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	Southwestern blotting - Health Encyclopedia (Site not responding. Last check: 2007-10-20)
		SOUTHWESTERN BLOTTING SOUTHWESTERN BLOTTING is a method to detect DNA-binding proteins following their separation by gel electrophoresis and transfer onto a membrane.
		As the name implies, Southwestern blotting is a variation of the Western blotting technique.
		These data suggest that the positive broad spot detected by Southwestern blotting is a cluster of several different proteins.
	www.steadyhealth.com /encyclopedia/Southwestern_blotting (101 words)

	Southwestern blot - Wikipedia, the free encyclopedia
		Southwestern blotting, based along the lines of Southern blotting (which was created by Edwin Southern), involves characterizing proteins that bind to DNA.
		The proteins undergo gel electrophoresis and are subsequently transferred to nitrocellulose membranes similar to other types of blotting.
		For other types of blotting, see Northern blotting, Southern blotting, Western blotting, and future blots for analysis.
	en.wikipedia.org /wiki/Southwestern_blot (115 words)

	[Frontiers in Bioscience 2, c9-11, June 15, 1997]
		Southwestern blotting, first described by Bowen and colleagues (1), is a powerful technique for identifying and characterizing DNA-binding proteins by their ability to bind to specific oligonucleotide probes.
		The usefulness of this technique is hampered by its requirement for relatively large amounts of nuclear proteins (typically 50-100 mg), problems with protein degradation during isolation, and the difficulties in achieving efficient electrophoretic separation and transfer of a wide molecular size range of proteins.
		A number of modifications to the Southwestern blotting was done to increase the sensitivity of detection without excessive background and with the improved ease of room temperature hybridization.
	bioscience.org /1997/v2/c/handen/9-11.htm (1132 words)

	Purification of the pets Factor. A NUCLEAR PROTEIN THAT BINDS TO THE INDUCIBLE TG-RICH ELEMENT OF THE HUMAN ...
		In addition, using glycerol gradient centrifugation, Southwestern blotting, electrophoretic mobility shift assays employing purified protein eluted from a gel, and a new in solution UV cross-linking competitive assay, we show that the dominant protein binding to the pets site is 43 kDa in size.
		Southwestern blotting experiments show that in cultured human T cells, as well as bovine spleen nuclear extracts, the predominant protein binding specifically to the pets site is 43 kDa in molecular mass.
		In this report, we describe the purification of a 43-kDa cellular factor that binds to an element of the HIV-2 enhancer that we have previously shown to be functionally important in mediating transcriptional activation in response to T cell stimulation (8, 10, 11).
	www.jbc.org /cgi/content/full/271/32/19599 (6100 words)

	Southern blot - Wikipedia, the free encyclopedia
		A Southern blot is a method in molecular biology of enhancing the result of an agarose gel electrophoresis by marking specific DNA sequences.
		This caused other blot methods to be named similarly as plays on Southern's name (for example, Western blot, Northern blot, Southwestern blot).
		Prior to blotting, the gel may be treated with a weak acid, such as dilute HCl, which acts to depurinate the DNA fragments.
	en.wikipedia.org /wiki/Southern_blot (400 words)

	JIS: Tschinkel 2.12.2002
		For southwestern blotting, proteins were electroblotted onto nitrocellulose membranes (Schleicher and Schuell, www.s-and-s.com) for 90 minutes at 100 V in 25 mM Tris base, 192 mM glycine, 20% v/v methanol (pH 8.3).
		The blots were then washed with binding buffer three times (20 min each), air-dried and exposed to Kodak X-ray films (Baldridge and Fallon, 1996).
		Proteins were blotted onto nitrocellulose and used for detecting the protein with a commercial mouse c-JUN antibody (generated against the conserved DNA binding domain) obtained from Santa Cruz Biotechnology Inc. (www.scbt.com).
	www.insectscience.org /2.21 (5191 words)

	Transcription Factor YB-1 Mediates DNA Polymerase {alpha} Gene Expression -- En-Nia et al. 280 (9): 7702 -- Journal of ...
		B, Southwestern blotting with nuclear extracts from MCs probed with the sense DPA RE-1 strand demonstrated binding to several nuclear proteins with relative molecular masses of 26, 32, and 52 kDa.
		Southwestern blot analysis was performed as described (13) using
		C, Western blot analysis of nuclear extracts was performed for DPA protein, revealing up-regulation of DPA protein content 6 h post-addition of 10% serum (lane 3).
	www.jbc.org /cgi/content/full/280/9/7702 (5890 words)

