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Topic: Ultracentrifuge

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	Angular Speed of a Medical Centrifuge
		The ultracentrifuge is widely used in the study of polymers, proteins, nucleic acids, viruses, and other organic macromolecules.Ultracentrifuges are used in biological and chemical laboratories and in medicine to prepare serums and plasma from blood.
		The ultracentrifuge is balanced on a cushion of air and spins because a jet of compressed air touches the outer surface.
		A rotor of a typical ultracentrifuge is eighteen centimeters in diameter and carries ten ounces of liquid in a centrifugal field.
	hypertextbook.com /facts/ArikaBryant.shtml (378 words)

	Ultracentrifuge Explosion Damages Laboratory :: News :: Laboratory Safety @ EHRS
		On December 16, 1998, milk samples were running in a Beckman L2-65B ultracentrifuge using a large aluminum rotor (a rotor is a large metal object that holds the individual sample tubes and is connected to the spin drive of the centrifuge).
		The cause of the accident is believed to be the use of a model of rotor that was not approved by Beckman for use in a model L2-65B ultracentrifuge.
		It is critical that all safety and maintenance procedures specified by the manufacturer are followed.
	www.ehrs.upenn.edu /programs/labsafety/ultra_explosion.html (737 words)

	Cell (biology) - Printer-friendly - MSN Encarta
		The ultracentrifuge is an instrument that spins cells or other substances in test tubes at high speeds, which causes the heavier parts of the substance to fall to the bottom of the test tube.
		This instrument enabled scientists to separate the relatively abundant and heavy mitochondria from the rest of the cell and study their chemical reactions.
		Using refined techniques with the ultracentrifuge, scientists subsequently isolated the smaller organelles and gained an understanding of their functions.
	encarta.msn.com /text_761568585___52/Cell_(biology).html (913 words)

	Sucrose Gradients (Site not responding. Last check: 2007-10-12)
		Great: pick lines that are flowing well, stick them in the ultracentrifuge tubes, and open the mixer between the two sucrose solutions (make sure you see high-concentration sucrose being whipped into the outlet side of the gradient maker).
		If the levels are uneven, adjust the relative heights of the ultracentrifuge tubes (i.e., lower the slower-forming gradient) to re-balance the levels.
		Carefully insert the long blunt-end needle into an ultracentrifuge tube containing a sucrose gradient, ensuring the needle rests at the bottom of the tube.
	web.mit.edu /king-lab/www/cookbook/psucrose.htm (654 words)

	Ultracentrifuge - Wikipedia, the free encyclopedia
		The ultracentrifuge is a centrifuge optimized for spinning a rotor at very high speeds, capable of generating acceleration as high as 1,000,000 G (9,800 km/s²).
		Theodor Svedberg invented the analytical ultracentrifuge in 1923, and won the Nobel Prize in Chemistry in 1926 for his research on colloids and proteins using the ultracentrifuge.
		This experiment is insensitive to the shape of the macromolecule, and directly reports on the molar mass of the macromolecules and, for chemically reacting mixtures, on chemical equilibrium constants.
	en.wikipedia.org /wiki/Ultracentrifuge (743 words)

	Theodor Svedberg Summary
		Svedberg's development of the ultracentrifuge to study solutions was of enormous importance to biologists, who believed that gaining an understanding of colloids would help them to create models of biological systems.
		This early ultracentrifuge was elaborate, equipped with both a camera and illumination for photographing samples during centrifugation.
		He extended his ultracentrifuge studies of large carbohydrate molecules, combining his interest in biomolecules with his interest in botany by undertaking a pioneering study of the complex sugars of the Lillifloreae family, which includes lilies and irises.
	www.bookrags.com /Theodor_Svedberg (3324 words)

	Centrifuge Explosion Damages Laboratory
		A campus laboratory was seriously damaged when the rotor of an ultracentrifuge failed while in use.
		On December 16, 1998, milk samples were running in a Beckman.L2-65B ultracentrifuge using a large aluminum rotor (a rotor is a large metal object that holds the individual sample tubes and is connected to the spin drive of the centrifuge).
		This includes an orange decal on the sliding door that specifies the rotor models that are safe to use in a particular unit (picture).
	www.ehs.cornell.edu /lrs/Centrifuge/CentrifugeDamages.htm (936 words)

