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| | BSCI 424 Streaking Technique (Site not responding. Last check: 2007-10-27) |
 | | The most important technique for this purpose is "streaking out" on the surface of a solid nutrient medium, the principle being that a single organism, physically separated from others on the surface of the medium, will multiply and give rise to a localized colony of descendants. |
 | | Sterilize the loop and cool again, then with the edge of the loop, lightly make another set of nearly parallel streaks about 1/8 inch apart, in one direction only, from the inoculated area to one side of the uninoculated area, so that about 1/2 the plate is now covered. |
 | | Streaking from this area is then continued with a wire loop, as above. |
| www.life.umd.edu /classroom/bsci424/LabMaterialsMethods/StreakingTechnique.htm (392 words) |
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