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Topic: Transposon

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	Transposon - Wikipedia, the free encyclopedia
		Transposons are sequences of DNA that can move around to different positions within the genome of a single cell, a process called transposition.
		Both classes of transposon may lose their ability to synthesise reverse transcriptase or transposase through mutation, yet continue to jump through the genome because other transposons are still producing the necessary enzyme.
		While transposons may confer some benefits on their hosts, they are generally considered to be selfish DNA parasites that live within the genome of cellular organisms.
	en.wikipedia.org /wiki/Transposon (1247 words)

	Transposons
		Transposons are segments of DNA that can move around to different positions in the genome of a single cell.
		After the transposon is ligated to the host DNA, the gaps are filled in by Watson-Crick base pairing.
		Often transposons lose their gene for transposase; but as long as somewhere in the cell there is a transposon that can synthesize the enzyme, their inverted repeats are recognized and they, too, can be moved to a new location.
	users.rcn.com /jkimball.ma.ultranet/BiologyPages/T/Transposons.html (2081 words)

	Advantages of transposon tagging in Arabidopsis (Site not responding. Last check: 2007-10-06)
		Revertants arise by excision of the transposon from the mutant gene.
		The efficiency of transposon tagging has been improved by using transposons that 1) are immobile and maintained in separate lines from the transposase, 2) carry selectable markers, and 3) disrupt a selectable marker gene whose function is restored upon excision, providing a means of identifying plants in which transposition has occurred.
		Recent transposons carry reporter genes which are activated upon insertion into a gene or promoter, facilitating analysis of the gene's expression pattern.
	www.lsc.psu.edu /ptl/advaarab.html (397 words)

	Xis Protein Binding to the Left Arm Stimulates Excision of Conjugative Transposon Tn916 -- Connolly et al. 184 (8): ...
		Excision of the conjugative transposon Tn916 in Lactococcus lactis.
		Characterization of the Tn916-like transposon Tn3872 in a strain of Abiotrophia defectiva (Streptococcus defectivus) causing sequential episodes of endocarditis in a child.
		Excision of a conjugative transposon in vitro by the Int and Xis proteins of Tn916.
	jb.asm.org /cgi/content/full/184/8/2088 (5531 words)

	[No title] (Site not responding. Last check: 2007-10-06)
		The term"selectable marker"refers to a gene that, when carried by a transposon, alters the ability of a cell harboring the transposon to grow or survive in a given growth enviroment relative to a similar cell lacking the selectable marker.
		For example, the selectable marker gene within the transposon of the invention may influence the protein expression when a construct obtained by the method of the invention is inserted into a protein expression plasmid.
		The transposon nucleic acid according to claim 1 or 2, wherein said transposon end sequence is Mu or Tn7 end sequence.
	www.wipo.int /cgi-pct/guest/getbykey5?KEY=03/87370.031023&ELEMENT_SET=DECL (4864 words)

	Transposon Database Introduction
		The database consists of sequences adjacent to cloned germline transposon insertions in mouse.
		The transposons have different attributes that may be important for future use of the transposon.
		At this time, most of the transposon insertions are on the FVB/n strain background and were created by mobilization of a GFP poly(A)-trap transposon mobilized from a multi-copy concatomer on proximal mouse chromosome 9 (Dupuy et al., 2001).
	mouse.ccgb.umn.edu /transposon/pages/database.html (363 words)

	Transposon Mutagenesis of the Mouse Germline -- Carlson et al. 165 (1): 243 -- Genetics
		Transposon Mutagenesis of the Mouse Germline -- Carlson et al.
		to a splice acceptor in the transposon vector.
		Likewise, splicing of the splice donor within the transposon with the downstream endogenous exon was examined using the same assay with appropriate primers.
	www.genetics.org /cgi/content/full/165/1/243 (6091 words)

	Transposon pools (Site not responding. Last check: 2007-10-06)
		It is often useful to isolate a collection of transposon insertions at many different sites in the genome of a bacterium.
		A population of cells that each contains transposon insertions at random positions in the genome (a transposon "pool") can be used to select or screen for those cells with insertions in or near a particular gene.
		To isolate insertions in or near a gene, cells with transposon insertions are first selected by plating onto rich medium containing an antibiotic resistance expressed by the transposon.
	www.sci.sdsu.edu /~smaloy/MicrobialGenetics/topics/transposons/Tn-pools.html (233 words)

