
	Where results make sense

Topic: Thermostability

In the News (Thu 31 Dec 09)

Northern Trust

Marvin Harrison

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Mickey Rourke

AR Rahman

Danny Boyle

Hugh Jackman

	Thermostability of GDH
		Trends commonly associated with elevated thermostability in proteins include relatively small solvent-exposed surface area, increased packing density that reduces cavities in the hydrophobic core, optimization of hydrophobic interactions, decreased length of surface loops, and hydrogen bonds between polar residues.
		The results of DSM and thermostability measurements of the wild type and mutant GluDHs are summarized in Table 2 and Fig 2.
		Thermostability and Tm of native P.furiosus and recombinant wt and mutant T.litoralis GluDHs.
	comb5-156.umbi.umd.edu /poster/gdh (1960 words)

	NETZSCH - Leading Thermal Analysis. - Branches / Industries
		Being cognizant of heat dissipation is therefore increasingly important.
		Certain thermoanalytical instruments can provide information concerning the thermostability and curing behavior of reactive resin systems even online!
		For metals and metal alloys, it is of practical importance to be aware of the specific heat and phase transformations, also under oxidative or corrosive atmosphere.
	www.4netzsch.com /en/branches (348 words)

	Prediction of Factors Determining Changes in Thermostability in Protein Mutants. (Site not responding. Last check: 2007-11-02)
		The prediction of protein thermostability is a key research area in protein structural bioinformatics.
		Besides, thermostable enzymes and proteins are needed for the industrial applications towards the improvement of chemical reactions.
		In this study, we have efficiently implemented and optimised the synergy between mean force potentials and structural data to predict the changes in thermostability in protein mutants.
	www.iscb.org /ismb2004/posters/parthiATuni-koeln.de_356.html (431 words)

	Project (Site not responding. Last check: 2007-11-02)
		This project focuses on protein thermostability and is intended to discuss the factors involved in protein thermostability recognized in hyperthermophiles, so far.
		The notion that increased thermostability results from an amalgam of many small changes throughout the enzyme is the basis for analyses such as calculating the total number of salt links, H-bonds, buried surface area, and surface to volume ratio.
		One of the effects assumed to contribute to its anomalous thermostability is the tendency of the tetrameric enzyme to form octamers (Jaenicke, 1996).
	www.cryst.bbk.ac.uk /pps97/assignments/projects/gazerro/project.htm (6940 words)

	Crystal Structure of the Hyperthermophilic Inorganic Pyrophosphatase from the Archaeon Pyrococcus horikoshii -- Liu et ...
		thermostability of Pho-PPase by stabilizing the C-terminus and
		Cation-induced thermostability of yeast and Escherichia coli pyrophosphatases.
		Determinants of protein thermostability observed in the 1.9-A crystal structure of malate dehydrogenase from the thermophilic bacterium Thermus flavus.
	www.biophysj.org /cgi/content/full/86/1/420 (3663 words)

	A Comparison of the Thermostability of Glyceraldehyde 3-phosphate Dehydrogenase From Thermophiles and Mesophiles in ... (Site not responding. Last check: 2007-11-02)
		The thermostability of D-glyceraldehyde 3-phosphate dehydrogenase (GAPDH) from two mesophilic sources was compared to that of GAPDH isolated from two thermophilic microorganisms.
		Activity measurements indicated the presence of thermostable GAPDH in the supernatant, which was subsequently applied to a chromatofocusing column (Sluyterman and Wijdenes 1981) for further purification.
		(1973) Purification by affinity chromatography of thermostable glyceraldehyde 3-phosphate dehydrogenase from Thermus aquaticus.
	www.jyi.org /volumes/volume6/issue1/articles/kaeberlein.html (4222 words)

	Poster Abstracts- Second International Conference on Protein Stabilisation
		The aim of the present study was to investigate effects of pH on activity, thermostability, and aggregation of GI provided by Novo Nordisk A/S. Protein concentration in the activity measurements varied from 0.01µM to 0.03µM GI, 2mM MgSO
		Thermostability and aggregation was investigated at 1.5µM GI, with 2mM MgSO
		From being inactive at pH 5.0, activity increased at both investigated temperatures up to pH 8.0 at 45°C and pH 8.5 - 9.0 at 60°C. Thermostability was investigated in the same pH range by circular dichroism; temperature was scanned from 5°C to 100°C with a scan rate at 1.5°C/min, response was measured at 223nm.
	www.ejbiotechnology.info /feedback/proceedings/04/poster/p13.html (335 words)

