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Topic: Vimentin


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In the News (Thu 20 Jun 19)

  
  Chicken Polyclonal Antibody to Vimentin from EnCor Biotechnology Inc.
Vimentin is also found in many kinds of cells in tissue culture and in developing neuronal and astrocytic precursor cells in the central nervous system.
Antibodies to vimentin are useful in studies of stem cells and generally to reveal the filamentous cytoskeleton.
Vimentin is expressed alone in fibroblastic and endothelial cells, which are the flattened cells in the middle of the imate which appear green.
www.encorbio.com /cpolyclonal/CPCA-Vim.htm   (369 words)

  
 Vimentin antibody [RV202] - Neural Stem Cell Marker (ab8978) datasheet
Vimentin has been implicated to be involved in the rate of steroid synthesis via its role as a storage network for steroidogenic cholesterol containing lipid droplets.
Vimentin phosphorylation by a protein kinase causes the breakdown of intermediate filaments and activation of an ATP and myosin light chain dependent contractile event.
Vimentin antibodies are thus of value in the differential diagnosis of undifferentiated neoplasms and malignant tumors.
www.abcam.com /index.html?datasheet=8978   (640 words)

  
 Vimentin Biochem Kit™ (BK020)
The Vimentin Biochem Kit™ is an extremely quick and economical way to become familiar with the properties of vimentin protein.
It allows researchers to work with vimentin in the tetrameric (unpolymerized form) and/or the polymeric filamentous form.
Vimentin was polymerized according to the protocol in kit BK020 and visualized with transmission electron microscopy (Fig.
www.cytoskeleton.com /products/biochem/bk020.html   (92 words)

  
  The Raf-1 kinase associates with vimentin kinases and regulates the structure of vimentin filaments -- JANOSCH et al. ...
Soluble vimentin was precipitated with the V9 (DAKO) monoclonal antibody.
Vimentin was phosphorylated with CK2 or GST-Raf-1 in the presence of absence of heparin and analyzed by phosphopeptide mapping.
in fibroblasts induces the rearrangement of the vimentin scaffold.
www.fasebj.org /cgi/content/full/14/13/2008   (7282 words)

  
 Vimentin-dependent utilization of LDL-cholesterol in human adrenal tumor cells is not associated with the level of ...
Cultures of SW-13 cells that spontaneously express human vimentin (cl.1), the transfectant cell lines (T3M, T4M, and T19M), and the parental IF-free cl.2 cells were treated with 50 µg/ml LDL for 24 h.
of the expression of the vimentin transgene (6).
(6), this correlates with vimentin expression and not with
www.jlr.org /cgi/content/full/40/8/1440   (7588 words)

  
 Vimentin protein (V01)
Vimentin is an excellent substrate for protein kinases.
Recombinant Syrian hamster vimentin has been expressed in and purified from a bacterial expression system.
Vimentin is also available in a Vimentin Biochem Kit™ (BK020).
www.cytoskeleton.com /products/if/v01.html   (150 words)

  
 Identification of an autoantigen on the surface of apoptotic human T cells as a new protein interacting with ...
Indicated amounts of vimentin were spotted on a PVDF membrane and incubated with hGIIA (0.1 µg/mL) in the absence (top panel) or the presence (bottom panel) of heparin (50 µg/mL).
Vimentin head (1), rod (2), and tail (3) domains were separated by SDS-PAGE and transferred to a PVDF membrane.
Vimentin exposed on activated platelets and platelet microparticles localizes vitronectin and plasminogen activator inhibitor complexes on their surface.
www.bloodjournal.org /cgi/content/full/102/8/2901   (6746 words)

  
 vimentin Antibody Review
Dako antibody to vimentin (clone V9) was used in immunocytochemistry to investigate the expression and regulation of growth-regulated oncogene alpha in human endometrial stromal cells
Dako Diagnostics mouse vimentin antibody (M7020) was used in immunohistochemistry to study activation of enteric neurons and enteric glia in the guinea pig ileum and colon by IL1B
Dako vimentin antibody was used in immunohistochemistry to study SFTPC gene mutation in an interstitial lung disease patient
www.exactantigen.com /review/VIM.html   (1372 words)

  
 Aurora-B Regulates the Cleavage Furrow-specific Vimentin Phosphorylation in the Cytokinetic Process -- Goto et al. 278 ...
Fragmentation of Phosphorylated Vimentin-- Vimentin (150 µg) was phosphorylated by GST-Aurora-B WT (10 µg) at 25 °C for 30 min in the reaction mixture (1 ml) as described
As shown in A, vimentin phosphorylated by Aurora-B WT was treated with (lane 2) or without (lane 1) lysyl-endopeptidase.
E, vimentin (a and c), S71E (b), and S72E (e) were phosphorylated by Aurora-B (a and b) or Rho-kinase (c and d), digested with trypsin, fractionated by reverse-phase HPLC, and analyzed as described in B.
www.jbc.org /cgi/content/full/278/10/8526   (3725 words)

