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Topic: Stokes radius


In the News (Wed 15 Feb 12)

  
  How to calculate Stokes' radius   (Site not responding. Last check: 2007-09-15)
The Stokes' radius of a protein may be calculated by running it over a gel filtration column.
For the Porath solution of Stokes' radius, the plot Kd^(1/3) versus Stokes' radius is made.
For the Laurent and Killander solution of Stokes' radius, the plot of (-logKav)^(1/2) versus Stokes' radius is made.
itsa.ucsf.edu /~hdeacon/Stokesradius.html   (565 words)

  
 Biophysical Journal: Hydrodynamic properties of human erythrocyte band 3 solubilized in reduced triton X-100
The elution profile of band 3 purified from membranes that had been stripped of its cytoskeleton before solubilization was a broad single peak describing a heterogeneous population of oligomers with a mean Stokes radius of 100 Angstrom.
Whether the conversion of dimer to the form described by a Stokes radius of 100 Angstrom was initiated by removal of cytoskeletal components, alkali-induced changes in band 3 conformation, or alkali-induced loss of copurifying ligands remains unclear.
The ability of the different forms of the protein to bind stilbene disulfonates revealed that the dimer had the highest inhibitor binding affinity, and the form characterized by a mean Stokes radius of 114 Angstrom to have the lowest.
www.findarticles.com /p/articles/mi_qa3938/is_199904/ai_n8849528   (1209 words)

  
 Sharni & Papo
Two limits were defined for Stokes integration with respect to any computation-point: the inner-limit related to the specific grid cell within which the computation point is located, and the outer-limit, beyond which integration is terminated.
Stokes integration between the computation-point and the inner-limit was replaced by evaluation of the effect of a spherical cap.
Thus the total result of Stokes integration at any point within the project area was the sum of three integrals representing the spherical cap, the denser grid and the basic grid.
www.fig.net /pub/proceedings/prague-final-papers/sharni-papo.htm   (3688 words)

  
 Stokes's Law   (Site not responding. Last check: 2007-09-15)
It is based primarily on the radius of the sphere and the viscosity of the fluid.
This is based on a ratio between the sphere radius and the inner radius of the cylinder.
The end effect correction modifies Stokes law to account for the fact that the sphere does not fall indefinitely and is based on the ratio of sphere radius to the total height of the liquid.
www.cord.edu /faculty/ulnessd/legacy/fall1998/sonja/stokes.htm   (392 words)

  
 Characterization of Folded, Intermediate, and Unfolded States of Recombinant Human Interstitial Collagenase -- Zhang ...   (Site not responding. Last check: 2007-09-15)
The unfolded and refolded states of the proteinase were characterized in terms of catalytic constants, Stokes' radius, fluorescence, and CD spectroscopic properties.
Stokes' radii were estimated from the calibration curve as described by Fish et al.
Typically, such intermediates exhibit Stokes' radii that are larger than that of the fully folded protein but smaller than the denatured state.
www.jbc.org /cgi/content/full/271/14/8015   (5762 words)

  
 Molecular characterization of starch with HPSEC-MALLS-RI system   (Site not responding. Last check: 2007-09-15)
Not only the absolute value of molecular weight, but the radius of gyration, oligomeric conformations, and solvent interactions can be measured.
Basic theory behind the analysis is Rayleigh-Debye-Gans model where the scattering intensity of a polymer in a dilute solution depends on its molecular weight and concentration in the solution.
This technique is non- invasive, fast and not dependant upon the Stokes radius of the macromolecules nor calibration curve.
www.aaccnet.org /meetings/99mtg/abstracts/acaba50.htm   (273 words)

  
 Stokes Theorem   (Site not responding. Last check: 2007-09-15)
For a given vector field and surface you are asked to evaluate left- and right-hand parts of Stokes theorem.
The surface itself has been described before, it is a ``half-cylinder'' of radius three.
All that remains is the evaluation of the LHS of Stokes' theorem, i.e.,
www-users.york.ac.uk /~hm126/tutorial_3/node3.html   (827 words)

