complicated.
Lucas said at the beginning he loved those old serials when they had terrible production values and he felt if the same type of movies from that era were done first class, they'd be successful.
boards.theforce.net /Attack_of_the_Clones/b10001/9976580/p5   (2497 words)

  
 Amelioration of the Cost of Conjugative Plasmid Carriage in Eschericha coli K12 -- Dahlberg and Chao 165 (4): 1641 -- ...
One random clone from each population was subjected to curing, leading to a plasmid-free evolved host (b
Curing was accomplished through infection by an incompatible replicon: pKG339 for R1-carrying clones and pFF1ts97 for RP4-carrying clones (see main text for details).
The plasmids from the evolved clones were transferred into the
www.genetics.org /cgi/content/full/165/4/1641   (4250 words)

  
 George Lucas Speaks Serialsly - www.ezboard.com
On the new Star Wars Part II Attack of the Clones dvd, there is some audio commentary that references serials.
"...the whole series of these films is based on a 30s genre of a serial...the acting style is very much from the 30s...
I haven't seen CLONES, but I do know this: if Lucas was emulating FLASH GORDON and BUCK ROGERS with his earlier STAR WARS pictures, by PHANTOM MENACE he was apparently trying to pay homage to THE CLUTCHING HAND and THE BLACK COIN....
p078.ezboard.com /fmovieserialmessageboardsfrm30.showMessage?topicID=16.topic   (668 words)

  
 Bonsi Mom ? by OOgleBird   (Site not responding. Last check: 2007-10-19)
The approach of taking serial clones is to me very appealing...
SO as plants are being moved to flowering a couple clones are taken and forever the cycle continues...
Now revegging a plant to take cuttings as a means of saving a great plant is a good idea....
www.overgrow.com /edge/showpost/p-2089912.html   (200 words)

  
 City of Hope :: Comprehensive Cancer Center
Greenberg, P.D., Nelson, B., Gilbert, M., Sing, A., Yee, C., Jensen, M., and Riddell, S.R.: Genetic modification of T cell clones to improve safety and efficacy of adoptive T cell therapy.
The long-term goals of this project are to develop antigen-specific adoptive therapy for B-cell malignancies employing T-cells genetically modified for re-directed tumor recognition for targeting post-hematopoeitic stem cell transplant minimal residual disease.
The major goals of this project are to develop adoptive T-cell therapy with CD20-specific effector cells for patients with relapsed/refractory CD20+ follicular lymphoma.
www.cityofhope.org /ccc/bio/mjensen.asp   (1371 words)

  
 ASTALAVISTA.BOX.SK   (Site not responding. Last check: 2007-10-19)
clone developed for use with the Linux Router Project.
Super, a sudo clone, v3.18 local root format string exploit.
beace - socks 5 irc clone loader that loads a variable number of clones through different socks servers onto different irc servers, then executes a predefined script.
astalavista.box.sk /cgi-bin/robot?srch=clone   (1375 words)

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