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Thermus - MicrobeWiki Thermus bacteria cells are a Gram-negative bacteria and are "either immotile or possess flagella and are motile" (R. Huber et al. Thermus is assumed to have a pilus-like structure for conjugation, though the molecular basis of the DNA uptake is still unknown. Thermus strains often use monosaccharides as a source for single carbons, though typically pentoses is not metabolized. microbewiki.kenyon.edu /index.php/Thermus   (616 words)

Thermus aquaticus - Wikipedia, the free encyclopedia Thermus aquaticus is a species of bacterium that can tolerate high temperatures; it is the source of the heat-resistant enzyme Taq DNA Polymerase, one of the most important enzymes in molecular biology because of its use in the polymerase chain reaction. Thermus aquaticus is one of several thermophilic bacteria that belong to the Deinococcus-Thermus group. Most molecular biologists probably became aware of Thermus aquaticus in the late 1970s or early 1980s because of the isolation of useful restriction endonucleases from this organism. en.wikipedia.org /wiki/Thermus_aquaticus   (1166 words)

Thermus HUDSON (J.A.), MORGAN (H.W.) and DANIEL (R.M.): Thermus filiformis sp. LOGINOVA (L.G.), EGOROVA (L.A.), GOLOVACHEVA (R.S.) and SEREGINA (L.M.): Thermus ruber sp. 1995 proposed the name Thermus thermophilus as valid only in the abstract of the publication without a formal proposal in the text, and did not present phenotypic results showing that the species could be distinguished from other species of the genus Thermus. www.bacterio.cict.fr /t/thermus.html   (1287 words)

Distribution of Genes for Synthesis of Trehalose and Mannosylglycerate in Thermus spp. and Direct Correlation of These ... Osmotic adaptation of Thermus thermophilus RQ-1: a lesson from a mutant deficient in the synthesis of trehalose. Trehalose biosynthesis in Thermus thermophilus RQ-1: biochemical properties of the trehalose-6-phosphate synthase and trehalose-6-phoshate phosphatase. Cloning and sequencing of trehalose synthase gene from Thermus aquaticus ATCC 33923. aem.asm.org /cgi/content/full/71/5/2460   (4161 words)

In hot waters Eventually, he found organisms that could live and reproduce near the temperature of boiling water -- 100 C. The prize of his collection was an organism he named Thermus aquaticus (for its affinity for hot water) and deposited in a public repository for study by other scientists. Thermus aquaticus in an outflow channel of a hot spring at Yellowstone. But more important, at least to scientists who don't guzzle fizzy pop at the lab bench, Thermus aquaticus supplied TAQ polymerase, the essential enzyme for polymerase chain reaction, AKA PCR. whyfiles.org /022critters/hot_bact.html   (583 words)

Organization and Expression of a Thermus thermophilus Arginine Cluster: Presence of Unidentified Open Reading Frames ... For all experiments, Thermus was grown at 75°C in arginine- and uracil-free liquid medium (4) with 20 mM pyruvate as the arginine represses OTCase synthesis in Thermus (27, 31). Arginine biosynthesis and regulation in Lactobacillus plantarum: the carA gene and the argCJBDF cluster are divergently transcribed. jb.asm.org /cgi/content/full/182/20/5911   (2655 words)

Title of Invention: Purified thermostable enzyme The thermostable enzyme may be native or recombinant and may be used in a temperature-cycling chain reaction wherein at least one nucleic acid sequence is amplified in quantity from an existing sequence with the aid of selected primers and nucleotide triphosphates. Because there may not be a precisely exact match between the nucleotide sequence in the Thermus aquaticus form and that in the corresponding portion of other species, oligomers containing approximately 12-18 nucleotides (encoding the four to six amino acid stretch) are probably necessary to obtain hybridization under conditions of sufficient stringency to eliminate false positives. Whether the nucleic acid is single- or double-stranded, the DNA polymerase from Thermus aquaticus may be added at the denaturation step or when the temperature is being reduced to or is in the range for promoting hybridization. www.nal.usda.gov /bic/Biotech_Patents/1994patents/05352600.html   (18865 words)

ScienceDaily: Scientists Visualise Cellular Handmaiden That Restores Shape To Proteins The structure of the chaperonin complex of the bacteria Thermus thermophilus reveals clues about how the important molecule may do its job of folding new or damaged proteins within cells. Unlike the first structure, taken from the chaperonin of gut bacterium Escherichia coli, the Thermus thermophilus structure is a more natural structure revealing the irregular oval interior of the cage's subunits. Thermus thermophilus is a highly thermophilic bacteria, first found living in deep-sea hot vents. www.sciencedaily.com /releases/2004/08/040818085447.htm   (1630 words)

Life at High TemperaturesLife at High Temperatures The bacterium Thermus aquaticus was first discovered in several springs in the Great Fountain area of the Lower Geyser Basin. Here Thermus aquaticus is living on tiny amounts of organic matter present in the source water, making it visible to the naked eye. At the time of the discovery, this spring was hotter than it is today, and its outflow was 70 degrees C (158 degrees F), the optimum temperature of Thermus aquaticus. www.bact.wisc.edu /Bact303/b27   (1018 words)

