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Chemorepulsion and thymocyte emigration -- Cyster 109 (8): 1011 -- Journal of Clinical Investigation of a role for chemorepulsion in thymocyte emigration, Poznansky Thymocyte emigration is mediated by active movement away from stroma-derived factors. Rapid induction of medullary thymocyte phenotypic maturation and egress inhibition by nanomolar sphingosine 1-phosphate receptor agonist www.jci.org /cgi/content/full/109/8/1011   (1156 words)

Protective effects of anti-C5a in sepsis-induced thymocyte apoptosis -- Guo et al. 106 (10): 1271 -- Journal of ... Twenty-four hours after surgery, thymocytes were harvested from control, sham, CLP-treated with preimmune IgG, and anti-C5 –treated CLP rats, and evaluated for DNA fragmentation and Ax/PI staining using the same protocols described for Figures 1 and 2. Inhibition of caspase activation in thymocytes by in vivo C5a blockade in CLP-induced sepsis. Wang, S.D., Huang, K.J., Lin, Y.S., and Lei, H.Y. Sepsis-induced apoptosis of the thymocytes in mice. www.jci.org /cgi/content/full/106/10/1271   (5703 words)

Mem. Inst. Oswaldo Cruz  vol.99 no.4; Abstract: S0074-02762004000400007   (Site not responding. Last check: 2007-11-04) We found no difference that could explain INS-driven thymocyte growth, in the pattern of transcripts for death/proliferation mediators, or for a series of growth factor receptors and transcriptional regulators known as essential for thymus development. Thymocyte suspensions from cultured lobes, stained for phenotype analysis by fluorescence activated cell sorting, showed a decreased staining for Notch1 protein at cell surfaces upon INS addition. We analyzed the expression of Notch-related elements, and observed the recruitment of a specific set of transcripts simultaneous and compatible with INS-driven thymocyte growth, namely, transcripts for Notch3, for its ligand Jagged2, and for Deltex1, a mediator of a poorly characterized alternative pathway downstream of the Notch receptor. www.scielo.br /scielo.php?script=sci_abstract&pid=S0074-02762004000400007&lng=en&nrm=iso&tlng=en   (245 words)

Dynamics of Thymocyte-Stromal Cell Interactions Visualized by Two-Photon Microscopy -- Bousso et al. 296 (5574): 1876 ... Note that virtually all thymocytes at the surface of the lobe are rounded and non-motile. A thymocyte crawls in from the left, makes an initial contact with a stromal cell in the center, continues crawling, contacts another stromal cell, and returns to the stromal cell in the center. A thymocyte crawls in from the left, stops upon contact with the stromal cell and becomes partially enclosed by the stromal cell. www.sciencemag.org /cgi/content/full/296/5574/1876/DC1   (624 words)

Neuroendocrine Control of Thymus Physiology -- Savino and Dardenne 21 (4): 412 -- Endocrine Reviews thymocytes, with a decrease in the proportion of apoptosis in thymocytes is mediated by transactivation of the thymocyte depletion (193), with an increase in apoptosis (ascertained edrv.endojournals.org /cgi/content/full/21/4/412   (8769 words)

Epidermal Growth Factor (EGF) Modulates Fetal Thymocyte Growth and Differentiation: Partial Reversal by Insulin, ... Thymocytes were obtained from pooled lobes, stained with anti-CD4 and anti-CD8 mAbs, and analyzed by flow cytometry. Thymocytes were obtained from pooled lobes, double-labeled with anti-CD4 and anti-CD8 mAbs, and evaluated by flow cytometry. thymocyte proliferation in the DN DP transition (50, 51). www.jimmunol.org /cgi/content/full/161/7/3384   (6021 words)

Analysis of Thymocyte Development Reveals that the GTPase RhoA Is a Positive Regulator of T Cell Receptor Responses In ... Thymocytes from NLC and V14RhoA mice were isolated, stained with anti-CD4-PE and anti-CD8-FITC, and analyzed by flow cytometry. Freshly isolated thymocytes were attached to fibronectin or poly-L-lysin coated coverslips and analyzed by light microscopy as described previously. CFSE-labeled thymocytes from NLC and V14RhoA transgenic mice were stimulated with soluble anti-CD3 antibodies or PdBu (5 ng/ml) and ionomycin (0.5 ng/ml) as described previously. www.jem.org /cgi/content/full/194/7/903   (5604 words)