	Jahrbuch-CD der MPG 2003 - Engineering the thermostability
		It is also far more thermostable than the alpha-amylases from B. stearothermophilus and B. amyloliquefaciens despite the strong sequence similarities between these three proteins.
		Once a detailed X- ray structure of BLA was solved, structure-based mutagenesis was used to probe the role of residues involved in salt- bridges, calcium-binding or potential deamidation processes.
		Although a full understanding of the origin of BLA natural thermoresistance has not yet been reached, our study demonstrated that it is not optimized and that it can be increased or decreased artificially by several means.
	www.mpg.de /forschungsergebnisse/wissVeroeffentlichungen/archivListenJahrbuch/2002/75/publZIM197.html (353 words)

	Title of Invention: Protease with low thermostabili ty derived from Mucor pusillus
		The selection of a mutant strain producing an enzyme with low thermostability can be carried out by subjecting the culture broth of the mutagenized microorganism to thermal treatment as it is or after it is concentrated, and comparing the milk clotting activity prior to thermal treatment with the activity after such treatment.
		By using this procedure, a mutant strain producing a mutant enzyme with thermostability far lower than the thermostability of the MR produced by the original strain may be selected.
		The protease with low thermostability in accordance with the present invention can be obtained by isolating a mutant protease gene from the mutant strain selected in the manner described above, and expressing the gene or a further modified gene in other microorganisms.
	www.nal.usda.gov /bic/Biotech_Patents/1994patents/05332668.html (8897 words)

	Title of Invention: Construction of thermostable mu tants of a low molecular mass xylanase
		The thermostability of the 20,396 dalton Bacillus circulans xylanase was increased by site-directed mutagenesis.
		Therefore, the intrinsic thermostability of the enzymes is a critical parameter for their use in the bio-bleaching processes.
		Their improvements in thermostability are minor in comparison to that of the present invention.
	www.nal.usda.gov /bic/Biotech_Patents/1995patents/05405769.html (3260 words)

	Simultaneous Enhancement of Thermostability and Catalytic Activity of Phospholipase A1 by Evolutionary Molecular ...
		Thermostability is often a primary goal when workers try to improve the properties of an industrial enzyme, since high temperatures
		Thermostable mutants isolated from the first round of mutagenesis and screening (S1 through S8) produced transparent halos on the assay gel.
		Hao, J., Berry, A. A thermostable variant of fructose bisphosphate aldolase constructed by directed evolution also shows increased stability in organic solvents.
	aem.asm.org /cgi/content/full/66/3/890 (3445 words)

	World Intellectual Property Organization (Site not responding. Last check: 2007-11-02)
		Comparison with cellulases from the two mesophiles allows the identification of features potentially conferring thermostability, whilst a comparison with the structures of the thermostable family 11 xylanases gives an indication of the prevalence of the proposed thermostability features within the GH-C clan.
		It is yet a further object of the invention to provide for an variant clan C glycosyl hydrolase such as a family 12 glycosyl hydrolase or a related enzyme, wherein one or more amino acids are exchanged, added or deleted in order to change properties of the enzyme.
		The molecular basis for thermal stability, as demonstrated by comparing a thermostable protein to a homologous thermolabile protein, resides in the cumulative effect of variations in the amino acid sequence.
	www.wipo.int /ipdl/IPDL-CIMAGES/view/pct/getbykey5?KEY=03/89633.031030&ELEMENT_SET=DECL (7615 words)

	LABPV Newsletter issue 15
		It is shown in in vitro experiments that the activity and thermostability of POD and catalase from young pea plants (Bakardjieva and Christova, 1997), moss Mnium affine (Bakardjieva et al.
		At 20° C their activity was not influenced by the effectors applied but at 60° C in the presence of Ca and aminoacids a greater thermostability was observed.
		The thermostability of the two enzymes and POD isoforms is changed sometimes significantly.
	www.unige.ch /LABPV/newsletters/newsl15/n15p69.html (2029 words)

	FEBS Abstract
		The final thermostability of either EF-Tu was found to be the result of a cooperative interaction between the G-domain and domains 2+3.
		The G-domain set up the ‘‘basic’’ level of the thermostability, which was about 20 °C higher for the BstG-domain than for the EcG-domain in correlation with the growth temperature optimum difference between both bacteria.
		Two distinct structural features were found to correlate with the higher thermostability of the BstG-domain: an increase of charged residues at the expense of polar uncharged residues (CvP bias), and a decrease in the nonpolar solvent accessible surface area.
	www.blackwellpublishing.com /febsabstracts2004/abstract.asp?id=18144 (389 words)

