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| | Biophysical Journal: Blind Spectral Decomposition of Single-Cell Fluorescence by Parallel Factor Analysis (Site not responding. Last check: 2007-10-09) |
 | | It is based on the recording of two-dimensional single-cell fluorescence spectra and on the blind spectral decomposition of fluorescence data by method of parallel factor analysis. |
 | | Linear spectral decomposition, or unmixing, is a straight-forward approach to determine the contributions of each fluorophore in the overall fluorescence data, considering the mixed spectrum as the weighted sum of each component, and is practicable when every component spectrum can be measured separately (Dickinson et al., 2001; Zimmermann et al., 2002). |
 | | PARAFAC is one of blind decomposition methods for multiway data, which is originating from psychometrics (Carroll and Chang, 1970) and is recently gaining more interest in the field of chemometrics (Geladi, 1989; Leurgans and Ross, 1992; Wu et al., 2001) for several reasons. |
| www.24hourscholar.com /p/articles/mi_qa3938/is_200403/ai_n9376616 (1356 words) |
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