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Topic: Shotgun sequencing

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Bioinformatics

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	Genome Sequencing; DNA, PCR, & MicroRNA Analysis; De Novo Assembly - 454 Life Sciences™
		With 454 Sequencing technology each molecule/ amplicon within the mixture is sequenced individually allowing for the identification of rare variants and the assignment of haplotype information over the full sequenced length.
		cDNA sequencing for de novo assembly of the transcriptome or mapping to a scaffold genome for gene discovery.
		De novo sequencing and assembly of your individual BACs or pool BACs to map it to a particular region of the genome.
	www.454.com /sequencing-services/services.asp (311 words)

	12.21.2006 - Shotgun sequencing finds nanoorganisms
		What made Baker's find possible was shotgun sequencing, a technique developed and made famous by Celera Corp., which used it to sequence the human genome in record time.
		In 2004, Banfield collaborated with the Department of Energy's Joint Genome Institute to shotgun sequence a drop of the slime.
		This type of sequencing involves homogenizing the organisms in the sample, isolating the combined DNA and breaking it into lots of random strands.
	www.berkeley.edu /news/media/releases/2006/12/21_microbes.shtml (1049 words)

	Sequencing Medicago truncatula at the University of Oklahoma
		Sequencing Medicago truncatula at the University of Oklahoma
		Sequencing Medicago truncatula Cultivar Jemalong A17 at the University of Oklahoma
		Chromosome 3 is being sequenced at Sanger/JIC and chromosome 5 is being sequenced at Genoscope/INRA as part of the European Consortium.
	www.genome.ou.edu /medicago.html (1306 words)

	Ask A Scientist - Shotgun cloning method
		The whole-genome shotgun sequencing method has been made feasible by recent advances in sequencing technology and in computational methods designed to assemble contiguous stretches of DNA from a large pool of overlapping shorter fragments.
		The shotgun approach uses random cloning of relatively small pieces of genomic DNA and subsequent sequencing of many clones to provide 10- to 12-fold coverage of the whole genome length.
		To use the whole-genome shotgun sequencing method, Celera had to build the most powerful nongovernment supercomputing facility in the world that is specifically dedicated to sequence analysis.
	www.hhmi.org /cgi-bin/askascientist/highlight.pl?kw=&file=answers/genetics/ans_021.html (797 words)

	The Dictyostelium Sequencing Project
		Shotgun sequencing of fragments in the size range of 100 to 1,000 kb would then be relatively straight-forward.
		The judgement of the genome sequencing community is that it should be possible to use a shotgun approach to sequence many genomes in the near future.
		End sequencing of each insert and complete sequencing of a small subset of the inserts will allow many of the clones to be positioned relative to each other on the basis of shared sequence.
	dictybase.org /genomeseq.htm (2554 words)

	SeqWright's Shotgun Sequencing Service Through Fisher Scientific
		This vector is an excellent choice for construction of libraries for gap closure and finishing procedures in shotgun sequencing projects.
		Sequencing of random clones from a shotgun library is not generally enough to give full length double stranded coverage.
		The full length shotgun sequencing service will provide a guaranteed full length double-stranded sequence with an accuracy of 99.99%, suitable for publication purposes.
	www.fishersequencing.com /OldStyle/ShotgunSequencing.htm (469 words)

	cDNA Sequencing Program
		The Baylor College of Medicine Human Genome Sequencing Center's (Baylor HGSC) Concatenation cDNA Sequencing (CCS) program is supported by funding from the Department of Energy (DOE) and the National Institute of Health (NIH).
		The goal of the HTD is to provide a clone-based platform that represents as many of the transcripts that have been sequenced as possible.
		Primary cancer tissues (not cell lines) are used to avoid the sequencing of expressed genes that may be related to growth in culture, but not to the histology of the tumor.
	www.hgsc.bcm.tmc.edu /projects/cdna (477 words)

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