
	Where results make sense

Topic: Subtilisin

In the News (Tue 2 Dec 08)

Damian Green

Sumner Redstone

Crimson Tide

Tommy Tuberville

Bill Clinton

Wayne Rooney

Vilasrao Deshmukh

Wake Forest

Grand Slam

Oklahoma State

	sbt.html (Site not responding. Last check: 2007-11-06)
		Subtilisin is one of a major protein which have a lot of relatives in nature.
		Subtilisin is consist of 8 alpha-helix(blue coil) and 9 beta-strand structure(pale green arrow).
		Subtilisin E is shown by light green and shown at same angle as above.
	homepage.mac.com /yabyab/sbt (218 words)

	Title of Invention: Multiply mutated subtilisins
		Subtilisin DY comprises 274 amino acids and differs from subtilisin Carlsberg in 32 amino acid positions and from subtilisin BPN' by 82 amino acid replacements and one deletion (subtilisin DY lacks an amino acid residue corresponding to residue 56 of subtilisin BPN').
		Subtilisins that may be modified in accordance with the present invention include, but are not limited to, naturally occurring subtilisins represented by the amino acid sequence of subtilisin Carlsberg, subtilisin BPN' the aprA gene product of Bacillus subtilis, subtilisin DY and the subtilisin of Bacillus mesentericus, and the subtilisin of B. subtilis var.
		Heat denaturation is an irreversible reaction for subtilisin: in the absence of protease inhibitor the intact enzyme digests the unfolded form, in the presence of inhibitor the protein precipitates as it unfolds.
	www.nal.usda.gov /bic/Biotech_Patents/1995patents/05397705.html (12227 words)

	Literature for peptidase S08.034 (Site not responding. Last check: 2007-11-06)
		Bott,R., Ultsch,M., Kossiakoff,A., Graycar,T., Katz,B. and Power,S. The three-dimensional structure of Bacillus amyloliquefaciens subtilisin at 1.8Å and an analysis of the structural consequences of peroxide inactivation.
		Nedkov,P., Oberthur,W. and Braunitzer,G. Determination of the complete amino-acid sequence of subtilisin DY and its comparison with the primary structures of the subtilisins BPN', Carlsberg and amylosacchariticus.
		Morihara,K. and Oka,T. Subtilisin BPN': inactivation by chloromethyl ketone derivates of peptide substrates.
	merops.sanger.ac.uk /lit/s08p034_lit.htm (610 words)

	World Intellectual Property Organization (Site not responding. Last check: 2007-11-06)
		In the subtilisin proteases, the relative order of these amino acids reading from the carboxy terminus is aspartate-histidine-serine, while in the chymotrypsin proteases, the relative order of these amino acids reading from the carboxy terminus is histidine-asparate-serine.
		Although subtilisins are typically obtained from bacterial, fungal or yeast sources,"subtilisin"as used herein, refers to a serine protease having the catalytic triad of the subtilisin proteases defined above.
		, a bacterial subtilisin and a fungal subtilisin).
	www.wipo.int /ipdl/IPDL-CIMAGES/view/pct/getbykey5?KEY=03/72746.030904&ELEMENT_SET=DECL (9302 words)

	Supercritical Fluids as Substitutes for Dry Cleaning Solvents: Evaluation of Enzyme Activity for Stain Removal (Site not responding. Last check: 2007-11-06)
		Subtilisin Carlsberg (alkaline protease A) was used as digesting enzyme with supercritical carbon dioxide as an aid in removing a wide variety of clothing contaminants such as food, grass, blood, paint, and ink.
		Since SC CO2 does not solubilize enzymes, separation and recovery of the enzyme after the reaction is easier.
		The Subtilisin Carlsberg exhibits activity in SC CO2 at a wide pressure range from 1000 to 2500 psi.
	www.pprc.org /pprc/rpd/statefnd/turi/supercri.html (358 words)

