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| | JIS: Tschinkel 2.12.2002 |
 | | Membranes were assayed utilizing a rabbit antiserum (1:500), raised against honeybee egg proteins (Bitondi and Simões, 1996), followed by incubation with a peroxidase-anti peroxidase system (Dako, Denmark, Z113) and diaminobenzidine (Vector SK-4100) as chromogen. |
 | | Western blot analyses indicated that in queen pupae treated with juvenile hormone, vitellogenin is expressed in PbmI, with increasing levels by Pbd, while in control pupae vitellogenin appeared only in PbmII, and remained at low levels (Fig. |
 | | The apparent ecdysteroid independence of the reproductive physiology of highly eusocial bees contrasts with observations on the primitively eusocial bumble bee, Bombus terrestris, where ecdysteroid titers are elevated in egg laying queens and in queenless workers (Bloch et al., 2000). |
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