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Topic: Thin layer chromatography


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In the News (Tue 14 Feb 12)

  
  CHP - Thin-Layer Chromatography   (Site not responding. Last check: 2007-10-19)
Thin-layer chromatography (TLC) is a chromatographic technique that is useful for separating organic compounds.
Thin-layer chromatography consists of a stationary phase immobilized on a glass or plastic plate, and an organic solvent.
TLC can be automated using forced solvent flow, running the plate in an vacuum-capable chamber to dry the plate, and recording the finished chromatogram by absorption or fluorescence spectroscopy with a light source.
www.chem.vt.edu /chem-ed/sep/tlc/tlc.html   (339 words)

  
 Thin Layer Chromatography module
Thin layer chromatography (TLC) is a method for identifying substances and testing the purity of compounds.
TLC is a useful technique because it is relatively quick and requires small quantities of material.
The stationary phase is a thin layer of adsorbent (usually silica gel or alumina) coated on a plate.
uwplatt.edu /chemep/chem/chemscape/labdocs/catofp/chromato/tlc/tlc.htm   (0 words)

  
 Thin-layer chromatography of lipids
With TLC in the adsorption mode (silica gel), the principle application in lipid analysis is for the separation of different lipid classes from animal and plant tissues.
I can remember when silver ion TLC was viewed by industrial analysts as a rather esoteric technique, but it has come to be the standard method for the determination of cocoa butter equivalents in confectionery fats, for example.
Reversed-phase TLC has been relatively little used for the analysis of triacylglycerols, mainly because it has been considered a messy technique and because the separated components were not easily visualised.
www.lipidlibrary.co.uk /topics/tlc   (0 words)

  
 thin-layer chromatography
Thin-layer chromatography (TLC) is perhaps the most efficient and versatile technique ever discovered for separation of complex lipid mixtures as well as for individual lipids.
TLC is rapid since up to 10 spots may be run frequently in less than an hour, it does not require any special equipment and allows the characterization of the separated lipids with various staining reactions.
The sample is developed on a thin layer quartz rod "Chromarod", its components being detected in a second step by the Iatroscan automatically and quickly.
www.cyberlipid.org /fraction/frac0005.htm   (0 words)

  
 Chem 211 - Thin Layer Chromatography
Chromatography works on the principle that different compounds will have different solubilities and adsorption to the two phases between which they are to be partitioned.
Thin Layer Chromatography (TLC) is a solid-liquid technique in which the two phases are a solid (stationary phase) and a liquid (moving phase).
TLC involves spotting the sample to be analyzed near one end of a sheet of glass or plastic that is coated with a thin layer of an adsorbent.
www.wellesley.edu /Chemistry/chem211lab/Orgo_Lab_Manual/Appendix/Techniques/TLC/thin_layer_chrom.html   (0 words)

  
 Thin Layer Chromatography
Theory of chromatography uses the different polarities of the stationary phase, mobile phase and the liquid mixture of different compound to be separated.  The compounds that have the most similar polarity to the mobile phase i.e.
TLC (thin layer chromatography) can be an inexpensive screening test which is often used prior to more expensive analytical tests.
The separation of a mixture is based on a difference in the degree of attraction between the components and the stationary and mobile phases.  In thin layer chromatography, the stationary phase is the silica plate and the mobile phase is the solvent of water and acetone.
www.ncsu.edu /kenan/fellows/2002/pligon/pharm/labs/chromo.html   (0 words)

  
 BISC 429
Thin layer chromatography is based on the separation of a mixture of compounds as it migrates with the help of a suitable solvent through a thin layer of adsorbent material which has been applied to an appropriate support.
Following the development of a relatively simple apparatus for preparing chromatoplates with uniformly thin layers of adsorbent by Stahl (1964), TLC became very popular.
In adsorption TLC the sample is continually fractionated as it migrates through the adsorbent layer.
www.sfu.ca /bisc/bisc-429/TLC.html   (0 words)

