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  Thermus aquaticus - Wikipedia, the free encyclopedia
Thermus aquaticus is a species of bacterium that can tolerate high temperatures; it is the source of the heat-resistant enzyme Taq DNA Polymerase, one of the most important enzymes in molecular biology because of its use in the polymerase chain reaction.
Studies of this extreme thermophilic bacterium that could be grown in cell culture was initially centered on attempts to understand how protein enzymes (which normally inactivate at high temperature) can function at high temperature in thermophiles.
Those who know Mullis such as Thomas J. White agree that it was Mullis who came up with the idea of using Taq polymerase in order to avoid having to add polymerase to the PCR reaction during the thermocycling process.
en.wikipedia.org /wiki/Thermus_aquaticus   (991 words)

  
 - Taq DNA Polymerase
Since the sequence of Pierce Taq is identical to the sequence of AmpliTaq DNA Polymerase, we expect this value to be the same as that quoted for AmpliTaq DNA Polymerase.
Because the sequence of Pierce Taq is identical to the sequence of AmpliTaq DNA Polymerase, we expect this value to be the same as that quoted for AmpliTaq DNA Polymerase.
According to data generated by DNA sequencing, the sequence of the Pierce Taq gene is identical to the sequence of the AmpliTaq DNA Polymerase gene.
www.piercenet.com /browse.cfm?fldID=041FE7CE-4ADB-4A8F-8E9D-CB32E5409795   (642 words)

  
 [No title]
DNA sequence analysis of Taq and flank- ing lacZ DNA for the candidate from the 12-mer library indicated the presence of one EcoRI linker at its 5' lacZ junction.
DNA sequence analysis of the Taq and flanking lacZ DNA for the 115-bp candidate from the 10-mer library indi- cated the presence of three EcoRI linkers at the 5' lacZ junction, which resulted in the same frame with respect to,6- galactosidase as that of the 12-mer linker candidate.
Indeed, the DNA sequence of the AluI genomic fragment isolated in Xgtl 1 1 corresponds to nucleotides 619-720 in the Taq Pol I gene (Fig.
sunsite.berkeley.edu /PCR/ebind/013/hi-res/013_02.txt   (995 words)

  
 [No title]
Taq DNA polymerase (Taq Pol Dl isolated from Thermus aquaticus has been shown to be highly useful in the polymer- ase chain reaction (PCR) method (1, 2) of amplifying DNA fragments (3).
Therefore, we sought to clone the Taq Pol I gene and express the gene in E. colt.
Upon screening with primary antibody from Taq Pol I-immunized rabbits and plaque purification, we identified seven positive plaques from the 12-mer library, four positive plaques from the 10-mer library, and no positive plaques from the 8-mer library.
sunsite.berkeley.edu /PCR/ebind/013/hi-res/013_01.txt   (655 words)

  
 DFS-Taq DNA Polymerase
Description DFS (DNA Free Sensitive) Taq DNA Polymerase is a thermostable enzyme of approximately 94kDa isolated from eubacterium Thermus aquaticus strain YT-1(1).
Applications DNA-free Taq DNA polymerase is suitable for all regular applications – PCR, primer extension reactions etc. DFS Taq DNA polymerase is free from bacterial DNA and it is especially recommended for the work with bacterial DNA.
In contrast to Bioron enzyme, Taq DNA polymerases from the variety of suppliers contain contaminating DNA, with DNA-contaminated Taq DNA polymerase one should be ready to observe false-positive PCR results in some cases.
www.bioron.net /taq_dna_polymerase.htm   (450 words)

  
 Structure-specific DNA-induced Conformational Changes in Taq Polymerase Revealed by Small Angle Neutron Scattering -- ...
The DNA polymerase I from Thermus aquaticus (Taq polymerase)
in vivo the DNA polymerase I from Thermus aquaticus (Taq polymerase)
Taq polymerase fills the gap, and it is responsible for both synthesizing a new DNA strand and removing the RNA primer to generate a ligatable nick.
www.jbc.org /cgi/content/full/279/37/39146   (5638 words)

  
 Insertion of the T3 DNA polymerase thioredoxin binding domain enhances the processivity and fidelity of Taq DNA ...
with the wild-type Taq DNA polymerase or to the recombinant
Taq DNA pol/TBD and Taq DNA pol/TBD(exo–) are as thermostable
In the absence of the thioredoxin cofactor, the Taq
nar.oxfordjournals.org /cgi/content/full/31/16/4702   (4792 words)

