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Patent 6,808,707 The composition comprises virgin monomers of tropoelastin and lysyl oxidase. The notion that tropoelastin monomers are secreted from the cell and diffuse onto the surface of growing fibers seems to be inadequate to explain the efficiency of the whole process and the variable forms of elastic fibers in different tissues. Since tropoelastin monomers are normally secreted from the cell, a preferred way to produce the monomers for the present invention would be to introduce the gene with the signal sequence intact and under the control of a strong promoter into a cell and grow the recombinant cells in culture. www.pharmcast.com /Patents100/Yr2004/Oct2004/102604/6808707_Wound102604.htm   (3325 words)

Microfibril-associated glycoprotein-1 binding to tropoelastin: Multiple binding sites and the role of divalent cations ...   (Site not responding. Last check: 2007-11-02) Following SDS/PAGE, tropoelastin was blotted onto poly(vinylidene difluoride) for a ligand overlay assay with MAGP-1 as the soluble ligand. binding to tropoelastin in the absence of CaCl   Binding of soluble MAGP-1 to tropoelastin in the presence of divalent cations. content.febsjournal.org /cgi/content/full/271/14/3085   (3030 words)

Hydrophobic Domains of Human Tropoelastin Interact in a Context-dependent Manner -- Toonkool et al. 276 (48): 44575 -- ...   (Site not responding. Last check: 2007-11-02) Tropoelastin is the soluble precursor of elastin, the major component of the extracellular elastic fiber. The plots in a and b are representative of the coacervation profiles of all five tropoelastin constructs, albeit the individual construct profiles varied because of differences in coacervation behavior as shown in panel c and Table II. Domain 26 of tropoelastin consists of four nonapeptide repeats of the sequence XGXGXGVPG, where X is either A, L, V, or I. It plays a dominant role in coacervation (15). www.jbc.org /cgi/content/full/276/48/44575   (3389 words)

Respiratory Disorders Program at Brigham and Women's Hospital Faculty Members Elastic fibers, consisting of soluble tropoelastin monomers cross-linked on a preexisting scaffold of microfibrils, are produced primarily during late fetal and neonatal stages of development. The onset and inductive phase of tropoelastin expression are characterized by increased transcription of the tropoelastin gene. We have found in silica-induced pulmonary fibrosis that expression of tropoelastin is primarily increased in the walls and the septal tips of the alveolus, with modest increases in other compartments which normally express tropoelastin during development. www.brighamandwomens.org /respiratorydisorders/Faculty/Mariani.aspx   (3772 words)

Identification of Tropoelastin as a Ligand for the 65-kD FK506-binding Protein, FKBP65, in the Secretory Pathway -- ...   (Site not responding. Last check: 2007-11-02) Identification of Tropoelastin as a Ligand for the 65-kD FK506-binding Protein, FKBP65, in the Secretory Pathway -- Davis et al. Identification of Tropoelastin as a Ligand for the 65-kD FK506-binding Protein, FKBP65, in the Secretory Pathway Bovine FKBP65 is cross-linked to tropoelastin in the secretory pathway of FBCs. www.jcb.org /cgi/content/full/140/2/295   (6436 words)

Hypoxia downregulates tropoelastin gene expression in rat lung fibroblasts by pretranslational mechanisms -- Berk et ...   (Site not responding. Last check: 2007-11-02) Hypoxia downregulates tropoelastin gene expression in rat lung fibroblasts by pretranslational mechanisms -- Berk et al. Optical densities of tropoelastin transcript signals were determined by laser densitometry and normalized to the intensity of the accompanying 18S ribosomal RNA signal. The intensity of tropoelastin mRNA transcripts was quantified by laser densitometry and normalized to 18S ribosomal RNA signal. ajplung.physiology.org /cgi/content/full/277/3/L566   (3557 words)

An Open Reading Frame Element Mediates Posttranscriptional Regulation of Tropoelastin and Responsiveness to ...   (Site not responding. Last check: 2007-11-02) Tropoelastin mRNA is transcribed from 36 exons, which alternate between regions coding for hydrophobic or cross-linking domains. Levels of tropoelastin (TE) and GAPDH mRNAs were assessed by Northern hybridization (upper panels), and tropoelastin mRNA-binding protein (TE mRNA BP) activity was assessed by incubation with labeled exon-30 RNA (lower panels). Tropoelastin gene expression in the rat pulmonary vasculature: a developmental study. mcb.asm.org /cgi/content/full/19/11/7314   (8607 words)

