
	Where results make sense

Topic: Southern blot

Related Topics

Northern blot

Polymerase chain reaction

Western blot

Restriction fragment length polymorphism

Molecular biology

Genetics

Nitrocellulose

Back end

Ziggy Stardust

UNIDO

Voyager program

Bacterium

Datuk

Chandler, Arizona

Caguax

In the News (Tue 14 Feb 12)

EXECUTIVE POWER

Iran

ANALYSIS

Pakistan

Family compact

	Southern Blot
		The Southern blot, named for its inventor British biologist Edwin Southern, is a method for marking specific DNA sequences and detecting the presence of a specific gene.
		Blots are techniques for transferring DNA and RNA proteins onto a carrier so they can be separated, and often follows the use of a gel electrophoresis.
		The Southern blot is used for transferring DNA.
	www.iscid.org /encyclopedia/Southern_Blot (245 words)

	Southern blot - Wikipedia, the free encyclopedia
		A Southern blot is a method in molecular biology of enhancing the result of an agarose gel electrophoresis by marking specific DNA sequences.
		This caused other blot methods to be named similarly as plays on Southern's name (for example, Western blot, Northern blot, Southwestern blot).
		If DNA is larger than 15 kb, prior to blotting, the gel may be treated with a weak acid, such as dilute HCl, which acts to depurinate the DNA fragments.
	en.wikipedia.org /wiki/Southern_blot (421 words)

	Solving Problems (Site not responding. Last check: 2007-10-15)
		Blot A shows that exon 1 is present in both male and female mRNA for this gene.
		Blot C shows that exon 2 is present in the female mRNA but not in the male mRNA - note that this does not conflict with Blot B's result.
		Blot D shows that exon 3 is present in both male and female mRNA for this gene.
	web.mit.edu /esgbio/www/rdna/solvingblots.html (1927 words)

	Southern blotting
		Southern blotting was named after Edward M. Southern who developed this procedure at Edinburgh University in the 1970s.
		Southern blotting is designed to locate a particular sequence of DNA within a complex mixture.
		For example, Southern Blotting could be used to locate a particular gene within an entire genome.
	lifesciences.asu.edu /resources/mamajis/southern/southern.html (719 words)

	Blot, Southern definition - Medical Dictionary definitions of popular medical terms
		Blot, Southern: A test commonly used in molecular biology and genetics, the purpose of the test being to check for a match between DNA molecules.
		In the Southern blot technique, DNA fragments are separated by a method called agarose gel electrophoresis, transferred (blotted) onto membrane filters, and hybridized with complementary radiolabeled probes.
		Lest all of this sound esoteric, note that in the television series "The X Files" a Southern blot was done (in this case, to learn if some alien virus genome had been integrated into a person's genome).
	www.medterms.com /script/main/art.asp?articlekey=2496 (182 words)

	Southerns, Northerns, Westerns, & Cloning: Molecular Searching Techniques (Site not responding. Last check: 2007-10-15)
		Note: In a Southern Blot, the DNA molecules in the gel are double-stranded, so they must be made single stranded in order for the probe to hybridize to them.
		Although Southern blotting involves DNA-DNA hybridization, it is not a useful procedure for cloning a gene.
		If we were to cut human DNA with a restriction enzyme and run it on a Southern blot probed with a clone of the actin gene from an other organism, we could construct a restriction map of the human actin gene.
	web.mit.edu /esgbio/www/rdna/rdna.html (3119 words)

	southern blot
		If the gel is smaller than 11 X 14, use parafilm to mask the edges of the blotter papers and the membrane underneath the gel to prevent the blotting solution from by-passing the gel.
		Discard blotting papers in the regular trash, and the gel in the ethidium waste container.
		When the cross-linking is complete, allow the blot to air dry on the bench for about 1 hour, then seal it in a heat-seal bag until use.
	www.ca.uky.edu /agcollege/plantpathology/Vaillanc/southbl.htm (1540 words)

	Southern Blot Protocol
		Disassemble the gel/membrane sandwich, and then slightly air dry the blot for approximately 30 seconds to dampness.
		UV crosslink the blot (we use a Spectrolinker XL-1500 UV crosslinker on the optimal setting).
		Wash the blot twice for 30 minutes at 50-60°C with 0.1X SSC/0.1% SDS (55°C is normally sufficient; this may vary from lot-to-lot of membrane).
	www.fda.gov /cder/dapr/tgacprot.htm (1015 words)

	[No title] (Site not responding. Last check: 2007-10-15)
		The Southern Blot is one way to analyze the genetic patterns which appear in a person's DNA.
		The gel with the size-fractionated DNA is applied to a sheet of nitrocellulose paper, and then baked to permanently attach the DNA to the sheet.
		If an X-ray is taken of the Southern Blot after a radioactive probe has been allowed to bond with the denatured DNA on the paper, only the areas where the radioactive probe binds [red] will show up on the film.
	protist.biology.washington.edu /fingerprint/blot.html (349 words)