  
 CLP Products   (Site not responding. Last check: 2007-10-12)
Taq DNA Polymerase is a robust, thermal stable DNA polymerase purified from Thermus aquaticus.
Taq has a high 5’ to 3’ activity and no 3’ to 5’ exonuclease activity.
TAQ DNA Polymerase with 10X Ammonium Sulfate Buffer, 5 units/ul, 500 Units
www.clpdirect.com /clpdirect/product_item.aspx?prodid=TAQDNA   (106 words)

  
 Mendoza College of Business - Research Technologies: TAQ   (Site not responding. Last check: 2007-10-12)
TAQ does not include transaction data that is reported outside of the Consolidated Tape hours of operation.
The current available TAQ data is for the period 2000.7 to 2000.10.
The file is written in character format with a fixed record length of 51 bytes (53 including carriage return and line feed).
www.nd.edu /~cbit/academic/cb_data/taq/datataq.htm   (445 words)

  
 Global Conformations, Hydrodynamics, and X-ray Scattering Properties of Taq and Escherichia coli DNA Polymerases in ...
Taq and full-length Pol 1 are in an elongated conformation
The data for Taq and Pol 1 have been displaced on the y axis by multiplying the original intensity values by factors of 0.25 and 10, respectively, for visual clarity.
Protein concentrations are 5.1 mg/ml for Taq, 3.8 mg/ml for Klentaq, and 5.3 mg/ml for Klenow.
www.jbc.org /cgi/content/full/278/28/25341   (4047 words)

  
 Thermostable DNA Polymerases   (Site not responding. Last check: 2007-10-12)
Roughly 10 years later, the polymerase chain reaction was developed and shortly thereafter "Taq" became a household word in molecular biology circles.
Currently, the world market for Taq polymerase is in the hundreds of millions of dollars each year.
In addition to Taq DNA polymerase, several other thermostable DNA polymerases have been isolated and expressed from cloned genes.
arbl.cvmbs.colostate.edu /hbooks/genetics/biotech/enzymes/hotpolys.html   (429 words)

  
 Clontech Laboratories, Inc. - January Clontechniques: BD Taq Full DNA Polymerase   (Site not responding. Last check: 2007-10-12)
Taq Full DNA polymerase is also available with a hot start antibody (Taq Full Hot Start DNA polymerase), providing heightened performance and guaranteeing increased specificity and yield.
Taq Full DNA polymerase is a highly efficient, full-length recombinant version of the Thermus aquaticus (Taq) strain YT1 DNA polymerase (1).
Taq Full polymerase's efficiency and its durability to withstand a high number of PCR cycles means that you can obtain the higher yields you need for your specific PCR application.
www.clontech.com /clontech/archive/JAN05UPD/taq.shtml   (971 words)

  
 DNA Polymerases: Taq DNA Polymerase (recombinant)
Taq DNA Polymerase is a highly thermostable DNA polymerase of a thermophilic bacterium Thermus aquaticus.
Taq DNA Polymerase catalyzes 5'=>3' synthesis of DNA.
Taq buffer with (NH Amplification of 950 bp single copy gene from human genomic DNA using Taq DNA Polymerase (recombinant).
www.fermentas.com /catalog/modifyingenzymes/taqdnapolymeraserecomb.htm   (429 words)

  
 MoBiTec: DNA and Protein Tools   (Site not responding. Last check: 2007-10-12)
Taq DNA polymerase (from Thermus aquaticus expressed in E.
Taq polymerase can be used for amplification of DNA fragments by the polymerase chain reaction (PCR), amplification of single-copy genes from genomic DNA, for cycle-sequencing and for DNA-labeling.
SUPER TAQ is offered in two concentrations: Regular SUPER TAQ comes in 5 units/µl and is suited for general purposes.
www.mobitec.de /mobitec_de/products/dna_prot_tools/pc_supertaq.html   (1016 words)

  
 Taq polymerase
Different concentrations of a Taq polymerase were tested using primer mixture C (Fig.
Five native Taq polymerases, from five different sources, were used to amplify multiplex mixture D in 1.6x PCR buffer using 2Units enzyme/25µl reaction (Fig.
Multiplex PCR of mixture D in 1.6x PCR buffer using Taq polymerases from five sources.
info.med.yale.edu /genetics/ward/tavi/p12.html   (288 words)

  
 Taq FAQ   (Site not responding. Last check: 2007-10-12)
Taq DNA Polymerase was originally isolated from the thermophilic bacterium Thermus aquaticus.
Taq DNA Polymerase is a monomeric enzyme with an apparent molecular weight (by SDS-PAGE) of 94,000Da.
What is the difference between Promega's Taq DNA Polymerase in Storage Buffer A and Storage Buffer B? Taq DNA Polymerase in Storage Buffer A is recommended for use only with Promega's 10X Reaction Buffer.
www.astbury.leeds.ac.uk /jjweb/wwwprg/taq.html   (729 words)