Galectin-1, an Endogenous Lectin Produced by Thymic Epithelial Cells, Induces Apoptosis of Human Thymocytes -- Perillo ... and nonselected thymocytes, and that the susceptibility of thymocytes Human thymocytes were isolated from surgical specimens and passaged over nylon wool as previously described (24). Rescue of thymocytes and T cell hybridomas from glucocorticoid-induced apoptosis by stimulation via the T cell receptor/CD3 complex: a possible in vitro model for positive selection of the T cell repertoire. www.jem.org /cgi/content/full/185/10/1851   (5102 words)

Thymocyte Contact or Monoclonal Antibody-Mediated Clustering of alpha 3beta 1 or alpha 6beta 4 Integrins Activate ... Thymocyte supernatant was obtained from thymocytes cocultured with TEC for 12 hours. Thymocyte adhesion to TEC monolayers induces repolarization of TEC and bound thymocytes were detached by trypsin-EDTA, washed, vortexed to disrupt aggregates, and counted by flow cytometry. www.bloodjournal.org /cgi/content/full/92/10/3745   (6435 words)

E-cadherin-mediated interactions of thymic epithelial cells with CD103+ thymocytes lead to enhanced thymocyte cell ... DN thymocytes were found in the run-through of the columns. thymocytes with primary thymic epithelial cells in the absence (A) or presence (B) of anti-CD103 antibodies. SP thymocytes were co-cultured with primary thymic epithelial cells (Ep/CD8+), which induces a net increase in cell proliferation after 48 hours of culture. jcs.biologists.org /cgi/content/full/115/23/4505   (5423 words)

Increased p300 Expression Inhibits Glucocorticoid Receptor-T-Cell Receptor Antagonism but Does Not Affect Thymocyte ... Thymocytes were similarly stimulated, and the IL-2 produced was quantitated 24 h later using the IL-2-dependent cell line HT-2. Thymocytes from four NLC mice (open triangles) and four p300-transgenic mice (solid circles) were untreated (A), activated with different concentrations of dexamethasone (Dex) (B), or immobilized with anti-CD3 (C). The inhibition of GR-TCR antagonism in p300-transgenic thymocytes mcb.asm.org /cgi/content/full/22/13/4556   (4616 words)

Beta-adrenoceptor blockade alters thymocyte differentiation in aged mice.   (Site not responding. Last check: 2007-11-04) To determine if the age-related changes in thymocyte differentiation are modified by NE signaling through beta-adrenergic receptors, 2-month (mo) and 18-mo old BALB/c mice were implanted subcutaneously with pellets containing the non-selective beta-adrenoceptor antagonist nadolol. Four and one-half weeks later, thymus and peripheral blood were collected to assess changes in thymocyte differentiation and naive T-cell output by flow cytometric analysis of T-cell subpopulations. In old mice, but not in young mice, thymocyte CD4/CD8 co-expression was altered by beta-adrenoceptor blockade. www.arclab.org /medlineupdates/abstract_11292254.html   (285 words)

Defective Thymocyte Maturation by Transgenic Expression of a Truncated Form of the T Lymphocyte Adapter Molecule and ... increase in spontaneous thymocyte apoptosis in transgenic mice C, Thymocytes from wild-type and transgenic CR1 mice were cultured with or without dexamethasone for the indicated times and then stained with 7AAD and annexin V. Percent survival is represented as the number of live cells (7AAD and annexin V-negative) remaining at each time interval. A, Thymocytes from 6- to 8-wk-old mice were stained with anti-CD4 and anti-CD8 Abs and analyzed by flow cytometry as in Fig. www.jimmunol.org /cgi/content/full/169/12/6900   (6121 words)

Two Distinct Steps during Thymocyte Maturation from CD4-CD8- to CD4+CD8+ Distinguished in the Early Growth Response ...   (Site not responding. Last check: 2007-11-04) In the earliest phase, thymocytes are characterized by TCR loci Endogenous Egr-1 Expression in DN Thymocytes of Wild-type Mice. IL-7 transgenic mice: analysis of the role of IL-7 in the differentiation of thymocytes in vivo and in vitro. www.jem.org /cgi/content/full/186/6/877   (4672 words)