	[No title]
		Subtilisin NAT was purified to homogeneity (molecular mass, 27.7 kDa) from a saline extract of B. subtilis (natto).
		Subtilisin NAT dose dependently (0.06-1 nm) enhanced tissue-type plasminogen activator-induced fibrin clot lysis both in the absence of rpPAI-1 (48 +/- 1.4% at 1 nm) and especially in the presence of rpPAI-1 (78 +/- 2.0% at 1 nm).
		The stronger enhancement by subtilisin NAT of rpPAI-1-enriched fibrin clot lysis seems to involve the cleavage and inactivation of active rpPAI-1.
	www.prohealth.com /library/print.cfm?ID=2347 (319 words)

	Subtilisin Increases Macromolecular Efflux from the Oral Mucosa -- Rubinstein 7 (5): 794 -- Clinical and Diagnostic ...
		Subtilisin Increases Macromolecular Efflux from the Oral Mucosa
		Effects of subtilisin on leaky site formation (fl bars, upper panel) and clearance of FITC-dextran (molecular mass, 70 kDa; fl bars, lower panel) from the in situ hamster cheek pouch in the absence or presence of CP-96,345, a selective NK receptor antagonist (5 mg/kg i.v.).
		Effects of subtilisin (0.1 µM) on leaky site formation (fl bars, upper panel) and clearance of FITC-dextran (molecular mass, 70 kDa; fl bars, lower panel) from the in situ hamster cheek pouch in the absence or presence of leupeptin, a cysteine proteinase inhibitor (10 µg/ml).
	cdli.asm.org /cgi/content/full/7/5/794 (4075 words)

	Title of Invention: Proteolytic enzymes
		Subtilisins are serine proteases which generally act to cleave internal peptide bonds of proteins or peptides.
		The known Bacillus derived subtilisins are not ideal for all applications as detergent enzymes, in particular, application requiring greater storage stability and activity at broader ranges of pH and temperature.
		The three dimensional structure of proteinase K is similar to that of subtilisins (Paehler, A. et al., 1983, EMBO J. 3, 1311-1314) and there is about 35% homology of the amino acid sequence of proteinase K with that of subtilisins.
	www.nal.usda.gov /bic/Biotech_Patents/1994patents/05278062.html (8544 words)

	Functional and nonfunctional mutations distinguished by random recombination of homologous genes -- Zhao and Arnold 94 ...
		High fidelity DNA shuffling of wild-type subtilisin E gene and 1E2A is described elsewhere ( 5).
		All of the subtilisin E variants were purified as described ( 6).
		Thermostable subtilisin E variant 1E2A was obtained by in vitro evolution of the wild-type enzyme ( 5, 6).
	www.pnas.org /cgi/content/full/94/15/7997 (2709 words)

	Tautomerism, acid-base equilibria, and H-bonding of the six histidines in subtilisin BPN' by NMR -- Day et al. 12 (4): ...
		proton in wild-type subtilisin BPN' in the absence of N-acetyl-L-tryptophan
		N histidine-labeled subtilisin N155A complexed with AAPbF at pH 6.5 and 30°C. Summation of spectra using primary delays of 12, 17, and 22 msec.
		Cassidy, C.S., Lin, J., and Frey, P.A. The deuterium isotope effect on the NMR signal of the low-barrier hydrogen bond in a transition-state analog complex of chymotrypsin.
	www.proteinscience.org /cgi/content/full/12/4/794 (6978 words)

	Basic fibroblast growth factor induction of neuronal ion channel expression in ascidian ectodermal blastomeres -- ...
		Subtilisin- and contact-induced, and non-treated cleavage-arrested (Control) a4-2 cells were also examined for comparison.
		In the case of subtilisin, the required duration was considerably shorter, probably less than 1 h, during a period between 8 and 14 h after fertilization.
		Then, the tyrosine kinase constitutively activated by subtilisin at the beginning of the competent phase, around 8 h after fertilization, may be effective throughout the induction phase from 8 to 14 h after fertilization.
	jp.physoc.org /cgi/content/full/511/2/347 (6932 words)