	Dr. Straney: Abstracts
		Using a homologous in vitro transcription system, a transcriptional activator of PDA1 was shown to be present in mycelial extracts which shared the sequence specificity characteristic of the PRF, indicating function of the DNA-binding protein in transcriptional control.
		A 70 kDa protein was shown to be a DNA-binding component of PRF by three independent assays for DNA-binding proteins: southwestern blotting, UV-crosslinking and binding to immobilized DNA.
		Southwestern blotting has identified one component of this binding activity to be a ~35 kDa protein.
	www.biologie.uni-hamburg.de /b-online/ibc99/pbio100/research.htm (2088 words)

	Effects of Age on the Posttranscriptional Regulation of CCAAT/Enhancer Binding Protein alpha and CCAAT/Enhancer ...
		Southwestern blot analysis of liver nuclear proteins that bind to the APRE of the AGP-1 gene after LPS treatment.
		Southwestern blot analysis of the mouse liver polysomal proteins from young (A, 4 mo) and aged (B, 28 mo) old livers.
		Southwestern blot analysis was performed on the COS-1 nuclear extracts used for the immunoblot analyses (lanes 13-16).
	www.molbiolcell.org /cgi/content/full/9/6/1479 (7009 words)

	The PARP Link : Southwestern & Footprint
		Blot the proteins onto a nitrocellulose membrane by electrophoretic transfer using 1x Transfer buffer for 1 hr at 200 mA in the cold room
		Renature the proteins by incubation of the blot in 1x binding buffer for 30 mn at 20°C
		Place the blot under autoradiography for 1 hr at 4°C (if followed by footprint).
	parplink.u-strasbg.fr /protocols/tools/southwestern.html (351 words)

	Detection Methods
		If the expressed proteins or phenotypes of interest can be detected with fluorescent reagents, transfected cell microarrays are viewed and photographed with a fluorescent microscope or a laser scanner.
		A "Western blot" protocol was developed to detect phenotypes or expressed proteins after transfected cell microarrays are carefully transferred to a nitrocellulose membrane.
		far western and southwestern blotting) may also be work with our system, but we have not test this possibility.
	jura.wi.mit.edu /sabatini_public/reverse_transfection/detection.htm (1061 words)

	Characterization of a Sodium-Response Transcriptional Mechanism -- Ruiz-Opazo et al. 30 (2): 191 -- Hypertension
		A, Southwestern blot analysis of rat A10 nuclear extract (lanes 1 through 4) reveals binding of the human (H) [-798] 5' flanking probe to approximately 41-, 85-, and 95-kD nuclear DNA binding proteins (lanes 3 and 4).
		B, Competition Southwestern blot analysis using the identical experimental conditions as described above but adding unlabeled -798 probe in molar excesses of 10-fold to 100-fold to show specificity by competition.
		Shown are baseline condition (lanes 1 and 2), 10-fold molar excess unlabeled probe (lanes 3 and 4), and 100-fold molar excess unlabeled probe (lanes 5 and 6).
	hyper.ahajournals.org /cgi/content/full/30/2/191 (4682 words)

	Novel Nuclear Signaling Pathway Mediates Activation of Fibroblast Growth Factor-2 Gene by Type 1 and Type 2 Angiotensin ...
		(Bottom) Western blot analysis of FGFR1 with McAb6 in the extranuclear (cytoplasmic, C) fraction an in the total nuclear lysate (N) (30 µg of total nuclear or cytoplasmic proteins per lane).
		Nuclear accumulation of FGFR1 in stimulated BAMCs was confirmed by Western blot analysis of nuclear lysates with FGFR1 McAb6.
		Nuclear (N) and cytoplasmic (C) extracts were prepared 48 h later and analyzed by Western blot with polyclonal FGF-2 Ab.
	www.molbiolcell.org /cgi/content/full/12/2/449 (6971 words)

	Medical Dictionary: Southern blotting - WrongDiagnosis.com
		Southern blotting : method of identifying DNA by cDNA or RNA hybridization after electrophoresis and transfer to nitrocellulose or other fixative.
		Other terms that may be related to Southern blotting:
		The description of Southern blotting may also be used for the following terms:
	www.wrongdiagnosis.com /medical/southern_blotting.htm (198 words)