Dexamethasone-Induced Thymocyte Apoptosis: Apoptotic Signal Involves the Sequential Activation of ... Thymocytes were treated with Dex at indicated doses for 15 minutes. Thymocytes were incubated for 18 hours with 10 µmol/L C2-ceramide in the presence or absence of actinomycin-D (Act-D; 2.5 µg/mL) and cycloheximide (CHX; 50 µg/mL) and analyzed for apoptosis. Decrease of Bcl-xL and augmentation of thymocyte apoptosis in GILZ overexpressing transgenic mice www.bloodjournal.org /cgi/content/full/93/7/2282   (7628 words)

Expression of the p56lck Y505F Mutation in CD45-Deficient Mice Rescues Thymocyte Development -- Seavitt et al. 19 (6): ... Thymocytes were collected, incubated on ice from 2 to 4 h, washed in phosphate-buffered saline (pH 7.4), and lysed in 1 ml thymocytes regardless of the genotype (Table 1 and data not shown). Thymocytes from treated mice were analyzed 20 h postinjection by three-color flow cytometry with annexin V, KJ1-26 (anti-DO11.10 TCR), and propidium iodide. mcb.asm.org /cgi/content/full/19/6/4200   (5302 words)

Constitutive activation of NF-{kappa}B and T-cell leukemia/lymphoma in Notch3 transgenic mice -- Bellavia et al. 19 ...   (Site not responding. Last check: 2007-11-04) thymocytes from wild-type (wt) and tg(+) (tg) mice at different ages (16 and 18 d.p.c., E16 and E18; 0 and 4 post-natal days, 0d and 4d; and indicated weeks). B complexes in nuclear extracts from freshly isolated thymocytes of 3-week-old tg(+) (tg) or wild-type (wt) mice, incubated in the absence (–) (lower arrow) or presence of antibodies against either p50 or p65 or an unrelated antibody against c-myc. Chaffin,K.E., Beals,C.R., Wilkie,T.M., Forbush,K.A., Simon,M.I. and Perlmutter,R.M. (1990) Dissection of thymocyte signaling pathways by in vivo expression of pertussis toxin ADP-ribosyltransferase. embojournal.npgjournals.com /cgi/content/full/19/13/3337   (5810 words)

Profound block in thymocyte development in mice lacking p56lck   (Site not responding. Last check: 2007-11-04) It is expressed exclusively in lymphoid cells, predominantly in thymocytes and peripheral T cells. Lck associates specifically with the cytoplasmic domains of both CD4 and CD8 T-cell surface glycoproteins and interacts with the beta-chain of the interleukin-2 receptor, which implicates Lck activity in signal transduction during thymocyte ontogeny and activation of mature T cells. Mature, single-positive thymocytes are not detectable in these mice and there are only very few peripheral T cells. www.bioscience.org /knockout/ref/molina.htm   (190 words)

Thymocyte development is normal in CTLA-4-deficient mice -- Chambers et al. 94 (17): 9296 -- Proceedings of the ... Cell Division of Thymocyte Subsets Is Unchanged in the Absence of CTLA-4. Thymocytes were loaded with Indo-1 and incubated with anti-CD4-PE and anti-CD8-TC and anti-CD3 (500A2) or 560 Thymocytes from 11-day-old littermate mice were incubated on anti-CD3 antibody or control antibody 560-coated plates for 16-18 www.pnas.org /cgi/content/full/94/17/9296   (4576 words)

Inhibition of Thymopoiesis of CD34+ Cell Maturation by HIV-1 in an In Vitro CD34+ Cell and Thymic Epithelial Organ ...   (Site not responding. Last check: 2007-11-04) HIV infection of thymocytes was predominantly observed in the thymocytes were depleted and thymopoiesis was markedly arrested In vitro studies of HIV-1 expression in thymocytes from infants and children. stemcells.alphamedpress.org /cgi/content/full/17/6/327   (3481 words)