Purification and Characterization of a Novel Thermo-Alkali-Stable Catalase from Thermus brockianus A novel thermo-alkali-stable catalase from Thermus brockianus was purified and characterized. Thermus brockianus was cultured to stationary phase at 70 The catalase showed a strong Soret peak at 410 nm and a peak at 534 nm with a shoulder occurring from 560 to 570 nm. pubs.acs.org /cgi-bin/sample.cgi/bipret/2003/19/i04/html/bp034040t.html   (6130 words)

New Host-Vector System for Thermus spp. Based on the Malate Dehydrogenase Gene -- Kayser and Kilbane 183 (5): 1792 -- ... A Thermus thermophilus HB27 strain was constructed in which the malate dehydrogenase (mdh) gene was deleted. Characterization of an operon encoding succinyl-CoA synthetase and malate dehydrogenase from Thermus flavus AT-62 and its expression in Escherichia coli. An operon encoding aspartokinase and purine phosphoribosyltransferase in Thermus flavus. jb.asm.org /cgi/content/full/183/5/1792   (1701 words)

The Microbiology of Thermus Aquaticus Three girls asked themselves a question, "I wonder if the bacteria, Thermus Aquaticus, found in our hot springs is the same as the Thermus found around the world." This question was extremely important because if the conclusion was that the bacteria was unique, it would be like witnessing evolution in process. Because of the extream conditions that the bacteria live in, their migration from one hotsprings to another would be nearly impossible. Thermus cells have to be lysed, and DNA from the cells must be purified and sequenced. www.cascadehs.csd.k12.id.us /advbio/99-00/thermus.html   (468 words)

McDonald 2001   (Site not responding. Last check: 2007-11-06) Asymmetrical patterns of amino acid substitution in proteins of organisms living at moderate and high temperatures (mesophiles and thermophiles, respectively) are generally taken to indicate selection favoring different amino acids at different temperatures due to their biochemical properties. Chemotaxonomic and molecular-genetic studies of the genus Thermus: evidence for a phylogenetic relationship of Thermus aquaticus and Thermus ruber to the genus Deinococcus. Halotolerant Thermus strains from marine and terrestrial hot springs belong to Thermus thermophilus (ex Oshima and Imahori, 1974) nom. udel.edu /~mcdonald/msmcd01.html   (4397 words)

[Analogs of nucleotides, modified by a sugar residue and pyrimidine base, in a DNA synthesis reaction, catalyzed by ... Substrate properties of dNTP analogues in the DNA synthesis reaction catalyzed by Thermus aquaticus DNA polymerase were studied. It was shown that most of dNTP analogues which were known as terminators of DNA synthesis of E. coli DNA polymerase I were able to terminate DNA synthesis catalyzed by Thermus aquaticus DNA polymerase. An interesting feature of Thermus aquaticus DNA polymerase was the ability to utilize 3'-azido-2',3'-dideoxythymidine triphosphate as terminating substrate. www.aegis.com /aidsline/1992/nov/M92B0324.html   (356 words)

An unusual mechanism of bacterial gene expression revealed for the RNase P protein of Thermus strains -- Feltens et al. ... An unusual mechanism of bacterial gene expression revealed for the RNase P protein of Thermus strains -- Feltens et al. We have investigated rnpA expression in thermophilic bacteria of the genus Thermus. The codon usage of rnpA is expected to be constrained in the region overlapping rpmH. www.pnas.org /cgi/content/full/100/10/5724   (3909 words)

DNA relatedness of Thermus strains, description of Thermus brockianus sp. nov., and proposal to reestablish Thermus ... DNA relatedness of Thermus strains, description of Thermus brockianus sp. nov., and proposal to reestablish Thermus thermophilus (Oshima and Imahori) [published erratum appears in Int J Syst Bacteriol 1996 Apr;46(2):629] -- Williams et al. nov., and proposal to reestablish Thermus thermophilus (Oshima and Imahori) [published erratum appears in Int J Syst Bacteriol 1996 Apr;46(2):629] ijs.sgmjournals.org /cgi/content/abstract/45/3/495   (350 words)

ATCC: Bacteria Collection Thermus thermophilus (Oshima and Imahori) Williams et al.; deposited as Thermus thermophilus Oshima and Imahori Isolation and characterization of an extremely thermostable D-xylose isomerase from Thermus aquaticus HB 8. A rapid procedure for purifying a restriction endonuclease from Thermus thermophilus (Tth I). www.atcc.org /common/catalog/numSearch/numResults.cfm?atccNum=27634   (513 words)

Purification and Characterization of the Recombinant Thermus sp. Strain T2 {alpha}-Galactosidase Expressed in ... Purification and Characterization of the Recombinant Thermus sp. The replacement of Cys by Ala of the enzyme was carried out to analyze the role of the enzyme's Cys residue. Purification and properties of a new alpha-galactosidase specified by a transmissible plasmid. aem.asm.org /cgi/content/full/67/4/1601   (2409 words)