Developmental Regulation of FKBP65. An ER-localized Extracellular Matrix Binding-Protein -- Patterson et al. 11 (11): ...   (Site not responding. Last check: 2007-11-02) When tropoelastin was accumulated in the Golgi, however, the two proteins showed distinct patterns of immunolabeling with tropoelastin in the peri-nuclear Golgi region and FKBP65 remaining in the ER. Davis, E.C., and Mecham, R.P. Intracellular trafficking of tropoelastin. Davis, E.C., Broekelmann, T.J., Ozawa, Y., and Mecham, R.P. Identification of tropoelastin as a ligand for the 65-kD FK506-binding protein, FKBP65, in the secretory pathway. www.molbiolcell.org /cgi/content/full/11/11/3925   (5851 words)

Deficient coacervation of two forms of human tropoelastin associated with supravalvular aortic stenosis -- Wu and Weiss ...   (Site not responding. Last check: 2007-11-02) the tropoelastin gene in supravalvular aortic stenosis (SVAS). of the truncated SVAS tropoelastin molecules was negligible The transition temperatures of coacervation are shown at high and low concentrations, adjusted for molarity in each case, to permit comparison of concentration dependence behaviour of the three tropoelastin forms. content.febsjournal.org /cgi/content/full/266/1/308   (2548 words)

News in Science - Can damaged tissues spring back into shape? - 13/09/2002 Called tropoelastin, these building blocks, like bricks in a brick wall, are cemented together to form elastin. The team first discovered how to make tropoelastin in the mid 1990s and last year was awarded a US patent on the synthesis and cementing process. The shapes are made by placing the tropoelastin 'bricks' and the 'cement' in moulds to determine the shape, and 'cooking' the mixture at body temperature for 10 minutes. www.abc.net.au /science/news/stories/s675466.htm   (598 words)

A critical role for elastin signaling in vascular morphogenesis and disease -- Karnik et al. 130 (2): 411 Figure IG4 -- ...   (Site not responding. Last check: 2007-11-02) Tropoelastin regulates migration and actin polymerization via a non-integrin, G-protein-coupled signaling pathway. Control experiments with the B protomer of pertussis toxin alone demonstrate that the effect of pertussis toxin is specific to its ability to inhibit G-protein signaling. In the presence of tropoelastin and forskolin, there is a marked decrease in cAMP. dev.biologists.org /cgi/content/full/130/2/411/FIG4   (333 words)

Developmental Expression of Latent Transforming Growth Factor beta Binding Protein 2 and Its Requirement Early in Mouse ...   (Site not responding. Last check: 2007-11-02) Expression in the lung is observable in a limited number of cell layers of the subepithelial mesenchyme of developing airways (arrows), and intense tropoelastin expression in arterial vessels (asterisks) is evident. In situ hybridization was done to localize expression of LTBP-2 in the lung (A) and spleen (C) and expression of tropoelastin in the lung (B) and spleen (D). Coexpression of LTBP-2 and tropoelastin is seen throughout the mesenchyme and vasculature of the lung and the capsule of the spleen. mcb.asm.org /cgi/content/full/20/13/4879   (4876 words)

Transcriptional regulation of tropoelastin expression in rat lung fibroblasts: changes with age and hyperoxia -- Bruce ...   (Site not responding. Last check: 2007-11-02) Tropoelastin hnRNA was present in each sample treated with RT but absent in each sample not reverse transcribed, demonstrating absence of contaminating genomic DNA in samples treated with DNase I. Nos. Tropoelastin hnRNA levels, normalized to cyclophilin values, are seen to decrease after postnatal day 2 and to begin to increase again on day 8. Signal for tropoelastin hnRNA and mRNA (pooled fibroblasts from 10-12 fetal lungs at each gestational age and 2-3 lungs at each postnatal age) was amplified by RT-PCR with primer pairs for tropoelastin hnRNA (25 cycles), tropoelastin mRNA (16 cycles), and cyclophilin (18 cycles). ajplung.physiology.org /cgi/content/full/274/6/L940   (7218 words)

Fibroblast Tropoelastin and alpha -Smooth-Muscle Actin Expression Are Repressed by Particulate-Activated ...   (Site not responding. Last check: 2007-11-02) Fibroblast Tropoelastin and alpha -Smooth-Muscle Actin Expression Are Repressed by Particulate-Activated Macrophage-Derived Tumor Necrosis Factor-alpha in Experimental Silicosis -- Mariani et al. Expression of the tropoelastin gene is observed at alveolar septal tips adjacent to granulomas (arrowheads), but no expression is seen within the lesions. The repression of tropoelastin steady-state mRNA levels was barely detectable by 12 h and was maximal at 48 h. ajrcmb.atsjournals.org /cgi/content/full/21/2/185   (3968 words)

