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Topic: Tissue culture

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	Tissue Culture (Site not responding. Last check: 2007-10-22)
		Tissue culture consists of growing plants cells as relatively on organized masses of cells on an agar medium (callus culture) or as a suspension of free cells and small cell masses in a liquid medium (suspension culture).
		Tissue culture is used for vegetative multiplication of many species and in some cases for recovery of virus-free plants.
		Tissue culture is the process whereby small pieces of living tissue (explants) are isolated from an organism and grown aseptically for indefinite periods on a nutrient medium.
	www.indiaagronet.com /indiaagronet/seeds/CONTENTS/tissue_culture.htm (1623 words)

	Plant Tissue Culture (Site not responding. Last check: 2007-10-22)
		Plant tissue culture involves the growing of plant tissue from plant material taken from a source plant.It has been found that plants can reproduce whole plants from fragments of plant material when given a nutrient media capable of supporting growth and appropriate hormone control.
		Cell division in tissue had to await the discovery of kinetin.This was first discovered by autoclaving freshly isolated slurries of DNA from Herring sperm.The discovery of cytokinins gave much impetus to tissue culture.
		Tissue culture is thus proving to be useful in a variety of ways including plant propagation,raising and maintenace of high health status plants,germ plasm storage,and a valuable technique in plant improvement..In plant improvement tissue culture maybe used in the technique of gene insertion to improve plant stocks.
	members.aol.com /MrDJReed/private/PTC.html (252 words)

	Tissue Culture Explained
		Tissue culture was first successful in the 70's mainly to protect endangered and rare plants from extinction.
		Another great thing about tissue culture is that if there is a popular hybrid species from cross pollination human efforts or plants pollinated by accident that refuses to produce viable seeds, the tissue culturing is that perfect way around the hybrid end to that new species.
		Most of the time the consumer has nothing to worry about with a tissue cultured plant since they are sold at the safe stage of the plant's life to nurseries and the nurserymen are left to raise them past the crucial stage of their lives.
	www.centralfloridafarms.com /tculture.htm (1093 words)

	Tissue Culture?
		Tissue culture is when a piece of plant is put into the tiniest of greenhouses...a test tube....and a miracle takes place.
		Tissue cultured plants are micro propagated cuttings or clones, genetically identical to the mother and all the daughter plants.
		Tissue culture is used for many reasons but the 3 major reasons DnA Gardens are concerned with are 1) Large quantities of plant production for the sake of volume, 2) to speed up the production of new varieties into the market place.
	www.dnagardens.com /tissue.htm (522 words)

	Beginning Molecular Biology Laboratory Manual
		Tissue culture is often a generic term that refers to both organ culture and cell culture and the terms are often used interchangeably.
		Cultures should be examined daily, observing the morphology, the color of the medium and the density of the cells.
		Prepare a cell suspension, either directly from a cell culture or from a concentrated or diluted suspension (depending on the cell density) and combine 20 μl of cells with 20 μl of trypan blue suspension (0.4%).
	www.research.umbc.edu /~jwolf/method5.htm (2909 words)

	The Many Dimensions of Plant Tissue Culture Research
		The major impact of plant tissue culture will not be felt in the area of micropropagation, however, but in the area of controlled manipulations of plants at the cellular level in ways which have not been possible prior to the introduction of tissue culture techniques.
		Plant tissues known to be free of the pathogen under consideration (viral, bacterial, or fungal) are physically selected as the explant for tissue culture.
		Cultures which reveal the presence of the pathogen are destroyed, while those which are indexed free of pathogen are maintained as a stock of pathogen-free material.
	aggie-horticulture.tamu.edu /tisscult/pltissue/pltissue.html (1693 words)

	Tissue culture project
		The purpose of this study was to determine which tissue culture technique (root, leaf, stem) produces the tallest plantlet in a given time.
		Tissue culture is a way to vegetative propagate plants using small cuttings to produce more than one plant; some times thousands.
		The best part about tissue culture is that if you use multiplication media the plants will keep multiplying until you stop them and then transfer them into pretransplant media, which stops multiplication and starts root development.
	stone.web.brevard.k12.fl.us /science/research/student/jordona1 (1034 words)