  
 Structure-based design of Taq DNA polymerases with improved properties of dideoxynucleotide incorporation -- Li et al. ...
Structure-based design of Taq DNA polymerases with improved properties of dideoxynucleotide incorporation -- Li et al.
The Taq DNA polymerase is the most commonly used enzyme in DNA sequencing.
Taq DNA polymerase may be involved in sequence-context recognition.
www.pnas.org /cgi/content/full/96/17/9491   (3482 words)

  
 Le Tribunal Administratif du Québec - Your Proceeding with the TAQ - The Hearing   (Site not responding. Last check: 2007-10-12)
Your file is now complete and you are ready to proceed; you will be receiving a summons to the hearing.
It is important to finish the final preparations, especially the summoning of your witnesses.
The Act respecting administrative justice provides that the proceedings at the TAQ are generally heard by a multidisciplinary panel of two members.
www.taq.gouv.qc.ca /english/recours-taq/audience.jsp   (360 words)

  
 Taq-e-Bustan
This Taq shows Shahpur III who succeeded Ardeshir II standing next to his father Shahpur II (AD 310-379), the great Sassanian Emperor.
Close view of the central Taq shows only images of Shahpur III and Shahpur II with no detailed decorations or any investiture scene.
Adjacent to three Taqs stands the ruins of a majestic building; stone made ornaments carved in Sassanian style depict images of Investiture.
www.vohuman.org /SlideShow/Taq-e-Bustan/Taq-e-Bustan-Main.htm   (917 words)

  
 Protocol for PCR using Taq DNA Polymerase
Although even trace amounts of agents used in DNA purification procedures (phenol, EDTA, Proteinase K, etc.) strongly inhibit Taq DNA Polymerase, ethanol precipitation of DNA and repetitive treatments of DNA pellets with 70% ethanol is usually effective in removing traces of contaminants from the DNA sample.
In the presence of such additives, the annealing temperature should be adjusted experimentally, since the melting temperature of the primer-template DNA duplex decreases significantly when these additives are used.
The amount of enzyme in the reaction mix should be increased since DMSO and formamide, at the suggested concentrations, inhibit Taq DNA Polymerase by approx.
www.fermentas.com /techinfo/pcr/dnaamplprotocol.htm   (1509 words)

  
 Different methylation with normal Taq and High fidelityTaq - BioForum
I don't think that the high fidelity taq converts T (actually U) back to C. The problem is unmethylated Cs are converted to U and the modified DNA is actually similar to RNA.
Probably the high fidelity taqs have problem reading the U (they are intended for DNA).
I would suggest you use JumpStart Taq from sigma which doesn't have this problem with giving you very high amplifying efficiency.
www.protocol-online.org /forums/index.php?showtopic=6480   (550 words)

  
 Is there anybody use Taq polymerase of Fermemtas - BioForum
Is there anybody use Taq polymerase of Fermentas(MBI), or other products of this company, its Taq is so cheap that i'm afraid.....
Ive used different kinds of their polymerases (Taq, Pfu) and theyve worked flawlessly...tackling some products ive couldnt amplify with other brands.
I'm used to perform PCR with the Fermentas Taq and I think it is as good as others',which are usually more expensive.
www.protocol-online.org /forums/index.php?act=findpost&pid=10685   (579 words)

  
 We performed a test using DNA Taq Polymerases from six different companies to compare the percent of samples with ...
We performed a test using DNA Taq Polymerases from six different companies to compare the percent of samples with single band
We performed a test using Taq DNA Polymerases from six different companies to compare the percent of samples with single band PCR products.
The cycling parameters (pre-heating step), as well as the amounts of Taq, dNTPs and buffers were according to the company’s general guidelines.
wheat.pw.usda.gov /microarray/Pol_report.htm   (426 words)

  
 [No title]
SureStart Taq can be incorporated into PCR protocols previously optimized with Taq DNA polymerase, with little or no modification of cycling parameters or reaction conditions.
SureStart Taq DNA polymerase consists of a reversibly inactivated version of Stratagene’s Taq2000™ DNA polymerase, a highly purified recombinant Taq DNA polymerase preparation.
SureStart® Taq DNA Polymerase Increases Specificity: A 105 bp fragment of the glucocerebrosidase gene was amplified from human genomic DNA.
www.stratagene.com /products/displayProduct.aspx?pid=101   (542 words)