  
 THIN LAYER CHROMATOGRAPHY
The background theory of chromatography in general and thin-layer chromatography in specific is adequately explained in the reading you have done in Zubrick.
In TLC the sample is initially dissolved in an easily evaporated solvent and applied to a plate coated with the stationary phase using an applicator.
Chromatography originally got its name from the Greek word "chromatokis" meaning "suited for color" because early work involved the separation of colored pigments.
www.muhlenberg.edu /depts/chemistry/chem201woh/TLCF01.html   (0 words)

  
 Chromatography - Wikipedia, the free encyclopedia
Chromatography (from Greek χρώμα: chroma, colour) is the collective term for a family of laboratory techniques for the separation of mixtures.
Chromatography is a separation method that exploits the differences in partitioning behavior between a mobile phase and a stationary phase to separate the components in a mixture.
Thin layer chromatography (TLC) is a widely-employed laboratory technique and is similar to paper chromatography.
en.wikipedia.org /wiki/Chromatography   (0 words)

  
 Thin layer chromatography - Wikipedia, the free encyclopedia
Thin layer chromatography (TLC) is a widely-used chromatography technique used to separate chemical compounds.
It involves a stationary phase consisting of a thin layer of adsorbent material, usually silica gel, alumina, or cellulose immobilised onto a flat, inert carrier sheet.
TLC plates are made by mixing the adsorbent, such as silica gel, with a small amount of inert binder like calcium sulfate (gypsum) and water.
en.wikipedia.org /wiki/Thin_layer_chromatography   (0 words)

  
 Thin-Layer Chromatography
Chromatography is a word used to encompass a range of techniques in which mixtures of pure substances are separated into the individual substances by using a mobile phase (usually a liquid or gas) to push the mixture along a stationary phase (usually a solid or liquid coated on a solid).
Thin-Layer Chromatography (TLC) is a simple and inexpensive technique that is often used to judge the purity of a synthesized compound or to indicate the extent of progress of a chemical reaction.
Before attempting to apply TLC to the challenging problem of separating and identifying amino acids, it is advisable to learn and practice the technique by applying it to mixtures that are easily visualized and separated.
www.wpi.edu /Academics/Depts/Chemistry/Courses/General/tlc.html   (0 words)

  
 Thin-Layer Chromatography
The stationary phase is applied to a glass or plastic (usually polyester) sheet in a layer about 250 µm thick.
Spots are visualized as with paper chromatography, with one addition: silica gel sheets may have an adsorbed fluorescent dye.
Reports/procedures should have the same data as for paper chromatography, plus specification of the plate coating (usually cellulose or silica; perhaps alumina or magnesium silicate ("Florisil"); perhaps the particle size, and any fluorescent indicator).
www.dyerlabs.com /chemistry/thinlayer.html   (0 words)

  
 [No title]
Thin layer chromatography utilizes a thin film of silica coated onto a glass or plastic strip.
A mixture of the compounds to be separated is placed in a small spot at one end of a strip, and a liquid organic solvent (mobile phase) is passed over the spot.
Thin Layer Chromatography Sheets - you should cut the sheets with a paper cutter to a size appropriate for the number of spots you have.
www.valpo.edu /organization/psme/labs/tlc/Lipstick.doc   (0 words)

  
 Principles and Practice of Chromatography: TLC
Thin layer chromatography appears to have been first developed and utilized by Schraiber in 1939 (11).
" It occurred to us that a thin layer of the sorbent could be used in lieu of a strip of paper; also we felt that the flat bed could be considered as a cut-out of the adsorbent column.
In our work, we deposited a drop of the solution being investigated on the flat adsorbent layer and observed the separation into concentric circular zones which could become visible because of their fluorescence in the light of a UV lamp.
www.chromatography-online.org /Principles/TLC/rs61.html   (0 words)

  
 European Mycotoxin Awareness Network - EMAN - Thin Layer Chromatography   (Site not responding. Last check: 2007-10-19)
Thin-layer chromatography (TLC) is a chromatographic technique that is useful for the separation, purity assessment and identification of organic compounds.
Thin-layer chromatography consists of a stationary phase immobilised on a glass or plastic plate and a solvent as mobile phase.
The chromatography chamber is closed and therefore saturated with mobile phase for stable conditions.
www.lfra.co.uk /eman2/fsheet2_10.asp   (0 words)