	Analytical Ultracentrifugation as a Contemporary Biomolecular Research Tool
		Analytical ultracentrifugation has again become a widely used biomolecular research technique for determining sample purity, characterizing assembly and disassembly mechanisms of biomolecular complexes, determining subunit stoichiometries, detecting and characterizing macromolecular conformational changes, and measuring equilibrium constants and thermodynamic parameters for self- and hetero-associating systems.
		An analytical ultracentrifuge can be thought of as a marriage between a preparative ultracentrifuge and an optical detection system that is capable of directly measuring the sample concentration inside the centrifuge cell during sedimentation.
		When analyzing a sample by analytical ultracentrifugation for the first time, it is strongly recommended to use both sedimentation velocity and sedimentation equilibrium approaches to survey the behavior of the biomolecule over a broad range of sample concentrations, rotor speeds, or other experimental conditions.
	www.abrf.org /JBT/1999/December99/dec99cole.html (7298 words)

	Fact Sheet: Ultracentrifuges: Hazards and Precautions (Site not responding. Last check: 2007-10-12)
		The ultracentrifuge is a commonly used type of laboratory equipment.
		To prevent such failures, all ultracentrifuge manufacturers require that ultracentrifuge users maintain an up-to-date use log for each rotor, and also require that each time a rotor is used it must be given a thorough visual inspection for any signs of damage or weakness.
		A standard 20-pound fixed chamber ultracentrifuge rotor at these conditions holds over a million joules of energy, which is roughly equivalent to the energy released by exploding several 1” sticks of dynamite.
	www.ehs.berkeley.edu /pubs/factsheets/56ultracentrifuges.html (615 words)

	Ultracentrifuge Abstract
		The ultracentrifuge is an important bioanalytical instrument that has long been used for determining the molecular weight and physical characteristics of proteins in solution.
		The ultracentrifuge's photomultiplier tube has been directly linked to a CAMAC A/D converter, allowing characterization of the reference and sample intensity peaks.
		The output from the photomultiplier is collected while peak heights are averaged at each radius until the signal to noise ratio reaches a predetermined level.
	mywebpages.comcast.net /lilgerry/Ultracentrifuge_Extended_Abstract.htm (253 words)

	Salmon Lab Protocols: Rat Liver Cytosol Prep
		Ultracentrifuge (Beckman L7-55) with the equivalents of Beckman 50.2Ti and SW-28 rotors
		Chill the superspeed centrifuge, ultracentrifuge, SS-34 and 50.2Ti rotor to 4oC.
		Separate the organelles by density by centrifuging the gradient at 100,000 x g (28,000 rpm) for 3 h at 4oC in a SW-28 rotor.
	www.bio.unc.edu /faculty/salmon/lab/protocolsratlivercytosol.html (703 words)

	Centrifuge tube having removable crown and swage fitting - Patent 4537320
		A centrifuge tube for use in an ultracentrifuge has a closed bottom end shaped to mate with the bottom of a rotor cavity, a body portion and an upper end that terminates in a filler stem.
		The present invention is directed to centrifuge tubes and associated sealing means for use with ultracentrifuge apparatus, and more particularly, relates to a thin wall, flexible centrifuge tube and associated sealing mechanism.
		In providing centrifuge tubes for use in the rotors of ultracentrifuge chambers it is important to offer multiple sample utilization, ease of use, a maximum fill of the tube, means of eliminating air bubbles, access to sample before and after centrifugation, universality (use in all sizes of rotors) and noncontamination.
	www.freepatentsonline.com /4537320.html (6069 words)