	Directed Mutagenesis and Protein Engineering
		The enzyme subtilisin is a protease (a protein-digesting protein) produced by bacteria, and has a broad specificity for proteins that commonly soil clothing.
		To improve the efficiency of laundry detergents, detergent manufacturers added subtilisin (and advertised it on the detergent box as "with biologically active enzymes" or with similar uninformative slogans).
		However, subtilisin is inactivated by bleach, and it was found that this inactivation was due to oxidation of the amino acid methionine at position 22 of the subtilisin molecule.
	photoscience.la.asu.edu /photosyn/courses/BIO_343/lecture/protein.html (725 words)

	The Complete Amino Acid Substitutions at Position 131 That Are Positively Involved in Cold Adaptation of Subtilisin ...
		Moreover, we examined the thermal stability of mutant subtilisins along with the wild-type subtilisin in terms of autolysis.
		Cold adaptation of proteins: purification, characterization, and sequence of the heat-labile subtilisin from the antarctic psychrophile Bacillus TA41.
		Cold adaptation of a mesophilic serine protease, subtilisin, by in vitro random mutagenesis.
	aem.asm.org /cgi/content/full/66/4/1410 (3561 words)

	Substrate-induced activation of a trapped IMC-mediated protein folding intermediate - Nature Structural & Molecular ... (Site not responding. Last check: 2007-11-06)
		The intramolecular chaperone (IMC) of subtilisin was used to trap a partially folded, stable crosslinked intermediate conformer (CLIC) through a disulfide bond between mutated IMC and subtilisin.
		Since trapping of CLIC through a disulfide bond forces the interactions of the IMC domain with subtilisin to remain non-native, the consequences of these interactions on a peptide substrate were examined.
		Proteolysis by subtilisin involves: (i) interactions of the substrate with the substrate binding loop in subtilisin ( Fig.
	www.nature.com /cgi-taf/DynaPage.taf?file=/nsmb/journal/v8/n4/full/nsb0401_321.html (3863 words)

	The Crystal Structure of an Oxidatively Stable Subtilisin-like Alkaline Serine Protease, KP-43, with a C-terminal ...
		Streptomyces subtilisin inhibitor complexed with subtilisin BPN' was partially drawn as a stick model, and its carbon atom is colored in orange.
		The Streptomyces subtilisin inhibitor in KP-43 was modeled according to the binding mode of the subtilisin BPN' and inhibitor complex.
		Subtilisin BPN' is drawn transparently and is superimposed on KP-43.
	www.jbc.org /cgi/content/full/279/45/47344 (3621 words)

	Active Subtilisin-Like Protease from a Hyperthermophilic Archaeon in a Form with a Putative Prosequence -- Kannan et ...
		Subtilisins are synthesized in the cells as a precursor called preprosubtilisin, in which the presequence and prosequence
		The three-dimensional structure of Bacillus amyloliquefaciens subtilisin at 1.8 Å and an analysis of the structural consequences of peroxide inactivation.
		A hyperthermostable protease of the subtilisin family bound to the surface layer of the archaeon Staphylothermus marinus.
	aem.asm.org /cgi/content/full/67/6/2445 (4576 words)

	Modulation of kinesin binding by the C-termini of tubulin (Site not responding. Last check: 2007-11-06)
		In that work, we could show that subtilisin treatment did not affect the position of the neck in dimeric constructs ( Figure 6 ; compare cyan and magenta volumes).
		We also suggest that this effect may be the major reason for the lower processivity reported for kinesin by Wang and Sheetz (2000), as a strongly bound ADP state probably stalls the motor.
		In addition, subtilisin treatment does not seem to affect the flexible nature of the neck-linker in nucleotide-free (see also Skiniotis et al, 2003) and, presumably, ADP conditions.
	www.nature.com /cgi-taf/dynapage.taf?file=/emboj/journal/v23/n5/full/7600118a.html (7130 words)

