| | Single-tube DNA Purification and Cloning Using Ultrafiltration Devices (Site not responding. Last check: 2007-10-11) |
 | | To determine whether ultrafiltration damages or contaminates DNA samples, experiments to test the ability of the DNA to be cleaved by a variety of restriction enzymes were performed. |
 | | In this report we demonstrate that ultrafiltration with Nanosep devices is well suited for the high-yield, rapid recovery of dilute DNA samples without the retention of components that may inhibit biological activity. |
 | | Although ultrafiltration has been shown previously to be an efficient method to purify nucleic acids (2, 4), to our knowledge this is the first report showing the efficacy of ultrafiltration in practical cloning procedures. |
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