| | Millipore - Technical Library - MultiScreen Solubility Filter Plate: Quantitative method to determine drug aqueous ... |
 | | The MultiScreen Solubility filter plate was developed and is QC tested for consistent filtration flow-time (using standard vacuum), low aqueous extractable compounds, high sample filtrate recovery, and its ability to incubate samples as required to perform the solubility assay. |
 | | The final drug concentration in the filtrate is determined by dividing the absorbance by the slope of the line from the calibration curve and multiplying by a factor of 1.25 to account for dilution with acetonitrile prior to obtaining the absorbance spectrum. |
 | | For both compounds, a solubility in excess of 350 µM was determined using the MultiScreen Solubility filter plate while the shake-flask method produced values of less than 100 µM. For these two compounds, the presence of 5% DMSO significantly elevates their solubility. |
| www.millipore.com /publications.nsf/docs/an1730en00 (2626 words) |