	Cell Biology Lab- Tissue Culture Methods (Site not responding. Last check: 2007-10-22)
		Primary cell cultures are derived from rat, mouse and human in the Lab.
		Cultures can be generated from embryonic or adult: cortex, retina, spinal cord, dorsal root ganglia, sympathetic ganglia.
		The disadvantages of primary cultures are the mixed nature of each preparation, limited lifespan of the culture and the potential contamination problems.
	anatomy.med.unsw.edu.au /cbl/research/methods/culture.htm (232 words)

	Tissue Culture micropropagation : Basics (Site not responding. Last check: 2007-10-22)
		Plant tissue culture (PTC) is a generic description which embraces plant protoplast, plant cell, plant tissue, plant organ and plant culture, where these various types of culture involve, as a common factor, the growth of microbe-free plant material in an aseptic (sterile) environment, such as sterilized nutrient medium in a test tube.
		Callus cultures and suspension cultures, in contrast, begin with pieces of tissues and cells from an organized part of the plant but cultural conditions are manipulated to create an unorganized state, e.g.
		Much of this research is done in culture vessels, such as test tubes, which are not economical in commercial operations, and we now know that such matters as size and shape of culture vessel, how they are "closed" and the volume of medium in them, can have a marked effect on the growth of cultures.
	free-blog-site.com /tissue_culture/archive/category/1508.aspx (5976 words)

	Tissue culture - Wikipedia, the free encyclopedia
		A tissue culture is the growth of cells (tissue) separate from the organism.
		In modern usage, "tissue culture" often refers to the growth of vertabrate or plant cells in vitro.
		In this sense, tissue culture, along with recombinant DNA is a cornerstone of biotechnology.
	en.wikipedia.org /wiki/Tissue_culture (238 words)

	Tissue Culture
		The main objective of the Tissue Culture Unit is to strengthen the propagation capability of the Ministry of Agriculture and the wider Agricultural Sector through production of disease-free plant material via micro-propagation techniques and germplasm conservation of selected plant species through in vitro techniques.
		Tissue culture is utilized in the field of Biotechnology.
		The composition of the cultural media is one of the most important factors in determining the growth and morphology of in vitro plants.
	www.slumaffe.org /Agriculture/Crop_Development/Tissue_Culture/tissue_culture.html (559 words)

	Cell Biology Lab- Visit the Tissue Culture Lab
		Tissue Culture is where cells are grown either as isolated cells or small pieces of tissue under conditions which copy those they would normally be exposed to in the animal or human.
		To do Tissue culture we need a sterile work area, sterile solutions and stocks, the cells, an incubator to grow them, and finally a way of looking at the cells.
		Sterile solutions and tissue culture plates coated with adhesive molecules are stored in the fridge at 4C or in the freezer -20C.
	anatomy.med.unsw.edu.au /cbl/lab/visit_tc.htm (566 words)

	Kitchen Culture Kits - Tissue Culture Propagation of Aquatic Plants
		Plantlets, whether rooted in tissue culture, or in soil, must then be acclimated to the outside world (lower humidity, higher temperatures, diseases and pests).
		Tissue culture techniques can also be used on seeds that are hard store, or are difficult to germinate in nature.
		Plant tissue culture can be done in the kitchen, using a microwave oven to sterilize media, PPM, a biocide, to minimize contamination, and a simple box to limit dust falling into culture vessels (see: www.kitchenculturekit.com).
	www.kitchenculturekit.com /aquatic.htm (2621 words)

	Plant tissue culture - Wikipedia, the free encyclopedia
		Plant tissue culture, also called micropropagation, is a practice used to propagate plants under sterile conditions, often to produce clones of a plant.
		Plant tissue culture relies on the fact that many plant cells have the ability to regenerate a whole plant (totipotency).
		As plant tissue culture is a very labour intensive process, not all plants are suitable to be commercially propagated in a laboratory.
	en.wikipedia.org /wiki/Plant_tissue_culture (843 words)