	A Direct Transposon Insertion Tool for Modification and Functional Analysis of Viral Genomes -- Vilen et al. 77 (1): ...
		In vitro DNA transposition reaction with custom-designed mini-Mu transposon as a donor DNA and bacteriophage PRD1 native genome as a target DNA generates recombinant PRD1 viruses as indicated.
		Linear mini-Mu transposons used in this study are defined as segments of DNA that contain 50 bp of Mu R-end DNA as inverted repeats at each end (31, 56).
		Transposon integration sites in phage clones that generated blue and colorless plaques in the blue-white screen are indicated above and below the depicted genome, respectively.
	jvi.asm.org /cgi/content/full/77/1/123 (5704 words)

	Murine Cytomegalovirus with a Transposon Insertional Mutation at Open Reading Frame m155 Is Deficient in Growth and ...
		The stability of the genome and transposon insertion of Rvm155 during viral replication in SCID mice.
		The stability of the transposon insertional mutation of Rvm155 in CB17 SCID mice.
		Construction and transposon mutagenesis in Escherichia coli of a full-length infectious clone of pseudorabies virus, an alphaherpesvirus.
	jvi.asm.org /cgi/content/full/78/13/6891 (6487 words)

	Enhancer and Gene Trap Transposon Mutagenesis in Arabidopsis (Site not responding. Last check: 2007-10-06)
		DsG is the Gene trap transposon, DsE is the enhancer trap transposon.
		However, spontaneous mutants arise at a surprisingly high frequency in transposon lines, and it is important to determine whether the transposon is responsible for any observed phenotype.
		The parental transposon is not selected against by this procedure, and so a large proportion (more than half) of the resulting plants will still have the transposon inserted at the original location.
	genetrap.cshl.org /traps.html (5852 words)

	Promiscuous Target Interactions in the mariner Transposon Himar1 -- Lipkow et al. 279 (47): 48569 -- Journal of ...
		The DNA fragments encoding the transposon ends are illustrated as a single thick line with the end of the element represented by an arrowhead.
		A titration of transposase concentration in the presence of absence of 10% PEG 8000 is shown.
		Transposase and the transposon end fragment were first mixed together and incubated at 30 °C to allow PEC formation.
	www.jbc.org /cgi/content/full/279/47/48569 (5504 words)

	A Transposon-Based Strategy for Sequencing Repetitive DNA in Eukaryotic Genomes -- Devine et al. 7 (5): 551 -- Genome ...
		A plasmid is identified with an insert spanning the cosmid gap, a transposon library
		The IR elements closely resemble a number of DNA transposons that are known to transpose by cut and paste mechanisms.
		Tn5supF, a 264-base-pair transposon derived from Tn5 for insertional mutagenesis and sequencing DNAs cloned in phage lambda.
	www.genome.org /cgi/content/full/7/5/551 (5556 words)

	Transposon Mutagenesis of Mycobacterium marinum Identifies a Locus Linking Pigmentation and Intracellular Survival -- ...
		(B) The location of transposon insertion in mutant MmR03 is shown relative to those in other pigmentation mutants as detected by a PCR that amplifies the genomic sequence between insertions.
		The distance of the transposon insertion in MmW04 from others in the carotenoid biosynthesis locus is insufficiently detected by PCR, and the results were not shown.
		The transposon insertion in MmW04 is not sufficiently
	iai.asm.org /cgi/content/full/71/2/922 (5032 words)

	Large-Scale Mutagenesis of the Yeast Genome Using a Tn7-Derived Multipurpose Transposon -- Kumar et al. 14 (10): 1975 ...
		A multipurpose Tn7-based transposon for mutagenesis of yeast DNA.
		Chromosomal map of sequenced transposon insertions wherein each chromosome is represented by a gray rectangle; chromosomes are split over multiple lines as necessary.
		Stellwagen, A. and Craig, N. Gain-of-function mutations in TnsC, an ATP-dependent transposition protein that activates the bacterial transposon Tn7.
	www.genome.org /cgi/content/full/14/10a/1975 (5886 words)

	Transposon Insertional Mutagenesis in Rice -- Greco et al. 125 (3): 1175 -- PLANT PHYSIOLOGY
		Transposon mutagenesis systems are being developed in rice to address gene functions using forward and reverse genetics strategies.
		Constructs were made with the aim of generating populations of transposon inserts for knockout mutagenesis as well as gene
		Nakagawa Y, Machida C, Machida Y, Toriyama K (2000) Frequency and pattern of transposition of the maize transposable element Ds in transgenic rice plants.
	www.plantphysiol.org /cgi/content/full/125/3/1175 (1383 words)

	biology - Transposon
		Initially retrotransposons copy themselves to RNA (transcription) but, instead of being translated, the RNA is copied into DNA by a reverse transcriptase (often coded by the transposon itself) and inserted back into the genome.
		Transposons in the fruit fly Drosophila melanogaster are called P elements.
		[2] Appropriately, transposons have been an especially useful tool in plant molecular biology.
	www.biologydaily.com /biology/Transposon (971 words)

