| | Subcellular localization of host and viral proteins associated with tobamovirus RNA replication (Site not responding. Last check: 2007-10-17) |
 | | The simultaneous appearance of NtTOM1, NtTOM2A and the tonoplast marker V-PPase in fractions 4 and 5 in 'Infect 2' (Figure 5C) is in good agreement with the assumption that the TOM proteins are tonoplast proteins. |
 | | Since most tonoplast proteins are synthesized on the ER membranes and transported to the tonoplasts via the Golgi stacks and other compartments (Jiang and Rogers, 1998; 1999), TOM1 found in Fraction M may represent the protein in such intermediate compartments. |
 | | In addition, since most of the previous studies were performed using cells at later stages of tobamovirus infection, it is possible that the excess 130K/180K proteins produced in the infected cells obstructed the detection of the tonoplast-bound replication proteins. |
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