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Topic: Transfection

	Altogen Biosystems - Cell Transfection Technology Company
		Altogen Biosystems develops polymer-, lipid-, and dendrimer-based transfection technology by applying expertise in molecular biology, combinatorial chemistry, and cell biology.
		Transfection is the transient or stable introduction of exogenous molecules and genetic material, RNA or DNA, into cultured cells and is commonly utilized in biological laboratories for studying gene function, modulation of gene expression, biochemical mapping, mutational analysis, and protein production.
		Unfortunately, no single delivery method or transfection reagent can be applied to all types of cells; cellular cytotoxicity and transfection efficiencies vary dramatically depending on the reagent, protocol and cell type being utilized.
	www.altogen.com (367 words)

	Altogen Biosystems - Transfection Resource
		Transfection is the transient or stable introduction of exogenous molecules and genetic material, DNA or RNA, into cultured mammalian cells and is commonly utilized in biological laboratories for studying gene function, modulation of gene expression, biochemical mapping, mutational analysis, and protein production.
		Transfection by calcium phosphate was one of the earliest chemical based methods developed to deliver exogenous nucleic acids to cultured mammalian cells.
		The transfection reaction is usually performed under aqueous conditions which enables the lipophilic portion of the amphiphilic compound to form the micelle core within which the exogenous nucleic acids are ensconced.
	www.altogen.com /transfection.html (2301 words)

	Stable Transfection
		Stable transfection is the type of transfection where genetic material that is introduced into a cell is retained beyond reproduction.
		Transfection is the process in which foreign DNA is introduced into eukaryotic cells through chemically-created "gates" in the cell membrane.
		In some transfections, DNA enters the cell nucleus and is added to or completely replaces the DNA of the eukaryotic cell.
	www.iscid.org /encyclopedia/Stable_Transfection (245 words)

	Anionic Liposomal Delivery System for DNA Transfection (Site not responding. Last check: 2007-10-28)
		Transfection efficiency of anionic lipoplexes was similar to that of cationic liposomes (lipofectAmine), whereas their toxicity was significantly lower.
		For transfection experiments conducted in the presence of serum, the cells were incubated and transfected in original complete growth medium, omitting rinsing of the cells with HBSS.
		Transfections were performed under identical conditions to determine the transfection efficiencies of anionic lipoplexes, their components, and lipofectAmine, using untreated cells as controls.
	www.aapspharmsci.org /view.asp?art=aapsj060429 (4140 words)

	Transfection
		Transfection can be used for the study of gene function or to treat diseases caused by mutant genes.
		Transfection can be used to treat a disease if genes that fail to produce the needed proteins cause the disease.
		Transfection is useful in the study of the causes of disease and also in possible treatments and cures for genetic diseases.
	www.samford.edu /~gekeller/robinson.html (655 words)

	BioMed Central \| Full text \| Characterization of cationic lipid DNA transfection complexes differing in susceptability ...
		Transfection efficiency could be further increased for all ratios of DOTAP and DC to DNA by addition of the DNA condensing agent protamine sulfate (PS).
		Amongst the barriers for transfection, serum has been reported to exhibit its inhibitory effect either by diminishing the amount of lipid DNA complexes associating with the target cells [3] or via serum proteins binding to lipid DNA complexes thereby diminishing their ability to deliver the transgene [28].
		The functionality of the complexes were analyzed by transfection of 293A target cells and subsequent measurement of the luciferase activity in the cell extracts of the transfected cells.
	www.biomedcentral.com /1472-6750/2/12 (5636 words)

	Transfection
		Transfection is a method by which experimental DNA may be put into a cultured mammalian cell.
		Such experiments are usually performed using cloned DNA containing coding sequences and control regions (promoters, etc) in order to test whether the DNA will be expressed.
		Since the cloned DNA may have been extensively modified (for example, protein binding sites on the promoter may have been altered or removed), transfection is often used to test whether a particular modification affects the function of a gene.
	www.iscid.org /encyclopedia/Transfection (95 words)

	Transfection Reagents for siRNA & Stable Transfection - Invitrogen
		Stable transfection, as opposed to transient transfection, refers to transfection in which genetic material is either added to or completely replaces the nucleus of another cell.
		With stable integration, the transfected DNA becomes a permanent part of the genetic makeup of the cell, and as such, is retained through and beyond reproduction.
		Lipofectin® Transfection Reagent: a trusted reagent for transient and stable transfection of endothelial cells.
	www.invitrogen.com /transfection.htm (604 words)

	TransPass HeLa Transfection Reagent, Transfection Reagents, New England Biolabs
		The transfection complex mixture is composed of 3 µg plasmid DNA expressing GFP and 9 µl TransPass HeLa Transfection Reagent in 1 ml of serum-free DMEM/high glucose.
		Transfected cells were observed microscopically at 20X magnification by either phase (left) or fluorescence (right) microscopy.
		The introduction of cationic lipids or polymers as transfection agents has led to the development of reagents that efficiently deliver DNA through the cell membrane and into the nucleus (3).
	www.neb.com /nebecomm/products/productM2556.asp (623 words)

