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Topic: Spermatid

In the News (Fri 13 Nov 09)

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Mickey Rourke

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Hugh Jackman

	Are round spermatid injections of any clincial vallue? ROSNI and ROSI revisited. Sherman J. Silber and Larry Johnson
		In no cases were round spermatids found in the absence of elongated spermatozoa, and maturation arrest was found always to be a failure of progression beyond meiosis (not at maturation from round spermatid to mature elongated spermatid).
		Recently the possibility has been investigated of using 'round spermatids', or 'round cells', derived from testicular tissue (or even from the ejaculate), that are presumably early spermatids, to inject for ICSI for such cases when no elongated spermatozoa are recoverable.
		Their successful pregnancy resulted from injection of a spermatid retrieved from the testicle, rather than the ejaculate, but these were not to round spermatids.
	www.infertile.com /inthenew/sci/rosniros.htm (2941 words)

	Spermatid
		The term spermatid refers to the haploid male gametid that results from division of secondary spermatocytes.
		Spermatids are connected together by cytoplasmic material and have superfluous cytoplasmic material around their nuclei.
		The spermatids begin to grow a tail, develop a thickened mid-piece where the mitochondria become localised and form an acrosome.
	www.mrsci.com /Andrology/Spermatid.php (129 words)

	Spermatid - Wikipedia, the free encyclopedia
		Transverse section of a tubule of the testis of a rat.
		Spermatids are connected together by cytoplasmic material and have superfluous cytoplasmic material around their nuclei.
		The spermatids begin to grow a tail, develop a thickened mid-piece where the mitochondria become localised and form an acrosome.
	en.wikipedia.org /wiki/Spermatid (275 words)

	Spermatid organelles
		In round spermatids, the differentiated nuclear envelope is generally decorated by a thin layer of heterochromatin, often associated with one nucleolus.
		In spermatids, it is isolated, forming a proteic mass surrounded (rat) or not by glycoprotein vesicles.
		In the young round spermatids it is located close to the Golgi apparatus and the centrioles, and it moves backwards with them prior to nuclear elongation.
	www.tours.inra.fr /prc/internet/courtens/spermiogenese/pnsen.php (1231 words)

	Intracytoplasmic Spermatid Injection Can Result in the Delivery of Normal Offspring -- Mansour et al. 24 (5): 757 -- ...
		Sb2) and the elongated spermatids (Sc, Sd1), for ICSI in nonobstructive
		The size of the late spermatid can be compared with the size of a red blood cell and with the diagrammatic presentation of spermatids in the previous figure.
		Spermatid Sb2 (a, b) show the characteristic "ice-cream cone appearance." Spermatid heads (Sd2) (e) are seen within Sertoli cell cytoplasm, showing long tails of variable lengths.
	www.andrologyjournal.org /cgi/content/full/24/5/757 (3668 words)

	Ooplasmic injections of rabbit round spermatid nuclei or intact round spermatids from fresh, cryopreserved and ...
		spermatid nuclei, whereas 43 oocytes were successfully injected
		Within the round spermatid a fl spot (white arrow) representing the acrosomal granule/cap is observable.
		E to stabilize further the plasma membrane of the round spermatid.
	humrep.oxfordjournals.org /cgi/content/full/14/6/1506 (6579 words)

	Cytoplasmic Dynein-Dynactin Complex Is Required for Spermatid Growth but Not Axoneme Assembly in Drosophila -- ...
		The spermatid tails (arrowheads) are tightly bundled and have a membranous bulge (arrow) at their distal ends.
		Arrowhead indicates a single spermatid cell, and arrow indicates enrichment of staining in individual spermatids at the distal ends.
		Growing spermatids remain in syncytium at the distal most end and individual axoneme-mitochondria assembly as shown in this illustration is found to be enveloped in separate axonemal sheath just below the elongation cone region marked by the spectrin rich cytoskeleton.
	www.molbiolcell.org /cgi/content/full/15/5/2470 (8125 words)