  
 Analytical Techniques in Aquaculture Research   (Site not responding. Last check: 2007-09-15)
In practice, separation depends on the Stokes radius of a protein, rather than directly on its molecular weight.
The Stokes radius is the average radius that a protein has in solution, and depends on its three dimensional molecular structure.
One problem with this method is that the molecular weight is not directly related to the Stokes radius for different shaped proteins.
allserv.rug.ac.be /aquaculture/ATA/analysis/prot_sep.htm   (2414 words)

  
 Laser Light Scattering
This motion data is conventionally processed to derive a size distribution for the sample, where the size is given by the "Stokes radius" or "hydrodynamic radius" of the protein particle.
Traditionally, rather than presenting the data in terms of diffusion coefficients, the data are processed to give the "size" of the particles (radius or diameter).
It should be noted, however, that the interpretation of data from high concentration samples (and especially the values of the apparent hydrodynamic radius) can be quite difficult due to solution non-ideality ("molecular crowding") effects.
www.ap-lab.com /light_scattering.htm   (1685 words)

  
 Dropping the Ball   (Site not responding. Last check: 2007-09-15)
Note that this drag force is directly proportional to the radius.
Obviously, it depends on the size of the ball:  let’s say the radius is a, and that has dimension L. It must depend on the speed v, which has dimension LT Finally, it depends on the coefficient of viscosity h which has dimensions ML The drag force F has dimensions [F] = MLT
It follows that for two balls of the same density r, after canceling a from each side, the ratio of their terminal velocities is the square of the ratio of their radii, a ball with radius 2a will fall four times faster than a ball with radius a.
landau1.phys.virginia.edu /classes/152.mf1i.spring02/Stokes_Law.htm   (778 words)

  
 Sharni & Papo
At any point within the grid boundaries, geoid undulation can be predicted now by adding the interpolated correction grid value to a "GPM98B plus Stokes" crude prediction.
Radius of Stokes's integration of the residual f.a.
With anchor points spaced 5-20 km apart; employing the GPM98B model and finally extending Stokes's integration up to 2 degrees we obtained an accuracy (one-sigma) of 2 cm or better.
www.fig.net /pub/proceedings/prague/sharni-papo-abs.htm   (466 words)

  
 WCBP'99   (Site not responding. Last check: 2007-09-15)
This family of related compounds (a type of copolymer) is appropriately characterized by traditional polymeric analytical parameters which monitor group properties of the overall mixture.
The GPC calibration yields the Stokes radius and polydispersity (or () of the conjugated protein sample.
The sizing separation also uses a UV detector in series and the peak area data from both detectors is mathematically combined to yield the weight composition (weight of polymer/weight of protein) of the HPLC peak.
www.casss.org /meetings/2002WCBP/1999/kunitani.html   (299 words)

  
 Re: [ccp4bb]: Protein Dimensions
If you are simply after a Stokes radius (single value), then a lot of the software written for small angle X-ray scattering (SAXS) is likely to partially helpful.
and Applied Crystallography pubs) will calculate radii of gyration (Rg), which should be similar to a Stokes radius (with the caveat that this is an X-ray radius which will is influenced by such factors as an ordered water layer (that I believe CRYSOL puts around the pdb input explicitly).
However, I believe it should be similar to the Stoke's radius (and I've pushed assemblies much larger than lysozyme through CRYSOL with success).
www.ysbl.york.ac.uk /ccp4bb/2002/msg00203.html   (506 words)

  
 Student Seminar Home
The size and mass of the solute particle are related in such a way that we can naturally approach the Brownian limit (when the solute becomes much larger and more massive than the solvent particles).
We find that as long as the solute radius is interpreted as an effective hydrodynamic radius, the Stokes-Einstein law with slip boundary conditions is satisfied as the Brownian limit is approached (specifically, when the solute is roughly 100 times more massive than the solvent particles).
We also find that in the Brownian limit the amplitude of the long-time tail of the solute's velocity auto-correlation function is in good agreement with theoretical hydrodynamic predictions.
www.chem.wisc.edu /physical/Abstracts/Fall03/Schmidt_Abstract.htm   (153 words)