	Functional Domains of Yeast Plasmid-Encoded Rep Proteins -- Sengupta et al. 183 (7): 2306 -- The Journal of Bacteriology
		Southwestern blotting was performed as described by Mellor et al.
		Western blot analysis was used to confirm expression of the Rep fusion proteins in the two-hybrid host.
		Rep fusion proteins and their truncated derivatives, tested for interaction with Rep1, Rep2, or DNA by two-hybrid, baiting, and Southwestern assays, are shown as rectangles.
	jb.asm.org /cgi/content/full/183/7/2306 (6521 words)

	Glossary of Molecular Biology Terminology -- Kaushansky 2002 (1): 490 -- Hematology
		blotting is capable of detecting clonal populations that represent
		This modification of a Southern blot is used to detect specific
		The probe for Northern blotting must be antisense.
	www.asheducationbook.org /cgi/content/full/2002/1/490 (7166 words)

	Evaluation of global DNA hypomethylation in human colon cancer tissues by immunohistochemistry and image analysis -- ...
		Results obtained on dot blots are illustrated by fig 2.
		Blots: Example of results observed by Southwestern blotting with anti-5-MeCyd antibodies.
		The ratios r between the surface of this portion and the surface of the entire lane are indicative of the sensitivity of each sample to digestion.
	gut.bmjjournals.com /cgi/content/full/47/5/689 (2842 words)

	Transcription Factor Ets-1 Regulates Gelatinase A Gene Expression in Mesangial Cells -- Reisdorff et al. 13 (6): 1568 ...
		Nuclear (NE) and cytosolic extracts (CE) from rat MC were analyzed by Western blotting using two different anti–Ets-1 antibodies directed against epitopes within the protein C- (C275; panel A) and N-terminal regions (N276; panel B).
		With antibody C275, bands corresponding to 15, 33, 54, 65, and 97 kDa were detected in both subfractions (lanes 1 and 2 in panel A), whereas a control reaction performed in the absence of primary antibodies revealed no bands (lane 3 in panel A).
		Southwestern blotting was performed using a radiolabeled oligonucleotide encompassing the consensus Ets-1/PEA3–binding motif (C), yielding a similar band patterning as the one obtained with crossreactive antibody C275 with molecular sizes of 15, 33, 54, and 65 kDa, whereas the 97 kD band was absent.
	jasn.asnjournals.org /cgi/content/full/13/6/1568 (5888 words)

	In Azotobacter vinelandii, the E1 subunit of the pyruvate dehydrogenase complex binds fpr promoter region DNA and ...
		Lane a, Coomassie-stained gel; and lane b, Southwestern blot identifying fprBP as the upper band.
		Southwestern blotting was done with modification from Handen and Rosenberg (17) in 50 mM Tris·HCl (pH 7.5)/60 mM KCl/10%
		blots were used to determine which protein band bound specifically
	www.pnas.org /cgi/content/full/96/22/12389 (3090 words)

	Identification of a new cAMP response element-binding factor by southwestern blotting.
		Identification of a new cAMP response element-binding factor by southwestern blotting.
		Identification of a new cAMP response element-binding factor by southwestern blotting.This reverse effect of phosphorylation/dephosphorylation on the DNA-binding property of this new 47-kDa protein in particular distinguishes it from other known CREB factors and suggests that the protein might play a unique role in the regulation of cAMP-mediated transcription.
		We have identified in mammalian cells a novel cyclic AMP response element (CRE)-binding protein of molecular mass 47 kDa.
	www.pdg.cnb.uam.es /UniPub/iHOP/gp/7661004.html (215 words)

	Transcriptional Regulation of Fcgr2b Gene by Polymorphic Promoter Region and Its Contribution to Humoral Immune ...
		Southwestern analysis to detect nuclear binding protein, and
		Luciferase activity was normalized by gene dosage estimated by Southern blot hybridization, using the 1.7-kb NcoI-XbaI fragment from the pGL3-Basic vector as a probe.
		Nuclear extract was electrophoresed and hybridized with a radiolabeled probe.
	www.jimmunol.org /cgi/content/full/169/8/4340 (6187 words)

	Medical Dictionary: Western blotting - WrongDiagnosis.com
		Western blotting : identification of proteins or peptides that have been electrophoretically separated by blot transferring from the electrophoresis gel to strips of nitrocellulose paper, followed by labeling with antibody probes.
		Other terms that may be related to Western blotting:
		The description of Western blotting may also be used for the following terms:
	www.wrongdiagnosis.com /medical/western_blotting.htm (208 words)

