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| | Fission yeast retrotransposon Tf1 integration is targeted to 5' ends of open reading frames -- Behrens et al. 28 (23): ... |
 | | DNA from G418-resistant clones was cut with restriction enzymes as indicated, blotted, and hybridised to a Tf1-specific probe shown in the schematic presentation of the nmtTf1-neo fusion construct. |
 | | As controls, DNA from an isogenic strain without Tf1 insertion (wt) and from the same strain carrying the pHL414 plasmid (pHL414) in the presence of thiamine were used. |
 | | Long bars are used to represent two Tf1 insertions which occurred at about the same distance to the closest ORF (clone 8, 141 bp and clone 39, 144 bp; clone 2, 258 bp and clone 29, 261 bp; clone 21, 307 bp and clone 26, 308 bp). |
| nar.oxfordjournals.org /cgi/content/full/28/23/4709 (4350 words) |
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