	Winter 1999 Newsletter
		In the event of operating problems with high-speed centrifuge or ultracentrifuge units, or signs of wear or damage to rotors, the equipment should be taken out of service immediately and clearly marked "Warning -- DO NOT USE" until checked by an authorized service representative.
		Based on the investigation of the December accident, EHandS learned that Beckman L2 and L3 series ultracentrifuges have special operating procedures and restrictions to reduce the risk of damage and injuries.
		This includes an orange decal on the sliding door that specifies the rotor models that are safe to use in a particular unit.
	www.ehs.cornell.edu /lrs/news/jan99.htm (1019 words)

	UMMC AUF Web Page
		The Analytical Ultracentrifuge Facility is located in the laboratory of Dr. Jack Correia (seen hovering in the background above) in the Department of Biochemistry, G208-G209 in the Guyton Research Building.
		In addition any prior ultracentrifuge or biophysical work done on this system should be referenced.
		If a 1% contaminant has an E that is 10 times larger than the 99% pure band, then the XLA will "see" it as 10% of the material, etc. Thus, each sample should be accompanied by a gel to access purity.
	biochemistry.umc.edu /ummcauf.html (1198 words)

	Keck Facility- XLA-70 Analytical Ultracentrifuge
		The analytical ultracentrifuge allows you to measure many important properties of macromolecules and complexes in solution, including:
		A nice feature of the instrument, made possible by its ability to monitor at multiple wavelengths, is that one can separately follow multiple chromophores, e.g., both a macromolecule and, separately, a ligand.
		The 4-hole rotor holds 3 cells and can be used for velocity experiments (2-sector cells, each cell holds one sample and one reference solution) and equilibrium experiments (2- or 6-sector cells, which hold 3 samples and 3 reference solutions).
	www.biochem.northwestern.edu /Keck/instruments/FM-xla.html (272 words)

	Cell Biology Problems (Site not responding. Last check: 2007-10-12)
		Biochemical study of subcellular components like ribosomes, endoplasmic reticulum, microsomes etc., was greatly facilitated in the early 1940s with the commercial availability of the instrument known as the preparative ultracentrifuge.
		The preparative ultracentrifuge allowed fractionation of subcellular particles from a homogenate of tissue by centrifuging at different speeds.
		You obtain a piece of fresh bovine liver from a nearby slaughter house and homogenize it by using your room mate's fruit juice blender.
	web.mit.edu /esgbio/www/cb/problems.html (1477 words)

	Home Page
		SETON SCIENTIFIC was founded in 1983 to provide an independent, alternate source of lower cost, high quality tubes, bottles and accessories.
		Ultracentrifuge tube manufacturing in SETON's modern U.S.A. facility requires adherence to strict quality control methods and the use of specialized resin formulas for the best tube performance.
		Please read your rotor instruction manual before using any ultracentrifuge tubes, so that you may select the correct caps, if needed, and note the safe speeds, fill levels, temperatures, and chemical compatibilities.
	www.setonscientific.com (90 words)

	RASMB
		Storrs 2006: 15th Annual Workshop on Analytical Ultracentrifugation at the University of Connecticut
		The Analytical Ultracentrifuge Facility is located in the laboratory of Dr. Jack Correia, Department of Biochemistry, Jackson, MS.
		The Analytical Ultracentrifugation Research Laboratory at the BBRI.
	www.bbri.org /RASMB/rasmb.html (364 words)

	[No title]
		The ultracentrifuge is a Beckman L8-M with a 70Ti rotor.
		Sign up for Sorvall centrifuge and for ultracentrifuge 2.
		Put rotor for ultracentrifuge in cold room to chill overnight 3.
	www.tufts.edu /~mcourt01/Documents/Hepatic_microsomes_protocol.doc (453 words)

	Alfa Wassermann Separation Technologies
		Alfa Wassermann Separation Technologies (AWST) provides solutions for process development and industrial scale manufacturing to efficiently and reliably separate viruses in the development and manufacture of vaccines and other bioproducts.
		Our continuous flow ultracentrifuges are used for protocol development and small scale production, PKII, as well as industrial scale production with our KII.
		Our qualified Service and Application staff will be available to discuss any questions you may have, including topics like application development, aseptic processing, cleaning and validation of the Ultracentrifuge.
	www.awst.com /index.html (172 words)