	Normalization of muscle plasmid uptake by Southern blot: application to SERCA1 promoter analysis -- Mitchell-Felton and ...
		All lanes are from 1 blot where soleus muscle DNA was loaded in top tier and standards were loaded in bottom tier.
		Whole muscle luciferase activity (top) was normalized (bottom) by either Southern blot (A) or coinjection at a 1:50 ratio with pRL-CMV (B), a 1:20 ratio with pRL-CMV (C), a 1:10 ratio with pRL-TK (D), or a 1:3 ratio with pRL-SV40 (E).
		For Southern blots, whole muscle luciferase activity is normalized by total plasmid copy number uptake.
	ajpcell.physiology.org /cgi/content/full/277/6/C1269 (3588 words)

	Lab 4: Southern blot and plaque lifts
		The Southern blot is the most common way to analyse restriction endonuclease-digested DNA to find specific sequences of interest.
		The transfer of the DNA to the membrane is done by sandwiching the membrane between the gel and a stack of absorbent paper.
		Plaque lifts are a technique similar to the Southern blot.
	www.sfu.ca /biology/courses/bisc431/lab4.htm (1846 words)

	Southern Blotting (Site not responding. Last check: 2007-10-15)
		Answer: The Southern blotting technique is a technique that enables specific sequences of DNA, or specific genes to be identified.
		This gel is taken and the DNA transferred onto nitrocellulose membrane by Southern blotting.
		The blot can now be hybridised with a variety of radiolabelled probes to identify the presence of certain genes, or to identify sequences that are specific to individuals - in other words DNA fingerprinting.
	www.zephyrus.co.uk /southernblottinganswer.html (398 words)

	Southern Blot
		Analys of Genomic DNA by Southern Hybridization (Southern Blot)
		Localization of particular sequences within genomic DNA is usually accomplished by the transfer techniques described by Southern (1975).
		Do not allow blot to dry at any time prior to removing the probe, as drying will cause the probe to bind irreversibly.
	www2.kumc.edu /soalab/LabLinks/protocols/southern.htm (1595 words)

	Southern Blot
		In this case there are three different full mutation sizes, 280, 430 and 920 repeats (sized by Southern blot analysis).
		This full mutation has 355 repeats (sized by Southern blot analysis).
		In this case the full mutation has 510 repeats (sized by Southern blot analysis) and the premutation has 84 repeats (sized by PCR analysis).
	www.fragilex.org /html/southern.htm (185 words)

	Southern blot - Glossary Entry - Genetics Home Reference
		The separation on an electrophoretic gel of sequences or fragments of genomic or complementary DNA, partially digested by endonucleases, The fragments are then 'blotted' onto a membrane and allowed to hybridize with a specific, labeled probe in order to detect which bands contain the fragment or gene of interest.
		Southern blot is particularly useful in detecting large gene rearrangements/deletions and large trinucleotide repeat expansions.
		A technique used to identify and locate DNA sequences which are complementary to another piece of DNA called a probe.
	ghr.nlm.nih.gov /ghr/glossary/southernblot (112 words)

	Southern Blot (Site not responding. Last check: 2007-10-15)
		Blot with a Whatman 3MM filter paper to remove excess moisture.
		For nylon membranes only, place the membrane in Saran wrap, and UV treat for 2 to 5 minutes with the DNA side facing the UV light.
		Copyright 1993, 1996, and 1997 by Mark Barton Frank, Ph.D. Proper citation for data acquired from this document is: "Frank, M. Southern Blot Protocol.
	omrf.ouhsc.edu /~frank/SBLOT1.html (537 words)

	Southern Blot (Site not responding. Last check: 2007-10-15)
		The Southern blot is used to detect the presence of a particular bit of DNA in a sample.
		specific for the gene in question is incubated with the blot.
		The blot is washed to remove non-specifically bound probe and then a development step allows visualization of the DNA that is bound.
	www.escience.ws /b572/L22/south.html (254 words)

	Micrococcal nuclease−Southern blot assay - Nature Methods (Site not responding. Last check: 2007-10-15)
		To determine whether a DNA fragment of interest is within a nucleosome, the genomic DNA is subjected to Southern blot analysis.
		If a probe derived from the DNA fragment hybridizes to the ladder of nucleosomal bands, the fragment may indeed be assembled into nucleosomes.
		To determine nucleosome positioning, the purified genomic DNA must be cleaved with a restriction enzyme before gel electrophoresis and Southern blot analysis.
	www.nature.com /nmeth/journal/v2/n9/abs/nmeth0905-719.html (205 words)