  
 Hindered Transport in Biological and Biomimetic Materials   (Site not responding. Last check: 2007-09-15)
Hindered convection was studied by measuring the sieving coefficient (Q) of narrow fractions of Ficoll in agarose and agarose-dextran membranes.
As expected, Q decreased with increasing Stokes-Einstein radius of Ficoll or with increasing concentrations of either agarose or dextran.
The dependence of Q on k and Stokes radius was predicted well by a hydrodynamic theory based on flow normal to the axes of equally spaced, parallel fibers.
aiche.confex.com /aiche/2005/preliminaryprogram/abstract_27174.htm   (510 words)

  
 HMG-CoA REDUCTASE FOUNDATION   (Site not responding. Last check: 2007-09-15)
Comparison of this Stokes radius with the radius calculated from the crystal structure indicated that the active form of P.
The Stokes radius, an S20,w of 11.0, and an estimated ?
The Stokes radii of all standard proteins examined correlated with the inverse error function complement of their partition coefficient, Kd (cf.
www.biochem.purdue.edu /~rodwell/abs.htm   (6228 words)

  
 Scholars   (Site not responding. Last check: 2007-09-15)
This is misleading given the tendency for cadherin to dimerize in vivo.
Alternatively, the SEC column was calibrated according to the Stokes radius of the standard proteins.
Thus, calibration of SEC columns according to the Stokes radius yields more reliable determination of the size of proteins.
www.olemiss.edu /programs/McNair/scholars2004/anazia_gregg.html   (167 words)

  
 Analysis of the oligomeric state of Band 3, the anion transport protein of the human erythrocyte membrane, by size ...   (Site not responding. Last check: 2007-09-15)
The oligomeric state of human Band 3 (Mr = 95,000), the erythrocyte membrane anion exchanger, was examined by size exclusion high performance liquid chromatography in solutions containing the nonionic detergent C12E8 (octaethylene glycol n-dodecyl monoether).
Band 3 was heterogeneous with respect to oligomeric composition, the predominant (70%) species being a dimer that bound 0.57 mg of C12E8/mg of protein (Stokes radius = 78 A, s20,w = 6.9 S).
Variable amounts of larger oligomers were also present; however, no evidence for equilibration between oligomeric species was observed in detergent solution.
www.arclab.org /medlineupdates/abstract_1874731.html   (329 words)

  
 Western wood rat radius Information   (Site not responding. Last check: 2007-09-15)
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rat.9greatsite.info /rat-bike/western-wood-rat-radius.html   (340 words)

  
 Novel Organization and Properties of Annexin 2-Membrane Complexes -- Lambert et al. 279 (12): 10872 -- Journal of ...   (Site not responding. Last check: 2007-09-15)
Influence of the pH on the Stokes Radius of AnxA2—The
Stokes radius of the protein was 2.2 ± 0.01 nm, a value
Stokes radius of AnxA2 as a function of pH.
www.jbc.org /cgi/content/full/279/12/10872   (7503 words)

  
 [No title]   (Site not responding. Last check: 2007-09-15)
Molecular weight of the protein can be determined from SDS gel electrophoresis or sedimentation equilibrium or light scattering or sedimentation coefficient and diffusion or deduced from the Stokes' radius measured by gel filtration.
The use of gel filtration to measure Stokes' radius has the major advantage that it does not require pure protein.
The Stokes' radius is related to molecular weight for many enzymes that are approximately spherical in shape, so the gel filtration column can be calibrated in apparent molecular weight.
horizon.unc.edu /projects/monograph/CD/Professional_Schools/MoBy/04hrm.doc   (2854 words)

  
 Study guide for final exam - key   (Site not responding. Last check: 2007-09-15)
Stoke's radius R = f / 6ph ; with
the Stoke's shift refers to the loss of energy during the excited state of the electron (no radiation) where internal conversion brings the excited electron to the lowest (first) level singlet
In general, vesicles with a radius of >40nm support a equilibrium pressure sufficient for membrane formation.
www-biology.ucsd.edu /classes/bibc110.SU99/guide2key.htm   (1289 words)

  
 Sieve Tube Unloading and Post-Phloem Transport of Fluorescent Tracers and Proteins Injected into Sieve Tubes via ...
Stokes radius of about 11 nm, was unloaded from the SE CC complex.
Assumed relationships between molecular mass and Stokes radii are interpolated from the relationships given by Jorgensen and
Terry BR, Robards AW (1987) Hydrodynamic radius alone governs the mobility of molecules through plasmodesmata.
www.plantphysiol.org /cgi/content/full/123/1/125   (6270 words)