  
 Amino-terminal Polypeptides of Vimentin Are Responsible for the Changes in Nuclear Architecture Associated with Human ...
of mouse vimentin (vim) residues 22 to 44 (i.e., vim
Sarria, A.J., Nordeen, S.K., and Evans, R.M. Regulated expression of vimentin cDNA in cells in the presence and absence of a preexisting vimentin filament network.
Wang, Q., Shoeman, R., and Traub, P. Identification of the amino acid residues of the amino terminus of vimentin responsible for DNA binding by enzymatic and chemical sequencing and analysis by MALDI-TOF.
www.molbiolcell.org /cgi/content/full/12/1/143   (7289 words)

  
 Association between Keratin and Vimentin Expression, Malignant Phenotype, and Survival in Postmenopausal Breast Cancer ...   (Site not responding. Last check: 2007-10-30)
for keratin and vimentin was assessed semiquantitatively, as
Vimentin immunopositivity was seen in 25 of 53 cases (47%).
Raymond W. A., Leong A. Co-expression of cytokeratin and vimentin intermediate filament proteins in benign and neoplastic breast epithelium.
clincancerres.aacrjournals.org /cgi/content/full/5/10/2698   (3199 words)

  
 VIMENTIN antibodies from RDI Division of Fitzgerald Industries Intl
Due to an aa substitution at position of aa 353 in murine vimentin (that could explain for the weak cross-reaction of the antibody with murine vimentin) they were able to narrow down the binding region around position 353.
These findings were confirmed by truncation mutagenesis experiments using human vimentin (Rogers et al., 1995).
In comparative analyses, VIM 3B4 has turned out to be the most avid monoclonal antibody to vimentin.
www.researchd.com /rdiabs/pro61013.htm   (512 words)

  
 Actin-binding domain of mouse plectin: Crystal structure and binding to vimentin -- Sevcík et al. 271 (10): 1873 ...   (Site not responding. Last check: 2007-10-30)
with vimentin was observed in cultured macrophages [20].
  Overlay of recombinant vimentin fragments with plectin ABD.
ABD to a fragment of vimentin comprising the rod and its N-terminal
content.febsjournal.org /cgi/content/full/271/10/1873   (6165 words)

  
 ZBP-89 represses vimentin gene transcription by interacting with the transcriptional activator, Sp1 -- Zhang et al. 31 ...
indeed reverse the repression caused by ZBP-89 with the vimentin
Pazanin,L., Poljakovic,Z. and Palic,J. (1992) Vimentin and glial fibrillary acidic protein expression in relation to neoplastic cell differentiation in glial tumors.
Carey,I. and Zehner,Z.E. (1995) Regulation of chicken vimentin gene expression by serum, phorbol ester and growth factors: identification of a novel fibroblast growth factor-inducible element.
nar.oxfordjournals.org /cgi/content/full/31/11/2900   (8205 words)

  
 Cutting Edge: Integration of Human T Lymphocyte Cytoskeleton by the Cytolinker Plectin -- Brown et al. 167 (2): 641 -- ...
with the redistribution of vimentin and plectin (Fig.
Plectin colocalizes with vimentin in resting PBT and redistributes to the uropod during polarization.
Rigidity of circulating lymphocytes is primarily conferred by vimentin intermediate filaments.
www.jimmunol.org /cgi/content/full/167/2/641   (3198 words)

  
 The 3' untranslated region of human vimentin mRNA interacts with protein complexes containing eEF-1{gamma} and HAX-1 -- ...
P-labeled vimentin RNA (37/147) (300 000 d.p.m.) was incubated
HeLa WCE (20 µg) was incubated with the respective RNAs in lanes 2 and 4 whereas RNA alone was analyzed in lanes 1 and 3.
P-labeled RNA from the 3'UTR of human vimentin mRNA from position –37 to –123 downstream of the stop codon (37/123) was incubated alone (lane 1) or with increasing concentrations, 1 µg (lane 2), 2 µg (lane 3) or 4 µg (lane 4), of HeLa WCE.
nar.oxfordjournals.org /cgi/content/full/30/23/5017   (6967 words)

  
 Development- and Differentiation-Dependent Reorganization of Intermediate Filaments in Fiber Cells -- Blankenship et ...
vimentin and GFAP are detected in the lens.
vimentin presence at the membrane throughout the elongation process.
The precise role of vimentin in the biology of the lens is not
www.iovs.org /cgi/content/full/42/3/735   (5769 words)

  
 Rigidity of Circulating Lymphocytes Is Primarily Conferred by Vimentin Intermediate Filaments -- Brown et al. 166 (11): ...
Rigidity of Circulating Lymphocytes Is Primarily Conferred by Vimentin Intermediate Filaments -- Brown et al.
the contribution of vimentin filaments to the rigidity of spherical
Site-specific phosphorylation induces disassembly of vimentin filaments in vitro.
www.jimmunol.org /cgi/content/full/166/11/6640   (4652 words)