	Ribin, a Protein Encoded by a Message Complementary to rRNA, Modulates Ribosomal Transcription and Cell Proliferation ...
		The Southwestern technique, probing phage plaques with radiolabeled DNA (3, 40), was used to screen a rat liver cDNA
		Western and Southwestern blotting Immunoblotting was performed following established procedure (38).
		(A) Total cell extracts from hamster BHK cells and mouse N2a cells were probed in Western blotting with antiribin serum (lanes 1 and 2) or preimmune serum (lanes 3 and 4).
	mcb.asm.org /cgi/content/full/21/24/8255 (5670 words)

	SR-Related Protein TAXREB803/SRL300 Is an Important Cellular Factor for the Transactivational Function of Human T-Cell ...
		The gel was blotted onto a polyvinylidene difluoride membrane, denatured with 6 M guanidine hydrochloride, then renatured with the 100% dilution method, and incubated with
		The probe DNA was recovered with streptavidin-coated beads, and the probe-bound proteins were eluted with SDS loading buffer and analyzed by Western blotting with the anti-CREB antibody.
		The levels of CREB and Tax in the lysates used for the binding assay were equal, as shown by immunoblot analysis with a 10% input of lysate.
	jvi.asm.org /cgi/content/full/77/18/10015 (7063 words)

	FEBS Abstract
		The affinity of this protein to AAAF-damaged DNA was ~10-fold higher than to undamaged DNA and its probable size was about 42 kDa, as detected by Southwestern blotting assay.
		copies per cell, and its probable size was about 26 kDa, as detected by the Southwestern blotting assay.
		The affinity of this protein to DNA damaged by AAAF was ~70-fold higher as compared to undamaged DNA.
	www.blackwellpublishing.com /febsabstracts2005/abstract.asp?id=41948 (299 words)

	{alpha}-Melanocortin and Endothelin-1 Activate Antiapoptotic Pathways and Reduce DNA Damage in Human Melanocytes -- ... (Site not responding. Last check: 2007-10-20)
		C, Western blot analysis of total Akt and actin (to control for loading), to verify that the observed change in Akt activity is not due to increased levels of Akt by treatment with UV,
		A, 90 minutes after UV exposure, cell lysates were prepared and Western blot analysis for Mitf was carried out as described in Materials and Methods.
		Southwestern blot analysis was employed to determine induction of CPD immediately (i.e., few minutes) after UV exposure (T0) and their repair 10 and 24 hours after irradiation as described in Materials and Methods.
	cancerres.aacrjournals.org /cgi/content/full/65/10/4292 (4962 words)

	trans-fact-lec30
		SOUTHWESTERN BLOTTING is a method to detect DNA-binding proteins following their
		Southern blot but is incubated with the membrane in the ds form.
		Southwestern blotting provides not only information that a protein capable of interacting with a
	www.hort.wisc.edu /vierstra/Hort550/trans-fact-lec30.htm (1640 words)

	southwest style rugs
		Removing as much moisture from the spill as possible by blotting with a clean, white, absorbent cloth is the key.
		Remember to pretest the solvent on a small area of the rug by applying a few drops, then blotting with a clean, white, absorbent cloth.
		Always, use clean tap water to remove any residual cleaner, and absorb any remaining moisture with dry towels.
	www.strictlysouthwestern.com /rugs.htm (419 words)

	Smad2 Mediates Transforming Growth Factor-{beta} Induction of Endothelial Nitric Oxide Synthase Expression -- Saura et ...
		blotting, nuclear extracts (50 µg of total protein) from
		Total RNA was analyzed by Northern blotting with a radiolabeled eNOS probe.
		B, New protein synthesis is not required for TGF-ß effects on levels of eNOS mRNA.
	circres.ahajournals.org /cgi/content/full/91/9/806 (4699 words)

	The L1 Major Capsid Protein of Human Papillomavirus Type 16 Variants Affects Yield of Virus-Like Particles Produced in ...
		L1 proteins from the six HPV-16 strains were used to investigate DNA binding to L1 protein by Southwestern blotting using
		Detection of DNA binding to HPV-16 L1 proteins in the six strains by Southwestern blotting using a digoxigenin-labelled probe.
		In addition, we have shown by Southwestern blotting that the L1 protein of HPV-16 has the ability to bind to DNA.
	jcm.asm.org /cgi/content/full/36/7/2046 (4142 words)

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