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Blood plasma - Biocrawler   (Site not responding. Last check: 2007-10-12) Serum is the same as blood plasma except that clotting factors (such as fibrin) have been removed. For this purpose, a serum-separating tube (SST) can be used which contains an inert catalyst (such as glass beads or powder) to facilitate clotting as well as a portion of gel with a density designed to sit between the liquid and cellular layers in the tube after centrifugation, making separation more convenient. Serum is preferred for many tests as the anticoagulants in plasma can sometimes interfere with the results. www.biocrawler.com /encyclopedia/Serum   (476 words)

Serum-separating tube - Wikipedia, the free encyclopedia They are used in medical clinical chemistry tests requiring blood serum, and contains a special gel that separates blood cells from serum, as well as particles to cause blood to clot quickly. These tubes cannot be used for therapeutic drug levels because the drug will diffuse from the serum into the gel, causing a reduction in measured drug level. SST stoppers are either gold or red-gray (they are sometimes called "marble-top tubes" for this reason). en.wikipedia.org /wiki/Serum-separating_tube   (139 words)

Blood plasma - Wikipedia, the free encyclopedia Serum is the same as blood plasma except that clotting factors (such as fibrin) have been removed. For this purpose, a serum-separating tube (SST) can be used which contains an inert catalyst (such as glass beads or powder) to facilitate clotting as well as a portion of gel with a density designed to sit between the liquid and cellular layers in the tube after centrifugation, making separation more convenient. Serum is preferred for many tests as the anticoagulants in plasma can sometimes interfere with the results. en.wikipedia.org /wiki/Serum   (728 words)

s010413a - Iron-Deficiency Anemia - Serum Ferritin Diagnosis Serum transferrin receptor and its ratio to serum ferritin in the diagnosis of iron deficiency. Serum ferritin (SF) was determined in 1978 in frozen sera using the Ramco IRMA and, in 1992, samples were re-analysed using a RIA calibrated with the International Standard 80/602 for SF determination. Nonheme iron and ferritin in the bone marrow and serum ferritin was decreased in patients with iron deficiency anaemia. www.emory.edu /WHSCL/grady/amreport/litsrch00/s010413a.html   (7138 words)

Apparatus and process for producing vacuum blood collecting tubes - Patent 5129213 The vacuum blood collecting tube 2 as completed has a known blood coagulant a sprayed onto the inner surface of the tubular container 1, a known serum separating agent b injected into the bottom portion 1b, a stopper 3 applied to the opening portion 1a and a vacuum in its interior. A serum separating agent tank 65 is disposed above the front and rear lift tables 61 and has fixed to its bottom a pair of front and rear nozzle cases 67, with a change-over valve unit 66 interposed therebetween. The device H is adapted to evacuate the tubular container 1 to a vacuum after the serum separating agent b has been injected into the container with the blood coagulant a applied thereto, and to close the opening portion 1a of the container with the stopper 3. www.freepatentsonline.com /5129213.html   (7639 words)

Complement C1 Esterase Inhibitor, Serum C1 esterase inhibitor is decreased in hereditary angioneurotic edema; decrease may be functional or quantitative In addition to decreased C1 esterase inhibitor in the serum of patients with hereditary angioneurotic edema, a unique polypeptide kinin is increased in plasma from C1 esterase inhibitor deficient patient during attacks of swelling. Consequently, measurement of serum C4 is an often used test. www.labcorp.com /datasets/labcorp/html/chapter/mono/sc003000.htm   (319 words)

Phlebotomoy continuing education study material for RNs, LPNs from Florida nursing educators FLC Nursing   (Site not responding. Last check: 2007-10-12) Engage the vacuum tube and be sure to hold the needle/hub steady or you may lose the vein or cause pain or injury to the patient. If multiple tubes are to be drawn, mix each tube when you remove it from the needle/hub assembly; engage the next tube, etc., until you have drawn all the required tubes. Tubes too small to be labeled, such as hematocrit tubes, may be placed in a plain (red top) tube and the tube can be labeled. www.nursingclasses.com /phlebotomy-nursing-ceu-module.html   (6527 words)

