Vacuoles and their contents are considered to be distinct from the cytoplasm, and are classified as ergastic according to some authors (Esau, 1965).
Another function of a central vacuole is that it pushes all contents of the cell'scytoplasm against the cellular membrane, and thus keeps the chloroplasts closer to light.
In budding yeastcells, vacuoles are the storage compartments of amino acids and the detoxification compartment, which are described in previous topics.
The single large vacuole of the cell is surrounded by a membrane, called the tonoplast, and filled with a solution of water, dissolved ions, sugars, amino acids, and other materials.
Expansion of the vacuole by water intake is the major driving force in plant cell growth, and is also the means for maintaining cell rigidity, or turgor.
In a mature cell, the vacuole may occupy as much as 90 percent of the cell volume, such that the rest of the cell contents are flattened against the cell membrane.
Vacuoles and their contents are considered to be distinct from the cytoplasm, and are classified as ergastic according to some authors.
Vacuoles also play a major role in autophagy, maintaining an inbalance between biogenesis (production) and degradation (or turnover), of many substances and cell structures.
Note the proliferation of small vacuoles around the periphery of the secretory cell and their apparent association with the plasmalemma at the wall extension areas.
Mitchell HJ, Hardham AR (1999) Characterization of the water expulsion vacuole in Phytophthora nicotianae zoospores.
Verbelen JP, Tao W (1998) Mobile arrays of vacuole ripples are common in plant cells.
Intervacuole vesicular exchange and the segregation of parental vacuole material into the bud are strikingly impaired in a temperature-sensitive yeast mutant, vac1-1.
In zygotes, vacuoles from a vac1-1 strain are defective as donors, or recipients, of the vesicles of intervacuole vesicular traffic.
These defects are specific for the vacuole in that the segregation of nuclear DNA and of mitochondria into the bud appears normal.
A truncated form of the Pho80 cyclin redirects the Pho85 kinase to disrupt vacuole inheritance in S. cerevisiae -- Nicolson et al.
Bryant, N. J., Stevens, T. VacuoleBiogenesis in Saccharomyces cerevisiae: Protein Transport Pathways to the YeastVacuole.
Pan, X, Goldfarb, D. YEB3/VAC8 encodes a myristylated armadillo protein of the Saccharomyces cerevisiae vacuolar membrane that functions in vacuole fusion and inheritance.
Identification of a cytoplasm to vacuole targeting determinant in aminopeptidase I -- Oda et al.
Identification of a cytoplasm to vacuole targeting determinant in aminopeptidase I
Silles, E., Mazon, M. J., Gevaert, K., Goethals, M., Vandekerckhove, J., Leber, R., Sandoval, I. Targeting of Aminopeptidase I to the YeastVacuole Is Mediated by Ssa1p, a Cytosolic Member of the 70-kDa Stress Protein Family.