  
 Friends Lab - Methodologies [Thin Layer Chromatography] (via CobWeb/3.1 planetlab2.isi.jhu.edu)   (Site not responding. Last check: 2007-10-19)
TLC is based on the separation of constituents (drugs) by the elution of solvents on a thin layer of absorbent material coated on a glass plate.
The plate is placed on end in a shallow layer of developing solvent mixture in a closed chamber.
The solvent is allowed to rise on the plate to a predetermined distance at which point the plates are removed from the chamber and dried.
www.friendslab.com.cob-web.org:8888 /tlc.html   (0 words)

  
 DETERMINATION OF LIPSTICK DYES BY THIN-LAYER CHROMATOGRAPHY
We will use TLC to separate dyes that are impart to lipstick its color.
Thin layer chromatography utilizes a thin film of silica gel or alumina coated onto a glass or plastic strip.
Prepare a mixture of 5 mL of isoamyl alcohol, 3.5 mL of acetone, 3 of distilled water, and 0.5 mL of ammonium hydroxide to use as a solvent or mobile phase for the chromatography of the lipstick stains.
web.centre.edu /muzyka/che11/131lab/lipstick.htm   (0 words)

  
 : (via CobWeb/3.1 planetlab2.isi.jhu.edu)   (Site not responding. Last check: 2007-10-19)
Thin Layer Chromatography (TLC) is one of the most popular and widely used separation techniques.
When coated on a plate in the form of thin layer it serves as a versatile solid stationary phase for adsorption and partition chromatography.
In the 'reversed' phase Chromatography, silica gel layer is impregnated with non-polar liquids such as silicone oil or hydrocarbons like undecane, paraffin oil etc. Modified silica gel layers can be prepared by incorporating desired chemical reagents such as acids, bases and buffers while preparing slurry of silica gel for coating plates.
www.acmechem.com.cob-web.org:8888 /silica.htm   (0 words)

  
 Thin-layer chromatography - The New Companion   (Site not responding. Last check: 2007-10-19)
By carefully choosing the solvents and by varying the composition of the silica gel layer, chemists have been able to separate a vast range of compounds.
It was Stahl (1924-1986) who put thin-layer chromatography (or TLC, if you want to sound like a pro) in the top drawer of the analytical chemist's tool chest.
By this sort of criterion, thin-layer chromatography must be among the most elegant of all analytical techniques.
www.newcompanion.com /contents/cont03/020825tlc.html   (0 words)

  
 Applied Thin-Layer Chromatography
Thin layer chromatography (TLC) is a powerful, fast and inexpensive analytical method.
Properties and functions of various materials and the TLC equipment are described, covering e.
All this is illustrated with numerous coloured figures.How to use TLC in compliance with GLP/GMP regulations is described in detail, including the required documentation.
www.hsc.wvu.edu /sop/compchem/mdpi/molecules/html/50700983.htm   (0 words)

  
 Thin Layer Chromatography   (Site not responding. Last check: 2007-10-19)
Our glass chromatography tank (pictured with Chrom Clips and compression rod) is ideal for developing paper chromatograms and TLC plates.
Order the chromatography tank as part number 127070 (27 cm x 7.5 cm x 26 cm high).
Order the rods as part number 127074 for tanks 23 to 38 cm wide, 127078 for tanks 14 to 25 cm wide, or part number 127072 for tanks 38 to 66 cm wide.
www.lplc.com /others/tlc.html   (0 words)

  
 Thin Layer Chromatography
TLC is a simple, quick, and inexpensive procedure that gives the chemist a quick answer as to how many components are in a mixture.
A TLC plate is a sheet of glass, metal, or plastic which is coated with a thin layer of a solid adsorbent (usually silica or alumina).
The TLC plate is then placed in a shallow pool of a solvent in a developing chamber so that only the very bottom of the plate is in the liquid.
orgchem.colorado.edu /hndbksupport/TLC/TLC.html   (0 words)