Creating unique personal care products scientifically Research has shown that human tropoelastin is in fact isomorphic, and at least 9 different isomorphs of elastin have been identified, including different isomorphic compositions of distinct tissues (Fig 3). The relative abundance of 8 of the tropoelastin exons was measured in human skin fibroblast, placental and fetal aortal tissues. A tropoelastin composition modeled after that of the chosen population group could be produced. www.dermaplusproducts.com /technology3.htm   (385 words)

Hydrophobic domains of human tropoelastin interact in a context-dependent manner - News & events - The University of ... The present work explores some of the properties of the central hydrophobic domains of tropoelastin and their contribution to association in the context of position in the full length molecule. Various tropoelastin constructs, including a naturally occurring form of tropoelastin, its mutant derivatives, and shortened molecules, were studied with respect to their secondary structures and association abilities. These results indicate that the assembly of tropoelastin molecules is not merely a result of random interactions of hydrophobic domains but that it is caused by multiple and specific interactions of individual hydrophobic domains. www.medfac.usyd.edu.au /news/features/2002/020610.php   (396 words)

Mechanism of Abnormal Elastin Gene Expression in the Pinguecular Part of Pterygia -- Wang et al. 157 (4): 1269 -- ...   (Site not responding. Last check: 2007-11-02) The increased expression of tropoelastin in pinguecular and Tropoelastin and glyceraldehyde-3-phosphate dehydrogenase mRNA levels at steady-state and after UV irradiation in conjunctival fibroblasts and pinguecular fibroblasts of pterygia. In the cellular extracts, the amounts of radiolabeled tropoelastin were similar to fibroblasts of normal conjunctiva cells, UVB-irradiated conjunctival fibroblasts, and pinguecular fibroblasts (n = 5; one-way analysis of variance; P > 0.05). ajp.amjpathol.org /cgi/content/full/157/4/1269   (4080 words)

Characterization of an In Vitro Model of Elastic Fiber Assembly -- Robb et al. 10 (11): 3595 -- Molecular Biology of ...   (Site not responding. Last check: 2007-11-02) Tropoelastin (TE) was immunoprecipitated from the cell lysates and media with the use of heat-killed Staphylococcus aureus (Pansorbin cells) to collect the immune complexes. PE cells stably transfected with tropoelastin assemble tropoelastin onto a microfibril scaffold to form elastic fibers in the extracellular matrix. PE cells stably transfected with tropoelastin assemble elastin in the extracellular matrix in a manner similar to that of FBCs. www.molbiolcell.org /cgi/content/full/10/11/3595   (5199 words)

Protein Interaction Studies of MAGP-1 with Tropoelastin and Fibrillin-1 -- Jensen et al. 276 (43): 39661 -- Journal of ...   (Site not responding. Last check: 2007-11-02) of tropoelastin corresponding to the N-terminal, C-terminal, and Positions of the N-terminal and C-terminal fragments of tropoelastin are marked. PVDF membranes with immobilized fibrillin-1 fragment rF23 (lane 1) and tropoelastin (lane 2) were used in ligand overlay assays in the presence or absence of 2 m www.jbc.org /cgi/content/full/276/43/39661   (4600 words)

A critical role for elastin signaling in vascular morphogenesis and disease -- Karnik et al. 130 (2): 411 -- Development   (Site not responding. Last check: 2007-11-02) Tropoelastin mediated actin polymerization is unaffected when Eln vascular smooth muscle cells migrate to tropoelastin in a concentration-dependent manner, indicating that tropoelastin is a specific and direct stimulus for vascular smooth muscle cell localization. A modified Boyden chamber assay was used for all experiments to determine the total number of migrated cells in 15 randomly selected high power microscope fields (HPF). dev.biologists.org /cgi/content/figsonly/130/2/411?ck=nck   (1085 words)

Tropoelastin antibody [T11E3] (ab21598) datasheet   (Site not responding. Last check: 2007-11-02) If Tropoelastin antibody [T11E3] (ab21598) does not perform as described on this datasheet, notify us within 90 days of delivery using this form, so that we can help you or offer you a replacement or a refund. Tropoelastin is a major structural protein of tissues such as aorta and nuchal ligament, which must expand rapidly and recover completely. Molecular determinant of the late arterial morphogenesis, stabilizing arterial structure by regulating proliferation and organization of vascular smooth muscle. www.abcam.com /?datasheet=21598   (409 words)

(WO/2000/073399) METHODS FOR PRODUCING LAMINATED ELASTIN, ELASTIN-BASED MATERIALS AND TROPOELASTIN PRODUCTS FOR ...   (Site not responding. Last check: 2007-11-02) The invention is directed to a method including the steps of providing at least one layer of unlaminated elastin or unlaminated elastin-based materials or unlaminated tropoelastin materials. Then, the unlaminated elastin or unlaminated elastin-based materials or unlaminated tropoelastin materials is subjected to heating and pressing steps. Typically, the step of adhering with an adhesive material the laminated elastin or laminated elastin-based materials or laminated tropoelastin materials is employed in order to achieve a water-tight engagement with the tissue substrate. www.wipo.int /ipdl/IPDL-CIMAGES/view/pct/getbykey5?KEY=00/73399.001207   (347 words)