	Molecular and Biotechnological Aspects of Microbial Proteases -- Rao et al. 62 (3): 597 -- Microbiology and Molecular ...
		subtilisin Carlsberg and subtilisin Novo or bacterial protease
		of protease, a subtilisin or alkaline protease and a metalloprotease
		YaB, a new subtilisin from an alkalophilic Bacillus strain ( 129).
	mmbr.asm.org /cgi/content/full/62/3/597 (9620 words)

	Progressive Rearrangement of Subtilisin Carlsberg into Orderly and Inflexible Conformation with Ca2+ Binding -- Lee and ...
		Subtilisins comprise a group of serine proteases that are secreted in large amounts from a wide variety of Bacillus species.
		Subtilisin enzymes: a note on time-resolved fluorescence and circular dichroism properties.
		The high-resolution X-ray crystal structure of the complex formed between subtilisin Carlsberg and eglin c, an elastase inhibitor from the leech Hirudo medicinalis.
	www.biophysj.org /cgi/content/full/81/5/2972 (3324 words)

	Subtilisin (Site not responding. Last check: 2007-11-06)
		Subtilisins, or carbonyl hydrolases, are alkaline proteases that are secreted by members of the genus Bacillus.
		"Proteases of Enhanced Stability: Characterization of a Thermostable Variant of Subtilisin", Bryan, P.N., Rollence, M.L., Pantoliano, M.W., Wood, J., Finzel, B.C., Gilliland, G.L., Howard, A.J., and Poulos, T.L., Protein: Struc.
		"Directed Evolution of a Subtilisin with Calcium-Independent Stability", Strausberg, S.L., Alexander, P.A., Gallagher, D.T., Gilliland, G.L., Barnett, B.L., and Bryan, P.N., Bio/Technology 13, 669-673 (1995).
	www.carb.nist.gov /gilliland_group/molecules/subtilisin.html (495 words)

	Direct proton magnetic resonance determination of the pKa of the active center histidine in thiolsubtilisin -- ...
		Superposition of the active center residues of subtilisin (cyan) and thiolsubtilisin (colored by atom type) where the sulfur atom is shown in yellow.
		Alden, R.A., Wright, C.S., Westall, F.C., and Kraut, J. Subtilisin BPN`: Tertiary structure and inhibitor binding.
		Petsko, G.A. and Neidhart, D.J. The refined structure of subtilisin Carlsberg at 2.5 Å resolution.
	www.proteinscience.org /cgi/content/full/11/4/965 (5267 words)

	Subtilisin Prodomain (Site not responding. Last check: 2007-11-06)
		The biosynthesis of subtilisin is dependent on a 77 amino acid prosequence, which is eventually cleaved form the N-terminus.
		The structure of the complex of the prodomain and subtilisin suggests how the prodomain may catalyze subtilisin folding.
		In the complex with subtilisin, the prodomain packs against the two parallel surface helices of subtilisin and supplies caps to the N-termini of the two helices.
	www.carb.nist.gov /gilliland_group/molecules/subt_pro.html (232 words)

	Cation-Binding Sites of Subtilisin Carlsberg Probed with Eu(III) Luminescence -- Lee and Jang 79 (4): 2171 -- ...
		Subtilisins comprise a group of serine endopeptidases that are secreted in large amounts from a wide variety of Bacillus species.
		Kinetic analysis of the mechanism for subtilisin in essentially anhydrous organic solvents.
		Fluorescence spectroscopic study of subtilisins as relevant to their catalytic activity in aqueous-organic media.
	www.biophysj.org /cgi/content/full/79/4/2171 (3574 words)