	ARS \| Publication request: The Effect of Adding and Removing N-Glycosylation Recognition Sites on the Thermostability ...
		Interpretive Summary: The thermostability of malt carbohydrases is important because the conversion of starch to fermentable sugars during mashing typically takes place at temperatures of 60-75 degrees Celsius.
		Our strategy for selection of mutagenesis targets involved comparing the deduced amino acid sequences of alpha-glucosidases from two plant species and the thermostability of the enzymes encoded, followed by identification of key amino acids that were present in the thermostable enzymes but absent in the barley alpha-glucosidase.
		We identified NGRS present in the thermostable sugar beet enzyme that were absent in the enzyme from barley.
	www.ars.usda.gov /research/publications/publications.htm?SEQ_NO_115=157554 (540 words)

	Hyperthermophilic Enzymes: Sources, Uses, and Molecular Mechanisms for Thermostability -- Vieille and Zeikus 65 (1): 1 ...
		thermostability, and the upper temperature limit for enzyme function.
		Thermostability and optimal activity at high temperatures are inherent properties of hyperthermophilic enzymes.
		to be necessary for optimal kinetic thermostability (365).
	mmbr.asm.org /cgi/content/full/65/1/1?view=full&pmid=11238984 (7622 words)

	KFtr-Paper (Site not responding. Last check: 2007-11-02)
		The salt dependent induction of oligomerization was parallelled by an increase in thermostability as determined by differential scanning calorimetry and heat inactivation.
		Finally, the thermostability of the enzyme at different salt concentrations was determined by differential scanning calorimetry and the excess heat capacity curves obtained correlated with the kinetics of heat inactivation of the enzyme under the same conditions.
		The increased thermostability is, however, at least in part due also to the induction of oligomerization by lyotropic salts.
	www.biophys.uni-frankfurt.de /tziatzios/KFTR-PAPER/KFtr-Paper.html (4745 words)

	[No title] (Site not responding. Last check: 2007-11-02)
		Our proposal focuses on two key attributes of molecular evolution; firstly, what are the characteristics of bacterial populations as they adapt to environmental extremes, and secondly, what are the physicochemical properties of the proteins generated from these selections.
		The goals of this work are to elucidate the pathways used in vivo and to apply biophysical methods to understand the physical basis for the mutational pathways we have elaborated as they relate to molecular evolution in all organisms.
		We will then be in a position to directly test the additive or cooperative nature of mutations by looking at a subset of mutations that we know already result in thermostability and are related to one another in evolutionary space.
	www-bioc.rice.edu /~shamoo/counago.html (340 words)

	Practical application of modelling and analysis techniques
		Improving the thermostability of the neutral protease of Bacillus stearothermophilus by replacing a buried asparagine by leucine.
		Increasing the thermostability of the neutral protease of Bacillus Stearothermophilus by improvement of internal hydrogen bonding.
		The effect of cavity-filling mutations on the thermostability of Bacillus stearothermophilus neutral protease.
	swift.cmbi.ru.nl /gv/articles/ref2.html (2079 words)

	Prediction of Protein Thermostability (Site not responding. Last check: 2007-11-02)
		The understanding of the factors governing protein stability is of outmost importance for a variety of research fields in biochemistry.
		In addition to a detailed understanding of protein folding it allows the design of prote ins with increased thermostability.
		Whereas a number of successful em pirical strategies for the design of stabilized proteins can be found in the literature, most of the designs were done by "manual design" compared to an automatic calculation of stabilizing protein sequence variations that should be theoretically possible as far as only small changes in the 3D structure occur.
	www.bioinfo.de /isb/gcb99/poster/leven (244 words)

	Two exposed amino acid residues confer thermostability on a cold shock protein - Nature Structural & Molecular ... (Site not responding. Last check: 2007-11-02)
		To understand protein thermostability at the molecular level we studied a pair of cold shock proteins, one of mesophilic and one of thermophilic origin, by systematic mutagenesis.
		The mesophilic protein could be converted to a highly thermostable form by changing the Glu residues at positions 3 and 66 to Arg and Leu, respectively.
		The molecular determinants of their high thermostability are, however, not well understood.
	www.nature.com /cgi-taf/DynaPage.taf?file=/nsmb/journal/v7/n5/full/nsb0500_380.html (2792 words)

	WSU PhD in Materials Science Program (Site not responding. Last check: 2007-11-02)
		Thermostability: Compared to their mesophilic counterparts, thermophilic enzymes have a few kcal/mol additional stabilization energy.
		The origins of this thermostability should lie within interactions between the constituent amino acid residues and solvent.
		To study the factors leading to stabilization, we are comparing the detailed structure and thermostability of a number of systematically designed chimeric recombinant proteins from a mesophilic and a thermophilic organism.
	www.wsu.edu /~matscipr/kang.html (746 words)

