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Ciliate

Tetrahymena - Wikipedia, the free encyclopedia Tetrahymena are ciliate protozoa, common in aquatic habitats. They are pear-shaped and 30-100 μm in length, belonging among the hymenostomes and with cilia typical of that group. Tetrahymena thrive at temperatures of 18-22 degrees Celsius. en.wikipedia.org /wiki/Tetrahymena   (99 words)

Genetics Tetrahymena (and most Ciliates) are remarkably advanced among unicellular eukaryotes: no cell reproduction in the haplophase; differentiation of somatic and germ line nuclei; binary fission in the face of highly specialized compound cell structures; conjugation in diplophase; and internal fertilization at conjugation. The somatic ciliature in Tetrahymena is distributed throughout the cell and organized in a regular array of rows (or meridians) of ciliary units in the cell cortex [illustrated in ref. 2, p.180]. Hill,DL (1972): The Biochemistry and Physiology of Tetrahymena. lifesci.ucsb.edu /~genome/Tetrahymena/genetics.htm   (7567 words)

Tetrahymena Group Tetrahymena thermophila is a microbial eukaryote and a model system of proven utility. The genes chosen are a subset of those found in Tetrahymena that are involved in processes of significance for human biology and disease that cannot be studied in yeast. For the time being it would seem most cost effective to affiliate with an existing genome database, adopt their format and augment their resources to support this effort. www.nih.gov /science/models/nmm/tetrahymena.html   (448 words)

John E. Hearst   (Site not responding. Last check: 2007-10-22) Tetrahymena is a unicellular, aerobic eukaryote typically found in fresh water. The normal process of digestion in Tetrahymena involves the fusion of ciliate lysosomes with food vacuoles and the introduction of hydrolytic enzymes into the vacuoles. Two days later the Tetrahymena culture was placed under anaerobic stress in the light in a nine mililiter glass tube with a teflon-sealed screw cap to prevent air exchange. www.lbl.gov /sbdiv/ResInts/Projects/Hearst.html   (1982 words)

Team Tetrahymena   (Site not responding. Last check: 2007-10-22) Tetrahymena thermophila is a unicellular, ciliated freshwater protozoan. Tetrahymena can be induced to mate in large synchronous cultures. One particularly striking feature of Tetrahymena mating is that at one particular time during conjugation, different nuclei within a common cytoplasm are simultaneously either (1) undergoing programmed nuclear elimination, (2) undergoing programmed gene rearrangement and chromosome amplification, or (3) remaining developmentally inert. www.stolaf.edu /depts/biology/tetrahymena   (213 words)

Untitled Document   (Site not responding. Last check: 2007-10-22) 2004 The Regulation of RAD51 Expression in the Ciliate Tetrahymena thermophila. and Romero, D.P. A ß Tubulin Mutation that Confers Sensitivity to Paclitaxel Disrupts Macronuclear Division in Tetrahymena. 1991 Cole, E.S. and Stuart, K.R. Biochemical and cytological evidence for an over-abundance of mucocysts in the bcd pattern mutant of Tetrahymena thermophila. www.stolaf.edu /people/colee/publications.htm   (410 words)

Center News - 7/3/03 - Tetrahymena Tetrahymena’s genetic mystique arises from a curious feature of all ciliates, the family of single-celled creatures named for the hair-like structures, known as cilia, which line their surfaces. To their surprise, the gene was deleted from the macronucleus, suggesting that the mic is able to survey its genome for the presence of foreign DNA and to somehow communicate to the mac that the foreign DNA should be removed. Yao said that Tetrahymena’s ability to delete sequences may represent an ancient process that first evolved to protect the cell against transposons, mysterious bits of DNA of unknown origin that can multiply and “hop” to new sites in the genome, causing mutation. www.fhcrc.org /pubs/center_news/2003/jul3/sart1.html   (1237 words)

Proposed Genetic Nomenclature Rules for Tetrahymena thermophila, Paramecium primaurelia and Paramecium tetraurelia -- ... The genetics of the ciliated protozoa Tetrahymena thermophila Tetrahymena and Paramecium are unicellular eukaryotes with various The nuclear apparatus of Tetrahymena and Paramecium is composed www.genetics.org /cgi/content/full/149/1/459   (1770 words)