  
 Affinity maturation of a Taq DNA polymerase specific affibody by helix shuffling -- Gunneriusson et al. 12 (10): 873 -- ...
Affinity maturation of a Taq DNA polymerase specific affibody by helix shuffling -- Gunneriusson et al.
Affinity maturation of a Taq DNA polymerase specific affibody by helix shuffling
Taq DNA polymerase was characterized by a threefold higher apparent
peds.oxfordjournals.org /cgi/content/abstract/12/10/873   (383 words)

  
 Kx Systems Announces FTP Loader for NYSE TAQ Data
By using the new kdb/taq FTP option, Kx customers can FTP the NYSE TAQ data directly into kdb/taq at the end of every trading day, enabling them to perform relational time-series analysis on billions of NYSE TAQ data records and obtain results in seconds.
"Unless NYSE TAQ subscribers are loading the data into an analytical database such as kdb/taq, they aren't taking full advantage of the benefits of their TAQ data," said Arthur Whitney, Kx Systems CTO.
Or they must import the TAQ data into another database, which typically cannot handle the volumes of data at the analysis speeds desired by traders today." Kx introduced kdb/taq in 2001 in order to provide traders with the ability to perform high-speed loading of NYSE TAQ data.
www.prnewswire.com /cgi-bin/stories.pl?ACCT=104&STORY=/www/story/10-29-2002/0001830146&EDATE=   (525 words)

  
 USB - Category - PCR   (Site not responding. Last check: 2007-10-12)
The A-Taq-IT PCR Kit combines USB Taq DNA Polymerase and ExoSAP-IT in one kit to offer a complete solution for amplification and purification of PCR products.
USB Taq is rigorously tested for function and has no detectable contaminating endonuclease or exonuclease activities.
By combining USB Taq DNA Polymerase with ExoSAP-IT, amplification and purification can be accomplished by using just one kit.
www.usbweb.com /category.asp?cat=pcr&id=71175   (190 words)

  
 Invitrogen's AccuPrime™ Taq DNA Polymerase - Biocompare Product Review
The AccuPrime™ Taq DNA Polymerase from Invitrogen is designed to prevent mispriming throughout all cycles of PCR to amplify only the specific DNA targets of interest, without non-specific bands, for accurate performance and specificity in PCR.
For all of these reactions (where multiple bands were seen) we then used the AccuPrime™ Taq DNA Polymerase system and achieved a single PCR band of the correct size in about 90% of the reactions.
In summary, AccuPrime™ Taq DNA Polymerase system is a great product that is virtually guaranteed to give a better PCR result when Taq Polymerase fails.
www.biocompare.com /prorev.asp?profrevid=172   (523 words)

  
 Arrow Taq Polymerase   (Site not responding. Last check: 2007-10-12)
Arrow Taq™ DNA Polymerase (patent pending) is a novel mixture of thermostable DNA polymerases which offers several advantages over PCR with recombinant Taq DNA polymerase alone.
Because Arrow Taq™ is a mixture of Taq DNA Polymerase and Tfu DNA Polymerase which has proofreading capability, it exhibits a lower error rate than Taq.
The presence of Taq means there are few constraints regarding amplification parameters which means less optimization is necessary than when a proofreading enzyme is used alone.
www.qbiogene.com /products/pcr/arrow-taq.shtml   (191 words)

  
 Affinity maturation of a Taq DNA polymerase specific affibody by helix shuffling -- Gunneriusson et al. 12 (10): 873 -- ...
Overlay plot of sensorgrams obtained for twelve affinity matured Taq DNA polymerase specific affibodies when injected at an approximate concentration of 50 nM over a sensor chip surface with immobilized Taq DNA polymerase.
Taq DNA polymerase binding determinants present in the unaltered
Taq DNA polymerase from crude Escherichia coli lysates in which
peds.oxfordjournals.org /cgi/content/full/12/10/873   (3509 words)

  
 Taq DNA Polymerase
When researchers think Taq, many researchers think Promega.
Promega has two options for Taq DNA Polymerase: Taq in Storage Buffer A and Taq in Storage Buffer B. Taq in A is the original Taq offered by Promega and still preferred by many customer worldwide.
Taq in Storage Buffer B is the most popular Taq DNA Polymerase sold by Promega and can be used with our 10X buffer or virtually anyone’s 10X reaction buffer.
www.promega.com /applications/pcr/featuresandbenefits/Taq_DNA_Polymerase.htm   (231 words)

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