	Support: Transfection Reagents
		High transfection efficiency depends also on both the promoter under which the gene of interest is expressed and the cell line used.
		The transfected gene expression can be monitored 24-48 hours (transient expression) or 10-15 days (stable expression) after the transfection.
		ExGen 500 high transfection efficiency is not affected in the presence of serum (2).
	www.fermentas.com /techinfo/transfection/considerations.htm (1784 words)

	Transfection Kits
		Transfected cells integrate and express the exogenous DNA at a higher efficiency, producing stable clones of the specifically altered genotype and phenotype.
		The DEAE Dextran mediated transfection method is used for highly efficient transient expression of cloned genes.
		Transfection of these other cell types requires modification of the protocol with respect to the time of incubation of cells with DEAE dextran-DNA-media and chloroquine.
	www.specialtymedia.com /01Products/0602_TransfectionKits.htm (202 words)

	Catalog, Transfection Reagents: ExGen 500 in vitro Transfection Reagent
		ExGen 500 is suitable for the transfection of a wide number of cell types (see the list) with high efficiency and minimal toxicity.
		Transfection can be performed in the presence or absence of serum.
		ExGen 500 transfection efficiency is not affected by the presence of serum (4, 5).
	www.fermentas.com /catalog/transfection/exgeninvitro.htm (389 words)

	Active Motif » Chariot Protein Transfection
		These methods are useful but are very time-consuming as once transfection is completed, it takes 12-80 hours to detect expression of the gene of interest.
		Chariot is a novel transfection method that eliminates many of the problems encountered with more commonly used procedures.
		This, coupled with the fact that Chariot is non-cytotoxic, ensures that observed changes are the result of your recombinant protein, and not the transfection method.
	www.activemotif.com /catalog/cell_biology/chariot (812 words)

	RiboJuice™ siRNA Transfection Reagent
		When transfected into the cell, the siRNA directs cleavage of the target message resulting in suppression of protein expression without affecting non-target genes.
		The other mixture contained 3 µl RiboJuice™ siRNA Transfection Reagent and either 0.1, 0.25, 0.5, 1, 2.5, 5, 10, or 25 nM (final concentration) of the indicated siRNAs.
		Cells were lysed with Reportasol™ Extraction Buffer 24 h after transfection and assayed for reporter activity.
	www.emdbiosciences.com /html/NVG/transfection03.html (318 words)

	SAINT transfection technology FAQ
		There is an enormous difference in 'transfectability' of cells, for example non-dividing and non-adherent cells are difficult to transfect, while other cell types are easy to transfect.
		Transfection with SAINT is not inhibited in the presence of
		The transfection kinetics are not completely clear and some cell types, maybe due to extra divisions, need more time to get expression of the protein than others.
	www.synvolux.com /faq.htm (1100 words)

	Transfection Contents
		In "The Transfection", Megan Omera, a young woman molecular biologist, who works for Omnigene, a biotech company in Monterey, CA, discovers that the DNA she has been engineering into retroviral vectors is not what it is supposed to be.
		"The Transfection" is a chilling novel about the outer boundaries of cloning and genetic engineering, that will be enjoyed and appreciated not only by science fiction fanatics, but also the average reader.
		If The Transfection isn't already happening, it is only a matter of time...
	tinaancinec.com /transfection/transfection.htm (509 words)

	Transfection Optimization :: moleculA
		Poorly maintained cell cultures will have lower transfection efficiency. Developing a growth curve and proper seeding density for routinely subculturing cells is needed to ensure healthy cultures are maintained. Since cells may gradually change in culture using cells within a defined passage number will minimize variability in experiments.
		An important factor for obtaining high transfection efficiency is cell density at the time of transfection. For most adherent cells, cell growth should cover 50-70% of the total plating area before performing a transfection. In general, it is easier to control the level of growth by using larger well plates.
		An additional factor to consider when optimizing transfection is the duration that cells are exposed to the siRNA/liposome complex. As a general rule, the longer the exposure time, the higher the efficiency rate.
	www.molecula.com /new/transfection_optimization.html (504 words)

	Promega - Transfection
		CodeBreaker™ siRNA Transfection Reagent facilitates efficient siRNA transfer into mammalian cells, allowing siRNA-mediated gene silencing with minimal levels of cell death compared to other siRNA transfection reagents.
		The Tfx™ Reagents may be used to transfect cells in the presence of serum.
		The Transfection Assistant is a database of conditions used successfully for transfection of various cell lines using Promega's transfection reagents.
	www.promega.com /applications/transfxn (281 words)

	Opening The Door To Transfection - Biocompare Technology Spotlight
		Now, Novagen is pleased to offer three additions to their transfection reagent or "juice" family: Insect GeneJuice Transfection Reagent (for insect cell transfections), RiboJuice siRNA Transfection Reagent, and ProteoJuice Protein Transfection Reagent.
		FuGENE 6 Transfection Reagent is an advanced non-liposomal formulation that transfects a very wide variety of eukaryotic cells with high efficiency and minimal cytotoxicity.
		Transfection of eukaryotic cell types, including primary cultures and "hard-to-transfect" cell lines, with DNA, including very large plasmids (10 kb), oligonucleotides, or nucleotides.
	www.biocompare.com /techspotlight/180/Opening-The-Door-To-Transfection.html (788 words)

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