Discover coffee maker thermus cup and coffee maker and grinder here.   (Site not responding. Last check: 2007-11-06) -Bring a thermus cup, great for coffee in the morning and cold drinks after that, the bigger the... was the smell of coffee waifing in the front seat as my parents often carried a thermus.... Mix Master: February 2005 the thermus of coffee is my only friend. www.coffeemachinecenter.com /coffee-makerthermus-cup.html   (547 words)

Cloning and characterization of tRNA (m1A58) methyltransferase (TrmI) from Thermus thermophilus HB27, a protein ... Cloning and characterization of tRNA (m1A58) methyltransferase (TrmI) from Thermus thermophilus HB27, a protein required for cell growth at extreme temperatures -- Droogmans et al. Thermus thermophilus (HB27) was grown at 70°C on a rotary Morozov,I.A., Gambaryan,A.S., Lvova,T.N., Nedospasov,A.A. and Venkstern,T.V. (1982) Purification and characterization of tRNA (adenine-1-)-methyltransferase from Thermus flavus strain 71. nar.oxfordjournals.org /cgi/content/full/31/8/2148   (4702 words)

Recombinant Cytochrome rC557 Obtained from Escherichia coli Cells Expressing a Truncated Thermus thermophilus cycA ... Recombinant Cytochrome rC557 Obtained from Escherichia coli Cells Expressing a Truncated Thermus thermophilus cycA Gene. Thermus, it was evident that the resulting product was actually From spontaneous maturation of "signal peptide-deficient" Thermus CycA in the cytoplasm of E. www.jbc.org /cgi/content/full/276/9/6537   (5103 words)

Thermus oshimai sp. nov., isolated from hot springs in Portugal, Iceland, and the Azores, and comment on the concept of ... nov., isolated from hot springs in Portugal, Iceland, and the Azores, and comment on the concept of a limited geographical distribution of Thermus species -- Williams et al. aquaticus, Thermus brockianus, and Thermus filiformis were not detected in We propose that the isolates that belonged to phenetic ijs.sgmjournals.org /cgi/content/abstract/46/2/403   (292 words)

UniProtKB/Swiss-Prot entry P19821 [DPO1_THEAQ] DNA polymerase I, thermostable Korolev S. Nayal M. Barnes W.M. di Cera E. Waksman G. "Crystal structure of the large fragment of Thermus aquaticus DNA polymerase I at 2.5-A resolution: structural basis for thermostability."; Li Y. Korolev S. Waksman G. "Crystal structures of open and closed forms of binary and ternary complexes of the large fragment of Thermus aquaticus DNA polymerase I: structural basis for nucleotide incorporation."; Murali R. Sharkey D.J. Daiss J.L. Krishna Murthy H.M. "Crystal structure of Taq DNA polymerase in complex with an inhibitory Fab: the Fab is directed against an intermediate in the helix-coil dynamics of the enzyme."; www.expasy.org /uniprot/P19821   (504 words)

www.myspace.com/thermusrocks I remember being in high school and pains-takingly cutting out plaid pieces of fabric to sew "Thermus" on my bookbag. Now instead of listening to Jeremy sit around and whine about how great his Thermus memories are, and how he'll never get those days back and how he blames himself for the split every night with a gun to his head, we can actually see a Thermus show! I remember when Bongs, Bullets and Booze was the theme song of a big group of us. www.myspace.com /thermusrocks   (920 words)

{alpha}-Aminoadipate aminotransferase from an extremely thermophilic bacterium, Thermus thermophilus -- Miyazaki et al. ... Hidaka, Y., Hasegawa, M., Nakahara, T. and Hoshino, T. The entire population of Thermus thermophilus cells is always competent at any growth phase. Miyazaki, J., Kobashi, N., Fujii, T., Nishiyama, M. and Yamane, H. Characterization of a lysK gene as an argE homolog in Thermus thermophilus HB27. Miyazaki, J., Kobashi, N., Nishiyama, M. and Yamane, H. Characterization of homoisocitrate dehydrogenase involved in lysine biosynthesis of an extremely thermophilic bacterium, Thermus thermophilus HB27, and evolutionary implication of beta-decarboxylating dehydrogenase. mic.sgmjournals.org /cgi/content/full/150/7/2327   (3629 words)

Thermus Aquaticus DNA Polymerase, Recombinant Printable version: [ Thermus Aquaticus DNA Polymerase, Recombinant ] Taq DNA Polymerase (recombinant form of the enzyme from the thermophilic eubacterium Thermus aquaticus strain YT-1) consists of a single polypeptide chain with a molecular weight 94 kDa. www.hytest.fi /tuotteet125.php   (206 words)

Identification and characterization of single-stranded-DNA-binding proteins from Thermus thermophilus and Thermus ... Identification and characterization of single-stranded-DNA-binding proteins from Thermus thermophilus and Thermus aquaticus - new arrangement of binding domains -- Dabrowski et al. Identification and characterization of single-stranded-DNA-binding proteins from Thermus thermophilus and Thermus aquaticus – new arrangement of binding domains Improvements of rolling circle amplification (RCA) efficiency and accuracy using Thermus thermophilus SSB mutant protein. mic.sgmjournals.org /cgi/content/abstract/148/10/3307   (515 words)

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