	Protocol for Northern and Southern Blot with DIG Probes
		Detection of single-copy genes in DNA from transgenic plants by nonradioactive Southern blot analysis.
		Incubate the sealed blot at 37°C for 5 to 15 min to enhance the luminescent reaction.
		You can mark the positgions of ribosomal bands are with a pencil, and hold a dried blot behind you X-ray film to see if this corresponds to the sizes of your RNA markers, and also to make sure you haven't got non-specific hybridization to the ribosomal rRNA.
	www.bioinformatics.vg /Methods/dignorthernf.htm (2038 words)

	Forms for Oligonucleotide Synthesis, DNA Sequencing and Proteomics
		To be able to detect the transgenic founders by Dot blot or Southern blot, we will require 1 mg of purified probe in a concentration of 0.1 mg/ml.
		Also, we will need the hybridization and washing conditions to be used.
		If the investigator would require a Southern blot to detect the transgenic founders then a map should be included indicating the expected restriction enzymes to be used for digestion and the expected fragment sizes.
	www.cfgbiotech.com /forms/Mouse_Screening/Southern_Blot (198 words)

	Gel Blotting
		Gel blotting is a technique for visualizing a particular subset of macromolecules — proteins, or fragments of DNA or RNA — initially present in a complex mixture.
		The presence of the probe in a particular band is revealed by autoradiography.
		As a humorous extension of the term "Southern blot", these have been dubbed "Northern" and "Western" blots respectively.
	home.comcast.net /~john.kimball1/BiologyPages/G/GelBlotting.html (249 words)

	Blot southern - definition from Biology-Online.org
		dna fragments are separated by agarose gel electrophoresis, transferred (blotted) onto membrane filters, and hybridised with complementary radiolabelled probes.
		The aim is to detect specific base sequenceswith the probes.
		The southern blot is named after its inventor, the British biologist M.E. southern.
	www.biology-online.org /dictionary/Blot_southern (180 words)

	A3243G - Jargon - Southern Blot (Site not responding. Last check: 2007-10-15)
		A Southern Blot is a method of analysing a large piece of DNA by chopping it up into small pieces using chemicals and enzymes.
		The resulting pattern is dependent on a number factors, including the size of the DNA pieces.
		A radioactive binding agent is then applied and a "blot" is taken onto a separate sheet.
	www.a3243g.com /jargon_southern_blot.asp (165 words)

	UNIT 1: WESTERN BLOTTING
		Western blotting is a method for identifying a specific protein in a complex mixture and simultaneously determining its molecular weight.
		Tank transfer blotting wastes large volumes of buffers and is not very versatile since only a single buffer can be added to the tank.
		The composition of the buffers is as given in Table I. 2. Dry blotting pads large enough to completely surround the gel to be blotted are wetted with the desired buffer.
	www.med.unc.edu /wrkunits/3ctrpgm/pmbb/mbt/WESTERN.htm (11616 words)

	Molecular Biology Protocols - Southern Blot
		2) Rinse blot with 6x SSC and bake 2 hrs at 80¡C. 3) Prehybe blot for 1 hr at 42¡.
		11) Withdraw a small amount of prehybe fluid from blot, mix with probe and add back to blot.
		1) Wash blot for 20 minutes in primary wash buffer at 42¡C. Discard solution and wash 2 x 10 minutes at 42¡C. 2) Wash 2 x 5 minutes with 20x SSC at room temperature.
	userwww.service.emory.edu /~smkelly/southern.html (301 words)

	Soriano Lab - Southern Blot Analysis (Site not responding. Last check: 2007-10-15)
		Using gloves, cut two sheets of Whatman 3MM filter paper larger than the gel and two sheets of filter paper and one sheet of HYBOND-N
		nylon membrane (Amersham Pharmacia) to the size of the desired blot.
		Blot membrane dry and proceed to pre-hybridization (15 minutes to 1 hour) and hybridization.
	www.fhcrc.org /science/labs/soriano/protocols/southern.html (313 words)

	Cloning and Molecular Analysis of Genes
		The hybridization or binding of a clone to DNA or RNA provides important information regarding the structure and the expression of the gene.
		Southern hybridizations involve the binding of a radioactive probe to a DNA molecule that is immobilized on a membrane filter.
		After a series of washes the filter is used to expose a piece of X-ray film.
	www.cc.ndsu.nodak.edu /instruct/mcclean/plsc431/cloning/clone7.htm (414 words)

	Molecular biology troubleshooting forum / too small fragments in southern blot (Site not responding. Last check: 2007-10-15)
		i have a great problem with the size of southern blot fragments.
		but after blotting via vacu blot system of biometra and labelling the blots with cdp-star (amersham) a fragment of only 7500bp appears.
		I am doing southern hybridization to confirm the presence of my transgene in sweet potato transgenic lines.
	biowww.net /forum/read/5/1955/2122 (301 words)

Try your search on: Qwika (all wikis)