  
 Estimating Molecular Weight   (Site not responding. Last check: 2007-09-15)
This force equation can be expanded in terms of s- value, Stokes' radius, and mass.
Therefore you can write the frictional force on the particle in terms of the eminently measurable Stokes' radius.
Now we have the forces in terms of the sedimentation coefficient, Stokes' radius, and native molecular weight.
itsa.ucsf.edu /~hdeacon/calculate.html   (536 words)

  
 Energy Citations Database (ECD) - Energy and Energy-Related Bibliographic Citations
Energy Citations Database (ECD) Document #5990711 - Stokes radius determination of radioiodinated polypeptide hormones by gel filtration
Availability information may be found in the Availability, Publisher, Research Organization, Resource Relation and/or Author (affiliation information) fields and/or via the "Full-text Availability" link.
Stokes radius determination of radioiodinated polypeptide hormones by gel filtration
www.osti.gov /energycitations/product.biblio.jsp?osti_id=5990711   (88 words)

  
 A monomeric myosin VI with a large working stroke   (Site not responding. Last check: 2007-09-15)
The elution positions for myosin II HMM (350 kDa) (a dimer consisting of two motor domains, four IQ domains with four light chains and a short tail) and myosin II S1(A2) (106 kDa) (a single motor domain and one IQ domain with one A2 light chain bound) are shown.
The elution position of myosin VI is shown, giving it a Stokes radius of 6 nm.
The native molecular weight of myosin VI was calculated using its Stokes radius measured by gel filtration and its sedimentation coefficient determined by sucrose density gradient centrifugation using the following equation as described in Post et al (2002):
www.nature.com /cgi-taf/DynaPage.taf?file=/emboj/journal/v23/n8/full/7600180a.html   (7589 words)

  
 Solution Studies and Structural Model of the Extracellular Domain of the Human Amyloid Precursor Protein -- Gralle et ...
The radius of gyration and the maximum molecular length obtained
The radius of gyration estimated using the Guinier
radius of gyration to the maximum dimension indicates an elongated
www.biophysj.org /cgi/content/full/83/6/3513   (5447 words)

  
 Neither phosphorylation nor the amino-terminal part of rabies virus phosphoprotein is required for its oligomerization ...
The Stokes’ radius of purified P-his was determined by
(a) Determination of the Stokes’ radii of P-his and PA3-his proteins by size-exclusion chromatography.
the expected Stokes’ radius of a globular protein of 29 kDa.
vir.sgmjournals.org /cgi/content/full/81/7/1757   (2944 words)

  
 The Unstable F-box Protein p58-Ctf13 Forms the Structural Core of the CBF3 Kinetochore Complex -- Russell et al. 145 ...
(a) Representation of CBF3 subunits as ellipsoids (with circular cross-sections) whose longest axis is equal to twice the Stokes radius and whose shorter axis is calculated using the experimentally determined mass and a typical value for protein density.
Interactions that have been detected among CBF3 subunits are shown with heavy arrows.
The calculated mass of this complex (450 kD) and the experimentally determined sedimentation coefficient of 15 S yield a Stokes radius of 70 Å.
www.jcb.org /cgi/content/full/145/5/933/F10   (368 words)

  
 Thermoacoustic (Thermal Acoustic) Oscillations
The radius to Stokes boundary layer ratio = r (C
Where r is the tube radius, C the speed of sound and l
The density and the viscosity of the cold gas are represented by
www.yutopian.com /Yuan/TAO.html   (571 words)

  
 A Role for Groucho Tetramerization in Transcriptional Repression -- Chen et al. 18 (12): 7259 -- Molecular and Cellular ...
radius (59.5 Å), derived from gel filtration, and its sedimentation
Fractions with peak levels of native protein standards that were run in a parallel gel filtration experiment are indicated above the gel: thyroglobulin, 669 kDa (Stokes radius, 88 Å); ferritin, 440 kDa (62 Å); catalase, 232 kDa (52.2 Å); aldolase, 158 kDa (48 Å); and bovine serum albumin, 67 kDa (35 Å).
radius and the sedimentation coefficient of Gro, it is possible
mcb.asm.org /cgi/content/full/18/12/7259   (7014 words)

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