Brazilian Journal of Infectious Diseases - Comparison of four extraction methods to detect hepatitis A virus RNA in ...   (Site not responding. Last check: 2007-10-12) Serum samples were also tested for hepatitis B surface antigen (HBsAg) and for IgM antibodies directed against the hepatitis B virus core protein (IgM anti-HBc), using Hepanostika HBsAg Uni-form II and HBc IgM kits (Organon Teknika, Boxtel, The Netherlands), respectively, in order to check for other hepatitis etiologies. The tube was centrifuged at 8,000g for 2 min to sediment the silica particles, and the supernatant containing the RNA was used for cDNA synthesis and enzymatic DNA amplification. The serum and stool samples from all the patients were tested for HAV RNA with four extraction methods (Table 2). www.scielo.br /scielo.php?script=sci_arttext&pid=S1413-86702003000200007&lng=es&nrm=iso   (2922 words)

RML | Collection Tube Description Royal Blue topped tubes are special acid washed tubes for the collection of trace elements or heavy metals and cellular immunology studies. Contains clot activator and inert gel for separating serum, which acts as a barrier between cells and serum after centrifugation. The use of a transfer pipette is the required method, please DO NOT tilt the original tube when separating the serum into an aliquot tube. www.rmlonline.com /general.asp?id=sp36   (372 words)

Welcome to AJC! | ajc.com Serum hemoglobin is a test that measures the level of free (that is, outside red blood cells) hemoglobin in the blood. The area is cleansed with antiseptic and punctured with a sharp needle or a lancet. These are bound to a serum protein called haptoglobin and taken up by the liver. www.ajc.com /health/healthfd/shared/health/adam/ency/article/003677.html   (624 words)

Centrifuge tube assembly - US Patent 6835353   (Site not responding. Last check: 2007-10-12) It is a further object of this invention to provide a centrifuge tube assembly that permits chemicals and bodily fluids to be separated and individually aspirated with minimal cross-contamination. This invention features a centrifuge tube assembly having an elongate tubular receptacle and a cap that is removably or permanently engaged with an open upper end of the tubular receptacle. 1 a centrifuge tube assembly 10 that includes a tubular or cylindrical receptacle 12 and a cap 14 that is releasably engaged with an open upper end of receptacle 12. www.patentstorm.us /patents/6835353.html   (4354 words)

RIA Methods This blue light is detected and measured by photomultiplier tubes in a similar manner, and the pulse height represents the energy of the beta. After the free liquid fraction, or supernatant, of the assay tube is decanted and thrown away, the assay tube and the precipitate or pellet are counted in the gamma counter. Separate accuracy and parallelism tests are done for each medium assayed, even if the various media come from the same species. ink.primate.wisc.edu /~assay/riameth.html   (2360 words)

Carle Lab visit   (Site not responding. Last check: 2007-10-12) And then the blood is placed in a serum separating tube, which is "one of the most important tubes in chemistry" made by placing a gel with a density intermediate to red blood cells and blood serum, so that the two separate and keep there distinct characteristics. The tubes then went down to a site where a series of tests were down on the sample, all injectors had either air washers or water washers attached to keep contaminate free all the time. A sample was taken from the tube of the conveyor belt and placed in a cuvette then the 3 components were added, the wheel did a lot of spinning all the time and a photometer took a reading on each cuvette at each revolution, each reading took aproximately.5s and stored in a computer host. www.scs.uiuc.edu /~acs/minutes/031204.html   (601 words)

VACUTER The Gel and Clot Activator Tube is used to collect blood for clinical biochemistry and immunology. This tube contains a barrier polyer - gel which is present at the bottom, which forms a barrier separating serum from fibrin and cells during centrifugation. Serum may be aspirated directly from the collection tube, eliminating the need for transfer to another container. www.suru.com /sst.htm   (168 words)

ipedia.com: Serum-separating tube Article   (Site not responding. Last check: 2007-10-12) These tubes cannot be used for therapeutic drug levels because the drug will diffuse from the serum into the gel, causing a reduction in measured drug level. SST stoppers are either gold or red-gray (they are sometimes called "marble-top tubes" for this reason). The gel in SST II tubes (which appears slightly less opaque) is supposed to have less effect on drug levels in serum. www.ipedia.com /serum_separating_tube.html   (180 words)