Web Site - Tetrahymena UIUC The Nanney laboratory was dedicated to the study of the genetics of ciliated protozoa of the genus Tetrahymena. The development of tetrahymena into the laboratory instrument that has become useful in the analysis of eukaryotic epigenetics, began at the University of Michigan with an identification of genetic markers, an analysis of macronuclear assortment, and an intensive inbreeding program to produce vigorous homozygous strains. The successful funding strategy for the Illinois tetrahymena program was to argue the advantages of a ciliate organismic technology as a tool in comparative genetics, but many bootlegged experiments were frankly exploratory and contributed little to the development of the sophisticated analytical instrument that T. thermophila became. www.life.uiuc.edu /nanney/website.html   (903 words)

G-overhang dynamics at Tetrahymena telomeres -- Jacob et al. 20 (15): 4299 -- The EMBO Journal   (Site not responding. Last check: 2007-10-22) Blackburn,E.H. and Gall,J.G. (1978) A tandemly repeated sequence at the termini of the extrachromosomal ribosomal RNA genes in Tetrahymena. Brehm,S.L. and Cech,T.R. (1983) Fate of an intervening sequence ribonucleic acid: excision and cyclization of the Tetrahymena ribosomal ribonucleic acid intervening sequence in vivo. Collins,K. and Greider,C.W. Tetrahymena telomerase catalyzes nucleolytic cleavage and nonprocessive elongation. embojournal.npgjournals.com /cgi/content/full/20/15/4299   (6282 words)

TETRAHYMENA Tetrahymena is an oligohymenophoran ciliate and a very common freshwater ciliate. The oral apparatus is located on the anterior end of the cell and can be easily seen as a small cavity in the ventral view of the cell. These are a drawings demonstrating ventral and dorsal view of Tetrahymena vorax and Tetrahymena pyriformis under the light microscope with the 100x lens. www.zoology.ubc.ca /courses/bio332/Labs/CiliateProject/tetrahymena/TETRAweb.htm   (435 words)

Tetrahymena Macronuclear Genome Mapping: Colinearity of Macronuclear Coassortment Groups and the Micronuclear Map on ... Tetrahymena Macronuclear Genome Mapping: Colinearity of Macronuclear Coassortment Groups and the Micronuclear Map on Chromosome 1L -- Wickert et al. The genetics of Tetrahymena are enriched by the possession of , 1982  Self-splicing RNA: autoexcision and autocyclization of the ribosomal RNA intervening sequences of Tetrahymena. www.genetics.org /cgi/content/full/154/3/1155   (6418 words)

RNA Polymerase II Localizes in Tetrahymena thermophila Meiotic Micronuclei When Micronuclear Transcription Associated ... Rpc5p was identified in the Tetrahymena genome database. Isolation of micro- and macronuclei of Tetrahymena pyriformis. Martindale, D. A conjugation-specific gene (cnjC) from Tetrahymena encodes a protein homologous to yeast RNA polymerase subunits (RPB3, RPC40) and similar to a portion of the prokaryotic RNA polymerase alpha subunit (rpoA). ec.asm.org /cgi/content/full/3/5/1233   (4375 words)

Mapping the Germ-Line and Somatic Genomes of a Ciliated Protozoan, Tetrahymena thermophila -- Orias 8 (2): 91 -- ... Paramecium and Tetrahymena in one branch, and Euplotes, Oxytricha, Tetrahymena thermophila is the only ciliate in which a systematic genetic mapping of DNA polymorphisms has begun (Orias 1997 Tetrahymena is one of very few eukaryotes with which the powers of molecular biology, conventional genetics, and microbial www.genome.org /cgi/content/full/8/2/91   (3532 words)

Enhanced Survival of Salmonella enterica in Vesicles Released by a Soilborne Tetrahymena Species -- Brandl et al. 71 ... Tetrahymena was isolated from soil in California, and the isolate enterica serovar Thompson pGT-Kan in vesicles released by Tetrahymena SSU ( Each value represents the mean and standard error of the mean of the proportion of viable serovar Thompson cells over the total number of cells per vesicle or per area around the vesicle for 100 observations under the epifluorescence microscope. aem.asm.org /cgi/content/full/71/3/1562?etoc   (4350 words)

Methylation of adenine in the nuclear DNA of Tetrahymena is internucleosomal and independent of histone H1 -- Karrer ... Gorovsky,M.A., Hattman,D. and Pleger,G.L. ]methyladenine in the nuclear DNA of a eucaryote, Tetrahymena pyriformis. Harrison,G.S., Findly,R.C. and Karrer,K.M. (1986) Site-specific methylation of adenine in the nuclear genome of a eucaryote, Tetrahymena thermophila. Karrer,K.M. and Stein-Gavens,S. (1990) Constancy of adenine methylation in Tetrahymena macronuclear DNA. nar.oxfordjournals.org /cgi/content/full/30/6/1364   (4665 words)