	Bioline International Official Site (site up-dated regularly)
		The blepharoplast is not detectable in the polygonal cells until the young spermatids, formed by the last division of the spermatocyte, are beginning to become spherical (Fig.2).
		At this stage, the MLS appears in the spermatid when the blepharoplast is completely differentiated, and a mitochondrion becomes associated closely with the inside the especially at their junction with the microtubular ribbon, which is the outer layer of the MLS (Figs.7, 8).
		Hoffman and Vaughn (1995) used the Centria as the antibody to label the spermatid of the fern Ceratopteris richardii, and found that the blepharoplast was associated with a quantity of antibodies.
	www.bioline.org.br /request?as03019 (4182 words)

	Genetic Analysis of a Y-Chromosome Region That Induces Triplosterile Phenotypes and Is Essential for Spermatid ...
		Images of the spermatid bundles are generally oriented from the apical tip (AP) of the testes (left) to the basal region (right).
		Approximately one-fourth of the ICs in a testis have traversed caudally along the spermatid tails and mature ICs are stained strongly by phalloidin and are visible readily through the sheath of intact testes (data not shown).
		Some of the spermatid tails were probably absent in the cysts, since the bundles were smaller in diameter than normal ones.
	www.genetics.org /cgi/content/full/155/1/179 (5314 words)

	Phosphorylation of Rat Spermatidal Protein TP2 by Sperm-specific Protein Kinase A and Modulation of Its Transport into ...
		B, in-gel kinase assay was carried as mentioned under "Experimental Procedures" using round spermatid cytosol (30–40 µg) as the source of kinase and recombinant, wild-type, unphosphorylated TP2 (0.02% in the resolving gel) as the substrate.
		The effect of phosphorylation of TP2 near its NLS was studied by nuclear import assay using recombinant wild-type and mutant TP2 and the cytosol prepared from elutriated round spermatids.
		in elongating spermatid nucleus to dephosphorylate TP2 and to
	www.jbc.org /cgi/content/full/278/52/52673 (5377 words)

	Acroplaxome, an F-Actin-Keratin-containing Plate, Anchors the Acrosome to the Nucleus during Shaping of the Spermatid ...
		Elongation of the spermatid nucleus occurs as the marginal regions of the acrosome and acroplaxome (dashed boxes) continue their descent.
		in a shallow circular indentation of the spermatid nucleus.
		Rivkin, E., Cullinan, E.B., Tres, L.L., and Kierszenbaum, A.L. A protein associated with the manchette during rat spermiogenesis is encoded by a gene of the TBP-1-like subfamily with highly conserved ATPase and protease domains.
	www.molbiolcell.org /cgi/content/full/14/11/4628 (6321 words)

	Rat Testis Motor Proteins Associated with Spermatid Translocation (Dynein) and Spermatid Flagella (Kinesin-II) -- ...
		Sertoli cell cytoplasm surrounding elongate spermatid heads was highly immunoreactive with the antibody to cytoplasmic dynein (large white arrowheads in C and D).
		Shown here is a spermatid that was mechanically dissociated from a perfusion-fixed testis and labeled with an antibody to the intermediate chain of cytoplasmic dynein (green) and with a fluorescent phallotoxin (red) that binds to filamentous actin.
		At this stage of spermatogenesis, immunoreactivity with the dynein antibody occurred mainly within the spermatid itself and primarily was associated with the manchette.
	www.biolreprod.org /cgi/content/full/60/4/1047 (5923 words)

	Spermatocyte/Spermatid-specific Thioredoxin-3, a Novel Golgi Apparatus-associated Thioredoxin, Is a Specific Marker of ...
		to the Golgi apparatus of mammalian spermatocytes and spermatids.
		migrated to the distal cytoplasmic lobe of the elongating spermatid,
		In both pachytene and round spermatids, the immunoreactivity seems peripheral to the nucleus with a predominately polar orientation, suggestive of the Golgi apparatus.
	www.jbc.org /cgi/content/full/279/33/34971 (6887 words)

	Strength Measurement of the Sertoli-Spermatid Junctional Complex -- Wolski et al. 26 (3): 354 -- Journal of Andrology
		It is theorized that spermatids associated with ESs adhere to
		Hormonal dependency of neural cadherin in the binding of round spermatids to Sertoli cells in vitro.
		A complex containing {alpha}6{beta}1-integrin and phosphorylated focal adhesion kinase between Sertoli cells and elongated spermatids during spermatid release from the seminiferous epithelium.
	www.andrologyjournal.org /cgi/content/full/26/3/354 (3127 words)