Should I Use Serum or Plasma for my HumanMAP Study? Serum, heparin-plasma, citrated-plasma and EDTA-plasma were collected during the same blood draw using standard clinical tubes from 12 healthy volunteers. In addition, serum to plasma ratios for the cytokines, chemokines, growth factors, and other analytes were developed as important, useful tools for investigators planning MAP studies. One tube of each of the resulting 48 samples were thawed at room temperature, vortexed, spun at 13,000 x g for 5 minutes for clarification and 25 uL was removed for MAP antigen analysis into a master microtiter plate. www.rulesbasedmedicine.com /serum_or_plasma.asp   (1870 words)

Exercise 4.7 - Separation of LDH Isozymes from Serum Assemble the upper and lower chambers, ensuring that the lower portions of the gel tubes are immersed in the lower bath and that all trapped air bubbles are removed from the ends of the tubes. While holding the gel tube with one hand, carefully insert the needle at the sample application end of the tube, between the inside wall of the tube and the gel. Serum lactic dehydrogenase (LDH) is an ideal enzyme for the analysis of isozymes, and is also a model system for electrophoretic analysis. homepages.gac.edu /~cellab/chpts/chpt4/ex4-7.html   (1694 words)

Serum preparator (US4443345) The invention is used in conjunction with the centrifugal separation of serum from blood for filtering fibrin and other particulates from the separated serum and for partitioning the serum filtrate from unfiltered blood. The density of the serum preparator causes it to seek an isopycnic equilibrium stop position near the interface of the separated serous and cellular components. At the isopycnic stop position, the serum preparator forms an impermeable barrier to protect the serum filtrate from contamination by the cellular component during storage and decanting. www.delphion.com /details?pn10=US04443345   (556 words)

Texas Department of State Health Services - Guidelines for Specimen Collection and Submission   (Site not responding. Last check: 2007-10-12) Serum samples that are to be tested fresh may be stored for up to two hours at 2-8°C in the presence of clots. Serum may be separated from the clot by centrifugation and transferred to a sterile tube with a screw-cap (make sure that the seal is tight to prevent leakage). Serum separated from the clot may be stored at 2-8°C up to 48 hours. www.dshs.state.tx.us /lab/MRS_specimens.shtm   (2078 words)

Trace Element Analysis Lab at Dartmouth Both can be used alone to separate components of a mixture, but each is often a component of a “hyphenated” technique with one of the ICP-MS instruments. The simplest form of chromatography is separating a mixture on a piece of filter paper. Since the anions in the sample are attracted to the positively charged ammonium groups in the column, the higher the affinity of the ion the longer it takes for that ion to travel through the column. www.dartmouth.edu /%7Ecehs/indexTE.html   (1354 words)

11. Serology Serum known to contain antibodies to Newcastle disease virus is used to confirm the presence of Newcastle disease virus in a test sample of allantoic fluid. Serum samples are collected for testing for the presence of antibodies to Newcastle disease virus. Once the HI titre of the international standard serum and the national standard serum have been established, prepare a series of two-fold dilutions of the serum that are spread around the endpoint used to establish the HI titres. www.fao.org /docrep/005/ac802e/ac802e0e.htm   (3177 words)

Fructosamine Measurement in Diabetic Dogs and Cats If using a clot tube, the blood should be withdrawn from the patient into the collection tube, allowed to coagulate, and then centrifuged to separate the serum. The entire blood sample may then be refrigerated until submitted to the laboratory, and separating the serum from the blood cells is unnecessary. Because hyperthyroidism decreases fructosamine concentration, serum fructosamine levels should be used with caution in the diagnosis and monitoring of diabetes mellitus in cats with underlying hyperthyroidism. www.vet.uga.edu /vpp/clerk/bates/index.php   (2838 words)