15N Investigation into the Effect of a Pollutant on the Nitrogen Metabolism of Tetrahymena pyriformis as a Model for ... Tetrahymena, a typical eukaryotic cell, and mammalian cells have much in common in regard to their nutritional requirements, cell compartmentation, metabolic pathways, and sensitivity to cytotoxic substances (1-5). Tetrahymena pyriformis strain W (1630/1W) was obtained from the Culture Collection of Algae and Protozoa (CCAP; Ambleside, UK). With Tetrahymena pyriformis, conventional toxicity tests are usual and are performed using certain complex media (6,10-13). ehp.niehs.nih.gov /members/1998/106p493-497arndt/arndt-full.html   (3694 words)

[No title] Tetrahymena sp./ FITC-labeled yeast to each of their labeled tubes (containing 25 ml formalin) at the specified time. To find out which populations are the Tetrahymena cells, which are the FITC-labeled yeast cells and which are Tetrahymena which have FITC-labeled yeast cells in them, we can paint each population. In this case we want to know how many of the Tetrahymena cells contain FITC-labeled yeast without regard to debris or the FITC-labeled yeast cells which are present but have not been phagocytized by the Tetrahymena. www.sjsu.edu /depts/Biology/specialprogs/flocyto/html/fc-p05.html   (1268 words)

Tetrahymena Biogeography   (Site not responding. Last check: 2007-10-22) An evolutionary tree for tetrahymena species based on nucleic sequence of the D2 region of the large ribosomal RNA molecule (Nanney et al., 1998). Our information concerning the biogeography of strains of tetrahymena, and their genetic and evolutionary relationships, is based primarily on fresh water collections made by Elliott’s laboratory in Central and South America, Australia and the Pacific Islands, and to Europe (Elliott, 1973), and by the Nanney laboratory (Nyberg, 1981; Simon et al., 1985 and unpublished). This observation alone indicates that tetrahymena cryptic species, at least in their dispersive phase, may be sympatric. www.life.uiuc.edu /nanney/biogeography.html   (3539 words)

The First Life (Addendum C to Review of David Foster's The Philosophical Scientists) B.L. Bass and T.R. Cech, "Specific Interaction Between Self-Splicing of RNA of Tetrahymena and Its Guanosine Substrate," in Nature 308 (1984), p. 820; T.R. Cech, et al, "In Vitro Splicing of the Ribosomal RNA Precursor of Tetrahymena" in Cell 27 (1981), p. It is not yet known whether the tetrahymena was the basis of earth's protobiology. www.infidels.org /library/modern/richard_carrier/addendaC.html   (547 words)

Tetrahymena as Fish Food   (Site not responding. Last check: 2007-10-22) Tetrahymena has two advantages, namely that the culture medium contains only few components and is easy to prepare, and that sterile cultures are commercially available from the Culture Collection of Algae and Protozoa, Freshwater Biological Association, The Ferry House, Ambleside, Cumbria LA22 0LP, UK; phone 44-5394-42468. The strain we use is Tetrahymena pyriformis (catalogue no. CCAP 1630/1W), and the PPY culture medium is prepared as follows: Dissolve 20 g proteose peptone and 2.5 g yeast extract in 1000 ml of distilled water, and sterilize in batches of 200 ml in 500 ml bottles. Using Tetrahymena, we have been able to grow babyfish with survival rates comparable to those obtained with Paramecium. zebra.biol.sc.edu /methods/tetra.html   (286 words)

Tetrahymena Fimbrin Localized in the Division Furrow Bundles Actin Filaments in a Calcium-Independent Manner -- ... Tetrahymena Fimbrin Localized in the Division Furrow Bundles Actin Filaments in a Calcium-Independent Manner -- Shirayama and Numata 134 (4): 591 -- Journal of Biochemistry Tetrahymena Fimbrin Localized in the Division Furrow Bundles Actin Filaments in a Calcium-Independent Manner molecule of Tetrahymena fimbrin to 7 molecules of actin, F-actin jb.oxfordjournals.org /cgi/content/abstract/134/4/591   (193 words)

