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| | HRG Tokushima: Molecular and Cellular Characterization of Histidine-Rich Glycoprotein (HRG) Deficiency -- Shigekiyo et ... |
 | | From the First Department of Internal Medicine, School of Medicine, The University of Tokushima, Tokushima; Department of Life Science, Faculty of Science, Himeji Institute of Technology, Hyogo, Japan; and Department of Biochemistry, University of Washington, Seattle, WA. |
 | | To elucidate if the mutation in HRG Tokushima is responsible for the secretion defect of the mutant protein in the proband, we performed pulse-chase experiments of Tokushima-type HRG using transiently transfected BHK cells, and compared the results with those obtained for wild-type HRG. |
 | | Both wild-type and Tokushima-type HRG were detected as a band of 62 kD (Fig 3, left arrowhead) and 72 kD (right arrowhead) in the cell extracts and culture medium, respectively, and they were synthesized at nearly equal levels in BHK cells as detected in cell extracts at time 0 of the chase. |
| www.bloodjournal.org /cgi/content/full/91/1/128 (4093 words) |
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