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| | Protocol for PCR using Taq DNA Polymerase |
 | | Although even trace amounts of agents used in DNA purification procedures (phenol, EDTA, Proteinase K, etc.) strongly inhibit Taq DNA Polymerase, ethanol precipitation of DNA and repetitive treatments of DNA pellets with 70% ethanol is usually effective in removing traces of contaminants from the DNA sample. |
 | | In the presence of such additives, the annealing temperature should be adjusted experimentally, since the melting temperature of the primer-template DNA duplex decreases significantly when these additives are used. |
 | | The amount of enzyme in the reaction mix should be increased since DMSO and formamide, at the suggested concentrations, inhibit Taq DNA Polymerase by approx. |
| www.fermentas.com /techinfo/pcr/dnaamplprotocol.htm (1509 words) |
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