	Excision of the Conjugative Transposon Tn916 in Lactococcus lactis -- Marra et al. 65 (5): 2230 -- Applied and ...
		Excision of the Conjugative Transposon Tn916 in Lactococcus lactis -- Marra et al.
		Conjugative transposons mediate their own transfer from a donor DNA molecule in one bacterial cell to a target molecule in
		circular transposon molecule that is the intermediate in conjugal
	aem.asm.org /cgi/content/full/65/5/2230 (1584 words)

	Transposon-mediated generation of targeting vectors for the production of gene knockouts -- Zhang et al. 33 (3): e24 -- ...
		to have the transposon 2321 bp upstream of exon 1.
		An advantage that the transposons have over the ET technology is that the vectors are generic and may be used on
		Phadnis, S.H., Huang, H.V., Berg, D.E. (1989) Tn5supF, a 264-base-pair transposon derived from Tn5 for insertion mutagenesis and sequencing DNAs cloned in phage lambda Proc.
	nar.oxfordjournals.org /cgi/content/full/33/3/e24 (4031 words)

	Systematic sequencing of cDNA clones using the transposon Tn5 -- Shevchenko et al. 30 (11): 2469 -- Nucleic Acids ...
		To assess the randomness of insertions of the transposon Tn5
		The general pipeline for the systematic sequencing of cDNA clones using the transposon Tn5 is depicted, with additional details provided in the text.
		Transposon Express, a software application to report the identity of insertions obtained by comprehensive transposon mutagenesis of sequenced genomes: analysis of the preference for in vitro Tn5 transposition into GC-rich DNA
	nar.oxfordjournals.org /cgi/content/full/30/11/2469 (5576 words)

	Random Transposon Mutagenesis of Campylobacter jejuni -- Golden et al. 68 (9): 5450 -- Infection and Immunity
		Genetic studies of Campylobacter jejuni have been limited due to the lack of a transposon mutagenesis method.
		Hyperactive transposase mutants of the Himar1 mariner transposon.
		A purified mariner transposase is sufficient to mediate transposition in vitro.
	iai.asm.org /cgi/content/full/68/9/5450 (1619 words)

	Gene Repair and Transposon-Mediated Gene Therapy -- Richardson et al. 20 (2): 105 -- Stem Cells
		of transposons in gene therapy appears to be inevitable.
		The suppressor-mutator element and the evolutionary riddle of transposons.
		Transposition and gene disruption in the male germline of the mouse.
	stemcells.alphamedpress.org /cgi/content/full/20/2/105 (7170 words)

	GPS®-LS Linker Scanning System, Transposon-based Sequencing/Mutagenesis, New England Biolabs
		Since these transposon donor plasmids are unable to replicate (ori-) in ordinary lab strains of E.
		GPS-LS will also allow secondary insertion of a Tn7-based transposon into a target that has already received one, after Pme I-mediated deletion of the original Transprimer insertion.
		The mutagenesis is accomplished by introduction of a transposon (Transprimer), the majority of which is then removed by restriction digest.
	www.neb.com /nebecomm/products/productE7102.asp (1056 words)

	Global Transposon Mutagenesis and Essential Gene Analysis of Helicobacter pylori -- Salama et al. 186 (23): 7926 -- The ...
		Number of transposon (Tn) insertions per gene weakly correlates with gene size in base pairs.
		The percentage of genes with no transposon insertions in each functional class is indicated.
		found to be essential by mapping of transposon mutants (43).
	jb.asm.org /cgi/content/full/186/23/7926 (6730 words)

	Cancer gene discovery in solid tumours using transposon-based somatic mutagenesis in the mouse : Nature
		Here we report the ability of the Sleeping Beauty transposon to act as a somatic insertional mutagen to identify genes involved in solid tumour formation.
		A Sleeping Beauty transposon, engineered to elicit loss-of-function or gain-of-function mutations, transposed in all somatic tissues tested and accelerated tumour formation in mice predisposed to cancer.
		Cloning transposon insertion sites from these tumours revealed the presence of common integration sites, at known and candidate cancer genes, similar to those observed in retroviral mutagenesis screens.
	www.nature.com /nature/journal/v436/n7048/abs/nature03681.html (286 words)

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