Tropoelastin antibody (ab21601) datasheet   (Site not responding. Last check: 2007-11-02) This product reacts most strongly with mouse tropoelastin and less strongly with insoluble forms of elastin. If Tropoelastin antibody (ab21601) does not perform as described on this datasheet, notify us within 90 days of delivery using this form, so that we can help you or offer you a replacement or a refund. Store at +4°C. At Abcam, we have one centralized database to hold all of our product information, so that everything we know about this Tropoelastin antibody is on this datasheet. www.abcam.com /?datasheet=21601   (402 words)

Elastogenesis in Human Arterial Disease: A Role for Macrophages in Disordered Elastin Synthesis -- Krettek et al. 23 ...   (Site not responding. Last check: 2007-11-02) A, Representative Western blot shows tropoelastin protein levels in tissue extracts (50 µg protein) from nonatherosclerotic arteries (normal, n=8), fibrous plaques (n=7), atheromatous plaques (n=9), and AAA (n=7) developed with antibody E4013. tropoelastin expression in macrophages in foam cell-rich lesions Hinek A, Rabinovitch M. 67-kD elastin-binding protein is a protective "companion" of extracellular insoluble elastin and intracellular tropoelastin. atvb.ahajournals.org /cgi/content/full/23/4/582   (3450 words)

Action of Tropoelastin and Synthetic Elastin Sequences on Vascular Tone and on Free Ca2+ Level in Human Vascular ...   (Site not responding. Last check: 2007-11-02) Action of Tropoelastin and Synthetic Elastin Sequences on Vascular Tone and on Free Ca2+ Level in Human Vascular Endothelial Cells -- Faury et al. Action of Tropoelastin and Synthetic Elastin Sequences on Vascular Tone and on Free Ca Level in Human Vascular Endothelial Cells Kinetics of receptor-mediated binding of tropoelastin to ligament fibroblasts. intl-circres.ahajournals.org /cgi/content/full/82/3/328   (4122 words)

Elastin & Related antibodies from RDI Divison of Fitzgerald Industries Intl Whole sascites fluid from mice immunized wth a hybridoma producing antibody to bovive ligamentum nuchae tropoelastin. Whole serum obtained from rabbits immunized with a bacterial fusion protein containing protein encoded by exons 31-36 of mouse tropoelastin. Whole ascities fluid from mice immunized with a hybridoma-producing antibody to bovine ligamentum nuchae tropoelastin. www.researchd.com /rdiabs/elastin.htm   (1197 words)

Effects of Monensin on Tropoelastin Metabolism in Vascular Smooth Muscle Cells: Monensin Causes Intracellular ...   (Site not responding. Last check: 2007-11-02) Effects of Monensin on Tropoelastin Metabolism in Vascular Smooth Muscle Cells: Monensin Causes Intracellular Degradation of Accumulated Tropoelastin -- Ikeda et al. reduction of tropoelastin synthesis from 18 to 48 h to one-tenth. that half of the accumulated tropoelastin in the cells was rapidly jb.oxfordjournals.org /cgi/content/abstract/121/1/5   (187 words)

Quantitation of tropoelastin mRNA and assessment of alternative splicing in human skin fibroblasts by reverse ...   (Site not responding. Last check: 2007-11-02) Quantitation of tropoelastin mRNA and assessment of alternative splicing in human skin fibroblasts by reverse transcriptase-polymerase chain reaction -- Holzenberger et al. Quantitation of tropoelastin mRNA and assessment of alternative splicing in human skin fibroblasts by reverse transcriptase-polymerase chain reaction PCR) assay for the quantitative measurement of levels of tropoelastin mRNA www.genome.org /cgi/content/abstract/3/2/107   (197 words)

(WO/2000/044394) METHOD OF PRODUCING ELASTIN, ELASTIN-BASED MATERIALS AND TROPOELASTIN MATERIALS   (Site not responding. Last check: 2007-11-02) A method is provided for producing elastin or elastin-based materials or tropoelastin materials which have a substantially reduced level of calcification. These materials are typically capable of being formed into a fused layer. The subject method comprises providing unreacted elastin or elastin-based biomaterials or tropoelastin materials, and pretreating the unreacted elastin or elastin-based biomaterials or tropoelastin materials with an aliphatic alcohol prior to reaction thereof. www.wipo.int /ipdl/IPDL-CIMAGES/view/pct/getbykey5?KEY=00/44394.001102   (244 words)

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