	A subtilisin-like serine protease involved in the regulation of stomatal density and distribution in Arabidopsis ...
		A common feature of subtilisins is their catalytic triad, consisting of three domains: the D region, the H region, and the
		Barr, P.J. Mammalian subtilisins: The long-sought dibasic processing endoproteases.
		Cucumisin, a serine protease from melon fruits, shares structural homology with subtilisin and is generated from a large precursor.
	www.genesdev.org /cgi/content/full/14/9/1119 (7650 words)

	Preparations and properties in organic s
		Here, we report on a methodology suitable to prepare subtilisin Carlsberg and lipase from Pseudomonas cepacia (lipase PS) in a form which can be dissolved or highly dispersed in organic solvents, thanks to the formation of stable non-covalent protein-PEG complexes (subtilisin + PEG and lipase PS + PEG).
		Transesterification, hydrolytic and total (transesterification plus hydrolytic) activity of subtilisin and subtilisin + PEG in 1,4-dioxane, and of lipase PS and lipase PS + PEG in 1,4-dioxane and carbon tetrachloride ( 7,8).
		a The reaction of vinyl butyrate with 1-octanol (or 1-hexanol in the case of subtilisin) was employed as a model and both transesterification (formation of 1-octyl butyrate or 1-hexyl butyrate) and hydrolytic (formation of butyric acid from vinyl butyrate) activities were measured.
	homepages.strath.ac.uk /~cdbs05/carrea.htm (1314 words)

	Subtilisin (Site not responding. Last check: 2007-11-06)
		They exhibit high specific activity on proteinaceous substrates, function optimally at moderate temperatures, and are stable under alkaline conditions.
		Subtilisin gains part of its stability by binding calcium.
		Travis Gallagher is now part of the Bioprocess Engineering Group at NIST.
	www.cstl.nist.gov /div831/carb/gilliland_group/molecules/subtilisin.html (495 words)

	Properties of a subtilisin-like proteinase from a psychrotrophic Vibrio species: Comparison with proteinase K and ... (Site not responding. Last check: 2007-11-06)
		Subtilases belonging to the subtilisin, thermitase and proteinase
		Purification, characterization, and sequence of the heat-labile subtilisin from the antarctic psychrophile Bacillus TA41.
		Narinx, E., Baise, E. and Gerday, C. (1997) Subtilisin from psychrophilic antarctic bacteria: characterization and site-directed mutagenesis of residues possibly involved in the adaptation to cold.
	www.ejbiochem.org /cgi/content/full/260/3/752 (5945 words)

	Generation of a broad esterolytic subtilisin using combined molecular evolution and periplasmic expression -- Sroga and ...
		Generation of a broad esterolytic subtilisin using combined molecular evolution and periplasmic expression -- Sroga and Dordick 14 (11): 929 -- Protein Engineering Design and Selection
		Generation of a broad esterolytic subtilisin using combined molecular evolution and periplasmic expression
		subtilisin and this may have important applications in synthesis.
	peds.oupjournals.org /cgi/content/abstract/14/11/929 (196 words)

	Two Paralogous Families of a Two-Gene Subtilisin Operon Are Widely Distributed in Oral Treponemes -- Correia et al. 185 ...
		Two Paralogous Families of a Two-Gene Subtilisin Operon Are Widely Distributed in Oral Treponemes -- Correia et al.
		Two Paralogous Families of a Two-Gene Subtilisin Operon Are Widely Distributed in Oral Treponemes
		the subtilisin family of proteases and are the only subtilisin
	jb.asm.org /cgi/content/abstract/185/23/6860 (339 words)

	Studies of the Bifunctional Alpha-Amylase/Subtilisin Inhibitor from Barley
		The inhibition of bacterial subtilisin by the same protein may be associated with a defense role for this protein.
		Bacterial expression of the bifunctional alpha-amylase subtilisin inhibitor from barley.
		The amino acid sequence of a 20 k Da bifunctional subtilisin alpha-amylase inhibitor from brain of rice ( Oryza sativa L) seeds.
	www.regional.org.au /au/abts/1999/henry.htm (610 words)

Try your search on: Qwika (all wikis)