Tetrahymena: The key to the genetic analysis of the regulated pathway of polypeptide secretion? -- Hutton 94 (20): ... Tetrahymena: The key to the genetic analysis of the regulated pathway of polypeptide secretion? Tetrahymena: The key to the genetic analysis of the regulated pathway of polypeptide secretion? The potential of using Tetrahymena to investigate the molecular components of the regulated pathway of protein secretion and www.pnas.org /cgi/content/full/94/20/10490   (2272 words)

Flanking Regulatory Sequences of the Tetrahymena R Deletion Element Determine the Boundaries of DNA Rearrangement -- ... Flanking Regulatory Sequences of the Tetrahymena R Deletion Element Determine the Boundaries of DNA Rearrangement -- Chalker et al. Flanking Regulatory Sequences of the Tetrahymena R Deletion Element Determine the Boundaries of DNA Rearrangement Gershan, J. A., Karrer, K. A family of developmentally excised DNA elements in Tetrahymena is under selective pressure to maintain an open reading frame encoding an integrase-like protein. mcb.asm.org /cgi/content/abstract/19/8/5631   (654 words)

Developmentally Regulated Rpd3p Homolog Specific to the Transcriptionally Active Macronucleus of Vegetative Tetrahymena ... Identification and purification of young macronuclear anlagen from conjugating cells of Tetrahymena thermophila. Regulation of histone acetylation during macronuclear differentiation in Tetrahymena: evidence for control at the level of acetylation and deacetylation. Micronuclei and the cytoplasm of growing Tetrahymena contain a histone acetylase activity which is highly specific for free histone H4. mcb.asm.org /cgi/content/full/20/22/8319   (5549 words)

Genetics The species now called Tetrahymena thermophila was first considered to be Tetrahymena pyriformis. Unlike Paramecium, there is no programmed somatic (vegetative) senescence and death: Tetrahymena cell lines are in practice immortal. Genetic differences in Tetrahymena are mapped to chromosome arms by using nullisomic strains. www.lifesci.ucsb.edu /%7Egenome/Tetrahymena/genetics.htm   (7567 words)

Flanking Regulatory Sequences of the Tetrahymena R Deletion Element Determine the Boundaries of DNA Rearrangement -- ... A germ line-specific sequence element in an intron in Tetrahymena thermophila. Alternate junctions and microheterogeneity of Tlr1, a developmentally regulated DNA rearrangement in Tetrahymena thermophila. Transformation of Tetrahymena thermophila by microinjection of ribosomal RNA genes. mcb.asm.org /cgi/content/full/19/8/5631   (7442 words)

CELL BIOLOGY: ON TETRAHYMENA Hunger induces Tetrahymena cells to undergo a transformation, including growth of a long posterior cilium and more coordinated propulsion. In Tetrahymena, the diploid micronucleus is the germline. 4) Tetrahymena has provided many insights about cilia, including the discovery of dynein, elucidation of the cellular principles of self-templated cortical patterning and genetic and cytological analysis of tubulins and tubulin post-translational modifications. scienceweek.com /2005/sw050617-3.htm   (1472 words)

Intracellular folding of the Tetrahymena group I intron depends on exon sequence and promoter choice -- KODUVAYUR and ... Brehm, S.L. and Cech, T.R. Fate of an intervening sequence ribonucleic acid: excision and cyclization of the Tetrahymena ribosomal ribonucleic acid intervening sequence in vivo. Brion, P., Schroeder, R., Michel, F., and Westhof, E. Influence of specific mutations on the thermal stability of the td group I intron in vitro and on its splicing efficiency in vivo: a comparative study. Facilitation of group I splicing in vivo: misfolding of the Tetrahymena IVS and the role of ribosomal RNA exons. www.rnajournal.org /cgi/content/full/10/10/1526   (3365 words)

Gene Knockouts Reveal Separate Functions for Two Cytoplasmic Dyneins in Tetrahymena thermophila -- Lee et al. 10 (3): ... Batz, W., and Wunderlich, F. Structural transformation of the phagosomal membrane in Tetrahymena cells endocytosing latex beads. LaFountain, J.R., Jr., and Davidson, L.A. An analysis of spindle ultrastructure during prometaphase and metaphase of micronuclear division in Tetrahymena. Rosenbaum, J.L., and Carlson, K. Cilia regeneration in Tetrahymena and its inhibitions by colchicine. www.molbiolcell.org /cgi/content/full/10/3/771   (7092 words)

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