  
 Chromatography, TLC - Thin Layer, HPLC - High Pressure Liquid
High Pressure Liquid Chromatography (HPLC) is a modern adaptation of TLC with improved separation efficiency and detection limits.
HPLC can be used to determine the identity and bulk purity of botanicals and their extracts or compendial drug substances.
The mobile phase is a developing liquid that travels up the stationary phase, carrying the samples with it.
www.ndstechnologiesinc.com /chromatography_products.htm   (0 words)

  
 Thin Layer Chromatography
Thin-layer chromatography (TLC) is a very commonly used technique in synthetic chemistry for identifying compounds, determining their purity and following the progress of a reaction.
As stationary phase, a special finely ground matrix (silica gel, alumina, or similar material) is coated on a glass plate, a metal or a plastic film as a thin layer (~0.25 mm).
The thin end of the spotter is placed in the dilute solution; the solution will rise up in the capillary (capillary forces).
www.chem.ucla.edu /~bacher/General/30BL/tips/TLC1.html   (0 words)

  
 Thin Layer Chromatography, Whatman Thin Layer Chromatography (TLC) Plates, Whatman Blotting and Chromatography Papers, ...
Analtech Cyclograph II TLC System Analtech Cyclograph II TLC System and other Thin Layer Chromatography products are now available to order from the Cole Parmer Instrument Catalog.
Analtech Glass-Backed TLC Uniplates Analtech Glass-Backed TLC Uniplates and other Thin Layer Chromatography products are now available to order from the Cole Parmer Instrument Catalog.
Thin Layer Chromatography (TLC) Accessories Thin Layer Chromatography (TLC) Accessories and other Thin Layer Chromatography products are now available to order from the Cole Parmer Instrument Catalog.
www.coleparmer.com /catalog/Product_index.asp?cls=15305   (0 words)

  
 Analtech - Thin Layer Chromatography
Often TLC plates not specifically described here can be prepared by simple adaptations of Analtech's mass production methods with little or no additional cost.
Analtech will be happy to supply your special TLC plate requirements whenever it is possible.
For quantity discounts on TLC equipment, glassware and instruments, please contact our sales manager, Steven Miles (see contact page).
www.thinlayer.com /order.php?ps_session=46d86f10ecec6c62fb7c31ee95c0077b   (0 words)

  
 Thin Layer Chromatography   (Site not responding. Last check: 2007-10-19)
Thin Layer Chromatography (TLC) is a technique used to separate, identify, and purify compounds, and mixtures of compounds.
A TLC plate is a microscope slide that has had one side coated with Silica Gel.
Then, the plate is lowered into a jar containing a small amount of a solvent.
www.umich.edu /~chem211/Text/TLC.html   (0 words)

  
 Separation of Latent Fingermark Residue by Thin-Layer Chromatography
A method for the separation of the lipid and nitrogeneous constituents of latent fingermarks using thin-layer chromatography (TLC) is described.
Observation of developed plates using ultraviolet fluorescence detection in the region 310–390 nm has determined that the major fluorescent material is lipid in nature and runs with the squalene control band.
After running, the TLC plates were treated with the following latent fingermark enhancement procedures: visible fluorescence, ninhydrin, l,8-diazafluoren-9-one and physical developer.
www.astm.org /JOURNALS/FORENSIC/PAGES/2568.htm   (0 words)

  
 Whatman - Leadership in separations technology for the life sciences
The chain length of the hydrocarbon functional groups primarily affects retention and the ability to accommodate the water content of solvent systems.
Multi-K combines silica gel and reversed phase C18 layers side by side on the same plate.
They can be successfully used for the separation of mixed polarity samples by two-dimensional chromatography utilising two different separation mechanisms.
www.whatman.com /products/?pageID=7.27.20.69   (0 words)

  
 Thin-Layer Chromatography Lab Activity
Simple and quick to perform, our lab activity uses thin-layer chromatography, an analytical tool that separates mixtures of similar molecules based on adsorption, to extract plant chlorophyll.
The chromatography strips separate the molecules of the plant specimens based on their size and shape.
For an additional investigation, students may collect their own plant specimens for chromatography analysis.
www.wardsci.com /product.asp?pn=IG0007463   (0 words)

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