Age-associated thymic atrophy is not associated with a deficiency in the CD44(+)CD25(-)CD3(-)CD4(-)CD8(-) thymocyte ...   (Site not responding. Last check: 2007-11-04) Age-associated thymic atrophy is not associated with a deficiency in the CD44(+)CD25(-)CD3(-)CD4(-)CD8(-) thymocyte population. We have purified these cells from the thymus of both old and young mice and demonstrate no age-associated defect in their ability to differentiate into their progeny in vitro when used to reconstitute fetal thymic organ cultures. We also demonstrate that in the presence of anti-IL-7, CD44(+)CD25(-)CD3(-)CD4(-)CD8(-) cells from young mice show reduced thymocyte development in fetal thymic organ cultures compared with controls. www.arclab.org /medlineupdates/abstract_11748931.html   (177 words)

Kaye Laboratory - Publications Shao, H., Rubin, E., Chen, L.-Y., and Kaye, J. A role for ras signaling in coreceptor regulation during differentiation of a double positive thymocyte cell line J. Immunol. Slow accumulation of active MAP kinase during thymocyte differentiation regulates the temporal pattern of transcription factor gene expression. Han, P., Goularte, O.D., Rufner, K., Wilkinson, B., and Kaye, J. An inhibitory Ig superfamily protein expressed by lymphocytes and APCs is also an early marker of thymocyte positive selection. www.scripps.edu /imm/kaye/pubs.htm   (252 words)

Directory of open access journals   (Site not responding. Last check: 2007-11-04) The function of the thymic microenvironment is to promote thymocyte maturation, in part via regulation of thymocyte proliferation and cell death. Defects in fetal thymic epithelial cell (TEC) development and function, and therefore in the formation of a functional microenvironment, can be caused either directly by TEC differentiation defects or indirectly by defective thymocyte maturation. Wild type fetal and adult thymus showed stage-specific differences in the proliferation profiles of developing thymocytes, with fetal stages showing generally higher levels of proliferation. www.doaj.org /abstract?id=81468&toc=y   (218 words)

LST Molecular Endocrinology Group   (Site not responding. Last check: 2007-11-04) Mann, C.L., Hughes, F.M, and Cidlowski, J.A. (2000) Delineation of the signaling pathways involved in glucocorticoid-induced and spontaneous apoptosis of rat thymocytes. and Cidlowski, J.A. (2000) Delineation of the signaling pathways involved in glucocorticoid induced and spontaneous apoptosis in rat thymocytes. Mann, C.L. and Cidlowski, J.A. (2001) Glucocorticoids Regulate Plasma Membrane Potential During Rat Thymocyte Apoptosis in vivo and in vitro. dir.niehs.nih.gov /dirlst/groups/cidlowski.htm   (908 words)

Anti- Lymphocyte (ALS) and Anti- Thymocyte (ATS) antibodies from Research Diagnostics Inc Anti-Mouse Thymocyte, and anti-Rat thymocyte antisera are made using RBC-free mouse and rat thymocytes respectively. Adsorbed anti-mouse thymocyte and adsorbed anti-rat thymocyte antisera are anti-thymocyte absorbed with mouse spleen cells and rat spleen cells respectively. These absorbed antisera generally show >10 fold cytoxic antibody titers for thymocyts than for spleen cells. www.researchd.com /miscabs/lymphab.htm   (640 words)

Cancer Research Laboratory 2003-2004 Bousso, P and Robey, E (2004) Visualizing Thymocyte Motility Using 2-Photon Microscopy. thymocytes responding to T cell receptor, Notch and positive selection signals. Immunoglobulin Heavy Chain V to DJ Rearrangement in Transgenic Thymocytes. www.biol.berkeley.edu /crl/crl_annual_report.html   (4226 words)

Medullar Thymocyte Structure and Density in the Chicken after Experimental Gamma Irradiation   (Site not responding. Last check: 2007-11-04) Medullar Thymocyte Structure and Density in the Chicken after Experimental Gamma Irradiation. The aim of this study was to quantify the chicken thymic medullar lymphocytes after a whole-body irradiation, and to study changes in thymus cell morphology. For problems or questions regarding this web contact actavet@vfu.cz www.vfu.cz /acta-vet/vol69/139-00.htm   (236 words)

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