	Silent DNA: speaking the RNA language?
		The revealed effect was not due to correlation of RNA/RNA and RNA/DNA thermostabilities with thermostability of the DNA/DNA duplex, which, on the contrary, was lower than in the randomized sequence and lagged behind the elevation of GC content.
		In the CDS, the relative thermostabilities of RNA/RNA and RNA/DNA duplexes were also positive in the genomes of warm-blooded vertebrates and increased with elevation of GC content, whereas the relative thermostabilities of DNA/DNA duplex showed the opposite trend (Table 1).
		The lagging thermostability of DNA/DNA duplex is understandable because the melting energy of the long DNA duplexes is much higher than the body temperature of warm-blooded vertebrates (Sugimoto et al.
	www.euchromatin.org /Vinogradov01.htm (1804 words)

	Orals - Second International Conference on Protein Stabilisation
		Phytase is used as an animal feed supplement to enhance the nutritive value of plant material by liberation of inorganic phosphate from phytic acid (myo-inositol hexakisphosphate) and, thereby, to reduce environmental phosphorus pollution.
		For feed application a thermostable phytase is of general interest to circumvent problems that may occur during the formulation and feed pelleting process where temporarily high temperatures (80-100 °C) and shear stress may affect protein structure and lead to activity loss.
		The disodium salts of malonic and succinic acid led to a concentration-dependent increase in A. fumigatus and A. nidulans phytase thermostability and to stimulation of their enzymatic activity.
	www.ejbiotechnology.info /feedback/proceedings/04/oral/b6.html (372 words)

	Thermostability of mouse-passaged BSE and scrapie is independent of host PrP genotype: implications for the nature of ...
		Thermostability of mouse-passaged BSE and scrapie is independent of host PrP genotype: implications for the nature of the causal agents
		Comparison of dose-response curves (a) and lesion profiles (b) of autoclaved and unheated agents in mouse-passaged BSE and scrapie brain macerates.
		Taylor, D. M., Fernie, K., McConnell, I. and Steele, P. Observations on thermostable subpopulations of the unconventional agents that cause transmissible degenerative encephalopathies.
	vir.sgmjournals.org /cgi/content/full/83/12/3199 (2726 words)

	Understanding thermostability in cytochrome P450 by combinatorial mutagenesis -- Maves and Sligar 10 (1): 161 -- ...
		thermostability in the GAPDH family (Tanner et al.
		Rhode, D.J. and Martin, B.L. Localized structural effects of electrostatic interactions in a thermostable enzyme.
		Tanner, J.J., Hecht, R.M., and Krause, K.L. Determinants of enzyme thermostability observed in the molecular structure of Thermus aquaticus D-Glyceraldehyde-3-phosphate dehydrogenase at 2.5 A resolution.
	www.proteinscience.org /cgi/content/full/10/1/161 (4271 words)

	An Integrated Structural and Computational Study of the Thermostability of Two Thioredoxin Mutants from ...
		the observed greater thermostability of the mutant form (35).
		Engineering a thermostable protein via optimization of charge-charge interactions on the protein surface.
		Two exposed amino acid residues confer thermostability on a cold shock protein.
	jb.asm.org /cgi/content/full/185/14/4285 (2561 words)

	Homology modelling of GAPDH from Thermotoga (Site not responding. Last check: 2007-11-02)
		The model was compared with the structure of Bacillus stearothermophilus GAPDH to identify structural details underlying the increased thermostability.
		This seems to be the most important factor responsible for thermostability.
		The model predicts that a slight increase in alpha-helical propensities and helix-dipole interactions also contribute to increased stability, but to a lesser degree.
	www.enzim.hu /~szia/cikk1.html (220 words)

	Preferred amino acids and thermostability
		Most organisms grow at temperatures from 20 to 50°C, but some prokaryotes, including Archaea and Bacteria, are capable of withstanding higher temperatures, from 60 to >100°C. Their biomolecules, especially proteins, must be sufficiently stable to function under these extreme conditions; however, the basis for thermostability remains elusive.
		We investigated the preferential usage of certain groupings of amino acids and codons in thermally adapted organisms, by comparative proteome analysis, using 28 complete genomes from 18 mesophiles (M), 4 thermophiles (T), and 6 hyperthermophiles (HT).
		Analysis of codon usage revealed that HT had more AGR codons for Arg than they did CGN codons, which were more common in mesophiles.
	funpecrp.com.br /gmr/year2003/vol4-2/gmr0079_abstract.htm (260 words)

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