	Macro Molecular
		Because of its ability to quantitate the size and shape of macromolecules in solution, one of the most powerful methods for characterization of macromolecular interactions is Solution Interaction Analysis (sIA).
		Sedimentation velocity or sedimentation equilibrium experiments are used to determine Stoichiometry and Shape, Equilibrium Constants, Sedimentation Coefficients, Diffusion Coefficients, and Apparent Weight Average Molecular Weights of proteins, DNA, RNA etc.
		No labeling of the interacting components is required and the technology is applicable to a wide range of experimental situations.
	www.icmb.utexas.edu /core/Protein/MacroMolecular.htm (216 words)

	Thermodynamic and Hydrodynamic Properties of Human Tropoelastin. ANALYTICAL ULTRACENTRIFUGE AND PULSED FIELD-GRADIENT ...
		Computer Modeling-- Computer modeling was used to address two questions arising from the ultracentrifuge data.
		Ralston, G. and Morris, M. in Analytical Ultracentrifugation in Biochemistry and Polymer Science (Harding, S., Rowe, A., and Horton, J. C., eds), pp.
		Laue, T. Shah, B. Ridgeway, T. and Pelletier, S. in Analytical Ultracentrifugation in Biochemistry and Polymer Science (Harding, S., Rowe, A., and Horton, J. C., eds), pp.
	www.jbc.org /cgi/content/full/276/30/28042 (6005 words)

	Amazon.com: Ultracentrifuge: Books: Theodor Svedberg,Kai O. Pedersen (Site not responding. Last check: 2007-10-12)
		Ultracentrifuge — Reasonable pricing, fast shipping and dedicated customer service.
		Ultracentrifuge at GlobalSpec.com — Find the exact Ultracentrifuge your application requires through SpecSearch, a powerful parametric search tool.
		New & Used Laboratory Centrifuges — Thousands of name brand centrifuges to choose from with discounts up to 70% off.
	www.amazon.com /Ultracentrifuge-Theodor-Svedberg/dp/0384588905 (512 words)

	Ultracentrifuge for separating fluid mixtures - Patent 3955757
		Ultracentrifuge for separating fluid mixtures - Patent 3955757
		Use our search engine to find what you need
		While particular embodiments of the invention have been illustrated and described herein, it is not intended that the invention be limited to such embodiments, but changes and modifications may be made and incorporated herein within the spirit of the invention and the scope of the appended claims.
	www.freepatentsonline.com /3955757.html (2355 words)

	Beckman Optima TL 100 ultracentrifuge (Site not responding. Last check: 2007-10-12)
		Sometimes it is necessary to be flexible with your rotor choice without compromising your application
		This state-of-the-art ultracentrifuge is able to reliably serve your lab for as long as you'll be there.
		Beckman's' unsurpassed Optima series technology includes a vacuum encased induction drive- the first of its kind in the world.
	www.gmi-inc.com /Products/beckmantl100.htm (293 words)

	Ultracentrifuge Literature List
		A Microscale Method for Isolating Plasmid DNA in the TL-100 Tabletop Ultracentrifuge
		A rapid Method for Isolating Plasmid DNA in the TL-100 Tabletop Ultracentrifuge
		Preparation DNA from Bacteriophage Lambda Isolated in the TL-100 Tabletop Ultracentrifuge
	www.beckman.com /resourcecenter/literature/BioLit/BioLitList.asp?ProductCategoryID=UCNT (468 words)

	Centrifuge - Floor - New Surplus Refurbished Used Equipment
		Centrifuge, Floor, Beckman, Model L8-55R Ultracentrifuge, Refrigerated, High-speed vacuum.
		Serial Number 51342 SPEED-Speed Range-Speed Control-Speed Display 1,000 to 55,000 rpmActual rotor speed will be within 1% of the set speedDigital r...
		We are looking for a Sorvall RC-5B Floor Model Centrifuge.
	www.labx.com /atlabx/allcats/Centrifuge-Floor.cfm (638 words)

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