	Spermiation Failure Is a Major Contributor to Early Spermatogenic Suppression Caused by Hormone Withdrawal in Adult ...
		of the spermatids in the testis failed to be released.
		Pachytene spermatocyte, round spermatid, and elongated spermatid populations after 1 week of withdrawal of FSH alone, T alone, or FSH plus T compared with those in control animals.
		B, The percentage of elongated spermatids failing to spermiate (the number of retained spermatids in stage IX in millions per hour divided by the number of step 17 and 18 spermatids in millions per hour, multiplied by 100).
	endo.endojournals.org /cgi/content/full/141/8/2779 (5981 words)

	Characterisation of two novel genes required for spermatogenesis in Drosophila.
		Since transcription is essentially switched off on entry into the meiotic divisions it is critical that meiosis is delayed until transcripts needed for differentiation events have accumulated to sufficient levels in primary spermatocytes.
		The "meiotic arrest" genes are essential in males for both the G2-Meiosis I transition and for the onset of spermatid differentiation.
		I have found that the meiotic arrest genes are required for transcription in primary spermatocytes of a suite of spermatid differentiation genes.
	users.ox.ac.uk /~zool0195 (866 words)

	Transcriptional regulation by Modulo integrates meiosis and spermatid differentiation in male germ line -- Mikhaylova ...
		Transcriptional regulation by Modulo integrates meiosis and spermatid differentiation in male germ line -- Mikhaylova et al.
		Transcriptional regulation by Modulo integrates meiosis and spermatid differentiation in male germ line
		meiosis and spermatid differentiation in the male germ line.
	www.pnas.org /cgi/content/abstract/103/32/11975 (310 words)

	Linkage of human spermatid-specific basic nuclear protein genes. Definition and evolution of the P1-->P2-->TP2 ...
		Accordingly we propose that this gene cluster may have arisen from the P1 gene through a series of gene duplication events.
		Protamines and transition proteins are highly basic sperm-specific nuclear proteins that serve to compact the DNA within the condensing spermatid nucleus.
		Sequence and hybridization analysis of this cosmid showed that the TP2 gene resided 3' of the P2 gene at a distance of approximately 7 kilobases (kb).
	www.ihop-net.org /UniPub/iHOP/gp/7648913.html (204 words)

	Spermatid differentiation requires the assembly of a cell polarity complex downstream of junctional adhesion ... (Site not responding. Last check: 2007-11-05)
		Spermatid differentiation requires the assembly of a cell polarity complex downstream of junctional adhesion molecule-C: Nature
		Spermatid differentiation requires the assembly of a cell polarity complex downstream of junctional adhesion molecule-C
		We found that Jam-C is essential for the polarization of round spermatids, a function that we attribute to its role in the assembly of a cell polarity complex.
	www.nature.com /doifinder/10.1038/nature02877 (290 words)

	Infertility and Testicular Defects in Hormone-Sensitive Lipase-Deficient Mice -- Chung et al. 142 (10): 4272 -- ...
		In b some of the late spermatids (circled) are disoriented compared with the organized clusters in a.
		Step 10 elongating spermatids (short arrows) are found simultaneously with step 1 round spermatids (long arrow) and step 11–12 late spermatids (arrowheads).
		Some of the step 9 elongating spermatids present are abnormally oriented within the epithelium (circled), and there is evidence of a very large vacuolated cytoplasmic body (curved arrow) within the epithelium as well as a darkly stained cellular body (open arrow).
	endo.endojournals.org /cgi/content/full/142/10/4272 (5042 words)

	The microtubule plus-end-tracking protein CLIP-170 associates with the spermatid manchette and is essential for ...
		In the wild-type spermatid, the nucleus is surrounded by highly regularly organized MT bundles, which form a well-developed manchette.
		Live images of a portion of a testis tubule were taken, and after a number of frames, the rectangular regions indicated with the number 1 were bleached and time-lapse analysis was resumed.
		Wolosewick, J.J. and Bryan, J.H. Ultrastructural characterization of the manchette microtubules in the seminiferous epithelium of the mouse.
	www.genesdev.org /cgi/content/full/19/20/2501 (7304 words)