The Individualist: Optimum Proportions Determination (Flocculation Optima) The diluted antigen and saline are mixed first, then the diluted serum added to one tube at a time, beginning at the right; the time each mixture is placed in the water bath is recorded. From the antigen and antibody dilutions present in these tubes, it may be calculated what would be a suitable dilution of antigen and antibody for the "fine test." The serum dilution should be such that flocculation at the optimum may be expected to take place in 5 to 10 minutes. The decisive factors appear to be the number of polar groups of the antigen (hapten) left free, and the distance separating the different reactive groups, which determines the amount of steric hindrance exerted by one antibody molecule on another. www.dadamo.com /wiki/wiki.pl?action=browse;id=Optimum_Proportions_Determination_(Flocculation_Optima);revision=3   (918 words)

SST   (Site not responding. Last check: 2007-10-12) SST Ramps Foundry Capacity to Meet Demand for Serial Flash in 2005; SST's Serial Flash Capacity is Expected to Exceed 15... SST And SANYO Pool Resources to Expand Flash Technology Partnership into China; SST and SANYO Celebrate 10-Year Partners... SST Reports Third Quarter 2003 Financial Results; Unit Shipments Up 24 Percent Sequentially. hallencyclopedia.com /SST   (391 words)

Microvolume Blood-Sampling Device with Low Hemolysis and High Consistent Yield of Serum Components -- Tanaka et al. 47 ... Principles of blood separation and serum recovery by the new and conventional membrane methods. Stability of serum components at room temperature after 100 µL of fresh, healthy human blood was dropped on each of sucrose-treated and untreated membranes with an A/B of 0.5 (n = 41). Blood cell separation occurs automatically in the interior, and serum (or plasma) fills the downstream portion across the groove. www.clinchem.org /cgi/content/full/47/10/1829   (3409 words)

ACVC 2001 - Small Mammal Clinical Pathology: The Basics If the plasma is not separated from the blood cells and if it is sent to the lab unspun, at least three analytes of the chemistry panel will be affected, blood glucose, potassium and phosphorus. There are some drawbacks when separating the plasma from the cells in the hospital. Finally, if the same tube is used to evaluate the CBC and the biochemistry profile, then the tube cannot be spun as this will render the cells useless for the CBC. www.vin.com /VINDBPub/SearchPB/Proceedings/PR05000/PR00400.htm   (1549 words)

newsletter36   (Site not responding. Last check: 2007-10-12) However for the other 99.9% of the time simplest solution is to separate the serum or plasma from the red sells or clot as soon as possible after collection. In addition to separating serum samples it is also helpful to remove heparin plasma and oxalate fluoride plasma before submission but make sure you label the tubes clearly. Serum Gel tubes and plain tubes for separated plasma are available on request from client services, call 01253 899215. www.nwlabs.co.uk /newsletter36.htm   (1400 words)

Chemical & Engineering News: Cover Story - Monolithic Chromatography When a sample solution is injected into the column, the effects of diffusion, which are linked to differences between analyte concentrations in the void spaces and the interiors of the particles, cause the analyte molecules to be transferred back and forth between the two regions. This process limits the column's ability to thoroughly separate analytes with similar properties and can lead to slow separations, especially for large molecules such as proteins and synthetic polymers, which tend to move sluggishly due to their small diffusion coefficients. To aid in separating mixtures with thousands of components, as encountered, for example, in proteomics research, manufacturers of HPLC equipment have moved toward columns with diameters of a couple of micrometers or less. pubs.acs.org /cen/coverstory/84/8450cover.html   (2087 words)

Centrifuging Blood Collection Systems Our preference is that you use a plastic SST Vacutainer® type (serum separation tube) for most tests requiring a serum specimen. Serum separator collection tubes contain a gel that separates the clot from the serum in whole blood specimens. Serum separator tubes are recommended for most testing, but please check individual specimen requirements for restrictions. www.legacyhealth.org /body.cfm/documents/Misc/body.cfm?id=815   (392 words)

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