	Plant Tissue Culture
		Plant Tissue Culture, more technically known as micropropagation, can be broadly defined as a collection of methods used to grow large numbers of plant cells, in vitro, in an aseptic and closely controlled environment.
		The medium used to grow the plant tissue, the plant tissues themselves, and the environment surrounding the tissue culture, must be free of all possible contaminants.
		Plant tissue culture is also advantageous to growers because the overwhelming number of plants can be produced using the tissue collected from a single parent plant – a plant which itself remains unharmed in the tissue harvesting process.
	www.bio.davidson.edu /people/kabernd/seminar/2002/method/amy/aj.htm (648 words)

	Tissue Culture - Outstanding Quality - Leading Innovation
		Tissue culture, simply put, is the method of keeping tissue alive and growing in vitro in a tissue culture media containing a rich mixture of essential amino acids, vitamins, and peptides, thus replicating conditions in which the tissue normally grows in an animal or human.
		Applications and uses of tissue culture in the biosciences are myriad, and growing exponentially.
		From quantitative tissue culture, the "gold standard" in microbiology and medical diagnosis, to the development and growth of new tissue culture cell lines, standard research methods are being optimized and adapted for new applications, and new techniques are being developed and tested.
	www.invitrogen.com /tissue_culture.htm (888 words)

	TPP Tissue Cell Culture Products for the Laboratory
		Our new range of tissue culture flasks, available with filtered or vented caps, is the result of intensive cooperation between user experts and top development specialists.
		These optically clear, high-grade polystyrene dishes are tissue culture treated only on the base of the dish with a non-treated ring surrounding the growing surface to prevent cell attachment on the edge.
		The new generation of Tissue Culture Plates from TPP is characterized by yellow strip marking areas and easy-to-read fl-embossed, alpha-numeric writing.
	www.tissue-cell-culture.com /docs/TC/tissue-cell-culture-products.html (1045 words)

	Tissue Culture & Somatic Embryogenesis
		Tissue culture propagation development and its application to energy crops.
		Somatic embryogenesis and plant regeneration in tissue cultures of sweet potato (Ipomoea batatas Poir.).
		Composition of embryogenic suspension cultures of Ipomoea batatas Poir.
	www.hos.ufl.edu /djcweb/Pub2.htm (771 words)

	Tissue Culture Trouble-Shootin
		Insect cell culture media, as well as a few mammalian media such as L-15, contain buffering systems that are not dependent on CO These cultures should be exposed to atmospheric conditions.
		One initial method to confirm suspicion of contamination is to transfer fluids from the suspected culture to a "clean" culture, incubate, and observe daily for cell deterioration.
		Contaminated cultures should be autoclaved and discarded immediately to reduce the risk of spreading the contamination to other cultures or other areas of the lab.
	www.tissue-cell-culture.com /docs/libary/tc_trouble.html (2986 words)

	Plant Tissue Culture
		Plant tissue culture (micropropagation) is a technique which will do just that for us.
		As for being technical, you can begin plant tissue culture with as little as a cookbook approach and a feeling for sterile technique.
		Other decisions will be based upon why you are doing plant tissue culture and what you expect as a result (more plants?).
	www.accessexcellence.org /LC/ST/st2bgplant.html (648 words)

	Ornamental Crops: tissue culture
		A study of light and temperature levels is seeking to determine the optimum storage conditions and acclimatization techniques for tissue culture propagation.
		The study is using tissue culture samples, called micropropagules, of several Hosta varieties to test their response to varying levels of light and temperature, as well as liquid sucrose, during storage.
		Another tissue culture study is being conducted using medicinal plants in a system that was developed and patented by Clemson scientists in 1998.
	www.clemson.edu /scg/orn/tissue.htm (197 words)

	Tissue Culture (Site not responding. Last check: 2007-10-22)
		While the PET membranes have individual, cylindrical pores, which are tissue culture treated to facilitate cell-growth, the side walls are left untreated to limit the growth.
		They are used mainly for studying anchorage-dependent and anchorage-independent cells, for epithelial and endothelial cell culture and microbial pathogenesis studies.
		These too are tissue culture treated for better cell attachment and growth and have windows on two sides for viewing purposes.
	www.asi-supplies.com /TissueCulture.htm (900 words)