	Loss of TSLC1 Causes Male Infertility Due to a Defect at the Spermatid Stage of Spermatogenesis -- van der Weyden et ...
		in spermatids from wild-type or Tscl1 null mice.
		of spermatids and severe spermatozoan malformation (6, 32, 34).
		Spermatid differentiation requires the assembly of a cell polarity complex downstream of junctional adhesion molecule-C. Nature 431:320-324.
	mcb.asm.org /cgi/content/full/26/9/3595 (8003 words)

	Cannonball
		The delayed timing and small number suggest that the spermatids were most likely derived from spermatocytes that had not yet reached the arrest point at the time of the temperature shift.
		Later spermatid stages with elongated flagella were never observed in mutant testes even up to 168 hours (7 days) after the temperature shift.
		Such a cross-regulatory mechanism between the pathways leading to spermatid differentiation and meiosis could serve in wild type to ensure that spermatocytes do not enter meiotic division until the proposed transcription program for post-meiotic spermatid differentiation genes has been successfully initiated.
	www.sdbonline.org /fly/sturtevant/cannonball4.htm (8337 words)

	NGC - NGC Summary
		Round spermatid nucleus injection (ROSNI) is a method of assisted fertilization in which precursors of mature spermatozoa are injected into oocytes.
		ROSNI should not be performed when more mature sperm forms (elongating spermatids or spermatozoa) can be identified and used for intracytoplasmic sperm injection (ICSI).
		Patients who may be candidates for ROSNI should receive careful and thorough pretreatment counseling to ensure they are clearly informed of the limitations and potential risks of the procedure.
	www.guideline.gov /summary/summary.aspx?doc_id=4846&nbr=3487&ss=6&xl=999 (410 words)

	Selective and Continuous Elimination of Mitochondria Microinjected Into Mouse Eggs From Spermatids, but Not From Liver ...
		sperm mitochondria, we microinjected spermatid or liver mitochondria
		is that expression of the machinery that excludes spermatid
		spretus mtDNA in sperm (•) or spermatid (
	www.genetics.org /cgi/content/full/156/3/1277 (4539 words)

	Fertilization, embryonic development, and offspring from mouse eggs injected with round spermatids combined with Ca2+ ...
		Round spermatids, precursor male gametes, are known to possess
		spermatid and sperm factor were recorded in the similar way.
		Sasagawa, I. and Yanagimachi, R. (1996) Comparison of methods for activating mouse oocytes for spermatid nucleus transfer.
	molehr.oxfordjournals.org /cgi/content/full/5/2/132 (4490 words)

	Spermatogenesis in Animals as Revealed by Electron Microscopy: VII. Spermatid Differentiation in the Crab, Eriocheir ...
		Spermatid Differentiation in the Crab, Eriocheir japonicus -- Yasuzumi 7 (1): 73 -- The Journal of Cell Biology
		from the vasa deferentia and spermatids from the testes were
		spermatid, the nucleus shows a concave disc-like contour in
	www.jcb.org /cgi/content/abstract/7/1/73 (344 words)

	CiteULike: uz_labu_laimi's spermatid (Site not responding. Last check: 2007-11-05)
		Recent papers added to uz_labu_laimi's library classified by the tag spermatid.
		Physiologically inactive nucleoli contain nucleoplasmic ribonucleoproteins: immunoelectron microscopy of mouse spermatids and early embryos.
		Failure to assemble the peri-nuclear structures in GOPC deficient spermatids as found in round-headed spermatozoa.
	www.citeulike.org /user/uz_labu_laimi/tag/spermatid (285 words)

	Human Spermatogenesis in Vitro: Respective Effects of Follicle-Stimulating Hormone and Testosterone on Meiosis, ...
		A, Sa spermatid with a still uncondensed nucleus (n) surrounded by a continuous rim of cytoplasm.
		C, Sb2 spermatid with a slightly protruding, partly condensed nucleus (n) and an elongated cell body with a short flagellum (arrow).
		E, Sd1 spermatid with a highly condensed, protruding nucleus (n) and an elongated cell body with a long flagellum (arrows).
	jcem.endojournals.org /cgi/content/full/83/12/4467 (4301 words)

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