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 | | Methylene chloride, administered to F344 male and female rats in their drinking water for 2 years, induced histological alterations of the liver at doses ò50 mg/kg/day; the no-observed- adverse effect level (NOAEL) for the study was 5 mg/kg/day (U.S. Based on these data, the U.S. calculated an oral RfD of 0.06 mg/kg/day for methylene chloride. |
 | | In inhalation studies with methylene chloride, cytoplasmic vacuolization and fatty infiltration of the liver and tubular degeneration and regenerative changes of the kidneys were observed in rats exposed continuously to 25 or 100 ppm of the chemical for 100 days (ATSDR 1993). |
 | | Rats exposed to 500 ppm methylene chloride, 6 hours/day, 5 days/week for 2 years developed multinucleated hepatocytes (the liver was not affected at 200 ppm), and increased hemosiderosis, cytomegaly, and cytoplasmic vacuolization of the liver after exposure to 1000 ppm, 6 hours/ day, 5 days/week for 2 years (ATSDR 1993). |
| www.epa.gov /opptintr/chemfact/s_dcm.txt (3192 words) |
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