  
 Reduction of Renal Mass Is Lethal in Mice Lacking Vimentin . Role of Endothelin-Nitric Oxide Imbalance -- Terzi et al. ...
Vimentin is a class III intermediate filament protein mainly expressed in mesenchyme-derived cells including endothelial and
Since vimentin is strongly expressed in normal kidneys, in both vessels and glomeruli, we evaluated, in a previous work (15),
The details of targeted inactivation of vimentin gene in mice have been reported previously (14).
www.jci.org /cgi/content/full/100/6/1520   (5242 words)

  
 Overexpression of Vimentin: Role in the Invasive Phenotype in an Androgen-independent Model of Prostate Cancer -- Singh ...
Overexpression of Vimentin: Role in the Invasive Phenotype in an Androgen-independent Model of Prostate Cancer -- Singh et al.
A 1.8-kb vimentin cDNA fragment was excised from the pCMV-SPORT6
Motility was not affected by transfection of antisense vimentin or vimentin in CL1 or LNCaP cells, respectively.
cancerres.aacrjournals.org /cgi/content/full/63/9/2306   (4541 words)

  
 Association of Pasteurella multocida Toxin with Vimentin -- Shime et al. 70 (11): 6460 -- Infection and Immunity
vimentin was further found to be responsible for the binding
(A) Purified mouse vimentin (1.5 µg) was subjected to the ligand overlay assay with PMT.
Vimentin associated with the beads was extracted by boiling in SDS-PAGE buffer and subjected to Western blot analysis with anti-vimentin antibody as described in the text.
iai.asm.org /cgi/content/full/70/11/6460   (2534 words)

  
 Histamine stimulates phosphorylation of adherens junction proteins and alters their link to vimentin -- Shasby et al. ...
vimentin that immunoprecipitated with VE-cadherin by 50 ± 6%.
vimentin and actin exist as multimeric proteins, it was not possible
Vimentin dephosphorylation by protein phosphatase 2A is modulated by the targeting subunit B55.
ajplung.physiology.org /cgi/content/full/282/6/L1330   (5084 words)

  
 Effects of Intermediate Filaments on Actin-Based Motility of Listeria monocytogenes -- Giardini and Theriot 81 (6): ...
Vimentin is the predominant intermediate filament protein expressed in fibroblasts (Franke and Moll, 1987
angular velocities for bacterial populations in vimentin +/+ and
Tetracycline regulated expression of vimentin in fibroblasts derived from vimentin null mice.
www.biophysj.org /cgi/content/full/81/6/3193   (6008 words)

  
 High-Throughput Tissue Microarray Analysis to Evaluate Genes Uncovered by cDNA Microarray Screening in Renal Cell ...
The dot for the vimentin gene is circled.
Waldherr R, Schwechheimer K: Co-expression of cytokeratin and vimentin intermediate-sized filaments in renal cell carcinomas: comparative study of the intermediate-sized filament distribution in renal cell carcinomas and normal human kidney.
Beham A, Ratschek M, Zatloukal K, Schmid C, Denk H: Distribution of cytokeratins, vimentin and desmoplakins in normal renal tissue, renal cell carcinomas and oncocytoma as revealed by immunofluorescence microscopy.
ajp.amjpathol.org /cgi/content/full/154/4/981   (3831 words)

  
 Vimentin and CP49/filensin form distinct networks in the lens which are independently modulated during lens fibre cell ...
Vimentin and CP49/filensin form distinct networks in the lens which are independently modulated during lens fibre cell differentiation -- Sandilands et al.
Vimentin and CP49/filensin form distinct networks in the lens which are independently modulated during lens fibre cell differentiation
The vimentin network was present in both epithelial cells and
jcs.biologists.org /cgi/content/abstract/108/4/1397   (662 words)

  
 Generation of human cortical neurons from a new immortal fetal neural stem cell line. — SRN
Using immunocytochemistry, we show that these cells retain the characteristics of NSCs after more than 50 passages.
Under proliferation conditions, when supplemented with epidermal and basic fibroblast growth factors, the hc-NSCs expressed neural stem/progenitor cell markers like nestin, vimentin and Sox2.
When growth factors were withdrawn, proliferation and expression of v-myc and telomerase were dramatically reduced, and the hc-NSCs differentiated into glia and neurons (mostly glutamatergic and GABAergic, as well as tyrosine hydroxylase-positive, presumably dopaminergic neurons).
www.restorativeneurology.se /publications/Cacci2007   (357 words)

  
 Endoplasmic Reticulum-Golgi Intermediate Compartment Membranes and Vimentin Filaments Participate in Vaccinia Virus ...
Detection of vimentin with a specific monoclonal antibody shows its concentration in a perinuclear area (D), coincident with the localization of viral factories.
Vimentin filaments appear to enclose the VV factories, as can be observed in the merge picture (F).
The small 5-nm gold particles are associated to vimentin, while 10-nm gold particles are localized the VV core protein p39.
jvi.asm.org /cgi/content/full/76/4/1839   (9007 words)

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