	Tissue Culture Facility and Media Center - UMass Medical School
		Tissue culture media can either be conveniently ordered for next day delivery or picked-up for immediate use (commonly used media products only).
		The Tissue Culture Facility is located on the 1st floor of Biotech I in Room 141.
		Tissue culture supplies should be ordered by either FAX (508-856-3190) or email (tissueculture@umassmed.edu).
	www.umassmed.edu /tissueculture (350 words)

	Succulent Tissue Culture
		On this page STC will inform you on all related subjects to the tissue culture and micropropagation of succulents, caudiciform plants and other interesting subjects.
		In vitro culture as a potential method for the conservation of endangered plants possessing crassulacean acid metabolism
		Regeneration from Phylloclade explants and callus cultures of Schlumbergera and Rhipsalidopsis
	www.succulent-tissue-culture.com /other.htm (457 words)

	MedlinePlus Medical Encyclopedia: Culture - gastric tissue biopsy
		A gastric tissue biopsy is an examination of a sample of gastric (stomach) tissue for culture (checking for the presence of infection-causing organisms). The test is done to look for the presence of certain microorganisms that may be playing a role in certain diseases.
		An endoscopy may be performed to determine the presence of ulcers, for which pathology and culture specimens are often useful.
		Certain infections may also be diagnosed by endoscopy; therefore, a culture of tissue obtained by endoscopy may be done.
	www.nlm.nih.gov /medlineplus/ency/article/003728.htm (628 words)

	Company Information about Winterberry Farms Tissue Culture (Site not responding. Last check: 2007-10-22)
		Winterberry Farms Tissue Culture was established in 1990 to grow plants for the parent company, Rowen Gardens (established in 1988).
		In 1994, the tissue culture laboratory started selling plants to other growers and began to specialize in the propagation of Hosta.
		He is available to do consulting on plant propagation, plant tissue culture and give lectures, or more accurately talks to lay audiences, on several aspects of gardening.
	rowinter.com /WF/HTM_files/AboutWFTC.php (524 words)

	Tissue Culture Facility, UNC Lineberger Comprehensive Cancer Center, cell culture and molecular biology research ... (Site not responding. Last check: 2007-10-22)
		Tissue Culture Facility, UNC Lineberger Comprehensive Cancer Center, cell culture and molecular biology research services and products
		The tcf is a UNC Lineberger Comprehensive Cancer Center core facility dedicated to the support of in vitro cell culture and molecular biology research.
		In addition to information pages and links, the tcf's cell repository information, cell culture prices, and molecular biology product prices are available for viewing and are fully searchable databases.
	www.unc.edu /depts/tcf/info.html (146 words)

	Amazon.com: The Neuron in Tissue Culture: L. W. Haynes (Site not responding. Last check: 2007-10-22)
		Three further tutorial chapters review artefacts of the tissue culture environment, the understanding and control of neuronal apoptosis in vitro, and the culture substratum.
		The volume concludes with five chapters containing short protocols describing systematically the maintenance and propagation of neurons from mammalian and avian peripheral and central nervous systems, stem cell populations, invertebrate neurons, and finally selected organised and reconstruction techniques which are given prominent treatment throughout the book.
		The Neuron in Tissue Culture is a comprehensive reference source dealing exclusively with the tissue culture of neurons.
	www.amazon.com /Neuron-Tissue-Culture-W-Haynes/dp/0471975052 (608 words)

	ATCC: Tissue Culture and Hybridoma Development Products
		DMSO is used as a cryoprotectant in the freezing of cell cultures.
		Feeder layer cell populations are used to support the growth of a variety of fastidious cultured cell types including stem cells.
		In culture, the cells serve as a basal layer for other cells and supply important metabolites without further growth or division of their own.
	www.atcc.org /Products/TissueCulture.cfm (194 words)

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